breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 444,755 G→T 60.3% E25* (GAA→TAA)  yajQ → phage Phi6 host factor, ATP/GTP binding protein
JC JC 572,356 IS2 (–) +5 bp 54.1% intergenic (+105/‑8) ybcM → / → ylcH DLP12 prophage; putative DNA‑binding transcriptional regulator/uncharacterized protein, DLP12 prophage
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,587,102 G→A 7.0% C211C (TGC→TGT ydeQ ← putative fimbrial‑like adhesin protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,334,502 C→A 10.1% R265S (CGC→AGC)  ispB → octaprenyl diphosphate synthase
RA 3,436,447 G→A 10.4% G414D (GGT→GAT)  rsmB → 16S rRNA m(5)C967 methyltransferase, SAM‑dependent
RA 3,560,113 A→C 12.1% pseudogene (341/606 nt) glpR ← pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,560,329 G→T 49.9% pseudogene (125/606 nt) glpR ← pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,560,460 A→T 20.4% pseudogene (146/150 nt) glpR ← pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,812,597 C→T 9.4% intergenic (‑38/‑134) yicR ← / → dfp UPF0758 family protein/fused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
RA 3,815,809 +A 13.5% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,950,469 T→G 22.9% intergenic (+49/‑38) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein
RA 4,116,706 T→G 100% K173Q (AAA→CAA)  glpK ← glycerol kinase
RA 4,187,507 A→C 100% N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,268 T→C 29.3% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2469148 2469769 622 11 [10] [10] 11 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ NC_000913 2903652 2903944 293 11 [9] [10] 11 ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =17 (0.370)20 (0.470) 16/518 NT 56.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 15 (0.350)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780515 (0.330)18 (0.420) 17/518 NT 55.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 15 (0.350)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related