Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F35 I1 R1 74 857.2 37938082 99.4% 37710453 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
MC JC AE015451_phaM1 3,556,355 Δ14,665 bp [PP_3140 CDS (Glycosyl transferase_ group 2 family protein)][yneJ CDS (putative DNA‑binding transcriptional regulator_ LysR‑type)] [PP_3140 CDS (Glycosyl transferase_ group 2 family protein)], PP_3141 CDS (Glycosyl transferase_ WecB_TagA_CpsF family), PP_3142 CDS (putative Sugar transferase), PP_3143 CDS (Transcriptional regulator_ LysR family), tdcG‑III CDS (L‑serine dehydratase), PP_3145 CDS (conserved protein of unknown function), PP_3146 CDS (putative Oxidoreductase), potF‑II CDS (putrescine‑binding periplasmic protein), PP_3148 CDS (putative Glutamine synthetase), PP_3149 CDS (Transcriptional regulator_ AraC family), PP_3150 CDS (conserved protein of unknown function), sad‑II CDS (NAD+‑dependent succinate semialdehyde dehydrogenase), [yneJ CDS (putative DNA‑binding transcriptional regulator_ LysR‑type)]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AE015451_phaM1 3556355 3571019 14665 683 [0] [0] 692 [PP_3140 CDS (Glycosyl transferase_ group 2 family protein)]–[yneJ CDS (putative DNA‑binding transcriptional regulator_ LysR‑type)] [PP_3140 CDS (Glycosyl transferase_ group 2 family protein)],PP_3141 CDS (Glycosyl transferase_ WecB_TagA_CpsF family),PP_3142 CDS (putative Sugar transferase),PP_3143 CDS (Transcriptional regulator_ LysR family),tdcG‑III CDS (L‑serine dehydratase),PP_3145 CDS (conserved protein of unknown function),PP_3146 CDS (putative Oxidoreductase),potF‑II CDS (putrescine‑binding periplasmic protein),PP_3148 CDS (putative Glutamine synthetase),PP_3149 CDS (Transcriptional regulator_ AraC family),PP_3150 CDS (conserved protein of unknown function),sad‑II CDS (NAD+‑dependent succinate semialdehyde dehydrogenase),[yneJ CDS (putative DNA‑binding transcriptional regulator_ LysR‑type)]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 35563540 (0.000)646 (0.800) 121/254 0.2 100% coding (771/1188 nt) PP_3140 CDS (Glycosyl transferase_ group 2 family protein)
?AE015451_phaM1 3571020 = 0 (0.000)coding (49/867 nt) yneJ CDS (putative DNA‑binding transcriptional regulator_ LysR‑type)

GATK/CNVnator alignment

N/A