Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,139,298 G→A A137A (GCC→GCT)  fsaB ← fructose‑6‑phosphate aldolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,139,2980GA97.7% 1468.8 / 11.5 432A137A (GCC→GCT) fsaBfructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base A (202/219);  total (207/225)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 433 total aligned reads displayed.

GCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCAC  >  NC_000913/4139150‑4139447
                                                                                                                                                    |                                                                                                                                                     
  aGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGAGCGTGCATTTCTAACAGCGATTGCAGCTCCTGATCCGTACGAATCCCGTCTCCGCCCTGAGCAt                                                                                                                                                   >  4:239708/1‑151 (MQ=255)
   gggTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATc                                                                                                                                                  >  8:497819/1‑151 (MQ=255)
   gggTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATc                                                                                                                                                  >  6:349308/1‑151 (MQ=255)
     gTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCTGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTTGCNCGTGCNTTTCTAACAGCGTTTGNAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTa                                                                                                                                                <  5:931770/151‑1 (MQ=255)
     gTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTa                                                                                                                                                <  7:41198/151‑1 (MQ=255)
       gATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTAcg                                                                                                                                              <  5:6878/151‑1 (MQ=255)
           gATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGt                                                                                                                                          >  6:1011714/1‑151 (MQ=255)
              tCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTa                                                                                                                                        <  2:533603/150‑1 (MQ=255)
                acaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCAGCATGATTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCACCGCCATGAGCATCTACGAGGTTAAc                                                                                                                                      >  4:1337426/1‑150 (MQ=38)
                acaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACa                                                                                                                                     <  7:272506/151‑1 (MQ=255)
                acaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACa                                                                                                                                     <  1:1353486/151‑1 (MQ=255)
                   tCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGAATCTACGCGGTTAACATAc                                                                                                                                  >  5:1401553/1‑151 (MQ=255)
                    ccTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACg                                                                                                                                 <  4:1131207/151‑1 (MQ=255)
                     cTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACgg                                                                                                                                >  8:1370345/1‑151 (MQ=255)
                       gCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAg                                                                                                                              <  1:1687448/151‑1 (MQ=255)
                        ccAGTAAACAGTCCAGCGCCTGACGGGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACTGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGc                                                                                                                             <  4:1513231/151‑1 (MQ=255)
                        ccAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGc                                                                                                                             >  5:1316074/1‑151 (MQ=255)
                         cAGTAAACAGTCCAGCGCCTGACGCTGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCa                                                                                                                            <  1:974640/151‑1 (MQ=255)
                         cAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGACTGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGc                                                                                                                             >  5:824316/1‑150 (MQ=255)
                         cAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGc                                                                                                                             >  7:1018412/1‑150 (MQ=255)
                         cAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCa                                                                                                                            <  6:76337/151‑1 (MQ=255)
                          aGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGGACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCa                                                                                                                            >  1:355233/1‑150 (MQ=255)
                          aGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCaa                                                                                                                           <  3:1350802/151‑1 (MQ=255)
                          aGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCa                                                                                                                            >  6:665975/1‑150 (MQ=255)
                            tAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACg                                                                                                                         >  4:828517/1‑151 (MQ=255)
                             aaaCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGt                                                                                                                        >  2:303530/1‑151 (MQ=255)
                              aaCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTa                                                                                                                       <  7:781642/151‑1 (MQ=255)
                                    ccAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGc                                                                                                                 >  7:659495/1‑151 (MQ=255)
                                         gcCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCACTg                                                                                                            >  8:1054868/1‑151 (MQ=255)
                                            tGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGcc                                                                                                          >  3:289236/1‑150 (MQ=255)
                                             gACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCa                                                                                                         <  1:1028901/150‑1 (MQ=255)
                                               cgcgGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTg                                                                                                      >  5:553167/1‑151 (MQ=255)
                                                 gggcgTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCg                                                                                                    <  4:585256/150‑1 (MQ=255)
                                                     gTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCt                                                                                                 >  7:866440/1‑150 (MQ=255)
                                                     gTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCt                                                                                                 >  4:901725/1‑150 (MQ=255)
                                                      ttttAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCta                                                                                                >  5:503464/1‑150 (MQ=255)
                                                         tAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCttata                                                                                             >  6:507879/1‑150 (MQ=255)
                                                         tAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCtaata                                                                                             <  2:829678/150‑1 (MQ=255)
                                                          aaaGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAc                                                                                           >  2:1049027/1‑151 (MQ=255)
                                                              ctggctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCtt                                                                                       >  7:1270790/1‑151 (MQ=255)
                                                                ggctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTtgt                                                                                     <  5:1236772/151‑1 (MQ=255)
                                                                 gctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTtgtg                                                                                    >  5:1110321/1‑151 (MQ=255)
                                                                 gctgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTtgtg                                                                                    <  5:1444799/151‑1 (MQ=255)
                                                                    gCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcg                                                                                  <  7:208031/150‑1 (MQ=255)
                                                                    gCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTAGCCCCTGCCAGTGCGGCTAATAACCCTTGTgc                                                                                   <  2:371031/149‑1 (MQ=255)
                                                                     ccAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAGCCTTGTgcggc                                                                                >  2:747005/1‑151 (MQ=255)
                                                                     ccAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcggc                                                                                <  8:297853/151‑1 (MQ=255)
                                                                       aGCACCATACTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGgcgc                                                                              >  1:1236214/1‑151 (MQ=255)
                                                                           ccATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtata                                                                          <  2:1611780/151‑1 (MQ=255)
                                                                            cATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtatat                                                                         >  1:563184/1‑151 (MQ=255)
                                                                             aTGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtatata                                                                        <  5:40410/151‑1 (MQ=255)
                                                                              tGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAc                                                                       >  2:616161/1‑151 (MQ=255)
                                                                               gCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAc                                                                       >  3:512612/1‑150 (MQ=255)
                                                                                  ttCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAcagc                                                                    <  4:1403244/150‑1 (MQ=255)
                                                                                   tCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAcagca                                                                   <  3:1195503/150‑1 (MQ=255)
                                                                                    cTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGATATATAcagcact                                                                 >  6:231439/1‑149 (MQ=255)
                                                                                      ggCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAcagcag                                                                  <  3:1110990/148‑1 (MQ=255)
                                                                                        cgcgTGCATTTCTAAGAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGcc                                                              >  8:1196860/1‑150 (MQ=255)
                                                                                        cgcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGcc                                                              >  2:1428867/1‑150 (MQ=255)
                                                                                        cgcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGcc                                                              >  4:324221/1‑150 (MQ=255)
                                                                                         gcgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCa                                                             >  6:1200738/1‑150 (MQ=255)
                                                                                          cgTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCa                                                             >  8:86027/1‑149 (MQ=255)
                                                                                           gTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgt                                                          >  8:709554/1‑151 (MQ=255)
                                                                                             gCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtg                                                         <  4:148878/150‑1 (MQ=255)
                                                                                              cATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt                                                        >  4:94105/1‑150 (MQ=255)
                                                                                               aTTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtag                                                      <  3:1589460/151‑1 (MQ=255)
                                                                                                 ttCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagt                                                     <  6:1698423/150‑1 (MQ=255)
                                                                                                 ttCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagt                                                     <  4:984639/150‑1 (MQ=255)
                                                                                                 ttCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagt                                                     >  3:614059/1‑150 (MQ=255)
                                                                                                  tCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTgtagta                                                    <  8:222958/150‑1 (MQ=255)
                                                                                                     aaCAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATa                                                 <  1:727315/150‑1 (MQ=255)
                                                                                                             ttGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCttt                                         <  8:768911/150‑1 (MQ=255)
                                                                                                              tGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCtttt                                        <  6:1431973/150‑1 (MQ=255)
                                                                                                                 aGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTc                                     >  7:1139274/1‑150 (MQ=255)
                                                                                                                     ccTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTAt                                >  8:62217/1‑151 (MQ=255)
                                                                                                                     ccTGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTAt                                >  1:1432155/1‑151 (MQ=255)
                                                                                                                       tGAACCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTAttt                              >  5:1597804/1‑151 (MQ=255)
                                                                                                                         aaCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                            <  4:64074/151‑1 (MQ=255)
                                                                                                                         aaCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                            <  7:139342/151‑1 (MQ=255)
                                                                                                                         aaCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                            <  4:1022104/151‑1 (MQ=255)
                                                                                                                         aaCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                            <  6:1293458/151‑1 (MQ=255)
                                                                                                                          aCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTaa                           <  1:234175/151‑1 (MQ=255)
                                                                                                                          aCCGTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTa                            >  5:300389/1‑150 (MQ=255)
                                                                                                                             gTACGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtg                        <  3:424485/151‑1 (MQ=255)
                                                                                                                                cGAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtgctg                     <  7:955452/151‑1 (MQ=255)
                                                                                                                                 gAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCTCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtgctgc                    <  1:826570/151‑1 (MQ=255)
                                                                                                                                 gAATGCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtgctgc                    >  8:131239/1‑151 (MQ=255)
                                                                                                                                     gCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGcc                   >  8:1641712/1‑148 (MQ=255)
                                                                                                                                     gCCGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAgc                >  1:1107105/1‑150 (MQ=255)
                                                                                                                                      ccGTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGAc               <  1:482495/151‑1 (MQ=255)
                                                                                                                                        gTCTCCGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCt             <  3:1483469/151‑1 (MQ=255)
                                                                                                                                            ccGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTc           <  7:923618/149‑1 (MQ=255)
                                                                                                                                            ccGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCg          <  6:856541/150‑1 (MQ=255)
                                                                                                                                             cGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCgg         >  2:169450/1‑150 (MQ=255)
                                                                                                                                             cGCCCTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCACTGCCAGTGCGGCTAATAACCCTTGTGCTGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTAgg         >  2:1292913/1‑150 (MQ=255)
                                                                                                                                               cccTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAg      <  5:1661756/151‑1 (MQ=255)
                                                                                                                                                ccTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGt     >  3:926689/1‑151 (MQ=255)
                                                                                                                                                ccTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGt     <  8:66565/151‑1 (MQ=255)
                                                                                                                                                 cTGAGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTc    >  2:1573119/1‑151 (MQ=255)
                                                                                                                                                   gggCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCAc  >  4:1652917/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCAC  >  NC_000913/4139150‑4139447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A