Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F25 I1 R1 61 74.4 3161000 98.6% 3116746 146.7

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGAAGC  >  NC_002947_CJ‑RC/699573‑699866
                                                                                                                                                     |                                                                                                                                                
GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGAC                                                                                                                                                 <  NB501016:282:H2V7GAFX2:4:11601:7425:6174/151‑1 (MQ=32)
GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTCGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGC                                                                                                                                                 <  NB501016:282:H2V7GAFX2:4:11508:25074:1929/151‑1 (MQ=31)
 GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACT                                                                                                                                                <  NB501016:282:H2V7GAFX2:3:21606:20627:13956/151‑1 (MQ=32)
      CAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAAC                                                                                                                                            <  NB501016:282:H2V7GAFX2:1:11104:5107:20327/150‑1 (MQ=32)
      CAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAAC                                                                                                                                            >  NB501016:282:H2V7GAFX2:3:21602:4090:13770/1‑150 (MQ=32)
        GCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACC                                                                                                                                         <  NB501016:282:H2V7GAFX2:4:11503:18610:18586/151‑1 (MQ=31)
          GGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAG                                                                                                                                       <  NB501016:282:H2V7GAFX2:2:11205:18458:3022/151‑1 (MQ=31)
              CGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACCTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTC                                                                                                                                   <  NB501016:282:H2V7GAFX2:3:11505:26139:6999/151‑1 (MQ=31)
              CGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTC                                                                                                                                   >  NB501016:282:H2V7GAFX2:2:11209:15455:2691/1‑151 (MQ=31)
              CGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTAAACACCAGTTTC                                                                                                                                   <  NB501016:282:H2V7GAFX2:3:21501:16837:12164/151‑1 (MQ=31)
               GATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTC                                                                                                                                   >  NB501016:282:H2V7GAFX2:3:21406:25424:16028/1‑150 (MQ=32)
                ATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCAC                                                                                                                                 >  NB501016:282:H2V7GAFX2:1:21204:16097:3526/1‑151 (MQ=31)
                  CCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTG                                                                                                                               <  NB501016:282:H2V7GAFX2:4:11605:14324:6777/151‑1 (MQ=31)
                      CTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGG                                                                                                                           >  NB501016:282:H2V7GAFX2:4:21604:2619:9996/1‑151 (MQ=32)
                        TGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGG                                                                                                                           <  NB501016:282:H2V7GAFX2:3:21602:19465:5492/149‑1 (MQ=60)
                         GGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTG                                                                                                                         <  NB501016:282:H2V7GAFX2:4:11401:6893:7414/150‑1 (MQ=32)
                           CTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGAT                                                                                                                      >  NB501016:282:H2V7GAFX2:3:21603:23171:20040/1‑151 (MQ=31)
                           CTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGAT                                                                                                                      >  NB501016:282:H2V7GAFX2:1:11312:1206:5629/1‑151 (MQ=31)
                              CACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACTTTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACAAGTTTCACTGCTGGTGGATC                                                                                                                     >  NB501016:282:H2V7GAFX2:4:11509:1871:4513/1‑149 (MQ=30)
                              CACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAG                                                                                                                   <  NB501016:282:H2V7GAFX2:1:11310:5322:4727/151‑1 (MQ=60)
                              CACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATC                                                                                                                     >  NB501016:282:H2V7GAFX2:3:21512:8660:2092/1‑149 (MQ=60)
                              CACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAG                                                                                                                   <  NB501016:282:H2V7GAFX2:2:11108:20219:19573/151‑1 (MQ=32)
                               ACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGG                                                                                                                  >  NB501016:282:H2V7GAFX2:4:11404:16542:16571/1‑151 (MQ=32)
                                CCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAG                                                                                                                   >  NB501016:282:H2V7GAFX2:3:21509:22447:6116/1‑149 (MQ=60)
                                CCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGC                                                                                                                 >  NB501016:282:H2V7GAFX2:4:11609:15225:15953/1‑151 (MQ=32)
                                  ACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTA                                                                                                               <  NB501016:282:H2V7GAFX2:1:11107:2546:5379/151‑1 (MQ=32)
                                   CTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTA                                                                                                               >  NB501016:282:H2V7GAFX2:1:11207:19029:16899/1‑150 (MQ=32)
                                   CTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTA                                                                                                               >  NB501016:282:H2V7GAFX2:2:21101:5401:20240/1‑150 (MQ=32)
                                     TTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAG                                                                                                             <  NB501016:282:H2V7GAFX2:4:11404:8233:20040/150‑1 (MQ=32)
                                      TCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGG                                                                                                            <  NB501016:282:H2V7GAFX2:4:11608:2351:16107/150‑1 (MQ=32)
                                           GTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAT                                                                                                      <  NB501016:282:H2V7GAFX2:3:11406:20199:2544/151‑1 (MQ=31)
                                              CGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATGTGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTG                                                                                                   >  NB501016:282:H2V7GAFX2:4:11506:23966:2955/1‑151 (MQ=60)
                                                CAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                                 <  NB501016:282:H2V7GAFX2:4:21501:9490:19531/151‑1 (MQ=32)
                                                CAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                                 <  NB501016:282:H2V7GAFX2:1:21202:23634:15567/151‑1 (MQ=32)
                                                  GTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAG                                                                                               <  NB501016:282:H2V7GAFX2:4:11603:10134:14662/151‑1 (MQ=27)
                                                  GTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                                 >  NB501016:282:H2V7GAFX2:1:21102:18053:13673/1‑149 (MQ=28)
                                                  GTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTG                                                                                                 >  NB501016:282:H2V7GAFX2:4:21403:7979:4037/1‑149 (MQ=28)
                                                      CTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAG                                                                                                                   <  NB501016:282:H2V7GAFX2:1:21211:3864:4198/127‑1 (MQ=23)
                                                         GTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCT                                                                                        <  NB501016:282:H2V7GAFX2:1:21101:4633:1829/151‑1 (MQ=21)
                                                         GTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGC                                                                                         <  NB501016:282:H2V7GAFX2:3:11406:15478:11409/150‑1 (MQ=22)
                                                         GTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCAC                                                                                                                                 <  NB501016:282:H2V7GAFX2:2:11109:25992:9904/110‑1 (MQ=25)
                                                          TCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTC                                                                                       >  NB501016:282:H2V7GAFX2:4:21502:23564:6902/1‑151 (MQ=21)
                                                           CAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTC                                                                                       <  NB501016:282:H2V7GAFX2:4:11510:12367:19945/150‑1 (MQ=22)
                                                           CAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGATCTGCTCG                                                                                      >  NB501016:282:H2V7GAFX2:2:11306:20507:9444/1‑151 (MQ=21)
                                                             GAACTTAGAAATTAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGTATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAA                                                                                    <  NB501016:282:H2V7GAFX2:3:21510:3384:20284/151‑1 (MQ=20)
                                                             GAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATC                                                                                                                     <  NB501016:282:H2V7GAFX2:4:11511:22675:15543/118‑1 (MQ=53)
                                                               ACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAG                                                                                  <  NB501016:282:H2V7GAFX2:3:11610:23416:6884/151‑1 (MQ=22)
                                                                CTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAA                                                                                    >  NB501016:282:H2V7GAFX2:1:21202:2093:6745/1‑148 (MQ=21)
                                                                CTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAA                                                                                    >  NB501016:282:H2V7GAFX2:4:11609:15360:3789/1‑148 (MQ=22)
                                                                   AGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGC                                                                               <  NB501016:282:H2V7GAFX2:4:21605:12692:12600/150‑1 (MQ=21)
                                                                      AATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACG                                                                           >  NB501016:282:H2V7GAFX2:2:11209:10513:17420/1‑151 (MQ=21)
                                                                        TGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGT                                                                          >  NB501016:282:H2V7GAFX2:4:11406:25591:12425/1‑150 (MQ=21)
                                                                         GAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAC                                                                        >  NB501016:282:H2V7GAFX2:1:11202:16217:5184/1‑151 (MQ=21)
                                                                         GAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAC                                                                        <  NB501016:282:H2V7GAFX2:4:21608:8961:17871/151‑1 (MQ=22)
                                                                          AACATTCAGCTCGTATGGTGATTTCTGGCTTTTTTCATATCGTTCTTTAAAAATTCGGTTATTTGTTAGATATACGCGTAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACG                                                                       <  NB501016:282:H2V7GAFX2:3:11403:26214:15025/151‑1 (MQ=13)
                                                                          AACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAC                                                                        <  NB501016:282:H2V7GAFX2:1:21101:25919:9978/150‑1 (MQ=22)
                                                                            CATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGA                                                                      >  NB501016:282:H2V7GAFX2:4:11401:21917:19077/1‑150 (MQ=17)
                                                                              TTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAAGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGATAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATT                                                                   >  NB501016:282:H2V7GAFX2:4:21402:2415:12356/1‑151 (MQ=5)
                                                                                 AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTC                                                                <  NB501016:282:H2V7GAFX2:4:21505:26439:19250/151‑1 (MQ=0)
                                                                                 AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTC                                                                <  NB501016:282:H2V7GAFX2:1:11312:17299:13895/151‑1 (MQ=0)
                                                                                  GCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG                                                               <  NB501016:282:H2V7GAFX2:4:11401:18889:16614/151‑1 (MQ=0)
                                                                                  GCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG                                                               <  NB501016:282:H2V7GAFX2:2:11106:16777:20318/151‑1 (MQ=0)
                                                                                     CGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCG                                                            <  NB501016:282:H2V7GAFX2:3:11605:1659:10251/151‑1 (MQ=0)
                                                                                          GTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATG                                                        <  NB501016:282:H2V7GAFX2:3:11612:11895:1643/150‑1 (MQ=0)
                                                                                          GTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGT                                                       >  NB501016:282:H2V7GAFX2:3:11501:5625:8452/1‑151 (MQ=0)
                                                                                            TGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCG                                                     >  NB501016:282:H2V7GAFX2:4:21507:20083:12935/1‑151 (MQ=0)
                                                                                                TTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCT                                                  <  NB501016:282:H2V7GAFX2:3:21407:13953:13374/150‑1 (MQ=0)
                                                                                                TTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTT                                                 >  NB501016:282:H2V7GAFX2:2:11104:15755:10043/1‑151 (MQ=0)
                                                                                                  CTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTC                                                >  NB501016:282:H2V7GAFX2:3:21503:9922:6652/1‑150 (MQ=0)
                                                                                                    TGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCAC                                              <  NB501016:282:H2V7GAFX2:3:11503:22138:2450/150‑1 (MQ=0)
                                                                                                    GGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTC                                                <  NB501016:282:H2V7GAFX2:1:11312:22641:20289/148‑1 (MQ=0)
                                                                                                    GGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCAC                                              >  NB501016:282:H2V7GAFX2:3:11402:15039:9531/1‑150 (MQ=0)
                                                                                                             CAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATA                                        <  NB501016:282:H2V7GAFX2:4:21403:23385:8040/147‑1 (MQ=0)
                                                                                                              AGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAG                                   <  NB501016:282:H2V7GAFX2:4:21403:1570:4175/151‑1 (MQ=0)
                                                                                                              AGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACC                                     >  NB501016:282:H2V7GAFX2:2:21204:23155:11264/1‑149 (MQ=0)
                                                                                                              AGATCGTTCTTTAAAAATTCGGATATGTGATAGATAAAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACC                                     >  NB501016:282:H2V7GAFX2:3:21403:10711:12211/1‑149 (MQ=0)
                                                                                                                       TTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGG                          >  NB501016:282:H2V7GAFX2:1:21207:21268:2056/1‑151 (MQ=0)
                                                                                                                         TAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGG                         <  NB501016:282:H2V7GAFX2:4:11406:24615:13569/150‑1 (MQ=0)
                                                                                                                                 CGGTTATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATG                 <  NB501016:282:H2V7GAFX2:1:11208:22876:17319/150‑1 (MQ=0)
                                                                                                                                              GATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGA     >  NB501016:282:H2V7GAFX2:4:11502:15194:4061/1‑149 (MQ=0)
                                                                                                                                               ATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGAAGC  <  NB501016:282:H2V7GAFX2:3:21511:22595:13423/151‑1 (MQ=0)
                                                                                                                                               ATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGAAG   <  NB501016:282:H2V7GAFX2:2:21208:22272:19267/150‑1 (MQ=0)
                                                                                                                                                     |                                                                                                                                                
GGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTCAGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCGGCGAATGTCGTCTTCACAGTATAACCAGATTGCTTGGGGTTATATGGTCAAGTGAAGAAGC  >  NC_002947_CJ‑RC/699573‑699866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑