breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 214,925 A→C 65.5% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 214,931 A→C 62.6% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 705,870 A→G 50.0% intergenic (‑367/+128) ymcC ← / ← serX predicted outer membrane lipoprotein/tRNA‑Ser
RA 1,036,505 G→A 100% A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,356,166 G→C 5.0% L154V (CTA→GTA)  yehA ← predicted fimbrial‑like adhesin protein
RA 1,644,441 T→A 6.8% Q231H (CAA→CAT nlpB ← lipoprotein
RA 1,644,444 T→G 6.6% V230V (GTA→GTC nlpB ← lipoprotein
RA 1,644,452 T→A 5.9% M228L (ATG→TTG)  nlpB ← lipoprotein
RA 1,763,540 A→G 10.3% noncoding (304/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,556 T→C 24.3% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,558 G→C 7.6% noncoding (286/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,559 G→T 7.5% noncoding (285/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,580 G→T 6.2% noncoding (264/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,636 A→G 28.9% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,804,090 G→A 63.0% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,359,867 G→A 31.3% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,359,876 C→T 28.0% noncoding (1219/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,833,469 T→C 9.6% T261T (ACT→ACC groL → Cpn60 chaperonin GroEL, large subunit of GroESL
RA 2,833,483 C→A 8.3% T266N (ACC→AAC)  groL → Cpn60 chaperonin GroEL, large subunit of GroESL

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ Exported 252009 252062 54 11 [9] [10] 13 malZ maltodextrin glucosidase
* * ÷ Exported 865543 865589 47 11 [10] [7] 11 yciQ predicted inner membrane protein
* * ÷ Exported 899423 899459 37 11 [9] [10] 12 pspD peripheral inner membrane phage‑shock protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? Exported 202975 =NA (NA)3 (0.070) 3/94 NT NA noncoding (380/1542 nt) rrsH 16S ribosomal RNA
?Exported 202996 = NA (NA)noncoding (401/1542 nt) rrsH 16S ribosomal RNA
* ? Exported = 206681NA (NA)4 (0.090) 3/92 NT NA noncoding (2098/2904 nt) rrlH 23S ribosomal RNA
?Exported = 206690 NA (NA)noncoding (2107/2904 nt) rrlH 23S ribosomal RNA
* ? Exported = 21562223 (0.470)23 (1.020)
+27 bp
15/46 NT 58.5% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?Exported 2705182 = 48 (0.980)intergenic (+106/‑1) damX/dam hypothetical protein/DNA adenine methylase
* ? Exported 436259 =43 (0.880)42 (0.930) 27/92 NT 54.3% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?Exported = 2706018 31 (0.690)coding (836/837 nt) dam DNA adenine methylase
* ? Exported = 43683353 (1.080)79 (1.620) 40/100 NT 61.0% coding (546/546 nt) seqA regulatory protein for replication initiation
?Exported 1803907 = 48 (0.980)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? Exported 1109701 =33 (0.680)3 (0.070) 3/90 NT 9.7% coding (616/1290 nt) ydiS predicted oxidoreductase, FAD/NAD(P)‑binding domain
?Exported 1109736 = 26 (0.590)coding (651/1290 nt) ydiS predicted oxidoreductase, FAD/NAD(P)‑binding domain
* ? Exported = 182649663 (1.290)3 (0.080) 4/78 NT 6.0% intergenic (‑173/‑262) hydN/mutS formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/methyl‑directed mismatch repair protein
?Exported = 1826503 45 (1.180)intergenic (‑180/‑255) hydN/mutS formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/methyl‑directed mismatch repair protein
* ? Exported 1966484 =55 (1.130)5 (0.110) 3/92 NT 9.1% intergenic (‑91/+154) cmtA/tktA predicted fused mannitol‑specific enzyme IIBC component of PTS/transketolase 1, thiamin‑binding
?Exported 1966508 = 49 (1.090)intergenic (‑115/+130) cmtA/tktA predicted fused mannitol‑specific enzyme IIBC component of PTS/transketolase 1, thiamin‑binding
* ? Exported = 254058451 (1.040)5 (0.110) 3/90 NT 8.1% coding (75/699 nt) rfaY lipopolysaccharide core biosynthesis protein
?Exported = 2540601 68 (1.550)coding (92/699 nt) rfaY lipopolysaccharide core biosynthesis protein