breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 563 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC50.0% 58.6 / 23.2 58K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009134,378,8020AC46.8% 124.6 / 18.5 79V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_0009131,222,2330GA46.5% 36.4 / 22.0 43pseudogene (60/75 nt)ycgHpseudogene; putative ATP‑binding component of a transport system
*NC_000913659,9400TA45.9% 70.0 / 44.0 61I93F (ATT→TTT) lipAlipoate synthase
*NC_000913354,2880TG45.4% 125.1 / 24.5 86V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913357,8900CA45.0% 57.8 / 33.3 60L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913606,6120GA44.6% 106.7 / 32.2 74Q258* (CAG→TAG) ybdKweak gamma‑glutamyl:cysteine ligase
*NC_0009133,556,2070TC44.0% 98.9 / 30.2 75S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009133,556,2000GA43.8% 97.1 / 42.5 80S217F (TCC→TTC) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,346,6750TG43.3% 157.7 / 27.8 99T218P (ACC→CCC) fumBanaerobic class I fumarate hydratase (fumarase B)
*NC_0009131,487,1630CT42.6% 62.1 / 29.2 54intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
*NC_0009134,112,5780TG42.3% 140.6 / 14.4 81G88G (GGT→GGGyiiRDUF805 family putative inner membrane protein
*NC_0009133,508,6570AG41.2% 111.5 / 21.6 80F386S (TTC→TCC) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
*NC_0009133,026,9410AC41.2% 146.6 / 12.3 86E392D (GAA→GACguaDguanine deaminase
*NC_0009132,480,3710TG40.7% 143.4 / 12.7 81G390G (GGT→GGGdsdAD‑serine dehydratase
*NC_0009132,921,1730TC40.7% 129.3 / 25.0 81I310V (ATT→GTT) gudPputative D‑glucarate transporter
*NC_0009133,035,7570AC40.7% 147.1 / 19.6 81G150G (GGT→GGGprfBpeptide chain release factor RF‑2
*NC_0009132,578,1430AC40.5% 136.8 / 21.7 79L79V (TTA→GTA) maeBmalic enzyme: putative oxidoreductase/phosphotransacetylase
*NC_0009132,349,1450AC40.3% 120.8 / 16.9 67F110V (TTC→GTC) inaAacid‑inducible Kdo/WaaP family putative kinase
*NC_0009132,151,5890AT40.0% 68.5 / 24.4 50L135F (TTA→TTTyegJuncharacterized protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)17 (0.220) 17/282 4.9 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
* ? NC_000913 = 120780559 (0.710)14 (0.190) 14/266 5.3 21.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 49 (0.660)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =58 (0.700)10 (0.140) 10/266 6.5 16.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 52 (0.700)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 925901 =54 (0.650)13 (0.160)
+C
11/296 6.8 20.9% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 45 (0.540)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser