breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 33.3% intergenic (+45/+63) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,817 A→T 35.5% intergenic (+48/+60) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,850 C→T 46.1% intergenic (+81/+27) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,858 T→A 50.0% intergenic (+89/+19) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,876 C→T 47.0% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,913 C→G 35.2% S213S (TCG→TCC tpn ← IS431mec transposase
RA 35,940 G→A 34.5% R204R (CGC→CGT tpn ← IS431mec transposase
RA 35,970 T→C 53.9% E194E (GAA→GAG tpn ← IS431mec transposase
RA 35,997 T→C 31.0% T185T (ACA→ACG tpn ← IS431mec transposase
RA 36,015 C→T 30.8% R179R (AGG→AGA tpn ← IS431mec transposase
RA 36,087 A→G 58.1% C155C (TGT→TGC tpn ← IS431mec transposase
RA 36,119 C→T 29.4% V145I (GTA→ATA)  tpn ← IS431mec transposase
RA 36,124 G→A 55.4% A143V (GCT→GTT)  tpn ← IS431mec transposase
RA 36,133 A→G 27.5% V140A (GTA→GCA)  tpn ← IS431mec transposase
RA 36,161 T→C 28.5% I131V (ATT→GTT)  tpn ← IS431mec transposase
RA 36,174 T→C 28.9% K126K (AAA→AAG tpn ← IS431mec transposase
RA 36,269 C→T 58.8% A95T (GCA→ACA)  tpn ← IS431mec transposase
RA 36,315 G→A 63.0% Y79Y (TAC→TAT tpn ← IS431mec transposase
RA 36,378 C→T 89.2% L58L (TTG→TTA tpn ← IS431mec transposase
RA 36,413 C→T 62.7% V47I (GTC→ATC)  tpn ← IS431mec transposase
RA 36,444 C→T 52.2% R36R (AGG→AGA tpn ← IS431mec transposase
RA 36,471 A→G 23.9% S27S (AGT→AGC tpn ← IS431mec transposase
RA 76,265 C→T 21.4% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 69.5% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 65.3% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 65.8% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 111,516 C→A 36.8% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 222,846 C→A 21.0% G148V (GGG→GTG)  glcB ← PTS family glucose/glucoside (glc) porter component IIABC
RA 1,306,605 G→T 33.3% G223V (GGA→GTA)  fabG → short chain dehydrogenase
RA 1,354,081 C→A 25.0% E242* (GAA→TAA)  USA300HOU_1269 ← hypothetical protein
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,929,812 G→A 26.6% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 32.0% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 30.8% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 32.0% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,862:1 +T 28.0% intergenic (‑945/‑223) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 28.1% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 33.3% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 33.3% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 33.3% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 33.4% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 33.3% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 33.3% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 26.1% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 26.1% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,010,695 T→A 33.5% L35L (CTA→CTT ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,139,469 T→C 30.8% N31S (AAC→AGC)  xtmA ← terminase small subunit
RA 2,139,474 T→A 30.4% G29G (GGA→GGT xtmA ← terminase small subunit
RA 2,139,498 T→C 30.8% V21V (GTA→GTG xtmA ← terminase small subunit
RA 2,139,504 C→A 36.3% L19L (CTG→CTT xtmA ← terminase small subunit
RA 2,139,513 A→T 36.4% I16I (ATT→ATA xtmA ← terminase small subunit
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP000730 463494 463512 19 2 [1] [1] 2 lpl11 possible lipoprotein
* * ÷ CP000730 983737 983785 49 2 [1] [0] 2 USA300HOU_0938/eap hypothetical membrane protein/possible extracellular adherence protein Eap
* * ÷ CP000730 983976 984019 44 2 [1] [1] 2 USA300HOU_0938/eap hypothetical membrane protein/possible extracellular adherence protein Eap
* * ÷ CP000730 1043361 1043390 30 2 [0] [1] 3 USA300HOU_0984/USA300HOU_0985 glycosyltransferase/possible iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein
* * ÷ CP000730 1045148 1045210 63 2 [0] [1] 3 USA300HOU_0987/USA300HOU_0988 hypothetical protein/hypothetical protein
* * ÷ CP000730 1072623 1072628 6 3 [1] [1] 3 folD bifunctional methylenetetrahydrofolate dehydrogenase (NADP(+))/methenyltetrahydrofolate cyclohydrolase
* * ÷ CP000730 1166331 1166471 141 2 [1] [0] 2 [USA300HOU_1093] [USA300HOU_1093]
* * ÷ CP000730 1248291 1248329 39 2 [1] [1] 2 smc SMC superfamily ATP‑binding chromosome segregation protein
* * ÷ CP000730 1320703 1320717 15 2 [1] [0] 2 mutS1 DNA mismatch repair protein MutS
* * ÷ CP000730 1337231 1337284 54 2 [1] [1] 2 glnA glutamate‑‑ammonia ligase
* * ÷ CP000730 1345403 1345583 181 2 [1] [1] 2 [USA300HOU_1257] [USA300HOU_1257]
* * ÷ CP000730 1400136 1400194 59 2 [1] [1] 2 [umuC] [umuC]
* * ÷ CP000730 1515745 1515806 62 2 [1] [1] 2 [ypsC] [ypsC]
* * ÷ CP000730 1655866 1655923 58 2 [1] [1] 2 pulF competence protein ComGB
* * ÷ CP000730 1894340 1894397 58 2 [1] [1] 2 USA300HOU_1755/putA lysophospholipase/proline dehydrogenase
* * ÷ CP000730 2203636 2203696 61 3 [1] [0] 2 [murF] [murF]