Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,942,878	IS4 (+) +13 bp	intergenic (‑311/+28)	gcvA ← / ← ygdI	glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2942878 =	0 (0.000)	38 (0.700)	32/294	0.3	100%	intergenic (‑311/+40)	gcvA/ygdI	glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region
*	?	NC_000913	= 2942890	0 (0.000)	66 (1.220)	41/294	0.1	100%	intergenic (‑323/+28)	gcvA/ygdI	glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region

AAACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2943028‑2942878 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAG  >  NC_000913/4502090‑4502237                                                                                                                                                                                                                                                                                                             AAACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCG                                                                                                                                                      <  1:670777/151‑1   ACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTA                                                                                                                                                    >  2:790311/1‑151   ACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTA                                                                                                                                                    >  1:858142/1‑151   ACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTA                                                                                                                                                    >  2:2781/1‑151          ACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCC                                                                                                                                               <  1:615656/149‑1            TGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTC                                                                                                                                           <  2:38784/151‑1               TAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGT                                                                                                                                        >  1:262389/1‑151                  CGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAA                                                                                                                                     >  2:755709/1‑151                       CCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAAC                                                                                                                                <  1:517216/151‑1                        CGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACT                                                                                                                               <  1:92132/151‑1                        CGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAA                                                                                                                                 >  1:561193/1‑149                          GTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACGGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGTCGTAATGCCAGTCAGTTAAGCAACTG                                                                                                                              <  1:798931/150‑1                                         AAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGG                                                                                                              <  2:254467/151‑1                                                     GCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTA                                                                                                  >  1:849699/1‑151                                                        ACAAAAAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGACCGCAAGGAAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTCTCGGGGCTGCGGGGCATTT                                                                                               >  1:324829/1‑151                                                         CAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTC                                                                                              <  2:545134/151‑1                                                                           TACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACC                                                                            <  1:27843/151‑1                                                                           TACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACC                                                                            <  2:6708/151‑1                                                                           TACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTAC                                                                             <  1:586864/150‑1                                                                                ACGTGGAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTC                                                                        <  1:837023/150‑1                                                                                ACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTC                                                                        <  1:63689/150‑1                                                                                  GTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCG                                                                     >  2:544195/1‑151                                                                                     AAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGA                                                                  <  1:142541/151‑1                                                                                     AAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGA                                                                  >  1:461634/1‑151                                                                                          GATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGC                                                              >  1:145685/1‑150                                                                                                  AACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCC                                                      <  1:339957/150‑1                                                                                                    CTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTC                                                   <  2:762886/151‑1                                                                                                     TGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTGCCCTTCT                                                  <  1:841342/151‑1                                                                                                      GGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTT                                                 >  1:330601/1‑151                                                                                                        ATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGT                                               <  1:27305/151‑1                                                                                                                       TCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAG                                <  2:42718/151‑1                                                                                                                       TCGCAAGGTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAG                                <  1:375038/151‑1                                                                                                                              GTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCC                          >  2:525926/1‑150                                                                                                                              GTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCC                          >  2:610844/1‑150                                                                                                                              GTAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCC                          <  2:677459/150‑1                                                                                                                               TAAAAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCA                        <  1:30420/151‑1                                                                                                                                  AAAAAAGCACCGCAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATAC                     <  1:622470/151‑1                                                                                                                                              CAATTAGGCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCG          >  2:809118/1‑150                                                                                                                                                     GCGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAG  >  2:197441/1‑151                                                                                                                                                                                                                                                                                                             AAACCACAGACTGATAACGATACCGGTATGATTTCGTATAAAGACGCTAATGGCAACAAACAGCAGATCAACCGTACTGACGTGAAAGAGATGGTCGAACTGGATCAGTAATTCGCGATCGCAAGGTAAAAAAAAGCACCGCAATTAGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2943028‑2942878 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAG  >  NC_000913/4502090‑4502237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.