breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,503,354 C→T pseudogene (296/432 nt) insP ← pseudogene, IS609 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,529,218 C→T pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
MC JC 2,393,422 Δ13 bp coding (1613‑1625/1842 nt) nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 3,442,845 G→A A418V (GCT→GTT)  secY ← preprotein translocase membrane subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,812 G→T intergenic (‑44/+22) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,296,384 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_pgi 365928 366385 458 3 [2] [2] 3 [lacZ] [lacZ]
* * ÷ NC_000913_3_vch_pgi 3423762–3424234 3424522–3424240 7–761 3 [2] [2] 3 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 97574 =5 (0.330)3 (0.210) 3/276 1.8 38.1% coding (488/1317 nt) murD UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
?NC_000913_3_vch_pgi 97595 = 5 (0.350)coding (509/1317 nt) murD UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
* ? NC_000913_3_vch_pgi = 129896210 (0.670)3 (0.200) 3/284 1.8 23.3% coding (365/648 nt) ychE UPF0056 family inner membrane protein
?NC_000913_3_vch_pgi = 1298992 10 (0.680)coding (395/648 nt) ychE UPF0056 family inner membrane protein