Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,133,175 G→A T13I (ACC→ATC)  yghR ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,133,1750GA95.5% 1385.0 / 34.8 422T13I (ACC→ATC) yghRputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (7/12);  new base A (190/212);  total (197/225)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 423 total aligned reads displayed.

TCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGG  >  NC_000913/3133025‑3133322
                                                                                                                                                      |                                                                                                                                                   
   tCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGAtt                                                                                                                                                   <  2:514111/150‑1 (MQ=255)
   tCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGAtt                                                                                                                                                   <  8:186609/150‑1 (MQ=255)
      ccGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTAc                                                                                                                                               <  5:1179622/151‑1 (MQ=255)
          agagCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTAtt                                                                                                                                           <  3:693693/151‑1 (MQ=255)
          agagCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTAtt                                                                                                                                           >  1:166808/1‑151 (MQ=255)
          agagCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTAtt                                                                                                                                           <  1:212964/151‑1 (MQ=255)
          agagCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGNGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTAtt                                                                                                                                           >  4:532071/1‑151 (MQ=255)
           gagCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTa                                                                                                                                          <  1:501457/151‑1 (MQ=255)
             gcagcCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAAc                                                                                                                                        >  6:83329/1‑151 (MQ=255)
              cagcggctgATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGAGCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACa                                                                                                                                       <  6:1062693/143‑1 (MQ=255)
                   ccAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACagtttg                                                                                                                                  >  1:363264/1‑151 (MQ=255)
                   ccAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACagtttg                                                                                                                                  >  8:161379/1‑151 (MQ=255)
                         aGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACagtttgagtttg                                                                                                                            >  6:587644/1‑151 (MQ=255)
                          gcgcCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGt                                                                                                                           <  4:251217/151‑1 (MQ=255)
                          gcgcCGCTCGGGTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGt                                                                                                                           <  5:569512/151‑1 (MQ=255)
                           cgccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTa                                                                                                                          <  1:1340932/151‑1 (MQ=255)
                           cgccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGAGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTa                                                                                                                          <  2:324917/151‑1 (MQ=255)
                            gccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGt                                                                                                                           >  3:1355286/1‑149 (MQ=255)
                            gccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGt                                                                                                                           >  3:1168396/1‑149 (MQ=255)
                            gccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAg                                                                                                                         <  8:1471336/151‑1 (MQ=255)
                             ccgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGt                                                                                                                        >  5:1260859/1‑151 (MQ=255)
                              cgcTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTg                                                                                                                       >  8:1048768/1‑151 (MQ=255)
                                   ggTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATcc                                                                                                                  >  6:99861/1‑151 (MQ=255)
                                   ggTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATcc                                                                                                                  <  1:1459785/151‑1 (MQ=255)
                                    gTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCa                                                                                                                 <  4:872372/151‑1 (MQ=255)
                                      ttGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCAt                                                                                                                <  3:271941/150‑1 (MQ=255)
                                         ccAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGt                                                                                                            <  5:244030/151‑1 (MQ=255)
                                           aGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGGTTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTa                                                                                                          <  2:1104939/151‑1 (MQ=255)
                                           aGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTa                                                                                                          <  4:1480569/151‑1 (MQ=255)
                                             tgttgtTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTa                                                                                                          >  3:1653479/1‑149 (MQ=255)
                                                 gttgCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAatca                                                                                                     <  1:851007/150‑1 (MQ=255)
                                                 gttgCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAatcat                                                                                                    <  8:136777/151‑1 (MQ=255)
                                                    gCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATcc                                                                                                  <  2:721341/150‑1 (MQ=255)
                                                    gCAGCGATTTCACCAGGTCGGTGGTCAGTCTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATcc                                                                                                  <  2:862441/150‑1 (MQ=255)
                                                         gATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATc                                                                                            >  5:323205/1‑151 (MQ=255)
                                                         gATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATc                                                                                            >  1:63280/1‑151 (MQ=255)
                                                            ttCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAt                                                                                         >  4:1308489/1‑151 (MQ=255)
                                                              cACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAttt                                                                                       <  1:1604125/151‑1 (MQ=255)
                                                              cACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAttt                                                                                       >  3:82067/1‑151 (MQ=255)
                                                              cACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAttt                                                                                       >  3:969865/1‑151 (MQ=255)
                                                               aCCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAtttt                                                                                      >  6:1180901/1‑151 (MQ=255)
                                                               aCCAGGGCGGTGGTCAGTGGGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCAtttg                                                                                      <  1:1240816/151‑2 (MQ=255)
                                                                  aGGTCGGTGGTCAGTGTGGATTTAGCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGt                                                                                   <  3:1497903/151‑1 (MQ=255)
                                                                        gtggtCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAg                                                                             <  8:518508/151‑1 (MQ=255)
                                                                         aggtCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAgg                                                                            >  6:163290/2‑151 (MQ=255)
                                                                              aGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAgc                                                                       <  6:926404/151‑1 (MQ=255)
                                                                              aGTGTGGATTTACCGGGGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAgc                                                                       <  8:858648/151‑1 (MQ=255)
                                                                               gtgtGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAgcg                                                                      <  2:1650318/151‑1 (MQ=255)
                                                                                tgtgGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAgcgc                                                                     >  1:720781/1‑151 (MQ=255)
                                                                                 gtgGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGcgcg                                                                    >  5:472698/1‑151 (MQ=255)
                                                                                   ggATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCgcgg                                                                   <  8:1250347/150‑1 (MQ=255)
                                                                                   ggATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCgcgg                                                                   <  5:690475/150‑1 (MQ=255)
                                                                                     aTTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGt                                                                <  5:112052/151‑1 (MQ=255)
                                                                                     aTTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGt                                                                <  7:1636303/151‑1 (MQ=255)
                                                                                          ccGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAAtt                                                           >  5:937408/1‑151 (MQ=255)
                                                                                            ggTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTgc                                                         <  8:1100184/151‑1 (MQ=255)
                                                                                               gCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGcgcg                                                      <  1:1109304/151‑1 (MQ=255)
                                                                                                 cATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTa                                                    <  7:1096180/151‑1 (MQ=255)
                                                                                                 cATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTa                                                    <  6:1275073/151‑1 (MQ=255)
                                                                                                 cATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTa                                                    <  1:923216/151‑1 (MQ=255)
                                                                                                    cacaCCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTTCGGCGCGATTGTACTATTAACAGTTTGATTGTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGt                                                  <  2:1264114/150‑1 (MQ=255)
                                                                                                     acacCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTg                                                 >  4:192208/1‑150 (MQ=255)
                                                                                                     acacCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGa                                                <  4:1240780/151‑1 (MQ=255)
                                                                                                     aaacCCGACCACCGCAATCAGACCCCGAATGTAGTTGGGCTGCACCGCGATTGTACTATTAACAGTTTGAGTATGTAGTGCATCCATTCGTTAAATCATCCACTATCCAATTTGCTTATCACCAAAGCGCGGCCAGAATTGCGCGTAGTg                                                 >  6:747260/3‑150 (MQ=255)
                                                                                                        cccGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGc                                             <  5:1526817/151‑1 (MQ=255)
                                                                                                         ccGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCt                                            >  7:1393514/1‑151 (MQ=255)
                                                                                                          cGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCtt                                           >  6:297797/1‑151 (MQ=255)
                                                                                                           gACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTg                                          >  5:815466/1‑151 (MQ=255)
                                                                                                           gACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTg                                          <  6:503973/151‑1 (MQ=255)
                                                                                                              caccGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGGATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGAGCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGc                                         >  2:1592001/1‑149 (MQ=255)
                                                                                                               accGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGc                                         >  7:513209/1‑148 (MQ=255)
                                                                                                                 cGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAAt                                    <  1:1001740/151‑1 (MQ=255)
                                                                                                                  gCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAAtt                                   >  7:1515962/1‑151 (MQ=255)
                                                                                                                  gCAATCACCCGCGGAATGTAGGTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAATCGCCGCGTGAATTGCGCGTAGTGACGCTTGCAGAAtt                                   <  3:773186/151‑1 (MQ=255)
                                                                                                                    aaTCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAAttt                                  >  4:670839/1‑150 (MQ=255)
                                                                                                                    aaTCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTc                                 <  5:159045/151‑1 (MQ=255)
                                                                                                                    aaTCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTc                                 >  1:783818/1‑151 (MQ=255)
                                                                                                                    aaTCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTc                                 >  7:72396/1‑151 (MQ=255)
                                                                                                                        aGCCCCGGAATTTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcg                             >  5:1386924/1‑151 (MQ=255)
                                                                                                                        aGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcg                             <  8:91600/151‑1 (MQ=255)
                                                                                                                        aGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcg                             >  8:629591/1‑151 (MQ=255)
                                                                                                                        aGCCCCGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcg                             <  5:1498424/151‑1 (MQ=255)
                                                                                                                          ccccGGAAGGTAGTTGGGCTGAGGCGCGATTGTACTATTAACAGTTTGAGTGTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGCTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcggc                           <  2:658520/151‑1 (MQ=255)
                                                                                                                            ccGGAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATcggcgg                         <  5:1047066/151‑1 (MQ=255)
                                                                                                                              ggAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCgggg                       >  4:1074698/1‑151 (MQ=255)
                                                                                                                               gAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgt                      <  7:302144/151‑1 (MQ=255)
                                                                                                                               gAATGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgt                      >  5:27487/1‑151 (MQ=255)
                                                                                                                                aaTGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg                     <  3:356353/151‑1 (MQ=255)
                                                                                                                                  tGTAGTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTc                   >  7:1599401/1‑151 (MQ=255)
                                                                                                                                      gTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGccc               <  3:1278873/151‑1 (MQ=255)
                                                                                                                                      gTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGccc               <  6:631373/151‑1 (MQ=255)
                                                                                                                                      gTTGGGCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGccc               <  2:1007715/151‑1 (MQ=255)
                                                                                                                                         gggCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCAt             <  7:1246723/150‑1 (MQ=255)
                                                                                                                                         gcggtGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCGATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATc            <  6:1643879/147‑1 (MQ=255)
                                                                                                                                          ggCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc           >  7:909136/1‑151 (MQ=255)
                                                                                                                                           gCTGCGGCGCGTTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAACCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCa          <  3:891587/151‑1 (MQ=255)
                                                                                                                                           gCTGCGGCGCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCa          <  8:1219055/151‑1 (MQ=255)
                                                                                                                                                ggcgCGATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCa     <  3:453517/151‑1 (MQ=255)
                                                                                                                                                   gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg  <  2:1492860/151‑1 (MQ=255)
                                                                                                                                                   gcgATTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg  <  1:1172318/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TCTTCGCCGGAGAGCAGCCCCAGATAGCGCCGCTCGGTTTGCCAGTGTTGTTGCAGCGATTTCACCAGGTCGGTGGTCAGTGTGGATTTACCGGTGCCATCACACCCGACCACCGCAATCAGCCCCGGAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGG  >  NC_000913/3133025‑3133322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A