breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,876 C→T 42.2% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,913 C→G 18.6% S213S (TCG→TCC tpn ← IS431mec transposase
RA 35,940 G→A 33.1% R204R (CGC→CGT tpn ← IS431mec transposase
RA 35,970 T→C 52.3% E194E (GAA→GAG tpn ← IS431mec transposase
RA 35,997 T→C 36.4% T185T (ACA→ACG tpn ← IS431mec transposase
RA 36,015 C→T 35.2% R179R (AGG→AGA tpn ← IS431mec transposase
RA 36,087 A→G 55.3% C155C (TGT→TGC tpn ← IS431mec transposase
RA 36,119 C→T 35.0% V145I (GTA→ATA)  tpn ← IS431mec transposase
RA 36,124 G→A 54.2% A143V (GCT→GTT)  tpn ← IS431mec transposase
RA 36,133 A→G 34.4% V140A (GTA→GCA)  tpn ← IS431mec transposase
RA 36,161 T→C 35.9% I131V (ATT→GTT)  tpn ← IS431mec transposase
RA 36,174 T→C 39.6% K126K (AAA→AAG tpn ← IS431mec transposase
RA 36,269 C→T 71.4% A95T (GCA→ACA)  tpn ← IS431mec transposase
RA 36,315 G→A 69.4% Y79Y (TAC→TAT tpn ← IS431mec transposase
RA 36,378 C→T 87.8% L58L (TTG→TTA tpn ← IS431mec transposase
RA 36,413 C→T 57.9% V47I (GTC→ATC)  tpn ← IS431mec transposase
RA 36,444 C→T 47.7% R36R (AGG→AGA tpn ← IS431mec transposase
RA 36,581 C→T 54.4% intergenic (‑30/‑493) tpn ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,265 C→T 28.1% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 64.1% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 65.3% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 59.7% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 512,643 A→G 58.8% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,793,181 T→A 28.6% S222S (TCA→TCT polA ← DNA‑directed DNA polymerase I
RA 1,860,581 A→G 39.9% L1198S (TTG→TCG)  sftA ← DNA translocase
RA 1,929,812 G→A 52.6% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 68.4% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 68.9% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 59.1% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,862:1 +T 50.0% intergenic (‑945/‑223) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 50.3% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 58.5% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 59.4% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 59.6% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 59.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 59.2% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 56.7% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 50.0% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 50.3% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 43.8% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 43.7% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 43.9% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 2,187,071 C→T 100% R331K (AGA→AAA)  rsbU ← sigma factor B regulator
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,373,835 C→T 100% intergenic (‑60/+53) pbuG ← / ← topB NCS2 family nucleobase:cation symporter‑2/DNA topoisomerase TopB
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP000730 691525 691587 63 2 [0] [1] 3 USA300HOU_0634 hypothetical protein
* * ÷ CP000730 790584 790601 18 2 [0] [1] 2 kipA/ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
* * ÷ CP000730 964055 964089 35 2 [1] [1] 2 rexB ATP‑dependent nuclease Rex subunit B
* * ÷ CP000730 1237537 1237586 50 2 [1] [1] 2 asp hypothetical protein
* * ÷ CP000730 1242418 1242429 12 2 [1] [1] 2 fapR hypothetical protein
* * ÷ CP000730 1253719 1253755 37 2 [1] [1] 2 ftsY/yfhO Sec family Type I general secretory pathway protein signal recognition particle protein FtsY/hypothetical membrane protein
* * ÷ CP000730 1360588 1360621 34 2 [1] [1] 2 [thrB] [thrB]
* * ÷ CP000730 1461840 1461906 67 2 [1] [1] 2 [ctpA] [ctpA]
* * ÷ CP000730 1608032 1608089 58 2 [1] [1] 2 USA300HOU_1492 possible lipoprotein
* * ÷ CP000730 1639205 1639273 69 2 [1] [1] 2 argR/ispA arginine repressor/geranyltranstransferase
* * ÷ CP000730 1649069 1649111 43 2 [1] [1] 2 yqhL/gcvPB possible rhodanese domain sulfurtransferase/glycine dehydrogenase (decarboxylating) subunit 2
* * ÷ CP000730 1780609 1780697 89 2 [1] [1] 2 tig/lysP peptidylprolyl isomerase/APC family amino acid‑polyamine‑organocation transporter
* * ÷ CP000730 1909775 1909843 69 2 [1] [1] 2 crcB camphor resistance protein CrcB
* * ÷ CP000730 2056299 2056304 6 2 [1] [1] 2 sspP C47 family staphopain protease
* * ÷ CP000730 2176705–2181145 2181145 1–4441 4 [0] [1] 3 [rrl4]–[rrs4] [rrl4],[rrs4]
* * ÷ CP000730 2726894 2726899 6 2 [0] [0] 2 USA300HOU_2581/USA300HOU_2582 hypothetical protein/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =6 (0.610)3 (0.440)
+40 bp
3/180 NT 30.3% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = 14 (1.430)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA