breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,403 A→G Y356C (TAC→TGC)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1
MC JC 257,908 Δ776 bp [crl] [crl]
RA 450,073 C→T W190* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 822,421 C→T L26L (TTG→TTA ybhN ← UPF0104 family inner membrane protein
RA 933,022 A→G D143G (GAC→GGC)  lrp → leucine‑responsive global transcriptional regulator
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
JC JC 1,673,670 IS1 (–) +9 bp intergenic (‑169/‑235) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter
RA 1,818,103 A→T V133E (GTA→GAA)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 2,492,323 IS1 (–) +9 bp coding (924‑932/1251 nt) frc ← formyl‑CoA transferase, NAD(P)‑binding
RA 2,907,364 A→T L193Q (CTG→CAG)  eno ← enolase
RA 2,907,452 C→G V164L (GTT→CTT)  eno ← enolase
RA 3,060,146 C→A Q132K (CAG→AAG)  argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,471,274 C→A G19V (GGC→GTC)  tufA ← translation elongation factor EF‑Tu 1
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,966,174 IS1 (–) +9 bp intergenic (+84/‑235) trxA → / → rho thioredoxin 1/transcription termination factor
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,430,521 A→G I220V (ATA→GTA)  cycA → D‑alanine/D‑serine/glycine transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1896925 1898281 1357 8 [7] [0] 14 [sdaA] [sdaA]
* * ÷ NC_000913 2684266 2685547 1282 9 [5] [7] 9 [glyA] [glyA]
* * ÷ NC_000913 2929579 2930928 1350 13 [0] [0] 12 sdaB L‑serine dehydratase 2
* * ÷ NC_000913 3258300 3259646 1347 11 [0] [0] 16 tdcG L‑serine dehydratase 3, anaerobic
* * ÷ NC_000913 3423785–3424233 3424513–3424239 7–729 8 [5] [7] 8 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 228016 =NA (NA)7 (0.160) 6/282 2.8 NA noncoding (2258/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 228041 = NA (NA)noncoding (2283/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 381292 =NA (NA)8 (0.180) 8/278 2.2 NA noncoding (33/1331 nt) IS2 repeat region
?NC_000913 381312 = NA (NA)noncoding (53/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =11 (0.240)44 (1.060) 32/264 0.1 82.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 9 (0.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780511 (0.240)30 (0.730) 24/264 0.4 76.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 9 (0.220)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1432648NA (NA)8 (0.180) 6/280 2.8 26.1% coding (238/576 nt) tfaR Rac prophage; putative tail fiber assembly protein
?NC_000913 1634576 = 24 (0.520)coding (310/576 nt) tfaQ Qin prophage; putative tail fibre assembly protein
* ? NC_000913 1615598 =41 (0.880)2137 (48.040) 179/284 0.0 98.4% coding (795/882 nt) yneJ putative DNA‑binding transcriptional regulator
?NC_000913 = 1625301 31 (0.700)intergenic (+10/+34) ydeJ/dcp inactive PncC family protein/dipeptidyl carboxypeptidase II
* ? NC_000913 3060398 =31 (0.670)8 (0.180) 8/280 2.3 21.5% coding (646/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
?NC_000913 3060457 = 29 (0.660)coding (705/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
* ? NC_000913 = 352940531 (0.670)8 (0.190) 7/276 2.5 21.9% coding (58/402 nt) hslR ribosome‑associated heat shock protein Hsp15
?NC_000913 = 3529461 28 (0.650)coding (114/402 nt) hslR ribosome‑associated heat shock protein Hsp15
* ? NC_000913 3764005 =32 (0.690)11 (0.250) 6/280 2.8 21.3% coding (1823/4134 nt) rhsA Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
?NC_000913 3764057 = 51 (1.160)coding (1875/4134 nt) rhsA Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
* ? NC_000913 4498181 =NA (NA)49 (1.060) 11/296 1.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD1/yjgX IS2 transposase TnpB/pseudogene fragment