Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F85 I1 R1	58	55.8	2477618	98.2%	2433020	140.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947_CJ‑RC	5,396,064	A→G	V231A (GTA→GCA)	tphBII ←	4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947_CJ‑RC	5,396,064	0	A	G	100.0%	120.6 / NA	34	V231A (GTA→GCA)	tphBII	4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/20); total (14/20)

CCTGCCACCGATAGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTACTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGC  >  NC_002947_CJ‑RC/5395922‑5396202

ccTGCCACCGATAGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGc                                                                                                                                      >  6:194656/1‑149 (MQ=255)

             gatagTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCa                                                                                                                          <  3:229322/148‑1 (MQ=255)

              atagTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAgt                                                                                                                        <  5:194656/149‑1 (MQ=255)

                agTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCACGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAgtgt                                                                                                                      >  3:96127/1‑149 (MQ=255)

                 gTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAgtgt                                                                                                                      <  1:135600/148‑1 (MQ=255)

                    cgcTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGtctc                                                                                                                   <  2:239397/148‑1 (MQ=255)

                           tCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCgg                                                                                                           >  5:222802/1‑149 (MQ=255)

                              gTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGaa                                                                                                        <  4:240422/149‑1 (MQ=255)

                              gTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGaa                                                                                                        <  6:222802/149‑1 (MQ=255)

                                  ggTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGt                                                                                                    <  2:140754/149‑1 (MQ=255)

                                      ccAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATc                                                                                                <  7:250380/149‑1 (MQ=255)

                                       cagcagCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCt                                                                                               >  3:272634/1‑149 (MQ=255)

                                         gcagcTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTgg                                                                                             >  7:220583/1‑149 (MQ=255)

                                         gcagcTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTgg                                                                                             <  3:230144/149‑1 (MQ=255)

                                                          tGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGaa                                                                            >  8:75258/1‑149 (MQ=255)

                                                             cccTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGaaca                                                                          >  1:76247/1‑148 (MQ=255)

                                                                     gTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCtt                                                                 <  8:220583/149‑1 (MQ=255)

                                                                       gcagcaTGGCTACATACAGGTCGTTCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCg                                                                                                            <  8:174487/104‑1 (MQ=255)

                                                                       gcagcaTGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTATCTCGACAGCg                                                                                                            >  7:174487/1‑104 (MQ=255)

                                                                                cTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAgt                                                                                                                        <  7:292233/83‑1 (MQ=255)

                                                                                cTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAgt                                                                                                                        >  8:292233/1‑83 (MQ=255)

                                                                                cTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTc                                                                <  8:37631/139‑1 (MQ=255)

                                                                                cTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTc                                                                >  7:37631/1‑139 (MQ=255)

                                                                                      acaGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGAt                                                            <  8:35270/137‑1 (MQ=255)

                                                                                      acaGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGAt                                                            >  7:35270/1‑137 (MQ=255)

                                                                                           tCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATccc                                           <  5:17683/149‑1 (MQ=255)

                                                                                                      cTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGa                                <  4:272634/149‑1 (MQ=255)

                                                                                                         tgCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTcc                                                                   >  8:268922/1‑111 (MQ=255)

                                                                                                         tgCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTcc                                                                   <  7:268922/111‑1 (MQ=255)

                                                                                                             aGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTgg                         >  1:105332/1‑149 (MQ=255)

                                                                                                                      tCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGttt                      >  7:319341/1‑143 (MQ=255)

                                                                                                                      tCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGttt                      <  8:319341/143‑1 (MQ=255)

                                                                                                                                  gggCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGt    <  2:76247/149‑1 (MQ=255)

                                                                                                                                    gCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGc  <  7:75258/149‑1 (MQ=255)

CCTGCCACCGATAGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTACTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGC  >  NC_002947_CJ‑RC/5395922‑5396202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTACTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGCATGTCTGCACG  >  NC_002947_CJ‑RC/5395934‑5396213

AGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAG                                                                                                                                    <  NB501016:282:H2V7GAFX2:2:21209:17309:2081/150‑1 (MQ=60)

 GATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTG                                                                                                                                  <  NB501016:282:H2V7GAFX2:3:21409:17833:15376/151‑1 (MQ=60)

   TAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCACGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTG                                                                                                                                >  NB501016:282:H2V7GAFX2:2:11211:1479:16719/1‑151 (MQ=60)

    AGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTC                                                                                                                                <  NB501016:282:H2V7GAFX2:1:11308:2510:7663/150‑1 (MQ=60)

              CTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGG                                                                                                                     >  NB501016:282:H2V7GAFX2:3:21504:22340:5844/1‑151 (MQ=60)

                         GCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCT                                                                                                          <  NB501016:282:H2V7GAFX2:4:21508:23140:13728/151‑1 (MQ=60)

                          CCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTG                                                                                                         >  NB501016:282:H2V7GAFX2:2:21307:23707:13327/1‑151 (MQ=60)

                            AGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGG                                                                                                       >  NB501016:282:H2V7GAFX2:4:21501:15631:19870/1‑151 (MQ=60)

                            AGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGG                                                                                                       <  NB501016:282:H2V7GAFX2:2:21209:20556:5814/151‑1 (MQ=60)

                                                GCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAA                                                                                    >  NB501016:282:H2V7GAFX2:1:11206:9478:5902/1‑150 (MQ=60)

                                                          TGCAGCATGGCTACATACAGGTCGTGCTTGAGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTATCTCGACAGCGG                                                                                                                      >  NB501016:282:H2V7GAFX2:4:21404:3253:9671/1‑106 (MQ=60)

                                                                   GCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTG                                                                                                                                  <  NB501016:282:H2V7GAFX2:4:21606:13249:3731/85‑1 (MQ=60)

                                                                   GCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGACGCGCTTGCGCAGTGTCTAGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCCAACAACTGTCCTTCA                                                                          >  NB501016:282:H2V7GAFX2:4:11409:25846:5099/1‑141 (MQ=60)

                                                                         TACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATG                                                                      >  NB501016:282:H2V7GAFX2:4:11408:16331:14256/1‑139 (MQ=60)

                                                                              GTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCG                                                     <  NB501016:282:H2V7GAFX2:3:11405:23444:1986/151‑1 (MQ=60)

                                                                                            GTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCT                                                                             <  NB501016:282:H2V7GAFX2:4:21512:25762:14856/113‑1 (MQ=60)

                                                                                                CAGAACCATGTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGT                                   >  NB501016:282:H2V7GAFX2:1:11301:3159:1159/1‑151 (MQ=60)

                                                                                                         GTCAGCTCCCATGGGGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTA                                >  NB501016:282:H2V7GAFX2:4:21612:11063:8015/1‑145 (MQ=60)

                                                                                                                       GGCCGTCTACTGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGCA            <  NB501016:282:H2V7GAFX2:4:11505:25810:14862/151‑1 (MQ=60)

                                                                                                                                 TGCTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGCATGTCTGCACG  >  NB501016:282:H2V7GAFX2:2:11201:19943:11037/1‑151 (MQ=60)

AGATAGTGCGCTGGCTCCGTTAGGTGCCAGCAGCTTGATGGGGATATGGCCCTGATCGTGCAGCATGGCTACATACAGGTCGTGCTTGCGCTGTGCCAGAACCATGTCAGCTCCCATGGGGCCGTCTACTACTAGGCCGCGCTTGCGCAGTGTCTCGACAGCGGGAACGGTGATCTGGGAGTCCTCCAGGCCGAACAACTGTCCTTCAGATGCATGAGGGTTGATCCCGAATACAGCGACTTTTGGTTTAGGCACTCCGAGTAAGGTGCATGTCTGCACG  >  NC_002947_CJ‑RC/5395934‑5396213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`