Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,132,077 G→A A111V (GCG→GTG)  yghQ ← putative inner membrane polysaccharide flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,132,0770GA97.2% 1443.0 / 18.3 434A111V (GCG→GTG) yghQputative inner membrane polysaccharide flippase
Reads supporting (aligned to +/- strand):  ref base G (3/9);  new base A (187/230);  total (192/242)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 441 total aligned reads displayed.

GCGATCTACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCGCCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTGATCGACTTG  >  NC_000913/3131929‑3132223
                                                                                                                                                    |                                                                                                                                                  
  gATCTACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATcaccac                                                                                                                                                >  1:778321/1‑151 (MQ=255)
   aTCTACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCaccacc                                                                                                                                               >  1:1186581/1‑151 (MQ=255)
     cTACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACcgc                                                                                                                                             >  7:1095463/1‑151 (MQ=255)
      tACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCCCCGACAATCACCACcgcg                                                                                                                                            >  2:1027258/1‑151 (MQ=255)
       aCCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACcg                                                                                                                                              <  4:963924/148‑1 (MQ=255)
       aCCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACcg                                                                                                                                              <  7:842655/148‑1 (MQ=255)
        ccGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGcgcc                                                                                                                                          >  1:1574147/1‑151 (MQ=255)
         cgcacgcaGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGcgccg                                                                                                                                         >  8:441311/1‑151 (MQ=255)
           cacgcaGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCt                                                                                                                                       >  2:274749/1‑151 (MQ=255)
                aGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACg                                                                                                                                   >  4:1656888/1‑150 (MQ=255)
                  aaTGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGat                                                                                                                                 >  4:812189/1‑150 (MQ=255)
                     gCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATc                                                                                                                              <  3:1424110/150‑1 (MQ=255)
                      gcGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGTAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCa                                                                                                                            <  2:1214875/150‑1 (MQ=255)
                          tCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCg                                                                                                                         >  2:1519509/1‑150 (MQ=255)
                            ggCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCgaga                                                                                                                      <  8:1257242/151‑1 (MQ=255)
                            ggCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCg                                                                                                                         >  1:694820/1‑148 (MQ=255)
                             gCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGaa                                                                                                                     <  7:1185777/151‑1 (MQ=255)
                              cGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCgaga                                                                                                                      >  5:1512621/1‑149 (MQ=255)
                              cGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAg                                                                                                                    <  6:539469/151‑1 (MQ=255)
                                 ggaggaAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGa                                                                                                                  >  4:891256/1‑150 (MQ=255)
                                  gatgaAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGaa                                                                                                                 <  8:816040/147‑1 (MQ=255)
                                  gaggaAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGaa                                                                                                                 >  8:234053/1‑150 (MQ=255)
                                      aaGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATg                                                                                                             >  8:1491028/1‑150 (MQ=255)
                                      aaGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCCACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATg                                                                                                             >  8:362804/1‑150 (MQ=255)
                                      aaGCCATTGAAGGAATGACCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATCGTAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATga                                                                                                            <  5:3711/151‑1 (MQ=255)
                                       aGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATgag                                                                                                           <  6:1577635/151‑1 (MQ=255)
                                           aTTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAgacga                                                                                                       <  6:1354102/151‑1 (MQ=255)
                                           aTTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAgacga                                                                                                       <  8:29741/151‑1 (MQ=255)
                                               atgtaaTAAGCGTTCAATAGACCGCGGCCAGCCAAAAACTTTGTTCTTCTAGACCTAATGAATGGGAACGGATTGGCAGAAAGGCAAGGCCACCGACATTCAGCACCGCGCCGCTGACGATATCCAGCGATATGGAAAATGAGACGACAtt                                                                                                   <  1:456370/147‑1 (MQ=255)
                                               aaGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACAtt                                                                                                   >  5:781333/1‑151 (MQ=255)
                                                   aaTGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCgg                                                                                               <  3:305325/151‑1 (MQ=255)
                                                     tGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCCCTGACGATATCCAGCGAGAAGGACAATGAGACGACATTGCGGa                                                                                              >  3:996583/1‑150 (MQ=255)
                                                      gAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGaa                                                                                             <  4:1554706/150‑1 (MQ=255)
                                                      gAGCGTGCAATACTGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGaa                                                                                             <  3:778386/150‑1 (MQ=255)
                                                       aGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAAtt                                                                                           <  7:97057/151‑1 (MQ=255)
                                                       aGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAAtt                                                                                           >  6:623348/1‑151 (MQ=255)
                                                         cGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATtgc                                                                                         <  4:48238/151‑1 (MQ=255)
                                                             cAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCg                                                                                     <  4:1448025/151‑1 (MQ=255)
                                                             cAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCg                                                                                     <  3:1095590/151‑1 (MQ=255)
                                                              aaTAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCgg                                                                                    >  3:460936/1‑151 (MQ=255)
                                                                 agagCGCTGCCAGCCAAAAAGTTTGGTCATCCAGAACTAATGAATGGGAAAGGAATGGGAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGatt                                                                                 <  6:233712/151‑1 (MQ=255)
                                                                 agagCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGatt                                                                                 >  1:568243/1‑151 (MQ=255)
                                                                   agCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGatta                                                                                <  6:379921/150‑1 (MQ=255)
                                                                        tGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTAttgttg                                                                          >  7:381486/1‑151 (MQ=255)
                                                                         gccagccaAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTgg                                                                         <  2:750539/151‑1 (MQ=255)
                                                                          ccagccaAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGt                                                                        <  8:1662787/151‑1 (MQ=255)
                                                                              ccaAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAAt                                                                    <  3:883477/151‑1 (MQ=255)
                                                                               caAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATg                                                                   >  2:820557/1‑151 (MQ=255)
                                                                                aaaaaCTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGc                                                                  >  7:1489817/1‑151 (MQ=255)
                                                                                   aaCTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACTGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTgg                                                               <  1:142426/151‑1 (MQ=255)
                                                                                   aaCTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTgg                                                               <  6:1682259/151‑1 (MQ=255)
                                                                                        tGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGtt                                                           <  1:1020725/150‑1 (MQ=255)
                                                                                         ggTCATCCAGACATAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGACACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTcc                                                         <  5:894284/151‑1 (MQ=255)
                                                                                            cATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGt                                                       >  4:223143/1‑150 (MQ=255)
                                                                                               ccAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCGCCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACt                                                    <  6:324850/150‑1 (MQ=255)
                                                                                               ccAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACt                                                    <  3:1213875/150‑1 (MQ=255)
                                                                                               ccAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTg                                                   >  1:227656/1‑151 (MQ=255)
                                                                                               ccAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTg                                                   >  5:1267040/1‑151 (MQ=255)
                                                                                                cAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTg                                                   >  4:1141024/1‑150 (MQ=255)
                                                                                                 aGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGCCAATCACCACCGCGCCGCTGCCGATATCCAGCGAGAAGGAAAATGAGACGACATCGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGa                                                  >  4:1325182/1‑150 (MQ=255)
                                                                                                  gACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAac                                                >  1:939651/1‑151 (MQ=255)
                                                                                                  gACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAac                                                >  8:139980/1‑151 (MQ=255)
                                                                                                    ccTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAacc                                               >  8:526136/1‑150 (MQ=255)
                                                                                                       aatgactgGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAaccacc                                            <  6:1445639/150‑1 (MQ=255)
                                                                                                       aatgaatgGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAaccacc                                            >  8:913349/1‑150 (MQ=255)
                                                                                                       aatgaatgGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAaccacc                                            <  1:1141620/150‑1 (MQ=255)
                                                                                                         tgaatgGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCTCCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGt                                         >  4:717989/1‑151 (MQ=255)
                                                                                                            atgGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGc                                      <  5:1664768/151‑1 (MQ=255)
                                                                                                              gggAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGcc                                     <  5:808333/150‑1 (MQ=255)
                                                                                                              gggAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCa                                    <  3:1662361/151‑1 (MQ=255)
                                                                                                               ggAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGCCCCACCAGTTGCCAt                                   >  2:470314/1‑151 (MQ=255)
                                                                                                                gAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATg                                  <  6:969394/151‑1 (MQ=255)
                                                                                                                 aaaGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGt                                 <  8:1300641/151‑1 (MQ=255)
                                                                                                                 aaaGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGt                                 >  2:559726/1‑151 (MQ=255)
                                                                                                                  aaGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTc                                <  3:1261371/151‑1 (MQ=255)
                                                                                                                   aGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCt                               >  3:711809/1‑151 (MQ=255)
                                                                                                                   aGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCt                               >  7:1349813/1‑151 (MQ=255)
                                                                                                                   aGGAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCt                               >  5:589758/1‑151 (MQ=255)
                                                                                                                    ggAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTg                              <  6:1273560/151‑1 (MQ=255)
                                                                                                                     gAATGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACAACCAGTTGCCATGTCTg                              >  5:1387712/1‑150 (MQ=255)
                                                                                                                      aaTGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTGTGa                             >  2:145406/1‑150 (MQ=255)
                                                                                                                      aaTGGCAGTAAGGAAATGCCACCGCCAATCACCACCGGGCCGCTGACGATATCCAGCGAGACGGAAAATTAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGaa                            <  5:68942/151‑1 (MQ=255)
                                                                                                                       aTGGCAGTAAGGCAATGCCACCGACAATCACCCCCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGaaa                           <  1:870860/151‑1 (MQ=255)
                                                                                                                       aTGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGaaa                           <  5:1135300/151‑1 (MQ=255)
                                                                                                                        tGGCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAAc                          >  7:544019/1‑151 (MQ=255)
                                                                                                                         ggCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAAc                          <  4:619724/150‑1 (MQ=255)
                                                                                                                         ggCAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACt                         <  5:298600/151‑1 (MQ=255)
                                                                                                                           cAGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACtt                        >  8:679339/1‑150 (MQ=255)
                                                                                                                            attaattctttGCCACCTTCAATCAACACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTta                       <  6:763594/140‑1 (MQ=255)
                                                                                                                            aGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTta                       >  7:681328/1‑150 (MQ=255)
                                                                                                                            aGTAAGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTta                       >  7:1621945/1‑150 (MQ=255)
                                                                                                                                aGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTtaataa                   >  2:196757/1‑150 (MQ=255)
                                                                                                                                aGGCAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATaaa                  <  4:401802/151‑1 (MQ=255)
                                                                                                                                   cAATGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATc               >  3:1553582/1‑151 (MQ=255)
                                                                                                                                      tGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCt            >  8:1050729/1‑151 (MQ=255)
                                                                                                                                      tGCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATAGCTGCGGATTATTCTTGCTTAATGCTGGTGTTCCGTACTGAACCACCAGTAGCCATGTCTGAAACTTAATAAAATCGCt            >  6:845554/1‑151 (MQ=255)
                                                                                                                                       gCCACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTg           >  7:29895/1‑151 (MQ=255)
                                                                                                                                        ccACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTg           >  6:1118901/1‑150 (MQ=255)
                                                                                                                                         cACCGACAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTGAt         >  6:139755/1‑151 (MQ=255)
                                                                                                                                              aCAATCACCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTGATCGACt    <  7:1667390/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGATCTACCGCACGCAGAATGCCGGTCGGCGTGGAGGAAGCCATTGAAGGAATGAGCGTGCAATAGAGCGCTGCCAGCCAAAAACTTTGGTCATCCAGACCTAATGAATGGGAAAGGAATGGCAGTAAGGCAATGCCACCGACAATCGCCACCGCGCCGCTGACGATATCCAGCGAGAAGGAAAATGAGACGACATTGCGGAATTGCTGCGGATTATTGTTGGTTAATGCTGGTGTTCCGTACTGAACCACCAGTTGCCATGTCTGAAACTTAATAAAATCGCTGATCGACTTG  >  NC_000913/3131929‑3132223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A