breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 962,358 | G→C | V122L (GTT→CTT) | rpsA → | 30S ribosomal subunit protein S1 |
RA | 1,151,759 | G→A | E49K (GAA→AAA) | acpP → | acyl carrier protein (ACP) |
RA | 1,307,744 | Δ1 bp | coding (902/1461 nt) | clsA ← | cardiolipin synthase 1 |
MC JC | 1,673,557 | Δ120 bp | intergenic (‑56/‑237) | mdtJ ← / → tqsA | multidrug efflux system transporter/pheromone AI‑2 transporter |
MC JC | 1,674,769 | Δ12 bp | coding (857‑868/1035 nt) | tqsA → | pheromone AI‑2 transporter |
RA | 1,862,476 | T→C | intergenic (‑47/‑295) | msrB ← / → gapA | methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,610,107 | G→A | E541K (GAG→AAG) | hyfG → | hydrogenase 4, subunit |
RA | 3,247,487 | +A | intergenic (+59/‑286) | exuR → / → yqjA | hexuronate regulon transcriptional repressor; autorepressor/general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter |
JC | 3,395,612 | (TGGAGCC)1→2 | coding (647/3801 nt) | yhdP ← | DUF3971‑AsmA2 domains protein |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,187,667 | T→A | F773Y (TTC→TAC) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,213,051 | A→C | noncoding (12/120 nt) | rrfE → | 5S ribosomal RNA of rrnE operon |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423677–3424234 | 3424557–3424238 | 5–881 | 257 [251] | [252] 262 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 257907 | 0 (0.000) | 403 (1.060) | 210/284 | 0.0 | 99.9% | intergenic (+8/‑769) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | 258684 = | 1 (0.000) | pseudogene (9/331 nt) | crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 455 (1.160) | 217/292 | 0.0 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |