breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT43.7% 83.2 / 75.0 103S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_000913836,7560TG33.3% 69.0 / 10.9 42F136V (TTT→GTT) hcxBhydroxycarboxylate dehydrogenase B
*NC_0009131,825,0610GA32.0% 77.3 / 14.1 50intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_000913650,8090TG30.5% 98.8 / 15.4 60T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009134,568,5040TG30.1% 241.8 / 21.3 134T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009134,111,7470GT29.5% 107.1 / 21.0 44N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_000913888,0140TG28.7% 156.7 / 29.6 87noncoding (44/81 nt)rybBsmall regulatory RNA RybB
*NC_000913802,1180AC28.6% 104.8 / 13.5 56T78P (ACC→CCC) ybhIputative tricarboxylate transporter
*NC_0009134,101,5070TG28.4% 155.9 / 15.5 82intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,429,9840TG26.8% 148.6 / 19.2 71L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
*NC_0009134,296,0600CT26.7% 230.9 / 91.1 135intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_00091329,4960TA26.6% 137.1 / 23.8 64intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009132,628,6250AC26.1% 93.0 / 11.4 46V105G (GTC→GGC) pdeFcyclic di‑GMP phosphodiesterase PdeF
*NC_0009133,404,2150TG25.8% 126.9 / 22.0 66G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_0009131,491,0980AC24.7% 196.1 / 11.0 94Y66S (TAC→TCC) cybBcytochrome b561
*NC_000913175,6940TG24.7% 177.1 / 13.0 85G196G (GGT→GGGclcAchloride:H(+) antiporter ClcA
*NC_0009132,667,1260AC23.2% 183.4 / 11.8 84Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_0009133,531,4400AC22.9% 270.9 / 24.0 119Y334D (TAT→GAT) yhgEputative transport protein YhgE
*NC_000913646,7470TG22.8% 124.4 / 18.0 57T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
*NC_0009133,404,2180TG22.7% 144.5 / 12.2 66G245G (GGT→GGGyhdHacrylyl‑CoA reductase

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =82 (0.880)16 (0.180) 14/260 4.1 15.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 103 (1.180)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269081 (0.870)11 (0.130) 9/260 5.5 11.1% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 101 (1.160)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 2728251NA (NA)8 (0.090) 6/268 6.8 NA noncoding (934/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 = 2728340 NA (NA)noncoding (845/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 = 60800674 (0.980)6 (0.080) 3/226 7.5 7.5% intergenic (+18/‑59) hokE/insL‑2 protein HokE/IS186/IS421 transposase
?NC_000913 = 609351 NA (NA)noncoding (1345/1345 nt) IS186 repeat region
* ? NC_000913 4530896 =140 (1.500)5 (0.060) 3/262 8.2 3.5% coding (756/1122 nt) sgcX KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain
?NC_000913 4530971 = 147 (1.680)coding (681/1122 nt) sgcX KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain
* ? NC_000913 = 83944067 (0.720)3 (0.030) 3/268 8.3 4.7% coding (2092/2283 nt) fiu putative iron siderophore outer membrane transporter
?NC_000913 839628 = 56 (0.620)coding (1904/2283 nt) fiu putative iron siderophore outer membrane transporter
* ? NC_000913 226598 =74 (0.790)4 (0.040) 3/268 8.3 5.3% noncoding (840/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 226697 = NA (NA)noncoding (939/2904 nt) rrlH 23S ribosomal RNA
* ? NC_000913 = 364288776 (0.820)3 (0.030) 3/274 8.4 3.8% coding (246/753 nt) rsmJ 16S rRNA m(2)G1516 methyltransferase
?NC_000913 3642891 = 76 (0.830)coding (242/753 nt) rsmJ 16S rRNA m(2)G1516 methyltransferase
* ? NC_000913 = 443041078 (0.840)3 (0.030) 3/276 8.5 3.3% coding (547/1413 nt) cycA serine/alanine/glycine/cycloserine:H(+)symporter
?NC_000913 = 4430517 97 (1.050)coding (654/1413 nt) cycA serine/alanine/glycine/cycloserine:H(+)symporter
* ? NC_000913 = 286720180 (0.860)3 (0.030) 3/276 8.5 3.5% coding (351/993 nt) rpoS RNA polymerase, sigma S (sigma 38) factor
?NC_000913 = 2867282 88 (0.950)coding (270/993 nt) rpoS RNA polymerase, sigma S (sigma 38) factor