breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 118 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA64.7% 3.1 / 28.3 34intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_0009131,982,5130CG63.2% 11.7 / 42.5 39intergenic (‑126/+41)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
*NC_000913993,9170AC63.0% 14.3 / 28.5 27V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
*NC_0009132,984,4410AC62.1% 19.9 / 31.2 30G384G (GGT→GGGyqeFshort chain acyltransferase
*NC_00091375,0790CT60.0% 4.2 / 47.7 40L134L (CTG→CTAthiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009133,035,7570AC59.2% 30.5 / 54.4 49G150G (GGT→GGGprfBpeptide chain release factor RF‑2
*NC_000913599,5760TG58.3% 9.9 / 25.2 24V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009131,079,4680AG58.3% 4.7 / 23.8 24D55G (GAT→GGT) putPproline:sodium symporter
*NC_000913357,8800TC58.1% 8.4 / 30.7 31K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,613,9040AC56.0% 18.8 / 29.0 25V267G (GTA→GGA) sadsuccinate semialdehyde dehydrogenase, NAD(P)+‑dependent
*NC_0009131,356,2010TG55.0% 6.5 / 16.0 20T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
*NC_0009133,177,5310AC54.8% 34.6 / 45.3 31V127G (GTG→GGG) nudFADP‑ribose pyrophosphatase
*NC_000913300,7110GC54.3% 13.1 / 41.3 35P75R (CCG→CGG) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_000913354,2880TG53.3% 42.6 / 59.1 45V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_00091375,0740TG52.5% 39.7 / 56.0 40N136T (AAC→ACC) thiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
*NC_0009131,982,5510GA52.5% 13.2 / 48.1 40intergenic (‑164/+3)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
*NC_0009132,337,7230AC52.5% 46.9 / 65.3 40G566G (GGT→GGGgyrADNA gyrase (type II topoisomerase), subunit A
*NC_0009131,982,5230AG52.4% 27.4 / 47.8 42intergenic (‑136/+31)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
*NC_0009132,903,6920CA51.1% 34.8 / 74.3 45intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
*NC_0009131,129,3030AG50.0% 9.5 / 18.5 26I489V (ATT→GTT) murJputative lipid II flippase

Marginal new junction evidence (lowest skew 10 of 103 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16169051565 (33.760)40 (0.930) 23/276 0.5 2.6% intergenic (+27/‑123) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
?NC_000913 = 1616917 1563 (36.160)intergenic (+39/‑111) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
* ? NC_000913 1616896 =1566 (33.780)21 (0.490) 18/276 0.8 1.4% intergenic (+18/‑132) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
?NC_000913 1616928 = 1563 (36.160)intergenic (+50/‑100) yneK/ydeA uncharacterized protein/arabinose efflux transporter, arabinose‑inducible
* ? NC_000913 = 16223331678 (36.190)16 (0.360) 11/280 1.7 0.91% coding (1002/1188 nt) ydeE putative transporter
?NC_000913 = 1622355 1882 (42.910)coding (1024/1188 nt) ydeE putative transporter
* ? NC_000913 3927418 =70 (1.510)8 (0.190) 7/272 2.4 12.6% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 47 (1.100)coding (303/993 nt) asnA asparagine synthetase A
* ? NC_000913 = 16227781437 (31.000)9 (0.210) 7/276 2.5 0.62% intergenic (+37/+182) yneM/dgcZ inner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
?NC_000913 = 1622789 1531 (35.420)intergenic (+48/+171) yneM/dgcZ inner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
* ? NC_000913 4409174 =NA (NA)10 (0.240) 6/266 2.6 11.8% noncoding (22/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 4409235 = 75 (1.800)noncoding (83/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 1242577 =36 (0.780)5 (0.120) 5/260 2.9 12.9% coding (504/915 nt) ldcA murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
?NC_000913 1242595 = 36 (0.880)coding (486/915 nt) ldcA murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
* ? NC_000913 197975 =59 (1.270)5 (0.120) 5/268 2.9 9.1% coding (48/2433 nt) bamA BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
?NC_000913 197989 = 47 (1.120)coding (62/2433 nt) bamA BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
* ? NC_000913 4516891 =37 (0.800)5 (0.110) 5/278 3.0 13.0% coding (827/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
?NC_000913 4516925 = 32 (0.730)coding (793/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
* ? NC_000913 4409625 =59 (1.270)6 (0.140) 5/276 3.0 10.5% coding (351/732 nt) rlmB 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
?NC_000913 4409708 = 47 (1.090)coding (434/732 nt) rlmB 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent