breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,757,559 G→T D47Y (GAC→TAC)  lpp → murein lipoprotein
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
MC JC 2,005,750 Δ40 bp coding (38‑77/366 nt) fliT → flagellar biosynthesis protein FliT
RA 2,062,705 A→T C207S (TGT→AGT)  ldtA ← L,D‑transpeptidase ErfK
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,074,709 T→A T163S (ACC→TCC)  fumE ← fumarase E
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,933,874 A→C M33L (ATG→CTG)  rbsA → ribose ABC transporter ATP binding subunit
RA 4,293,106 (G)7→6 coding (1595/1659 nt) nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,438,138 IS1 (–) +8 bp coding (432‑439/939 nt) ytfI → protein YtfI
RA 4,526,997 C→G A181P (GCA→CCA)  sgcE ← KpLE2 phage‑like element; putative epimerase SgcE

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696744 696913 170 45 [43] [41] 46 glnW–[glnU] glnW,[glnU]
* * ÷ NC_000913 3423738–3424234 3424544–3424239 6–807 45 [40] [44] 47 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =39 (0.320)90 (0.840) 58/246 0.3 73.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 31 (0.290)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780539 (0.320)85 (0.800) 54/246 0.5 72.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 31 (0.290)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)144 (1.230) 79/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =201 (1.670)195 (1.630) 95/276 0.0 65.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)144 (1.190) 77/278 0.1 96.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA