Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F64 I1 R1
|
60 |
70.4 |
2989830 |
98.5% |
2944982 |
146.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,586,164 |
+C |
intergenic (+140/+75) |
PP_4061 → / ← PP_4063 |
hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,586,163 | 1 | . | C | 95.8%
| 79.6
/ ‑2.9
| 24 | intergenic (+139/+76) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (9/14); total (10/14) |
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
GGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC > NC_002947_CJ‑RC/4586018‑4586306
|
ggCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGA‑cc < 4:159102/149‑2 (MQ=255)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCtt < 7:151296/148‑1 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCtt < 6:87904/148‑1 (MQ=39)
gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCtt < 4:98813/148‑1 (MQ=39)
ccACGGCACCGGCGTGAACAGCTTATCTGCGCTGATCCATTGAAAGTTTGAGGATGACCGGGTGCTTTGCAAGTTGAAGATCTTGCATTGATCGGGCCTGCCTCTTCGC‑GTGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGa < 5:10460/148‑1 (MQ=255)
aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATg < 3:138156/149‑1 (MQ=39)
aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTg < 2:299606/148‑1 (MQ=39)
aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTa < 1:169729/149‑1 (MQ=39)
aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTa < 5:93977/149‑1 (MQ=39)
aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGa < 2:333196/147‑1 (MQ=39)
cgcgGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGtt < 1:122808/149‑1 (MQ=38)
cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGc < 7:380244/144‑1 (MQ=38)
cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCggg < 3:30001/147‑1 (MQ=38)
cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCgg < 7:276777/146‑1 (MQ=38)
ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 4:163924/147‑1 (MQ=38)
gg‑gTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCCGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACCCTGATCTCCCGCATg > 6:161386/2‑149 (MQ=38)
g‑gTAAACCCGCTCCTACCAGGGG‑CAGCGCC‑GCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGc > 5:298231/1‑149 (MQ=18)
g‑gTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGc > 3:238626/1‑149 (MQ=255)
tAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCg > 5:34619/1‑148 (MQ=255)
aaaCCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCgg > 3:32329/1‑148 (MQ=255)
cccGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt > 7:147571/1‑149 (MQ=255)
cccGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACt > 3:199982/1‑149 (MQ=255)
cTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGTCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAc > 6:54044/1‑149 (MQ=255)
cTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAc > 4:35561/1‑149 (MQ=255)
aGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGc > 4:124519/1‑149 (MQ=255)
|
GGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC > NC_002947_CJ‑RC/4586018‑4586306
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTT > NC_002947_CJ‑RC/4586029‑4586295
|
AGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTG < NB501016:282:H2V7GAFX2:4:11605:21098:5378/150‑1 (MQ=60)
ACCACGGCACCGGCGTGAACAGCTTATCTGCGCTGATCCATTGAAAGTTTGAGGATGACCGGGTGCTTTGCAAGTTGAAGATCTTGCATTGATCGGGCCTGCCTCTTCGC‑GTGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA < NB501016:282:H2V7GAFX2:3:11403:25329:1361/150‑1 (MQ=60)
CACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGC < NB501016:282:H2V7GAFX2:2:11303:13010:12625/151‑1 (MQ=60)
CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG < NB501016:282:H2V7GAFX2:1:11308:25376:10014/151‑1 (MQ=60)
CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG < NB501016:282:H2V7GAFX2:3:11507:16744:5172/151‑1 (MQ=60)
GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT < NB501016:282:H2V7GAFX2:2:11106:25812:18205/149‑1 (MQ=60)
GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGG < NB501016:282:H2V7GAFX2:4:21504:4805:14137/148‑1 (MQ=60)
GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG < NB501016:282:H2V7GAFX2:4:21612:10586:6072/146‑1 (MQ=60)
GCGCGGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGG‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTT < NB501016:282:H2V7GAFX2:1:11211:13162:6393/151‑1 (MQ=60)
G‑G‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCG > NB501016:282:H2V7GAFX2:2:21111:14664:2324/1‑151 (MQ=60)
G‑GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC < NB501016:282:H2V7GAFX2:3:21510:13057:10567/73‑1 (MQ=60)
GTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG > NB501016:282:H2V7GAFX2:3:11407:12962:16341/1‑150 (MQ=60)
TAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGA > NB501016:282:H2V7GAFX2:2:11107:23947:7679/1‑150 (MQ=60)
ACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTT > NB501016:282:H2V7GAFX2:2:21103:23075:3276/1‑151 (MQ=60)
ACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTT > NB501016:282:H2V7GAFX2:4:11604:16274:10475/1‑151 (MQ=60)
|
AGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑G‑GTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTT > NC_002947_CJ‑RC/4586029‑4586295
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
