breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 111,009 | 0 | G | T | 67.2% | 140.9 / 173.3 | 134 | intergenic (+25/‑35) | secA/mutT | protein translocation ATPase/8‑oxo‑dGTP diphosphatase |
* | NC_000913 | 4,604,344 | 0 | C | T | 37.5% | 170.7 / 86.1 | 136 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 2,326,450 | 0 | T | G | 33.7% | 166.1 / 74.1 | 104 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 2,001,918 | 0 | A | C | 31.7% | 235.8 / 57.9 | 123 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
* | NC_000913 | 3,556,207 | 0 | T | C | 29.4% | 115.3 / 27.0 | 68 | S215G (AGT→GGT) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 802,118 | 0 | A | C | 29.1% | 211.6 / 37.9 | 103 | T78P (ACC→CCC) | ybhI | putative tricarboxylate transporter |
* | NC_000913 | 4,568,504 | 0 | T | G | 28.9% | 415.1 / 32.2 | 203 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
* | NC_000913 | 3,206,435 | 0 | C | T | 28.2% | 181.8 / 55.4 | 103 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 2,628,625 | 0 | A | C | 27.0% | 157.5 / 28.6 | 74 | V105G (GTC→GGC) | pdeF | cyclic di‑GMP phosphodiesterase PdeF |
* | NC_000913 | 3,206,444 | 0 | T | A | 27.0% | 192.0 / 30.1 | 100 | intergenic (‑188/‑19) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 3,556,205 | 0 | A | C | 26.9% | 147.4 / 15.0 | 67 | S215R (AGT→AGG) ‡ | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 4,111,747 | 0 | G | T | 26.5% | 218.5 / 42.8 | 83 | N156K (AAC→AAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 1,775,467 | 0 | A | C | 25.7% | 161.0 / 12.9 | 74 | intergenic (+23/‑120) | aroD/ydiF | 3‑dehydroquinate dehydratase/putative acetate‑CoA transferase |
* | NC_000913 | 4,101,507 | 0 | T | G | 25.6% | 279.6 / 24.0 | 126 | intergenic (+77/‑183) | sodA/kdgT | superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter |
* | NC_000913 | 888,014 | 0 | T | G | 24.8% | 275.6 / 26.1 | 122 | noncoding (44/81 nt) | rybB | small regulatory RNA RybB |
* | NC_000913 | 445,558 | 0 | A | C | 24.3% | 150.2 / 32.0 | 70 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
* | NC_000913 | 1,130,379 | 0 | A | C | 23.6% | 373.4 / 22.6 | 161 | V162G (GTA→GGA) | flgA | flagellar basal body P‑ring formation protein FlgA |
* | NC_000913 | 4,101,514 | 0 | G | T | 23.3% | 326.6 / 53.7 | 120 | intergenic (+84/‑176) | sodA/kdgT | superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter |
* | NC_000913 | 4,296,060 | 0 | C | T | 23.1% | 436.3 / 119.1 | 208 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 29,496 | 0 | T | A | 22.7% | 207.6 / 33.4 | 88 | intergenic (+301/‑155) | dapB/carA | 4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha |
Marginal new junction evidence (lowest skew 10 of 23 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 374921 = | NA (NA) | 18 (0.150) | 12/190 | 5.7 | NA | intergenic (+40/‑538) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377531 = | NA (NA) | intergenic (+220/+4) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 374963 | NA (NA) | 19 (0.160) | 11/190 | 6.0 | NA | noncoding (33/34 nt) | other | REP31a |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375023 = | NA (NA) | 16 (0.130) | 10/192 | 6.5 | NA | intergenic (+142/‑436) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | NA (NA) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375248 = | NA (NA) | 18 (0.120) | 12/238 | 7.0 | NA | noncoding (15/34 nt) | other | REP31g |
? | NC_000913 | 377507 = | NA (NA) | noncoding (16/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | 375203 = | NA (NA) | 14 (0.100) | 9/216 | 7.5 | NA | noncoding (17/36 nt) | other | REP31f |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | 375102 = | NA (NA) | 13 (0.100) | 8/216 | 7.9 | NA | noncoding (17/36 nt) | other | REP31d |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | = 375160 | NA (NA) | 16 (0.090) +T |
9/274 | 8.9 | NA | noncoding (28/34 nt) | other | REP31e |
? | NC_000913 | = 377491 | NA (NA) | noncoding (32/34 nt) | other | REP32d | |||||
* | ? | NC_000913 | = 374963 | NA (NA) | 15 (0.090) | 8/268 | 9.2 | NA | noncoding (33/34 nt) | other | REP31a |
? | NC_000913 | = 377387 | NA (NA) | intergenic (+76/+148) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 374922 = | NA (NA) | 5 (0.040) | 4/202 | 9.6 | NA | intergenic (+41/‑537) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377430 = | NA (NA) | intergenic (+119/+105) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 2765420 = | 236 (1.380) | 3 (0.030) | 3/188 | 9.8 | 1.7% | intergenic (‑267/+93) | abpA/yfjM | CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM |
? | NC_000913 | = 2765411 | 179 (1.540) | intergenic (‑258/+102) | abpA/yfjM | CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM |