breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE512,6430AG72.4% 139.2 / 67.1 87intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
*USA300TCH1516_ALE602,4660GT28.6% 45.2 / 11.3 28intergenic (+56/‑104)hchA/araBMolecular chaperone Hsp31 and glyoxalase 3/Ribulokinase
*USA300TCH1516_ALE2,124,5280AG26.6% 127.0 / 24.3 64D219G (GAC→GGC) ‡lexA_2LexA repressor
*USA300TCH1516_ALE2,124,5290CA26.6% 151.0 / 18.8 64D219E (GAC→GAA) ‡lexA_2LexA repressor
*USA300TCH1516_ALE129,7081.T25.0% 33.4 / 12.0 16intergenic (‑121/+300)spa/sarSImmunoglobulin G‑binding protein A/HTH‑type transcriptional regulator SarS
*USA300TCH1516_ALE2,124,5130TG22.4% 149.5 / 18.4 68L214R (CTA→CGA) lexA_2LexA repressor
*USA300TCH1516_ALE2,124,5170TC22.4% 161.4 / 19.8 68N215N (AAT→AAClexA_2LexA repressor
*USA300TCH1516_ALE2,124,5270GC21.9% 165.7 / 23.8 64D219H (GAC→CAC) ‡lexA_2LexA repressor
*USA300TCH1516_ALE2,124,5230TC20.9% 152.1 / 19.2 68D217D (GAT→GAClexA_2LexA repressor
*USA300TCH1516_ALE2,124,5030CT20.0% 184.8 / 25.7 80L211L (CTA→TTA) lexA_2LexA repressor

Marginal new junction evidence (lowest skew 10 of 69 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000731 4294 =3 (1.100)14 (0.040) 9/282 0.1 2.1% intergenic (+259/‑206) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 2073 = 1294 (1.730)intergenic (+306/+20) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 = 16101 (0.370)14 (0.040) 10/278 0.1 4.1% intergenic (+395/‑461) repA/USA300HOU_pUSA300HOUMR0003 replication protein A/hypothetical protein
?NC_012417 = 1047 651 (0.880)coding (148/192 nt) USA300HOU_RS15665 hypothetical protein
* ? CP000731 = 43813 (1.100)5 (0.010) 5/284 0.2 0.97% intergenic (+346/‑119) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 = 1975 1016 (1.350)intergenic (+208/+118) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 4339 =4 (1.460)12 (0.030) 6/286 0.2 1.9% intergenic (+304/‑161) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 2021 = 1229 (1.620)intergenic (+254/+72) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 2687481120 (1.220)6 (0.070) 6/276 2.0 5.0% intergenic (+35/+34) clpL/USA300HOU_RS13835 ATP‑dependent Clp protease ATP‑binding subunit ClpL/hypothetical protein
?USA300TCH1516_ALE = 2687499 117 (1.290)intergenic (+53/+16) clpL/USA300HOU_RS13835 ATP‑dependent Clp protease ATP‑binding subunit ClpL/hypothetical protein
* ? USA300TCH1516_ALE = 2508448112 (1.140)5 (0.060) 5/276 2.1 4.4% intergenic (+36/+71) USA300TCH1516_02393/narT Acid shock protein/putative nitrate transporter NarT
?USA300TCH1516_ALE = 2508474 115 (1.270)intergenic (+62/+45) USA300TCH1516_02393/narT Acid shock protein/putative nitrate transporter NarT
* ? USA300TCH1516_ALE = 588673206 (2.100)5 (0.050) 5/282 2.2 2.5% coding (2577/3552 nt) rpoB DNA‑directed RNA polymerase subunit beta
?USA300TCH1516_ALE = 588680 199 (2.140)coding (2584/3552 nt) rpoB DNA‑directed RNA polymerase subunit beta
* ? USA300TCH1516_ALE 82395 =204 (2.080)10 (0.100) 5/290 2.2 4.9% coding (500/957 nt) nikB_1 Nickel transport system permease protein NikB
?USA300TCH1516_ALE 82432 = 193 (2.020)coding (537/957 nt) nikB_1 Nickel transport system permease protein NikB
* ? USA300TCH1516_ALE 2578084 =85 (0.870)4 (0.040) 4/280 2.3 5.3% coding (465/600 nt) USA300HOU_RS13285 hypothetical protein
?USA300TCH1516_ALE 2578167 = 62 (0.670)coding (382/600 nt) USA300HOU_RS13285 hypothetical protein
* ? USA300TCH1516_ALE 2378283 =95 (0.970)4 (0.040) 4/282 2.3 4.2% intergenic (+142/+277) glcU_2/USA300HOU_RS12220 putative glucose uptake protein GlcU/hypothetical protein
?USA300TCH1516_ALE 2378322 = 92 (0.990)intergenic (+181/+238) glcU_2/USA300HOU_RS12220 putative glucose uptake protein GlcU/hypothetical protein