Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I0 R1 213 60.4 2554362 98.6% 2518600 147.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947_CJ‑RC 4,714,982 C→G 43.1% R36G (CGC→GGC)  PP_4173 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,714,9820CG43.1% 26.5 / 83.7 56R36G (CGC→GGC) PP_4173two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (19/12);  new base G (17/7);  total (36/20)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCCGCCGTCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGC  >  NC_002947_CJ‑RC/4714836‑4715123
                                                                                                                                                  |                                                                                                                                             
aaCGCCGCCGTCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACg                                                                                                                                              >  1:28188/1‑148 (MQ=255)
    ccgccgTCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATc                                                                                                                                          >  6:41135/1‑148 (MQ=255)
         gTCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGAc                                                                                                                                    <  4:127579/149‑1 (MQ=255)
          tCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGAcc                                                                                                                                   >  3:69930/1‑149 (MQ=255)
                 ccTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTgcgc                                                                                                                              >  3:47936/1‑147 (MQ=255)
                     gCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAgg                                                                                                                          <  1:107989/147‑1 (MQ=255)
                     gCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGaa                                                                                                                        <  1:119907/149‑1 (MQ=255)
                          gtgagaACCCAACCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACTCATATCATCAGTCTCGGCCCCTCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCaa                                                                                                                   <  3:134631/147‑1 (MQ=255)
                                        cATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAAc                                                                                                     >  8:218974/1‑149 (MQ=255)
                                            aGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGAt                                                                                                 <  5:54401/149‑1 (MQ=255)
                                             gCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATc                                                                                                <  4:233208/149‑1 (MQ=255)
                                              cAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCg                                                                                               >  1:288567/1‑149 (MQ=255)
                                                 aCGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCa                                                                                            <  1:754/149‑1 (MQ=255)
                                                     ttGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAc                                                                                                                                               >  4:134578/1‑94 (MQ=255)
                                                     ttGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAc                                                                                                                                               <  3:134578/94‑1 (MQ=255)
                                                        aGCTGGGACATTCATACCCTCACGCAGATCATCAGTCTCGGCCACGCCCTGCTACTGACCTTGCTCTTGATCATCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGtt                                                                                     <  2:214516/149‑1 (MQ=255)
                                                        aGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGtt                                                                                     <  2:150928/149‑1 (MQ=255)
                                                           tGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTgg                                                                                   >  1:74018/1‑148 (MQ=255)
                                                                 aTTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGc                                                                            <  3:209507/149‑1 (MQ=255)
                                                                  ttCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCg                                                                           >  3:78293/1‑149 (MQ=255)
                                                                  ttCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCg                                                                           >  6:81128/1‑149 (MQ=255)
                                                                  ttCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCg                                                                           >  2:144486/1‑149 (MQ=255)
                                                                     aTACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCg                                                                        <  4:196071/149‑1 (MQ=255)
                                                                     aTACCCGCACGCAGATCATCAGACTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTc                                                                         <  7:87175/148‑1 (MQ=255)
                                                                         ccGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGaa                                                                     >  2:179923/1‑148 (MQ=255)
                                                                         ccGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGaa                                                                     >  2:122819/1‑148 (MQ=255)
                                                                         ccGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGaa                                                                     >  1:80449/1‑148 (MQ=255)
                                                                         ccGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGaa                                                                     >  7:221628/1‑148 (MQ=255)
                                                                           gcacgcaGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGACGGAatat                                                                  >  4:246712/1‑149 (MQ=255)
                                                                              cgcaGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATggg                                                               <  2:253658/149‑1 (MQ=255)
                                                                               gcaGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACATTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATgggg                                                              >  5:215558/1‑149 (MQ=255)
                                                                                 aGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTg                                                            >  3:250703/1‑149 (MQ=255)
                                                                                      atcaGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGAtctc                                                       <  8:126645/149‑1 (MQ=255)
                                                                                      atcaGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGAtctc                                                       >  8:284340/1‑149 (MQ=255)
                                                                                         aGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTATGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCgg                                                     <  8:263082/148‑1 (MQ=255)
                                                                                             tcGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGcaa                                                 >  3:206801/1‑148 (MQ=255)
                                                                                                  cccGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGa                                           <  5:81128/149‑1 (MQ=255)
                                                                                                        cTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCt                                     >  8:82971/1‑149 (MQ=255)
                                                                                                        cTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCt                                     >  4:280910/1‑149 (MQ=255)
                                                                                                           cTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCtt                                    <  2:28188/147‑1 (MQ=255)
                                                                                                              cTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAg                                <  2:233509/148‑1 (MQ=255)
                                                                                                                gACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGc                             >  8:238032/1‑149 (MQ=255)
                                                                                                                 aCCTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAggg                              <  5:82794/147‑1 (MQ=255)
                                                                                                                 aCCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGc                             <  4:69930/148‑1 (MQ=255)
                                                                                                                   cTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGa                          >  8:296498/1‑149 (MQ=255)
                                                                                                                   cTTGCTCTTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGa                          >  3:45198/1‑149 (MQ=255)
                                                                                                                   cTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGa                          >  5:90310/1‑149 (MQ=255)
                                                                                                                   cTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGa                          >  1:163239/1‑149 (MQ=255)
                                                                                                                        tctTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGt                     >  8:62929/1‑149 (MQ=255)
                                                                                                                         ctTGATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTg                    >  2:316029/1‑149 (MQ=255)
                                                                                                                            gATCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCa                 >  4:58119/1‑149 (MQ=255)
                                                                                                                              tCAGCTTCTTTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACAAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCAcc               >  1:53203/1‑149 (MQ=255)
                                                                                                                                   ttcttTACCTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAg          >  1:3875/1‑149 (MQ=255)
                                                                                                                                   ttcttTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAg          >  2:166664/1‑149 (MQ=255)
                                                                                                                                   ttcttTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAg          >  1:312718/1‑149 (MQ=255)
                                                                                                                                       ttACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATc      >  3:251588/1‑149 (MQ=255)
                                                                                                                                           cTTCGTAGGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAATTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCg   >  1:57120/1‑148 (MQ=255)
                                                                                                                                           cTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATTTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGc  >  6:251343/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
AACGCCGCCGTCAAGGCCCTGGCCGAGGGAGAACCCACCCCATGAGCAAACGCTTGAGCTGGGACATTCATACCCGCACGCAGATCATCAGTCTCGGCCCCGCCCTGCTACTGACCTTGCTCTTGATCAGCTTCTTTACCTTCGTACGCATCCAGGACCTGCGCCAGGAACTCAACCACACCGGGCAACTGATCGCCAACCAGTTGGCACCGGCGTCGGAATATGGGGTGATCTCGGGCAACAACGAAGTGCTTGAAGGGCTGATGCGTGCCACCCTCAGCATCCCGC  >  NC_002947_CJ‑RC/4714836‑4715123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A