breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT44.3% 42.8 / 44.6 61S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_000913226,0410G.39.6% 503.3 / inf 235noncoding (283/2904 nt)rrlH23S ribosomal RNA
*NC_000913226,0420T.39.6% 465.6 / inf 235noncoding (284/2904 nt)rrlH23S ribosomal RNA
*NC_0009133,556,2070TC37.5% 66.7 / 31.1 56S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009133,556,2050AC36.8% 75.9 / 24.9 57S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,825,0610GA36.4% 42.1 / 20.2 33intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009133,206,4350CT35.9% 67.7 / 37.1 53intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009134,101,5070TG33.9% 95.2 / 17.0 63intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_000913650,8090TG31.5% 75.9 / 20.8 54T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009132,001,9180AC30.8% 82.1 / 18.3 52intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_000913372,6400TG29.8% 72.3 / 16.6 47L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009133,206,4440TA29.6% 90.1 / 27.2 54intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009134,111,7470GT28.8% 138.8 / 26.3 52N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,296,0600CT28.6% 161.5 / 66.7 91intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,568,5040TG28.3% 184.1 / 12.9 99T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_000913753,1250TA27.8% 121.4 / 18.6 54intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_0009132,326,4500TG27.8% 86.7 / 28.7 54G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009134,101,5140GT26.6% 175.5 / 18.9 64intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_00091329,4960TA23.8% 103.1 / 11.0 42intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009133,240,9850TG23.7% 119.3 / 12.6 59S348A (TCT→GCT) sstTserine/threonine:Na(+) symporter

Marginal new junction evidence (lowest skew 10 of 22 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)7 (0.120) 7/190 4.1 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 120780586 (1.050)12 (0.170) 10/244 4.2 14.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 72 (0.990)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 375102 =NA (NA)19 (0.300) 8/216 4.3 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 375246NA (NA)9 (0.120)
+GAACGCCTTATCCGGT
7/244 5.1 NA noncoding (13/34 nt) other REP31g
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 454269048 (0.580)6 (0.080) 6/258 5.7 10.1% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 62 (0.810)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 375059NA (NA)6 (0.070)
+T
6/274 6.0 NA noncoding (28/34 nt) other REP31c
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 374922 =NA (NA)4 (0.070) 3/202 6.1 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 4512054 =61 (0.740)4 (0.050) 4/256 6.6 5.2% coding (361/957 nt) fecD ferric citrate ABC transporter membrane subunit FecD
?NC_000913 4512149 = 88 (1.160)coding (266/957 nt) fecD ferric citrate ABC transporter membrane subunit FecD
* ? NC_000913 374922 =NA (NA)5 (0.060) 4/260 6.7 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)4 (0.050) 4/268 6.8 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377387 NA (NA)intergenic (+76/+148) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB