Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,530,797 C→T Q285Q (CAG→CAA)  sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,530,7970CT95.7% 1140.8 / 25.2 352Q285Q (CAG→CAA) sgcXputative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base T (190/146);  total (198/154)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 352 total aligned reads displayed.

CAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTG  >  NC_000913/4530649‑4530944
                                                                                                                                                     |                                                                                                                                                   
 aaCCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTGTCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGt                                                                                                                                                  >  2:1588245/1‑151 (MQ=255)
 aaCCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGt                                                                                                                                                  >  7:1090101/1‑151 (MQ=255)
  aCCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGtt                                                                                                                                                 <  5:544230/151‑1 (MQ=255)
    cTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTcc                                                                                                                                               >  3:276048/1‑151 (MQ=255)
            gataaTGGGTATAGCGACAAGGAATAGAGAGACTGGCTCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTGCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCg                                                                                                                                       <  2:1151574/148‑1 (MQ=255)
             agaATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGt                                                                                                                                      >  7:213852/1‑151 (MQ=255)
                  gggTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCa                                                                                                                                  <  5:1602673/150‑1 (MQ=255)
                  gggTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAg                                                                                                                                 <  5:1425900/151‑1 (MQ=255)
                   ggTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAgg                                                                                                                                <  7:543915/151‑1 (MQ=255)
                        aGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCgagg                                                                                                                           >  1:1540296/1‑151 (MQ=255)
                         gctaCAAGGAATAGAGAGACTGGCGCAGTGAATACCATCCTGCTCATCCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCgagg                                                                                                                           <  1:1425968/147‑1 (MQ=255)
                         gCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCgagga                                                                                                                          <  8:341578/151‑1 (MQ=255)
                          cGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCgaggag                                                                                                                         <  8:678431/151‑1 (MQ=255)
                          cGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCgaggag                                                                                                                         <  1:1170256/151‑1 (MQ=255)
                          cGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCgagg                                                                                                                           >  2:54197/1‑149 (MQ=255)
                            aCAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTTAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGc                                                                                                                       <  6:1477039/151‑1 (MQ=255)
                              aaGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGt                                                                                                                     >  2:287233/1‑151 (MQ=255)
                               aGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTg                                                                                                                    <  3:622179/151‑1 (MQ=255)
                               aGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTg                                                                                                                    <  5:462751/151‑1 (MQ=255)
                                 gAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCCGTTTCTGTTATCACGCCGGG‑AGCCACTTCACGCTGCACCGGAATGTTGTGTTCAAGACCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGAt                                                                                                                  >  8:1132240/1‑151 (MQ=255)
                                  aaTAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGAtt                                                                                                                 >  3:870684/1‑151 (MQ=255)
                                  aaTAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGAtt                                                                                                                 <  3:348218/151‑1 (MQ=255)
                                       agagaCTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATcc                                                                                                            >  4:322894/1‑151 (MQ=255)
                                        gagaCTTGCTCAGGGAATACCATCCTGGTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACAGGAATGTTGTGTTCAAGTGCCGTTTGTTCCAAGATCCGTATCAGGCGAGGAGGCGTGATTAATCCg                                                                                                           <  6:554225/151‑1 (MQ=255)
                                            atGGCGCATCTAATACCATCATGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGGGTTCAAGAGCCGTTTGTTCCATCATCCGTATCAGGCGAGGAGGCGTGATTAATccttcca                                                                                                       <  4:1222786/150‑6 (MQ=255)
                                               gcgcAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCg                                                                                                    <  7:777888/151‑1 (MQ=255)
                                                cgcAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGt                                                                                                   >  5:43031/1‑151 (MQ=255)
                                                  cAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTc                                                                                                 <  4:1068696/151‑1 (MQ=255)
                                                            cATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATg                                                                                       <  7:1222361/151‑1 (MQ=255)
                                                               ccTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGt                                                                                     <  4:1014959/150‑1 (MQ=255)
                                                               ccTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGt                                                                                     >  3:1292572/1‑150 (MQ=255)
                                                               ccTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTa                                                                                    <  8:866592/151‑1 (MQ=255)
                                                                cTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTat                                                                                   >  1:359440/1‑150 (MQ=255)
                                                                     caacgtgcttataACCGGTTCCTG‑TAACAAGCCGGGCAGCAACTTCCCGCTGCACGGTAATGTTG‑GGTCCAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcagg                                                                             <  6:271676/142‑1 (MQ=255)
                                                                     cAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcag                                                                              <  5:891030/151‑1 (MQ=255)
                                                                     cAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTc                                                                                <  2:1229201/149‑1 (MQ=255)
                                                                      aaCCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcagg                                                                             >  2:728204/1‑151 (MQ=255)
                                                                       aCCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGTCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggc                                                                            >  5:1670941/1‑151 (MQ=255)
                                                                        ccTGTATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggca                                                                           <  1:294360/151‑1 (MQ=255)
                                                                        ccTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggca                                                                           <  3:558789/151‑1 (MQ=255)
                                                                        ccTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggca                                                                           <  4:716180/151‑1 (MQ=255)
                                                                         cTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggc                                                                            >  2:639186/1‑149 (MQ=255)
                                                                         cTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggc                                                                            >  4:1631094/1‑149 (MQ=255)
                                                                          tGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggcagg                                                                         >  1:1246543/1‑151 (MQ=255)
                                                                          tGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggcagg                                                                         >  7:360782/1‑151 (MQ=255)
                                                                           ggATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggcagg                                                                         <  6:742782/150‑1 (MQ=255)
                                                                             atatAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTg                                                                       >  8:764973/1‑150 (MQ=255)
                                                                             atatAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACAACCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTg                                                                       >  1:1496788/1‑150 (MQ=255)
                                                                                taACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATcc                                                                   >  6:1148159/1‑151 (MQ=255)
                                                                                taACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATcc                                                                   >  1:917738/1‑151 (MQ=255)
                                                                                   ccGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTGCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCaa                                                                <  1:760250/151‑1 (MQ=255)
                                                                                    cGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAAc                                                               <  8:1268025/151‑1 (MQ=255)
                                                                                      gTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGc                                                             >  7:177703/1‑151 (MQ=255)
                                                                                       tttCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGcc                                                            >  5:1256175/1‑151 (MQ=255)
                                                                                        ttCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCAAGGTAGTTCAGGCAGGTGATCCCAACGccc                                                           >  6:369106/1‑151 (MQ=255)
                                                                                         tCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCt                                                          >  8:472354/1‑151 (MQ=255)
                                                                                            gTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGAt                                                       <  6:1285641/151‑1 (MQ=255)
                                                                                               aTCACGCCTGG‑AGCCACTTCTCGCTGCACGGGAATGTTGGGTTCTAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATGCGGACAGCGTTGCCCGACCATGGTAGTTCAGGCAGGTGATCCCTACGCCCTGATTa                                                     <  3:191210/150‑1 (MQ=255)
                                                                                               aTCACGCCGGG‑AGCCACTTCTCGCTGCACCTGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTa                                                     <  1:1011589/150‑1 (MQ=255)
                                                                                                tCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAAt                                                   >  7:272310/1‑151 (MQ=255)
                                                                                                 cACGCCGGG‑AGCCACTTCTCGCTGCACGGGAATGTTGTGTTCAAGAGCCGTTTGTTCGAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCTTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAAt                                                   <  4:1457186/150‑1 (MQ=255)
                                                                                                 cACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATc                                                  <  8:1664793/151‑1 (MQ=255)
                                                                                                  aCGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATcc                                                 >  4:249751/1‑151 (MQ=255)
                                                                                                   cGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCGTGATTAATCCt                                                >  3:1448973/1‑151 (MQ=255)
                                                                                                   cGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCt                                                <  7:999584/151‑1 (MQ=255)
                                                                                                    gCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTg                                               >  2:1341472/1‑151 (MQ=255)
                                                                                                    gCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTg                                               >  7:1352567/1‑151 (MQ=255)
                                                                                                       tgg‑AGCCACTACTCGCTGCACCGGAATGTACTGTTCAAGAGCCCTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGAATAATCAGGCCAGCCTTCCCCGACCAAGGTAGTTCAGGCAGGAGCTCCCAACGACCTGATTAATACTGaa                                             >  2:895902/2‑149 (MQ=255)
                                                                                                             ccgcTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCgg                                        >  4:997236/4‑150 (MQ=255)
                                                                                                             ccACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGa                                       <  6:23891/151‑1 (MQ=255)
                                                                                                              cACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCGGTTTGTTCCAACATCCGTATCTGGCGTGGAGGGGTTATTAATCCGGCCAGCGTTCCCGGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGaa                                      <  1:1075384/151‑1 (MQ=255)
                                                                                                              cACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGAGCTCGGa                                       >  3:1236640/1‑150 (MQ=255)
                                                                                                               aCTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGaat                                     <  8:1524355/151‑1 (MQ=255)
                                                                                                               aCTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGaat                                     <  4:539729/151‑1 (MQ=255)
                                                                                                                  tctcGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGaata                                    <  2:815700/149‑1 (MQ=255)
                                                                                                                   ctcGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATc                                 >  3:164966/1‑151 (MQ=255)
                                                                                                                    tcGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCg                                >  3:1043427/1‑151 (MQ=255)
                                                                                                                     ggatggaCCGGAATGTTGTGTTCAAGAGGCGTTTTTTCCAACATCCGTATCTGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTGGGAATAATCgt                               <  4:82129/145‑1 (MQ=255)
                                                                                                                     cGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGaataat                                  <  1:237718/148‑1 (MQ=255)
                                                                                                                     cGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCgt                               >  1:475632/1‑151 (MQ=255)
                                                                                                                     cGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCg                                <  1:572774/150‑1 (MQ=255)
                                                                                                                        tGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCgtgt                             >  6:1359376/1‑150 (MQ=255)
                                                                                                                           aCCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAAt                         >  4:359383/1‑151 (MQ=255)
                                                                                                                            ccGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATc                        <  7:100897/151‑1 (MQ=255)
                                                                                                                                 aTGTTGTGTTCAAGAGCCGTTTGTTCCACCATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCACCGCCCTGATTAATCCTGACCTCGGAAAAATCGTGTAAATCAgggg                   >  4:4688/1‑151 (MQ=255)
                                                                                                                                   gttgtGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGgtg                 >  2:392733/1‑151 (MQ=255)
                                                                                                                                   gttgtGTTCAAGAGCCGTTTGTTCCAACATACGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGgt                  <  7:1472873/150‑1 (MQ=255)
                                                                                                                                     tgtgTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGgtgt                <  3:451153/150‑1 (MQ=255)
                                                                                                                                      gtgtTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTca              <  6:707501/151‑1 (MQ=255)
                                                                                                                                      gtgtTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTc               <  8:1418600/150‑1 (MQ=255)
                                                                                                                                        gtTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTcac             >  7:926789/1‑150 (MQ=255)
                                                                                                                                           cAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACg          >  1:1338539/1‑150 (MQ=255)
                                                                                                                                            aaGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGAt        <  7:1493474/151‑1 (MQ=255)
                                                                                                                                             agagCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGCCCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg       >  8:586170/1‑151 (MQ=255)
                                                                                                                                             agagCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATg       <  4:114707/151‑1 (MQ=255)
                                                                                                                                               agCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATgg      >  6:1654951/1‑150 (MQ=255)
                                                                                                                                                gCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAATTCAGGGGTGTCACACGATgg      >  3:1137125/1‑149 (MQ=255)
                                                                                                                                                gCCGTTTGTTCCAACATCCGTATCACGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGAAGATCAGCCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGCGGTATCACAAGAtcg      >  6:425632/1‑147 (MQ=255)
                                                                                                                                                 ccGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGt   <  2:1485504/151‑1 (MQ=255)
                                                                                                                                                  cGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTg  <  5:766864/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGG‑AGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTG  >  NC_000913/4530649‑4530944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A