Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 42 117.4 2145065 96.9% 2078567 261.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,310 A→C 10.6% L322V (TTA→GTA)  basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,3100AC10.6% 257.7 / 2.1 106L322V (TTA→GTA) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (47/43);  new base C (3/13);  total (50/56)
Fisher's exact test for biased strand distribution p-value = 1.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCG  >  NC_000913/4332040‑4332580
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                               
tCAGGCCAATTGAATCCTTAAGGTTCAATTAAACTCGGACGCGCATAATCTCCTCCAGGTTAAC‑GGAGGGGAGTG‑A‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAAccc                                                                                                                                                                                                                                                                              <  2:19961/274‑1 (MQ=255)
  aGGCCAATTTAACTCTTAAGGTTAACTTAATCGCGGACGGGCATAGTCTCCGCCGGGTTAAC‑GGGGGAGGGTG‑C‑AG‑TGCAAGACCGGGTGTATG‑AAAGTTTAGCTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑AGTGCTGGTTGGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGGGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAg                                                                                                                                                                                                                                                                           <  2:1026490/275‑1 (MQ=255)
    ccgaaTTGACGTCTTAAGTTTCACTTAATCTCCTACACGCTTACGCTCCTCCGGTTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCTTGCTGTTGTTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CCCTTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCTTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGAc                                                                                                                                                                                                                                                                         <  2:453168/272‑1 (MQ=255)
     ccAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTGCTGATCTTTCTTCAGCCATACCCAGGCCCGACTGCCGGACG‑TCTCTTGCCGGTTTTGCCGGAAAAACTGCCCGTGCTGCAACTGCGTAATGCGGCCGACCAAACTTCACCCCAGaac                                                                                                                                                                                                                                                                        >  2:233553/1‑273 (MQ=255)
     ccAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGCCAATACTTAACCCCAGAcc                                                                                                                                                                                                                                                                        >  1:252087/1‑275 (MQ=255)
                 ttAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTCATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGCTGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCAt                                                                                                                                                                                                                                                             >  1:39023/1‑274 (MQ=255)
                   aaagTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACACTACTTACACCCAGACCAATCCCGCCATAAc                                                                                                                                                                                                                                                          >  1:89717/4‑275 (MQ=255)
                   aaGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGCAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACCATCCTTAACCCCCGACCCATCCCGCCATAAc                                                                                                                                                                                                                                                          >  2:644386/1‑275 (MQ=255)
                     ggTTCACTTACCCCTGGACGGCCATACGCACCTCCGGTGTAAC‑GGGGGAGAGTG‑C‑AA‑TGAAAAACCGTTTTTATG‑AAGTTTTAACTACCTTGTACA‑GCGTGCTGGTGGTAGGCAGCTTTCTTT‑ATATCTTGTTGGC‑CCCGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGGGGGCCGGAGG‑TCTCTGGCCGGTTTTGAAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACgg                                                                                                                                                                                                                                                        <  2:39023/275‑1 (MQ=255)
                        tCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCt                                                                                                                                                                                                                                                      >  1:208376/1‑274 (MQ=255)
                         cACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCCTACCGGCTGt                                                                                                                                                                                                                                                    >  1:434255/1‑275 (MQ=255)
                                    tggtggacataccttcgtgaaGGTGAAC‑GGGGGAGA‑TG‑CAAA‑TTAAATGCCGTTTTTATG‑AAAGTTGAACTCCCTTGTCGA‑GCGTGCTGTTGTGCAGAACCTTTCTTT‑ATATCTGGTTTGC‑CACGTCTTATTCTTTCTTCAGCCGTACCCAGCCCCGCGTGCCGGAAT‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACa                                                                                                                                                                                                                                         <  2:922030/255‑1 (MQ=255)
                                     taCGCCCCTACGCTCCTCCGTGTTAGCGGGAGGAG‑GGG‑A‑AA‑GGAAAATCCGTGTTTAAG‑AAAGTTTTACTCCCGTTTTCA‑GCGTGCTGGTGTTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTTGTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATTCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACaa                                                                                                                                                                                                                                        <  2:215820/274‑1 (MQ=255)
                                      aCGCGCATAGCCTCCCCCAGTTTAAC‑GGAGGATATGG‑C‑AA‑TGAAAATCCTTTGTTATG‑AAAGTTTAACTCCCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTG‑ATATCTGGTTTGC‑CACGTACTGATCTTTTTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCGCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACaa                                                                                                                                                                                                                                        <  2:434255/274‑1 (MQ=255)
                                        ggacttcctctctcccgggttgaagggaggaGA‑TG‑A‑AA‑TAAAAAGCCGTGTTGATA‑CAATGTTAACTCCCTTGTTCA‑GCGGGCTGGTGGTCAGCACCTTTCTTG‑TTCGCTGGTTTGC‑AACGTCCTGATCTTCCTCCAGCTGTCCCCCGGCCCGCGTGCCGGAAG‑TCTCTTGCCGTTTTGACAGGAAAAACTGCCCGCGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCACTCCCGCCATAACGGCTGTCCATACGTACAAACg                                                                                                                                                                                                                                     <  2:1059222/250‑1 (MQ=255)
                                          tcattcgctcctcGCGGTTAA‑‑GGGGGGGTGTG‑C‑GATTGAAAAACCGTGTTTTCG‑AAGTTTTAACACCGGTGTTAA‑GAGTCCTGGGGTTCAGCGGTTTTCTTT‑ATTGCGTGTTGAC‑CACGTTCTTATCTTTCTTCAGCCGTACCCGGGCCCGCGTGCCGGAAG‑TCTCTTTCCGTTTTTCCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCt                                                                                                                                                                                                                                   <  2:449324/268‑1 (MQ=255)
                                          gcATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAACCCGTGTTTATG‑AAAGTTTACCTACCGTGTTCA‑GCGTGCTGGTGTTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCt                                                                                                                                                                                                                                   <  1:689117/275‑1 (MQ=255)
                                          gcATACTCTCCTCCAGGTGAAG‑TGGGGGGAGGT‑A‑AG‑TCAAAAACCGTTTTTATG‑AACG‑TTAACTGTCGGTTTCACGCGTGTGGGTGGTCGCCGACTTTCTTG‑ATATCTGGTTGGA‑CCCGTACTGTTCTTTTTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCt                                                                                                                                                                                                                                   <  2:463386/275‑1 (MQ=255)
                                           cATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACGTAACCCCCGACCAATCCCGCCATAACGGCGTGCCATACGTACAAACGCt                                                                                                                                                                                                                                   >  2:118734/1‑274 (MQ=255)
                                                   tctccAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAAACGTGTTTATG‑AAAGTTTAAGTACGGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTATTT‑ATATATGGTTTGC‑GAAGTACTGATCTTTGTTCAGCAGTACCTAGGCCCGCGTGCGGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCGGTGATGCAAATGTGAAATGCGGATGATAATAGTTAACGCAAGAGGAATCCAGCAATAACGGTTGTCGGTACGTGAAAAGGCTTTatataaa                                                                                                                                                                                                                          >  2:312154/2‑269 (MQ=255)
                                                    ctccAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAACACTGCCCGTGCTGCAACTGCGTAATGCGGCTGACAATACTTAACCCAAGACCAATCCCGCCATAACGGCTGTCCATCCGTAAAAACGCTTTAACCAAc                                                                                                                                                                                                                          >  2:704484/1‑274 (MQ=255)
                                                       cAGGTTAAC‑GGAGG‑GAGGGTC‑AA‑TGAAAAACCTTTTTTATG‑AAAGTTGAACTACCGTGTTCA‑GCGTGCTGGTGTTCAGAAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAT‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGAAACTGTGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                                                      <  1:861226/275‑1 (MQ=255)
                                                        cggTTAACGGGAGGAG‑GT‑‑C‑AA‑TGTAAAACCGTGTTTATGTAAAGTTTAGCTCCCGTGTTCG‑CGGTGTGGGTGTTCAGCAGCTTTTTTT‑TTATCGGGTTGTC‑ACCGTCCTGATCTTTCTTCAGCC‑‑CCCAAGGCCCGGGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                                                      <  2:422522/271‑1 (MQ=255)
                                                          gTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGc                                                                                                                                                                                                                   >  1:290613/1‑275 (MQ=255)
                                                              aC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGACAAACTGCCCGTGATGCAACCTTGTACTGCGGCTGACAATCCTTCACCCAAACACAATACCGCCATAACGGCTGTCCCTCCGTACACAAGCTTTAATCAACTCCCCCCAtt                                                                                                                                                                                                                >  2:1045586/1‑274 (MQ=255)
                                                              aC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTAAACTCCCCGCAtt                                                                                                                                                                                                                >  1:399303/1‑274 (MQ=255)
                                                              aC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACCCAACTCCCCGCAtt                                                                                                                                                                                                                >  1:684785/1‑274 (MQ=255)
                                                                  acagagaGTT‑C‑AA‑TGAAAAACCGTTTTTTTG‑AAAGTTTACCTCCCGTTTTCA‑CCGTGCTGTTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CCCGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTTTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑Act                                                                                                                                                                                                           <  1:997168/272‑1 (MQ=255)
                                                                     gggggggggtaaaggAAAATCCGGGTTTATG‑AAAGTTTACTTTCCGTGTTCA‑GCGTGTTGGTGGTCCGCAGTTTTTTTT‑ATATCTGGTTTGC‑CACTTACTGTTCTTTCTTCAGCCGAACCCAGGCCGGGGGGCCGGAGG‑TCTCTGGCCGGTTGTGCAGGAAAAGCTGCCCGTGATGAAACTGGGTAAGGGGGCTAACAATCCTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑Act                                                                                                                                                                                                           <  2:1069057/261‑1 (MQ=255)
                                                                     gagaGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTACTGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAAAGGCTGTCCATACGTACAAACGCTTTCCTCCACTCCCCCCCTCT‑Actcc                                                                                                                                                                                                         >  2:797381/1‑273 (MQ=255)
                                                                     gagaGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCAATAACGGCTTTCCATACGTACAAACGCTTTACCCACCTCCCCGCACTT‑Actcc                                                                                                                                                                                                         >  1:1029863/1‑273 (MQ=255)
                                                                     cgggggggtcaaatagaaagctgtctttGTG‑AAAATTTACCGCCCGTGTTCA‑TCGGTAGGTTGGTCATCAGCTTTCTTT‑‑TATTTGGGTTGCGCACTGAGTGTGTTTTTTTCAGCGGTACCTGCCCCGGCTTGCGAGAAG‑TCTCTCGCCGGTTTTGGAGGAAAAACTGCCCGTGATGCAACTGTTTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑Act                                                                                                                                                                                                           <  2:14627/250‑1 (MQ=255)
                                                                          tG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTAATCAACTCCCCGCATTT‑ACTctcctc                                                                                                                                                                                                     >  1:278070/1‑273 (MQ=255)
                                                                           gttgtacggAAAACACGTGTTAATG‑AAACTTTACCTACCGTGTTCA‑GCGTGCTGGGGGTCGGCAGCTTTCTTT‑ATATGTGTGTTCC‑AACGGACTGATCGTTCTTAAGGCGAACCCAGGCCCGCGGGCCGGAAG‑TCTCTTGCCGGTTGTGCAGCAAAAACTGCCCGGCATGCAACTGTGTAGTGCGGCTGACAATACTGACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCtcatc                                                                                                                                                                                                     <  2:462914/267‑1 (MQ=255)
                                                                                  ggagaaaGCGTGTGTAAG‑AAAGTTGATCAACGGTGTTAA‑GCGGGCGTGTGGTCAGCAGTTTTATTT‑ATATCTGGTATGC‑AACATACTGTTCGTACTAAAGCCGAACCAAGGCCAGCGTGACGGAAG‑GCTTTTGCCGTTTTTGAAGGAAAAACTGCCCGTGATGCAACTGGGAAAGGGGGCTGACAATACTAAACCCCAGACAAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATAcc                                                                                                                                                                                               <  2:706322/270‑1 (MQ=255)
                                                                                     aaaaCCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTg                                                                                                                                                                                             >  1:405354/1‑273 (MQ=255)
                                                                                       aaCCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATCCCTGGTc                                                                                                                                                                                          >  1:761376/1‑274 (MQ=255)
                                                                                            tgtTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCACCTCCCAGCATTT‑CCTCTCATCAACACCTGGTCCTTCAt                                                                                                                                                                                    >  1:1048945/1‑275 (MQ=255)
                                                                                              tttATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGGTTAATGCGGCTGACAATACTTAACCACAGACCAATCCCGCCATAACGGCTTTCCATACGTCCAAACGGTTTACTAAAATCCCCGCCTTT‑ACCGTCAACAATACCTTGTCCTTCATc                                                                                                                                                                                   >  1:4568/1‑274 (MQ=255)
                                                                                                 aTG‑AAATTTTACCTACCTTGTTCA‑GCTTTCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTTTTCTTTCTTCAGCCGTACCCAGGCCCGCGGGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCATACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCAGCAATACCTGGTCCTTCATCTTCa                                                                                                                                                                               <  1:797381/275‑1 (MQ=255)
                                                                                                  tG‑AAATTTTAACTACCGTGTTCA‑GCGGGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTTGTTTGC‑CACGTACTGATCTTTCTTCCGCCGTACCCAGGCCCGCGGGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGAAAATACTTAACCCCATCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCaa                                                                                                                                                                              <  1:393612/275‑1 (MQ=255)
                                                                                                       aGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCCTTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTg                                                                                                                                                                           >  2:259570/1‑274 (MQ=255)
                                                                                                                  cGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATCCGTACAAACGCTTTACTCAAATCCCCGCATTTCACTC‑CATCCATCCCTGGTCCTTCACTCTCAACTGCCATGACCGcc                                                                                                                                                                >  2:981575/1‑273 (MQ=255)
                                                                                                                         a‑GCGTGCTGTGGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGTTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGAAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCAtc                                                                                                                                                        <  1:644386/275‑1 (MQ=255)
                                                                                                                         a‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTAAACTCACCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCTGCAtc                                                                                                                                                        >  1:480322/1‑275 (MQ=255)
                                                                                                                                cTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCCTTACTCAACTCCCCGCATCT‑ACTCGCATCAATACCTGGTCCTTCCTCTTCAACTGCCATGACCCGTCCGTCATCCTCTTg                                                                                                                                                  >  1:455511/1‑275 (MQ=255)
                                                                                                                                    tggtgcagagcTTTCTTTGAGTTCTTGTTTGC‑AAGGTGTTGTTCTTGCTGCGGCCGTACCCGGGCCGGGGTGCCGGACG‑TGTTTCGCCGTTTTTGCAGAAAAAACAGCCTGTGAGGCAACTTGGTAAGGCGGCTGACAATACTTAACGCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAg                                                                                                                                               <  2:680094/267‑1 (MQ=255)
                                                                                                                                    tggtCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAg                                                                                                                                               <  1:97463/274‑1 (MQ=255)
                                                                                                                                      gtCAGCAGCTTTCTTT‑ATTTCTGGTTTGC‑CACGTACGGCTCTTTCTGCACCCGTACCCAGGCCCGGGTGCCGGAAG‑TCTCTTGCCGGTTGTGCAGGAAAAACTGCCCGGTATGCAACGGTGTAATGCGGCTGAAAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCtt                                                                                                                                            <  2:910910/275‑1 (MQ=255)
                                                                                                                                           cagcTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCCCCGTCATCTTCTTGCAGCTTAATCa                                                                                                                                       >  1:293550/1‑275 (MQ=255)
                                                                                                                                           cagcTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACCGCCATGACCGCCCCGCCATCTTCTTGCAGCTTAATCa                                                                                                                                       >  2:377338/1‑275 (MQ=255)
                                                                                                                                                ttcttt‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATACCGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCACTACCTGGTCCTTCATCTTCAACTGCCATGACCGCCCCGTCATCTTCTTGCAGCTTAATCATAATg                                                                                                                                  >  1:829520/1‑275 (MQ=255)
                                                                                                                                                     gttgttcgggTTTGC‑GACTGACTGTTCTGTTGCCAGCCGTACCCGCGCCCGCGTGCGGGAAG‑TCGCTTGCCGGTTTGGCAGAAAAATCTCCCCGGAATGCAACTGTGTAAGGCGGTTGACAATACTTAACCCCAGCCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtg                                                                                                                               <  2:684785/266‑1 (MQ=255)
                                                                                                                                                       atcgatGGTTTGC‑CACGTTCTGCTCTTGATACAGCCTGACCCGGGCCCGGGTGCCGGAAT‑GCTCGGCCCGGTTTTGAAGGAAAACCTGCCCGTGATGCAACGCGGGAAGGCGGCTGAAAATACTAAACCCCAGACCAATCCCGCCATAACGGCTTTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTtgct                                                                                                                             <  2:580250/269‑1 (MQ=255)
                                                                                                                                                            tGGTTTGC‑CACGTCCTGATCTTTCTTCCGCCGTACCCAGGCCCGCGGGCCGGAAG‑TCTTTTGCCGGTTTTCAAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGTGGACAATACTTAACCCCAGACCATTCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTg                                                                                                                       <  1:981576/275‑1 (MQ=255)
                                                                                                                                                             ggggtgaccgcgtaccggtggttctccGGCCGCACCCGGGCCGGCGTGCCGTAAG‑TCTGTGCCCGTTTTTGCCGGAAAACCTCCCCGTGTTGCAACTGTGTACGGCGGCTGACATTACTGAACCCCATACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACCCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTg                                                                                                                       <  2:4568/254‑1 (MQ=255)
                                                                                                                                                              gTTTGC‑CACTGACTGATCTTTCTCCACCCTTACCCAGCCCCGCGTGCCGGAAG‑TCGCTGGCCGGTGTTGCAGGAAAAACTGCCCGTGATGCAACGGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                     <  1:268281/275‑1 (MQ=255)
                                                                                                                                                              gTTTGC‑CACGTCTGGTTCTTTCTTACGCCGTACCGAGGCCCGCGTGCCGGAAG‑TCCCTTGCCGGTTTTGCAGAAAAACCTGCCCGGAATCAAACTTGTTAATGCGGCTGACAATCCTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                     <  2:399303/275‑1 (MQ=255)
                                                                                                                                                              gTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGTCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCCTTTTCTTGCAGCTTAATCCTAATGTTTCTGCCtttcc                                                                                                                     >  1:726430/1‑272 (MQ=255)
                                                                                                                                                               ttttgccagtgcttgactttcctccacccGTACCCAGGCCCGCTGGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                     <  1:143348/248‑1 (MQ=255)
                                                                                                                                                                          aCTTATCTTTCTTCCCCCTTACCAGGGCCCCCGTGCCGGAAG‑TGTCTGCCCGTTTTTCCAGGAAAAACGCCCCGTGATGCAACTGTGTAAGGCGCCTGACAATCCTTACCCCCAGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGa                                                                                                          <  2:789627/275‑1 (MQ=255)
                                                                                                                                                                              aTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCCCTACGTCATCTTCTTGCAGCTTAATCATAATGTTCATGCCTTGCGGGCTGTAACGAgggg                                                                                                      >  1:970150/1‑275 (MQ=255)
                                                                                                                                                                                  tttttcgcccGTACCCAGGCCCGCGTGCCGTATG‑TCTCTTGCCGGTTTTGAAGGAAAAACTTCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGtt                                                                                                  <  1:348742/267‑1 (MQ=255)
                                                                                                                                                                                  ttcctcgcccgaacccgtcccccggTGCCGGAAT‑TCTCTTCCCGGTTTGGCAGGAAAAACCTCCCGTGATGCAACTGTGTAATGCGGCTCACAATACCTAACCCCAGACCACTCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGtt                                                                                                  <  2:208376/251‑1 (MQ=255)
                                                                                                                                                                                   tcttcAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCCTCTTCTTGCAGCTTAATAATAATGTTTCTGCCTTTCGGGCTGTAACGATGGGCgct                                                                                                  >  1:406382/1‑272 (MQ=255)
                                                                                                                                                                                   tcttcAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATTTTGCTGCCTTCCTGGCTGTAACGATGGGCGtt                                                                                                  >  1:942119/1‑274 (MQ=255)
                                                                                                                                                                                   tcttcAGCCGTACCCACGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGCAAAACTGCCCGTGATGCACCTGTGTAACTCGGCTGACAAGACTTACCCACCGACCAATCCCCCCATAAAGGCTGTCCATAAGTCCACACGCTACACACAACACCCCGCATTT‑CCTCTCAACAATACCGTGTACTTCATCCTCCACGGCCATGACCGCTCCGTCATCTTCTTTCCGCTTATGCTTACTGTTCCCTCCTTGCGTGCtcatacgtacggcactc                                                                                                 >  2:125258/1‑259 (MQ=255)
                                                                                                                                                                                   tctccAGCGGTACCCGGGCCCGCGTGCAGAAGG‑GCTCTTGCCGGTTTTGCAGGAAAAACGCCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGtt                                                                                                  <  1:954844/274‑1 (MQ=255)
                                                                                                                                                                                    tctcACCCGGACCCAGGCCCGGAGGCGTGAAG‑TCTCTTGCCGGTTTGGAAGGAAAACCTCCCCGTGATGCAACGGTGTAAGGCGGCTGAAAATACTTAACCCCAGACCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGtttt                                                                                                <  2:505312/273‑1 (MQ=255)
                                                                                                                                                                                        aGCCGTACCCAGGCCCGCGTTCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATCCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCCTTT‑ACTCTCATCACTCCCTGGTCCTTCATCTTCCACTGCCATGACCCCTCCGTCCTCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTACCGCTGGGCGTTTTCTAc                                                                                            >  2:758842/1‑275 (MQ=255)
                                                                                                                                                                                            ctccccgccccggcgAGCCGACAG‑TGTCTTGGCGGTTTGACAGAAACAACCGCCCGTGATACAACTGGTTAATGCTGCGTAAAATCCTTACCCCCACCCCATCTCCTCCATAACCGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAgg                                                                                        <  2:726430/263‑1 (MQ=255)
                                                                                                                                                                                                    gCCCGGGTGTCCGAAGTTCTCTTCCCGTTTTGGAAGGAAAAACTGCCTGTGATGAAACTGTGTAATGCGGCTAACAATCCTTAACCCCAGACCATCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCa                                                                                 <  2:761376/275‑1 (MQ=255)
                                                                                                                                                                                                         cgTGCCGGAAG‑TCTCTTGCCGGTTTTGCCGGAAACACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACCCCCCGCATTT‑ACTCTCCTCAATACCTGGTCCTTCATCTTCAACTGCCCTGACCGCTCCGTCATCTTCTTGCAGCTTAATCTTAATGTTGCTGCCTTGCGGGCTGTCACGATGGGCGTTTTCTACCCGGTTTCGCAATagcc                                                                           >  2:550666/1‑272 (MQ=255)
                                                                                                                                                                                                         cgTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCCTAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCCTCT‑ACTCTCATCAATACCTTGTCCTTCCTCCTCAACTGCCATCAACGCTCCGTCATCTTCTTCCCGCTTAATCATAATGTTGCTGCCTTGCGGGCGTTAACGCTGGGCGTTTTCCACCCGGTTTCGCAAttaaa                                                                           >  2:281251/1‑271 (MQ=255)
                                                                                                                                                                                                         cgTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTAAAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCCCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCACGTTTCGCAATAACa                                                                           >  1:416261/1‑275 (MQ=255)
                                                                                                                                                                                                             ccGGAAT‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGAAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTg                                                                                                                       <  1:151259/227‑1 (MQ=255)
                                                                                                                                                                                                                    gtttctttcggtgtttGCAGGAAAAACTGCCCGGGATGCAACTGTGTAATGCGGCTAAAAATACTTAACCCCAGACCCATCCCGCCATAACGGCTTTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCgcag                                                                    <  2:1029862/259‑1 (MQ=255)
                                                                                                                                                                                                                      cttctGCCGGTTTTACAGGGAAAGAGGCCCGCGATGCAACTGTGAAATGCGCCACACATAACTGAACCCCCGACCAGGCCCGCCATAGCGCCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCGTCATCTTCAACTGCCGTGACCGGTCCGGCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAggg                                                               <  2:455511/271‑1 (MQ=255)
                                                                                                                                                                                                                      ctctGGCTGGTGGTGCAGGAAAAACTGCCCGTGAGGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGGCCTAGCCCGCCATAACGGCTTTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGGCCGCTCCGTCATCTTCTTGCAGCGTAATCAGAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAggg                                                               <  1:451960/275‑1 (MQ=255)
                                                                                                                                                                                                                         ttGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCCGGGTTg                                                            >  1:283048/1‑275 (MQ=255)
                                                                                                                                                                                                                            cgggTTTGGCAGAAAAAACGGCCCTGGATGAAATTGTGTAATGCGGCTGACAATCCTTACCCCCAGCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                          <  1:281251/272‑1 (MQ=255)
                                                                                                                                                                                                                            ccGGTTTGGCAGGAAAAACTGCCCTGGATGCAACTGTGTAAGGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                          <  1:377338/274‑1 (MQ=255)
                                                                                                                                                                                                                            ccGGTTGTGCAGAAAAAACTGCCCGGGTGGCAACCGTGTAATGCGGCTGACAATACTTACCCCCAGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                          <  2:406382/274‑1 (MQ=255)
                                                                                                                                                                                                                                ttttacaggaaacagccccccgtattcAACTGTGCACGGCGGCTCACAATACTTACCCCCACACCCATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAcc                                                     <  2:829519/249‑1 (MQ=255)
                                                                                                                                                                                                                                ttttGCAGGAAAAACGGCCCGTGAGGAAACTGTGTAATGGCGCAGAAAATACTTAACCCCAGACAAATCCCGCCATAACGGCGGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAcc                                                     <  1:550666/275‑1 (MQ=255)
                                                                                                                                                                                                                                   tggaGGAAAAACCGCCCCGGATGCAATTGTGTGATGCGGCTTAAATGTCTTAACCCCGGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCCCATTT‑ACTCTCATCAATACCTGGTCCTTCAACTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTg                                                  <  2:252087/272‑1 (MQ=255)
                                                                                                                                                                                                                                   ggCAGGAAAAACACCCCGGGATGCAAGTGTGGAAGGCGGCTACCAATACTTACCCCCAGCCCAATCCCGCCATAACGGCTGTCCATACTTCCAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTg                                                  <  2:170668/274‑1 (MQ=255)
                                                                                                                                                                                                                                    ccAGGACAAACTCCCCGTGATGCACCTTTTTAATCCGACTAACAATACTAAACCCCCAACCTATCCCGCCATAACGTCTGTCCATACGTACAAACGCTTTACTCAACTCCCCTCATTT‑ACTCTCATCAACACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGa                                                 <  1:312154/274‑1 (MQ=255)
                                                                                                                                                                                                                                                gCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTTCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTc                                      >  1:375688/1‑274 (MQ=255)
                                                                                                                                                                                                                                                   cGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGCTGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggcg                                  >  1:465322/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       aTGCAACTGTGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAAGGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGgcggcg                               <  1:1045587/274‑1 (MQ=255)
                                                                                                                                                                                                                                                          cgcaaaatttgtataagggACAGTACTTAACCCCAACCCAACCCCGCCATACCGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctct                           <  2:340812/257‑1 (MQ=255)
                                                                                                                                                                                                                                                                tgtAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTgg                       >  1:14916/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                tgtAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCCCTCTgg                       >  1:332896/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                tgtAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGCTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCTCTCTCTgg                       >  2:265310/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                     tggggCTGACAATACTTACCCCCAGACCAGTCCCGCCATAACGGCTGTCCATACGTACAAACGCGTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                 <  2:871862/271‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTTAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAggg            >  1:369069/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                          cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTGTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTAACCGGTGATGGCGGCGGCGCCCTCTggacataccgtgg            >  2:798612/1‑263 (MQ=255)
                                                                                                                                                                                                                                                                          cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTCCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCCGAACGGTGATGTCGGCGGCGCTCTCTGGCAATGGCAggg            >  1:468556/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                   cTTAACCCCAGACCACCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCg  <  1:840662/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                               
TCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAAC‑GGAGGAGAGTG‑C‑AA‑TGAAAAACCGTGTTTATG‑AAAGTTTAACTACCGTGTTCA‑GCGTGCTGGTGGTCAGCAGCTTTCTTT‑ATATCTGGTTTGC‑CACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAG‑TCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTT‑ACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCG  >  NC_000913/4332040‑4332580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A