breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 259 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,480,3710TG52.1% 52.6 / 73.1 48G390G (GGT→GGGdsdAD‑serine dehydratase
*NC_0009134,538,1090AC49.1% 71.6 / 21.9 58G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009134,332,7910AC46.0% 104.8 / 17.8 63V161G (GTG→GGG) basSsensory histidine kinase in two‑component regulatory system with BasR
*NC_0009131,415,8060GT44.7% 59.1 / 16.6 38N527K (AAC→AAArecERac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
*NC_0009134,111,7470GT44.4% 84.6 / 29.3 54N156K (AAC→AAAyiiQDUF1454 family putative periplasmic protein
*NC_0009134,381,7280AC44.4% 83.1 / 11.1 54G197G (GGT→GGGfrdAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
*NC_0009132,958,7710AT44.2% 69.2 / 30.3 52D38E (GAT→GAAptrAprotease III
*NC_0009134,302,6580TG44.0% 60.5 / 12.5 50T141P (ACC→CCC) mdtOmembrane translocase (MDR) of MdtNOP efflux pump, PET family
*NC_0009135,1590GA43.2% 52.0 / 21.1 45intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
*NC_000913925,9000AG43.1% 81.3 / 12.9 51noncoding (72/88 nt)serWtRNA‑Ser
*NC_0009131,222,2330GA42.9% 27.9 / 12.2 28pseudogene (60/75 nt)ycgHpseudogene; putative ATP‑binding component of a transport system
*NC_0009131,567,7380AT42.4% 40.9 / 17.7 34V362V (GTT→GTAyddWliprotein, glycosyl hydrolase homolog
*NC_0009132,511,7720AC42.2% 82.9 / 10.5 45V312G (GTG→GGG) mntHmanganese/divalent cation transporter
*NC_00091329,4960TA42.0% 78.5 / 26.3 50intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009133,206,4350CT41.9% 46.1 / 23.6 43intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009131,487,1630CT41.7% 61.3 / 23.3 48intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
*NC_0009134,394,5790GC41.3% 80.0 / 22.2 47G172R (GGC→CGC) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
*NC_0009131,383,7220GA41.2% 57.8 / 21.9 51F80F (TTC→TTTycjWLacI family putative transcriptional repressor
*NC_0009131,376,5050AC41.1% 107.1 / 10.4 56T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009131,312,2100CA40.5% 83.0 / 22.1 42V13F (GTT→TTT) yciAacyl‑CoA esterase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 120780530 (0.510)12 (0.230) 11/266 5.4 28.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 35 (0.670)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =26 (0.440)11 (0.210) 11/266 5.4 26.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 39 (0.750)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 4542682 =58 (0.990)3 (0.050) 3/280 9.9 5.0% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 59 (1.070)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)