breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 86,696 A→C Q356P (CAG→CCG)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 138,729 C→A intergenic (+96/+106) cueO → / ← gcd cuprous oxidase/multicopper oxidase with role in copper homeostasis/quinoprotein glucose dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 302,646 A→C intergenic (‑73/‑345) paoA ← / → yagU aldehyde dehydrogenase, Fe‑S subunit/inner membrane protein that contributes to acid resistance
RA 569,522 G→T R207S (AGG→AGT ybcK → DLP12 prophage; putative recombinase
RA 626,126 (A)5→4 coding (57/1611 nt) entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,458,851 G→T Q196H (CAG→CAT paaG → putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,715,754 G→T Q46K (CAA→AAA)  pdxY ← pyridoxal kinase 2
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,169,469 C→A S253* (TCG→TAG)  yegS → lipid kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,344,513 C→A intergenic (‑344/+81) yfaL ← / ← ypaB putative autotransporter adhesin YfaL/protein YpaB
RA 2,407,542 G→A intergenic (‑901/‑19) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,613,693 A→C L598L (CTA→CTC hyfR → DNA‑binding transcriptional activator HyfR
RA 2,920,925 G→T F392L (TTC→TTA gudP ← galactarate/glucarate/glycerate transporter GudP
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,730,681 G→T P29T (CCC→ACC)  xylA ← xylose isomerase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,866,031 G→T G81G (GGC→GGA yidE ← putative transport protein YidE
RA 3,963,423 C→A L269L (CTG→CTT gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 4,091,890 G→T F159L (TTC→TTA frvB ← putative PTS enzyme IIBC component FrvB
RA 4,113,614 G→T V108F (GTC→TTC)  uspD → universal stress protein D
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,609,294 G→T E217* (GAA→TAA)  yjjG → pyrimidine nucleotidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102962 3104055 1094 48 [47] [47] 49 [mutY] [mutY]
* * ÷ NC_000913 3423766–3424234 3424531–3424238 5–766 50 [45] [47] 51 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =86 (0.490)178 (1.140) 84/244 0.1 71.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 69 (0.440)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780586 (0.490)78 (0.500) 48/244 1.2 51.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 69 (0.440)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)277 (1.620) 104/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =355 (2.020)335 (1.920) 100/274 0.1 65.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)233 (1.320) 95/276 0.1 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA