breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
JC JC 528,897 IS5 (+) +4 bp 42.5% pseudogene (258‑261/491 nt) ybbD → pseudogene
RA 1,097,158 C→T 17.3% intergenic (+329/+407) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,466,210 G→A 6.1% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,585 T→C 5.6% pseudogene (1194/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,708,805 T→C 5.9% R629R (CGT→CGC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 6.3% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,847 G→A 8.1% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,930 C→A 8.4% P671Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,958 T→C 5.8% V680V (GTT→GTC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,304,523 G→C 8.1% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,537 A→G 8.4% intergenic (+144/+571) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,540 C→T 5.4% intergenic (+147/+568) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,762 T→C 5.0% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC 3,815,859 Δ82 bp 89.9% [rph][rph] [rph], [rph]
RA 3,875,532 A→T 13.8% H32L (CAT→CTT)  yidX → putative lipoprotein
RA 4,116,407 C→A 100% M272I (ATG→ATT glpK ← glycerol kinase
RA 4,183,258 G→A 100% E672K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,296,190 A→G 20.2% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 25.6% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 25.4% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 12.0% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 7.2% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 7.5% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
JC 4,396,100 Δ3 bp 1.8% coding (36‑38/1338 nt) amiB → N‑acetylmuramoyl‑l‑alanine amidase II

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 527117 529137 2021 67 [64] [63] 69 [rhsD]–ybbD [rhsD], ybbC, rhsH, ybbD
* * ÷ NC_000913 582640 584723 2084 69 [64] [62] 71 [ybcY]–[ompT] [ybcY], ybcY, tfaX, appY, [ompT]
* * ÷ NC_000913 1464965 1465439 475 68 [66] [65] 67 [paaY]–[ydbA] [paaY], ynbG, [ydbA]
* * ÷ NC_000913 1529922 1530558 637 68 [66] [66] 67 [rhsE]–[ydcD] [rhsE], [ydcD]
* * ÷ NC_000913 1978258 1979791 1534 67 [66] [66] 69 insB1–[uspC] insB1, insA, [uspC]
* * ÷ NC_000913 2033398 2034181 784 68 [66] [65] 67 [yedR]–[yedS] [yedR], [yedS]
* * ÷ NC_000913 2105097 2107448 2352 67 [64] [66] 74 [wbbI]–[glf] [wbbI], wbbH, [glf]
* * ÷ NC_000913 2455522 2456217 696 69 [66] [64] 67 [yfcV] [yfcV]
* * ÷ NC_000913 2469004 2470775 1772 70 [66] [65] 67 [gtrB]–gtrS [gtrB], gtrS
* * ÷ NC_000913 2774249 2775311 1063 67 [66] [66] 67 [yfjW] [yfjW]
* * ÷ NC_000913 2785786 2786943 1158 67 [66] [66] 68 [ileY]–[ygaQ] [ileY], [ygaQ]
* * ÷ NC_000913 2903539 2904114 576 67 [66] [64] 70 ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
* * ÷ NC_000913 2994854 2995999 1146 67 [60] [66] 69 [ygeK]–[ygeO] [ygeK], ygeN, [ygeN], [ygeO]
* * ÷ NC_000913 3581611 3582293 683 75 [60] [65] 68 [yhhY]–[yhhZ] [yhhY], [yhhZ]
* * ÷ NC_000913 3804072 3805230 1159 68 [66] [64] 67 [waaB]–[waaP] [waaB], waaS, [waaP]
* * ÷ NC_000913 4556312 4556814 503 67 [57] [66] 67 [yjiC] [yjiC]

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 112281 =169 (1.250)4 (0.030) 4/540 NT 2.4% coding (319/744 nt) zapD FtsZ stabilizer
?NC_000913 112312 = 159 (1.200)coding (288/744 nt) zapD FtsZ stabilizer
* ? NC_000913 1207790 =79 (0.590)58 (0.460) 49/520 NT 47.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 52 (0.410)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780574 (0.550)81 (0.640) 56/520 NT 57.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 52 (0.410)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1293199137 (1.010)4 (0.030) 4/552 NT 2.8% intergenic (‑277/‑328) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 1299499 = NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 12994980 (0.000)22 (0.170) 20/544 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)22 (0.170) 20/536 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 355899437 (0.270)99 (0.760) 66/532 NT 70.1% coding (727/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
?NC_000913 3560035 = 49 (0.380)pseudogene (419/606 nt) glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
* ? NC_000913 4409174 =NA (NA)4 (0.030) 4/522 NT 4.0% noncoding (22/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 4409235 = 95 (0.740)noncoding (83/98 nt) RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP331 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site