Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F18 I1 R1
|
54 |
68.8 |
3145800 |
98.6% |
3101758 |
145.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,070,248 |
+GA |
intergenic (+48/+94) |
aroP‑I → / ← PP_0928 |
aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,070,246 | 1 | . | G | 81.8%
| 88.0
/ 16.0
| 34 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base G (8/19); total (9/25) |
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
| * | NC_002947_CJ‑RC | 1,070,246 | 2 | . | A | 82.3%
| 79.6
/ 16.5
| 34 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base A (9/19); total (9/25) |
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 |
CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGT > NC_002947_CJ‑RC/1070098‑1070374
||
ccTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt < 5:318844/149‑1 (MQ=255)
ccTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt < 8:270248/149‑1 (MQ=255)
cTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt > 8:186365/1‑148 (MQ=255)
aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑a < 6:140162/147‑1 (MQ=255)
gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt > 6:276997/1‑143 (MQ=255)
gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt > 6:115896/1‑143 (MQ=255)
gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑agatc < 2:133985/149‑5 (MQ=255)
gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑agatcgag < 5:312055/149‑8 (MQ=255)
cGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGcgccgc < 5:265454/148‑1 (MQ=255)
gCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCcgcgc > 7:342342/1‑149 (MQ=255)
aTCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCGGTTAG‑‑A‑T‑‑AGAGCGCcccgccg > 5:191666/1‑147 (MQ=255)
tcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCgcgggcg > 7:407199/1‑149 (MQ=255)
ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGggcggc < 2:364997/149‑1 (MQ=255)
ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGggcggc < 7:186365/149‑1 (MQ=255)
ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCATCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCCCGcgcggc < 7:279238/149‑1 (MQ=38)
tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGGGCGCCGCGCGGGCGGCGCTCg > 8:34514/1‑149 (MQ=255)
cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGa < 3:304892/148‑1 (MQ=255)
aTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGAtc < 2:354476/147‑1 (MQ=255)
tCGCGGGGCTGGGGGGGGATTACAAGCCGAAGACGGCCCGCCCGCCCACGGCCTGATCGGGTTTGTTGGTACGGGGTATCCCTTTAGGTTTCCGGCGCCTGT‑‑G‑‑A‑TACCCCCCGCCGCGCGGGCGGCGCCCGATCTCACAGGCGATGaaa < 2:187708/149‑1 (MQ=255)
tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGc < 4:163254/148‑1 (MQ=255)
ggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTTTTCGGGTTTGTTCGTAAGGGGTATGCCGTTTGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCTATCTCAAAGGCGATGAAAGCCTAAAGGCATg < 8:360701/149‑1 (MQ=38)
gtggCGATCAAGCTGCAGATCGCTCGCCAGGCGAAGGGCTGATCGCGTTTCTCCGTCAGGGGTATGCCGTTCGGTTTTCGGGGCCTGT‑‑G‑‑AGTA‑CGAGCGCCGCGCGGGCGGCGCTGGATCTCACAGGCGATGAAAGCCTAAAGGCATgg < 2:232384/149‑2 (MQ=25)
tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTg > 2:253104/1‑149 (MQ=255)
tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGAtctc > 8:96005/1‑115 (MQ=255)
tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGAtctc < 7:96005/115‑1 (MQ=255)
aatgCTGATGATGGCTGGCTTGGCATAGGGCTGATTGGGTTTGTTCGTATGGGTTATGCGGTTTGGTTTTCGGGGCCTGTGAG‑‑A‑T‑‑GGAGGGCCGGGCGGGGGGCGCTCGATCTCAAAGGGGATGAAAGCCTAAAGGCATGCGTCTggc < 6:207196/145‑1 (MQ=255)
gaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGggcggc < 2:84367/95‑1 (MQ=37)
gaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGggcggc > 1:84367/1‑95 (MQ=37)
cAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCtt < 8:342342/149‑1 (MQ=255)
gTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc < 2:328194/148‑1 (MQ=255)
ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTcc < 7:34514/148‑1 (MQ=255)
cGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAATGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGc < 5:115896/148‑1 (MQ=255)
gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑AGAGCGCCCCGCGGGCGGCGCGCGGTCTCACAGGCGATGAAAGCCAAAAGGCATGCGTCTGGCGGCCCGGACGGGGTCCCCTTGCATGCCCCGCAAAGCTGCCTCcccc > 2:281708/1‑148 (MQ=255)
tAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa < 6:191666/149‑1 (MQ=255)
tAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa < 1:253104/149‑1 (MQ=255)
tATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑AGAGCGCCGCGCGGGCGGCGCGCGGTCGCACAGGCGATGAAAGCCAAAAGGCATGCGTATGGCGGCCCGGGCGCGTTCCCCGTGCATGCCCCGCAAATCAGCCTACGCCCAgcacgca > 8:337244/1‑149 (MQ=19)
cGTTAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 8:407199/149‑1 (MQ=255)
tatggTTTTCGCCGCCTGT‑‑GATA‑T‑‑CCTCCGCCGCGGGGGCGGCGCCCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt < 3:254540/146‑1 (MQ=255)
tAGGTTTTCGGCGCCTGTGAG‑‑A‑T‑‑CGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt < 3:196964/148‑1 (MQ=255)
||
CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑A‑T‑‑CGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGT > NC_002947_CJ‑RC/1070098‑1070374
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAC > NC_002947_CJ‑RC/1070106‑1070398
|
CGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGC < NB501016:282:H2V7GAFX2:3:21510:12389:4200/151‑1 (MQ=60)
TCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCG < NB501016:282:H2V7GAFX2:3:21501:25320:7993/150‑1 (MQ=60)
CGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCG > NB501016:282:H2V7GAFX2:4:21512:13841:18277/1‑151 (MQ=60)
CTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGG > NB501016:282:H2V7GAFX2:4:21612:5244:17743/1‑151 (MQ=60)
CGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCG < NB501016:282:H2V7GAFX2:4:11609:20951:16981/151‑1 (MQ=60)
CGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCATCGCCTGTGAG‑‑ATCGAGCGCCGCCCGCGCGGCG < NB501016:282:H2V7GAFX2:4:21501:22578:16325/151‑1 (MQ=60)
GCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGAT < NB501016:282:H2V7GAFX2:2:21210:17349:13201/150‑1 (MQ=60)
GCTGTGGGTGGCTTTCACGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTCCGTAAGGGGTATGCCGTAAGGTTTCCGGCGCCTGTGAG‑‑ATCGCGCCCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGG < NB501016:282:H2V7GAFX2:4:21506:4310:4223/151‑1 (MQ=60)
GGTGGCTTCCACGCTGAAGACGGCCCCCCAGGCCAAGGGCTTATCGGGTTGGTGCGTACGGGGTAGGCCGTTCGGTTTTCGGCGCCGGG‑‑GCCATCGCGCGCCGCGCGGGCGGCGCCCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCG < NB501016:282:H2V7GAFX2:4:11409:12721:8790/151‑1 (MQ=52)
CTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATAGAGCGCCGCGCGGGGGGTGGTCGATCTAAAAGGCGATGAAAtcagatgggaagagcacacc > NB501016:282:H2V7GAFX2:3:11609:1713:4773/1‑131 (MQ=60)
TTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCA < NB501016:282:H2V7GAFX2:4:11505:22458:10010/117‑1 (MQ=60)
AGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCG > NB501016:282:H2V7GAFX2:1:11203:11060:12794/1‑97 (MQ=60)
GTTCGTACGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCG < NB501016:282:H2V7GAFX2:4:11406:23014:18317/150‑1 (MQ=60)
TCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAATGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCC < NB501016:282:H2V7GAFX2:3:11510:8443:4247/150‑1 (MQ=60)
GTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAG < NB501016:282:H2V7GAFX2:1:21110:21814:12108/151‑1 (MQ=60)
TTAGGTTTTCGGCGCCTGTGAG‑‑ATCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTG < NB501016:282:H2V7GAFX2:2:11312:25121:8142/150‑1 (MQ=60)
ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAC > NB501016:282:H2V7GAFX2:1:11304:26387:10942/1‑151 (MQ=60)
ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGGTCCCCTTCCATGCCCCTCAATGCTGCCGGCGCCAAG > NB501016:282:H2V7GAFX2:1:21305:5701:10270/1‑114 (MQ=60)
|
CGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑G‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAC > NC_002947_CJ‑RC/1070106‑1070398
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
