Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I0 R1 213 60.4 2554362 98.6% 2518600 147.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC  >  NC_002947_CJ‑RC/4586027‑4586306
                                                                                                                                            |                                                                                                                                              
GTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTT                                                                                                                                     <  NB501016:282:H2V7GAFX2:3:21506:12470:15502/151‑1 (MQ=60)
   GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAA                                                                                                                                  <  NB501016:282:H2V7GAFX2:3:21602:12588:17995/151‑1 (MQ=60)
   GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGC                                                                                                                                        <  NB501016:282:H2V7GAFX2:1:21306:14497:14022/145‑1 (MQ=60)
       ACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCG                                                                                                                              <  NB501016:282:H2V7GAFX2:1:21201:7519:13881/151‑1 (MQ=60)
            ACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCT                                                                                                                         <  NB501016:282:H2V7GAFX2:2:11210:15801:19606/151‑1 (MQ=60)
                      ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                                                                                                               <  NB501016:282:H2V7GAFX2:2:11104:19931:13009/151‑1 (MQ=60)
                                 GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT                                                                                                      <  NB501016:282:H2V7GAFX2:1:21208:12033:5187/149‑1 (MQ=60)
                                 GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCG                                                                                                         <  NB501016:282:H2V7GAFX2:3:21504:21999:14606/146‑1 (MQ=60)
                                         CCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAAT                                                                                                                                 <  NB501016:282:H2V7GAFX2:2:11209:18608:17209/114‑1 (MQ=60)
                                                                                    ATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                                >  NB501016:282:H2V7GAFX2:4:21410:20666:6553/1‑88 (MQ=51)
                                                                                                                 cgGGTAAACCCGCTCCTACCAGGGCC‑CGTGCCGGCCTTGAAAGCGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACGGATCTCACGCATGC                    >  NB501016:282:H2V7GAFX2:2:21112:12291:13168/3‑151 (MQ=60)
                                                                                                                             CTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGC         >  NB501016:282:H2V7GAFX2:2:11303:10029:13660/1‑150 (MQ=60)
                                                                                                                                   CCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC  <  NB501016:282:H2V7GAFX2:1:21107:13533:16555/151‑1 (MQ=60)
                                                                                                                                   CCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC  >  NB501016:282:H2V7GAFX2:4:11407:2624:2645/1‑151 (MQ=60)
                                                                                                                                   CCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC  >  NB501016:282:H2V7GAFX2:4:21403:6898:5491/1‑151 (MQ=60)
                                                                                                                                   CCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGAACATCAACCCGCAGATGCAGCCGCAATCTACGCCACACTGATCTCACGCATGCGGAACTTCTGCACATTGC  >  NB501016:282:H2V7GAFX2:3:21604:12556:18491/1‑151 (MQ=60)
                                                                                                                                            |                                                                                                                                              
GTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGC  >  NC_002947_CJ‑RC/4586027‑4586306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑