breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 345,403 G→T intergenic (+412/‑1) yahL → / → yahM uncharacterized protein YahL/uncharacterized protein YahM
RA 568,802 T→C I36I (ATT→ATC ylcJ → protein YlcJ
RA 697,054 (A)7→6 intergenic (‑7/+3) leuW ← / ← metT tRNA‑Leu/tRNA‑Met
RA 806,807 G→T P159T (CCT→ACT)  ybhC ← outer membrane lipoprotein YhbC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,399,671 G→T intergenic (‑145/+50) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,512,138 G→T S162I (AGC→ATC)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,542,254 G→T R87S (CGC→AGC)  cysU ← sulfate/thiosulfate ABC transporter inner membrane subunit CysU
RA 2,555,317 G→T L138M (CTG→ATG)  eutK ← putative structural protein, ethanolamine utilization microcompartment
RA 2,715,617 A→T A65A (GCA→GCT eamB → cysteine/O‑acetylserine exporter EamB
RA 2,769,535 C→A intergenic (+49/‑168) yfjQ → / → yfjR CP4‑57 prophage; protein YfjQ/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,943,207 G→T intergenic (‑62/‑130) ygdI ← / → csdA DUF903 domain‑containing lipoprotein YgdI/cysteine sulfinate desulfinase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,393,586 G→T G891G (GGC→GGA yhdP ← AsmA2 domain‑containing protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter
JC JC 4,543,608 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (494‑499/549 nt) fimA → type 1 fimbriae major subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211442 15198 71 [57] [58] 63 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102950 3104084 1135 59 [56] [57] 59 mutY mutY
* * ÷ NC_000913 3423767–3424234 3424540–3424239 6–774 62 [57] [57] 59 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)133 (0.800) 78/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =194 (1.130)191 (1.120) 73/274 0.5 65.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 9 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)161 (0.940) 78/276 0.4 98.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.010)coding (3907/4134 nt) rhsA rhs element protein RhsA