breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 255,591 Δ18,364 bp IS5‑mediated [pepD][ykfC] 27 genes
RA 1,756,637 G→C E314Q (GAA→CAA)  pykF → pyruvate kinase I
RA 1,969,313 C→A E18* (GAG→TAG)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,037,332 G→T T274K (ACA→AAA)  yedV ← putative sensory kinase in two‑component regulatory system with YedW
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 3,327,665 IS5 (+) +4 bp intergenic (‑1/‑122) rlmE ← / → yhbY 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent/RNA binding protein associated with pre‑50S ribosomal subunits
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,182,938 A→C E565A (GAA→GCA)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423755–3424234 3424554–3424238 5–800 31 [28] [28] 29 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)50 (0.620) 46/290 1.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein