breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,378 C→A D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 367,644 G→T intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator
RA 463,014 C→A E47* (GAA→TAA)  USA300HOU_0437 ← hypothetical protein
RA 469,786 A→G G118G (GGA→GGG USA300HOU_0445 → hypothetical protein
RA 479,553 G→A A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 638,614 G→T T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 707,879 A→T L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A intergenic (+277/‑197) kipA → / → ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 1,232,465 G→C A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,454 C→T G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,797,772 G→C R7G (CGC→GGC)  phoR ← sensor histidine kinase
JC 1,909,272 Δ48 bp coding (79‑126/303 nt) USA300HOU_1771 → hypothetical membrane protein
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,021,817 C→T V139I (GTT→ATT)  ykhA ← acyl‑CoA hydrolase
RA 2,027,751 C→G V66L (GTA→CTA)  vraS ← sensor histidine kinase VraS
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,252 C→A A80S (GCT→TCT)  ybbP ← hypothetical membrane protein
RA 2,360,799 A→G G66G (GGT→GGC adk ← adenylate kinase
RA 2,372,863 C→G G305R (GGA→CGA)  pbuG ← NCS2 family nucleobase:cation symporter‑2
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,679,710 G→T G65V (GGC→GTC)  cbpD → possible secretory antigen
RA 2,709,297 T→A K544* (AAA→TAA)  oatA ← possible acetyltransferase
RA 2,856,087 T→A P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =102 (1.260)54 (0.940)
+40 bp
44/196 0.3 42.7% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA