breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT46.5% 68.1 / 89.3 101S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_000913445,5580AC41.3% 49.6 / 51.2 63V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
*NC_0009133,556,2050AC39.0% 51.1 / 15.3 41S215R (AGT→AGGrtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,429,9840TG36.0% 101.4 / 36.1 75L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
*NC_0009134,111,7470GT34.2% 87.2 / 24.6 41N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009132,001,9180AC33.3% 112.7 / 47.4 81intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009134,568,5040TG31.4% 191.6 / 22.5 119T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_000913753,1250TA30.4% 139.4 / 29.9 69intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_000913650,8090TG30.1% 192.8 / 47.6 123T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009131,491,0980AC29.5% 100.3 / 16.4 61Y66S (TAC→TCC) cybBcytochrome b561
*NC_00091329,4960TA27.9% 133.5 / 28.1 61intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009133,926,3660AC27.6% 157.0 / 25.1 87G30G (GGT→GGGmioCflavoprotein MioC
*NC_0009131,825,0610GA27.5% 76.4 / 12.8 40intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009134,101,5070TG26.7% 175.1 / 10.3 90intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,296,0600CT26.4% 205.4 / 69.6 106intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,326,4500TG26.2% 101.2 / 33.8 61G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009133,404,2150TG26.1% 126.9 / 27.7 69G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_0009133,206,4350CT25.8% 123.2 / 26.6 62intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009131,335,6910TG25.7% 131.8 / 14.0 74intergenic (+24/‑140)ymiC/acnAprotein YmiC/aconitate hydratase 1
*NC_0009131,068,1730TG25.5% 194.7 / 30.4 106G31G (GGT→GGGymdFconserved protein YmdF

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 454269084 (0.880)16 (0.180) 16/258 3.7 14.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 115 (1.280)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =100 (1.040)9 (0.100) 8/258 6.0 7.9% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 117 (1.300)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 1979486 =118 (1.230)31 (0.320) 5/276 7.6 20.8% intergenic (‑271/‑267) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 555833107 (1.120)3 (0.040) 3/244 8.1 3.3% coding (1223/1386 nt) cysS cysteine‑‑tRNA ligase
?NC_000913 = 556784 82 (0.970)coding (90/213 nt) ybcJ putative RNA‑binding protein YbcJ
* ? NC_000913 = 89308968 (0.710)3 (0.030) 3/272 8.6 4.2% coding (133/477 nt) ybjN protein YbjN
?NC_000913 893093 = 71 (0.750)coding (137/477 nt) ybjN protein YbjN
* ? NC_000913 = 610860246 (2.560)3 (0.030) 3/272 8.6 1.2% coding (1635/2241 nt) fepA ferric enterobactin outer membrane transporter
?NC_000913 610864 = 246 (2.600)coding (1631/2241 nt) fepA ferric enterobactin outer membrane transporter
* ? NC_000913 = 1979489116 (1.210)14 (0.150) 3/276 8.7 10.8% intergenic (‑274/‑264) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 1397238NA (NA)5 (0.050) 3/276 8.7 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
* ? NC_000913 = 375160NA (NA)3 (0.030)
+T
3/274 8.7 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d