Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F61 I1 R1 63 66.6 2847048 98.6% 2807189 146.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGA  >  NC_002947_CJ‑RC/699572‑699863
                                                                                                                                                      |                                                                                                                                             
AGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGG                                                                                                                                               >  NB501016:282:H2V7GAFX2:3:11608:18682:10465/1‑151 (MQ=32)
 GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGAC                                                                                                                                              <  NB501016:282:H2V7GAFX2:4:21502:2630:7848/151‑1 (MQ=32)
  GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCT                                                                                                                                             <  NB501016:282:H2V7GAFX2:4:21501:1197:11797/151‑1 (MQ=32)
     GTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGA                                                                                                                                           >  NB501016:282:H2V7GAFX2:2:11310:18021:8757/1‑150 (MQ=32)
           GGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAG                                                                                                                                    <  NB501016:282:H2V7GAFX2:3:11509:6410:18555/151‑1 (MQ=31)
              TCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTT                                                                                                                                  <  NB501016:282:H2V7GAFX2:4:21604:8731:4014/150‑1 (MQ=32)
              TCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTT                                                                                                                                  <  NB501016:282:H2V7GAFX2:2:11104:25438:6427/150‑1 (MQ=32)
                 ATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAC                                                                                                                              <  NB501016:282:H2V7GAFX2:3:21603:20782:10131/151‑1 (MQ=31)
                 ATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAC                                                                                                                              <  NB501016:282:H2V7GAFX2:1:11310:22668:17447/151‑1 (MQ=31)
                     CGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTTAACACCAGTTTCACTGCT                                                                                                                          <  NB501016:282:H2V7GAFX2:1:21201:23096:7417/151‑1 (MQ=60)
                           GCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGA                                                                                                                    <  NB501016:282:H2V7GAFX2:3:11603:16115:13184/151‑1 (MQ=60)
                            CTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:3:11606:11446:9365/148‑1 (MQ=33)
                                ACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGG                                                                                                               <  NB501016:282:H2V7GAFX2:2:11104:17885:11655/151‑1 (MQ=31)
                                ACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGG                                                                                                               <  NB501016:282:H2V7GAFX2:4:21504:6096:4386/151‑1 (MQ=32)
                                 CCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGC                                                                                                              <  NB501016:282:H2V7GAFX2:1:21208:11674:4350/151‑1 (MQ=31)
                                 CCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGC                                                                                                              >  NB501016:282:H2V7GAFX2:3:21606:19935:16370/1‑151 (MQ=60)
                                      TTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAA                                                                                                           <  NB501016:282:H2V7GAFX2:2:21102:18652:14599/149‑1 (MQ=32)
                                      TTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAA                                                                                                           <  NB501016:282:H2V7GAFX2:4:11407:26213:4813/149‑1 (MQ=32)
                                      TTAGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAG                                                                                                          <  NB501016:282:H2V7GAFX2:3:11507:1480:17952/150‑1 (MQ=32)
                                                 CAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                              <  NB501016:282:H2V7GAFX2:2:21109:1938:4022/151‑1 (MQ=31)
                                                 CAGTTACTTGACAGAACTTAGAAATGAACATTCAGCTCGAATGTTGAATTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGAAATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                              <  NB501016:282:H2V7GAFX2:3:21512:26676:2028/151‑1 (MQ=30)
                                                  AGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGA                                                                                             <  NB501016:282:H2V7GAFX2:4:11511:8993:8315/151‑1 (MQ=30)
                                                      ACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAG                                                                                            <  NB501016:282:H2V7GAFX2:1:11206:24715:11525/148‑1 (MQ=21)
                                                       CTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGT                                                                                           >  NB501016:282:H2V7GAFX2:3:21607:2568:17022/1‑148 (MQ=22)
                                                          GTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCT                                                                                     >  NB501016:282:H2V7GAFX2:3:11402:2408:1653/1‑151 (MQ=22)
                                                           TCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTC                                                                                    >  NB501016:282:H2V7GAFX2:2:11204:11418:7213/1‑151 (MQ=22)
                                                            CAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCG                                                                                   <  NB501016:282:H2V7GAFX2:4:11507:23528:10492/151‑1 (MQ=21)
                                                               AACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAA                                                                                 <  NB501016:282:H2V7GAFX2:2:11109:25391:5611/150‑1 (MQ=22)
                                                                   TAGAAATTAACATTCATCTCTAATGTTGATTTCTGGCTTTTGTCAGATCGTTCATTAAAAATTCTGATATGTGATTGATATAGGCTGAACACGATTTTCACTTCTGGTGTTTCAGGCTAAGGTTAAATTTGTGAGTTCTGCTCGAAAGTG                                                                             <  NB501016:282:H2V7GAFX2:2:11304:7228:7526/150‑1 (MQ=2)
                                                                    AGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGC                                                                            >  NB501016:282:H2V7GAFX2:2:11210:6892:7630/1‑150 (MQ=22)
                                                                       AATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACG                                                                        >  NB501016:282:H2V7GAFX2:2:11307:8314:15557/1‑151 (MQ=21)
                                                                         TGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGT                                                                       >  NB501016:282:H2V7GAFX2:1:21208:15669:11943/1‑150 (MQ=21)
                                                                         TGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTG                                                                                                <  NB501016:282:H2V7GAFX2:3:11601:24784:12013/125‑1 (MQ=23)
                                                                          GAACATTCGGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTA                                                                      <  NB501016:282:H2V7GAFX2:3:11409:4109:16345/150‑1 (MQ=6)
                                                                          GAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAC                                                                     >  NB501016:282:H2V7GAFX2:1:11205:11449:1109/1‑151 (MQ=22)
                                                                           AACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACG                                                                    >  NB501016:282:H2V7GAFX2:3:21402:24493:4106/1‑151 (MQ=22)
                                                                              ATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAT                                                                 >  NB501016:282:H2V7GAFX2:2:21311:13138:18137/1‑151 (MQ=8)
                                                                                   GCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG                                                            <  NB501016:282:H2V7GAFX2:1:21112:17071:4517/151‑1 (MQ=0)
                                                                                         ATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAAT                                                      <  NB501016:282:H2V7GAFX2:2:21210:2579:3241/151‑1 (MQ=0)
                                                                                            TTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGT                                                    <  NB501016:282:H2V7GAFX2:4:21601:5402:13674/150‑1 (MQ=0)
                                                                                                  TCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTT                                              >  NB501016:282:H2V7GAFX2:4:11401:11057:11325/1‑150 (MQ=0)
                                                                                                      GCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAG                                         <  NB501016:282:H2V7GAFX2:1:21204:26060:5523/151‑1 (MQ=0)
                                                                                                            GTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAAC                                   >  NB501016:282:H2V7GAFX2:2:21308:22642:17458/1‑151 (MQ=0)
                                                                                                              CAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCA                                 <  NB501016:282:H2V7GAFX2:2:11307:4144:17340/151‑1 (MQ=0)
                                                                                                               AGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAG                                <  NB501016:282:H2V7GAFX2:3:21611:6216:6450/151‑1 (MQ=0)
                                                                                                                         TTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTT                         <  NB501016:282:H2V7GAFX2:1:21309:12229:8338/148‑1 (MQ=0)
                                                                                                                          TAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGG                      <  NB501016:282:H2V7GAFX2:3:21509:11190:16300/150‑1 (MQ=0)
                                                                                                                           AAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGT                    >  NB501016:282:H2V7GAFX2:3:21602:16101:11675/1‑151 (MQ=0)
                                                                                                                             AAATTCGGATTTGTGATAGATATAGACTGAACAGGAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTA                  <  NB501016:282:H2V7GAFX2:1:21302:24569:18565/151‑1 (MQ=0)
                                                                                                                             AAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTA                  <  NB501016:282:H2V7GAFX2:2:11201:6231:8425/151‑1 (MQ=0)
                                                                                                                               ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTAT                 >  NB501016:282:H2V7GAFX2:3:11609:22065:15883/1‑150 (MQ=0)
                                                                                                                                TTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCT                                                                                     <  NB501016:282:H2V7GAFX2:2:11311:12279:7578/81‑1 (MQ=0)
                                                                                                                                 TCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATAT               <  NB501016:282:H2V7GAFX2:4:21408:24141:20016/150‑1 (MQ=0)
                                                                                                                                         GTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTG      <  NB501016:282:H2V7GAFX2:1:21102:17247:1345/151‑1 (MQ=0)
                                                                                                                                           GATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGA     >  NB501016:282:H2V7GAFX2:1:11311:14997:8579/1‑150 (MQ=0)
                                                                                                                                            ATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAG   <  NB501016:282:H2V7GAFX2:1:21209:6643:6546/151‑1 (MQ=0)
                                                                                                                                            ATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAG   <  NB501016:282:H2V7GAFX2:2:11210:3914:7305/151‑1 (MQ=0)
                                                                                                                                              AGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGA  <  NB501016:282:H2V7GAFX2:3:11604:15600:13274/150‑1 (MQ=0)
                                                                                                                                                      |                                                                                                                                             
AGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGA  >  NC_002947_CJ‑RC/699572‑699863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑