breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
MC CP000731 1 Δ27,041 bp repAUSA300HOU_pUSA300HOUMR0033 26 genes
RA USA300TCH1516_ALE 478,502 C→A D342E (GAC→GAA nuoL → NADH‑quinone oxidoreductase subunit L
RA USA300TCH1516_ALE 584,121 G→T intergenic (+205/‑67) rplA → / → rplJ 50S ribosomal protein L1/50S ribosomal protein L10
RA USA300TCH1516_ALE 707,909 A→T M1M (TTG→ATG) † znuC_1 ← High‑affinity zinc uptake system ATP‑binding protein ZnuC
RA USA300TCH1516_ALE 973,213 C→A E175* (GAG→TAG)  yitU_1 ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU
RA USA300TCH1516_ALE 1,002,200 T→G M173R (ATG→AGG)  mecA → Adapter protein MecA 1
RA USA300TCH1516_ALE 1,105,994 T→C T12T (ACT→ACC pdhD → Dihydrolipoyl dehydrogenase
RA USA300TCH1516_ALE 1,196,508 C→A S338Y (TCT→TAT)  ftsZ → Cell division protein FtsZ
RA USA300TCH1516_ALE 1,232,053 A→T D233V (GAT→GTT)  stp → Serine/threonine phosphatase stp
RA USA300TCH1516_ALE 1,416,458 A→T I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,513,930 A→T L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,646,189 G→C A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,473 C→T A67T (GCT→ACT)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 2,010,363 C→T C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,289,257 A→C V78G (GTA→GGA)  cdaA ← Cyclic di‑AMP synthase CdaA
RA USA300TCH1516_ALE 2,337,959 C→T intergenic (‑125/‑283) adhR ← / → USA300HOU_RS11940 HTH‑type transcriptional regulator AdhR/Hyaluronate lyase
RA USA300TCH1516_ALE 2,455,692 Δ1 bp coding (1210/1260 nt) lyrA → Lysostaphin resistance protein A
RA USA300TCH1516_ALE 2,455,698 2 bp→AT coding (1216‑1217/1260 nt) lyrA → Lysostaphin resistance protein A
RA USA300TCH1516_ALE 2,532,298 A→T T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ USA300TCH1516_ALE 58811 58986 176 2 [1] [1] 2 USA300TCH1516_00049/USA300HOU_RS00245 hypothetical protein/hypothetical protein
* * ÷ USA300TCH1516_ALE 1171580 1171700 121 2 [1] [1] 2 hly/USA300HOU_RS05830 Alpha‑hemolysin/hypothetical protein
* * ÷ USA300TCH1516_ALE 1604567 1604652 86 2 [1] [1] 2 USA300HOU_RS07955/Int‑Tn_2 hypothetical protein/Transposase from transposon Tn916
* * ÷ USA300TCH1516_ALE 2055594 2055697 104 4 [1] [1] 2 purB/sspP Adenylosuccinate lyase/Staphopain A
* * ÷ USA300TCH1516_ALE 2507017 2507091 75 2 [1] [1] 2 USA300HOU_RS12900/sarZ hypothetical protein/HTH‑type transcriptional regulator SarZ

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 458102 =NA (NA)3 (0.030) 3/280 2.5 NA coding (712/1557 nt) hsdM_1 Type I restriction enzyme EcoKI M protein
?USA300TCH1516_ALE 458161 = NA (NA)coding (771/1557 nt) hsdM_1 Type I restriction enzyme EcoKI M protein
* ? USA300TCH1516_ALE 512620 =23 (0.230)47 (0.650)
+40 bp
41/218 0.3 73.6% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
* ? USA300TCH1516_ALE = 513613NA (NA)122 (2.010)
+57 bp
57/184 0.1 NA intergenic (+1681/‑5082) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 513672 = NA (NA)intergenic (+1740/‑5023) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
* ? USA300TCH1516_ALE 514102 =NA (NA)6 (0.060) 5/284 2.2 NA intergenic (+2170/‑4593) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 514124 = NA (NA)intergenic (+2192/‑4571) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
* ? USA300TCH1516_ALE = 514218NA (NA)4 (0.040) 4/288 2.3 NA intergenic (+2286/‑4477) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE = 514270 NA (NA)intergenic (+2338/‑4425) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
* ? USA300TCH1516_ALE = 515946NA (NA)3 (0.030) 3/284 2.5 NA intergenic (+4014/‑2749) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE = 515981 NA (NA)intergenic (+4049/‑2714) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
* ? USA300TCH1516_ALE = 517446NA (NA)16 (0.170) 9/284 1.7 NA intergenic (+5514/‑1249) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE = 517450 NA (NA)intergenic (+5518/‑1245) recR/speA_2 Recombination protein RecR/Arginine decarboxylase