breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
MC JC | 255,591 | Δ18,364 bp | IS5‑mediated | [pepD]–[ykfC] | 27 genes [pepD], gpt, frsA, crl, crl, phoE, proB, proA, thrW, ykfN, ykfI, yafW, ykfH, ykfG, yafX, ykfF, ykfB, yafY, ykfL, ykfK, yafZ, ykfA, perR, insN, insI1, insN, [ykfC] |
RA | 1,756,637 | G→C | E314Q (GAA→CAA) | pykF → | pyruvate kinase I |
RA | 1,969,313 | C→A | E18* (GAG→TAG) | cheR ← | chemotaxis regulator, protein‑glutamate methyltransferase |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,037,332 | G→T | T274K (ACA→AAA) | yedV ← | putative sensory kinase in two‑component regulatory system with YedW |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
JC JC | 3,327,665 | IS5 (+) +4 bp | intergenic (‑1/‑122) | rlmE ← / → yhbY | 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent/RNA binding protein associated with pre‑50S ribosomal subunits |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,182,938 | A→C | E565A (GAA→GCA) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423755–3424234 | 3424554–3424238 | 5–800 | 31 [28] | [28] 29 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 50 (0.620) | 46/290 | 1.1 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |