breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 866,148 Δ1 bp coding (217/1236 nt) moeA ← molybdopterin molybdotransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,585,592 C→G D299H (GAT→CAT)  ydeP ← putative oxidoreductase YdeP
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,023,726 A→C intergenic (+48/‑242) fliR → / → rcsA flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,327,395 G→A I90I (ATC→ATT rlmE ← 23S rRNA 2'‑O‑ribose U2552 methyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 705867 705933 67 3 [2] [2] 3 [nagE] [nagE]
* * ÷ NC_000913 928488 928537 50 5 [2] [2] 3 cydC glutathione/L‑cysteine ABC exporter subunit CydC
* * ÷ NC_000913 993917 993985 69 3 [2] [2] 3 ssuB aliphatic sulfonate ABC transporter ATP binding subunit
* * ÷ NC_000913 1129248 1129323 76 3 [2] [2] 4 murJ putative lipid II flippase MurJ
* * ÷ NC_000913 1135227 1135278 52 3 [2] [2] 3 flgG flagellar basal‑body rod protein FlgG
* * ÷ NC_000913 1142519 1142546 28 3 [2] [2] 3 rne ribonuclease E
* * ÷ NC_000913 1196288 1211432 15145 3 [2] [2] 3 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1280363 1280422 60 3 [1] [2] 3 narG nitrate reductase A subunit alpha
* * ÷ NC_000913 1286972 1287134 163 3 [2] [2] 3 [ychS]–[tpr] [ychS],[rttR],[tpr]
* * ÷ NC_000913 3423796–3424231 3424523–3424238 8–728 3 [2] [2] 4 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)3 (0.150) 3/190 2.0 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 3363001 =54 (1.860)35 (1.220) 29/274 0.1 55.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.100)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)35 (1.210) 29/276 0.1 94.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA