Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F3 I0 R1 49 96.5 1638068 98.5% 1613496 272.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,310 A→C 10.7% L322V (TTA→GTA)  basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,3100AC10.7% 319.0 / 5.7 116L322V (TTA→GTA) basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base A (60/40);  new base C (2/13);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 1.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGT  >  NC_000913/4332023‑4332595
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             
gATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAAc                                                                                                                                                                                                                                                                                              <  2:545190/289‑1 (MQ=255)
           gTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCaa                                                                                                                                                                                                                                                                                    >  2:148436/1‑288 (MQ=255)
           gTCTTATAAGGCAAATTAAACTCTTAAGGTCCAGTAAATCTCGGACGCGCATCCTCTCCCCCAGGTTAACGGAGGAGGTT‑GCAATGAAAAACCGTTTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTGCTTCAGCCGTACCCAGGCCCGCGGGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAAt                                                                                                                                                                                                                                                                                   <  1:443109/289‑1 (MQ=255)
            tCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAAt                                                                                                                                                                                                                                                                                   >  2:348826/1‑288 (MQ=255)
              ttATCAGGCCAATTGAACTCTTAAGGTTCACTTAAGCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GAAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATccc                                                                                                                                                                                                                                                                                <  2:464411/289‑1 (MQ=255)
                    ggCCAATTGAACTCTTAAGGTCCACTAAATCCCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GAAATAAAAAACTGTTTTTATGAAAGTTTAACTACCGGGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGTTTTGCCACGTACTGCTCTTTCTTCAGCCGGACCCAGGCCCGCGGTCGGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCAt                                                                                                                                                                                                                                                                           <  1:148436/288‑1 (MQ=255)
                      ccAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCATCCGTACCCAGGCCCGCCTGCCGGAAGTCTATTGCCGGTTTTGCAGGCAAAACTGCACGTGATGCAACTGTTTAATGCGGCTGCCAATACTTAACCCCCGACCAATCCCGCAAtca                                                                                                                                                                                                                                                                         >  1:392909/1‑286 (MQ=255)
                      ccAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTATTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATaaa                                                                                                                                                                                                                                                                        >  2:112123/1‑288 (MQ=255)
                        aaTTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACg                                                                                                                                                                                                                                                                       >  2:253280/1‑288 (MQ=255)
                           tgaaccttgaaGTTTCATTAAATCTCTGACGCGAATACTCTCCTCCAGGTTAACGGGGGAGAGT‑GAAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTg                                                                                                                                                                                                                                                                   <  1:113662/280‑1 (MQ=255)
                                    aaGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCTTACCCAGGCCCGCGTGCCGGACGTCTCTTGCCGGTTTTGCAGGACAACCTGCCCGTGATGCAACTGTTTAATTCGGCTTGAATTCCTTATCCCCCTACAAAACACGCCCTTACGTCTGCCCATACGt                                                                                                                                                                                                                                                          >  1:734070/1‑289 (MQ=255)
                                          cACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATTCAACTGGGTAATGCGGCTGACAATACTTAACCCCAGACCAATCACGCCATAACGGCTTTCCATACGTACAAAc                                                                                                                                                                                                                                                    >  1:683501/1‑289 (MQ=255)
                                          cACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAAc                                                                                                                                                                                                                                                    >  2:441256/1‑289 (MQ=255)
                                          cACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAAc                                                                                                                                                                                                                                                    >  2:192113/1‑289 (MQ=255)
                                            cTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTAAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACCAACg                                                                                                                                                                                                                                                   >  2:46604/1‑288 (MQ=255)
                                                  ctctGACGCGCATACTCTCCTCCACGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTCAACTACCGTGTTCCGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCTCGTTCCGGAAGTCTCTTTCCGGTTTTGCAGGCAACACTGCCCGCGCTGCACCTGTGTAATGCGGCTCACACTTCTTACCCCCAGCACAATCCCGGCATCATGGCCCGCTATACGTTCCACCGCtttcc                                                                                                                                                                                                                                             >  1:619883/1‑286 (MQ=255)
                                                     tGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACTAACAAACCCTTTACCCac                                                                                                                                                                                                                                         >  1:425245/1‑288 (MQ=255)
                                                     tGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCa                                                                                                                                                                                                                                          >  2:704078/1‑288 (MQ=255)
                                                           gcATAATCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                                                                    <  2:643338/288‑1 (MQ=255)
                                                            cATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGACAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCAGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACACACGCTTTACCCAACTcccc                                                                                                                                                                                                                                   >  1:267610/1‑288 (MQ=255)
                                                               acgtcccacagggTTAAGGGGGGGAAGT‑GAAATGAAAACCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCTGGCTGGTGGTCGGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCAt                                                                                                                                                                                                                               <  1:192113/278‑1 (MQ=255)
                                                                  cacctccAGGTAAACGGGGGAGGGG‑GAAATGAAAAACCGTGTTTATGAAATTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCGGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGAAAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTa                                                                                                                                                                                                                            <  1:148317/287‑1 (MQ=255)
                                                                    cctccAGGTTAGCGAGGGAGAGT‑GAAATGAAAAACCGTTTTTATGAACGTTTACCTACCTTGTCCAGCTTCCGGGTGTTCAGCGCCTTTCGTTAGATCTGGTTTGCACCGGACTGATCTTTCTTCAGCCTTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACGGCCCGTGATGCAACTGTGTAAGGCGGCTGAACATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTAct                                                                                                                                                                                                                          <  1:441256/289‑1 (MQ=255)
                                                                     cgccAGGTTAGAGGAGGAGAGG‑ACAATGAAAAACCGGGTTTATGAAGGTTTAACGACCGTTTTCCGCGTGCTGGTGCTCAGCACCTTTCTTTAAATCTGGTTTGCCTCGTACGTATCTTTCTTAACACCACCCCAGGCCCGCGTGCCGGACTTCCCTTGCTGGTTTGCCAGAAAAACCTGCCCGTGACGCAACTGTTTAAGGCGGCTGACAATACTTACCCCCTGCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTActc                                                                                                                                                                                                                         <  1:40729/287‑1 (MQ=255)
                                                                     atcaaGGTTAACGG‑GGGGAGTGGAAATGAAAAACCTTGTTTTTGAAATTTTAACTACCGTGTCCAGCGTTCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTActc                                                                                                                                                                                                                         <  2:350423/285‑1 (MQ=255)
                                                                      cccAGGTTAACGGTGGAGGGG‑GAAATGAAAAACCGTGTTTATGAAAGTTAAACTACCTTGTTCAGCGTGCTGTTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCCCTTGCCGGTTTTGCAGGAAAACCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCATTCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTActct                                                                                                                                                                                                                        <  1:708423/288‑1 (MQ=255)
                                                                          ggTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCtcatc                                                                                                                                                                                                                    >  2:449203/1‑289 (MQ=255)
                                                                           gTTAACGGAGGGGAGT‑GCAATGAAAAACCGGGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACGGATCTTTCTTCAGCCGTACCCAGCACCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGCCCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCtcatc                                                                                                                                                                                                                    <  2:814173/288‑1 (MQ=255)
                                                                            tgaaCGGAGGGGCGT‑GCAAAGCAAACACGTTTTGATGAAAGTGTAACTTCCGTGTTCACTGGGCTTGGTGTCAGCAGCTTTGTTTATATTTGGTTTTCCAAGCACGGATTTTTCTTAGGCCGTACCCGGGCCCAAGTGCCAGAAGTCTTGTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGGTATGCGGCTGACCAAACTTAACCCCAGACCCATCCCGGCATAACCGCCGGCCCTACCTACAAACGCTTTACGCAACTCCCCGCAATTACTCtcatca                                                                                                                                                                                                                   <  1:675848/286‑1 (MQ=255)
                                                                                        agT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCt                                                                                                                                                                                                       >  2:565403/1‑288 (MQ=255)
                                                                                          t‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATTTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGTAGTCTCTTGCCGGGTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATCCGGCTGACAATACTTAACCCCAGGCCAATCCCGCCATTACGGCTGTCCATACGTAAAACCGCTTTACTCAACTCCCACCATTAACTCTCATCAATACCTGGTCCTTCa                                                                                                                                                                                                    >  2:352133/1‑289 (MQ=255)
                                                                                          t‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTAct                                                                                                                                                                                                                          >  2:436288/1‑267 (MQ=255)
                                                                                          t‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCa                                                                                                                                                                                                    >  2:388322/1‑289 (MQ=255)
                                                                                          t‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCACACATACCTGGTCCtt                                                                                                                                                                                                      >  1:693838/1‑287 (MQ=255)
                                                                                            gaaaTGAAAACCCTTGTTTATTAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATAGCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCaa                                                                                                                                                                                                   <  2:638735/287‑2 (MQ=255)
                                                                                                tGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTTTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTAATCAATCCCTGCTCATTAATCTTc                                                                                                                                                                                               >  2:224934/1‑289 (MQ=255)
                                                                                                tGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCCTCTTc                                                                                                                                                                                               >  2:93469/1‑289 (MQ=255)
                                                                                                tGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTc                                                                                                                                                                                               >  2:772310/1‑289 (MQ=255)
                                                                                                   aaaaCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGTAAAACTGCCCGTTATGCAACTGTGTAATGCGGCTGACAATACTTAACCCAAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATATACTCTCATCAATACCTGGTCCTTCATCTTCa                                                                                                                                                                                              >  2:280820/1‑287 (MQ=255)
                                                                                                   aaaaCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCACCGCATTTACTCTCATCAATACCTGGTACTTCATCTTCa                                                                                                                                                                                              >  2:203752/1‑287 (MQ=255)
                                                                                                   aaaaCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTCTACTCAACTGCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCa                                                                                                                                                                                              >  2:45310/1‑287 (MQ=255)
                                                                                                   aaaaCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCAAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCGCATCAATACCTGGCCCTTCATCTTcca                                                                                                                                                                                             >  1:258993/1‑286 (MQ=255)
                                                                                                     aaCCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACt                                                                                                                                                                                           >  2:622277/1‑288 (MQ=255)
                                                                                                          tgtTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATg                                                                                                                                                                                     >  1:608227/1‑289 (MQ=255)
                                                                                                                              cctggttcctcgtgtgggtgttCACCCGTTTTCTTTATTTCTGTTTTGCCCCTTCTTGTTTTTTTTGCAGCCTAACCCGGGCCCGAGTCCCGTTAGGTCCTGGCCGGTTTTGCAGAAACACCTCCCGGTGATCCAACTGTGTAATGCGGCTTACAATACTCAACCCCGGCCCAGACCCGCCATAACTGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGTTCCTTCATCTTCCACTGCCATGACCGCTCCGTCATCTTCTTg                                                                                                                                                                 <  1:352133/273‑1 (MQ=255)
                                                                                                                              ccGTGTTCAGCGTGTTGGTGTTCGCCAGTTTTTTTTATATCTGGTTTGCCACGTACTGCTCTTTCTTCACCCGTACCCAGCCCCGCGTGCCGAAAGTCTCTTGCCGGTTTTGCAGAAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCt                                                                                                                                                                               <  1:687555/275‑1 (MQ=255)
                                                                                                                               cGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCt                                                                                                                                                                               >  1:485358/1‑274 (MQ=255)
                                                                                                                                gtgtTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCa                                                                                                                                                               >  2:731234/1‑289 (MQ=255)
                                                                                                                                gtgtTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCa                                                                                                                                                               >  2:728183/1‑289 (MQ=255)
                                                                                                                                   ttCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTTACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCt                                                                                                                                                            <  2:457726/289‑1 (MQ=255)
                                                                                                                                   ttCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCt                                                                                                                                                            >  1:511948/1‑289 (MQ=255)
                                                                                                                                      aGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCCACTGCCAATACCCCTCCGTCATCTTCTTGCAGCTTaa                                                                                                                                                         >  1:799523/1‑289 (MQ=255)
                                                                                                                                        tgTGCTGGTGGTCAGCAGCTTTTTTTTTTTCTGGTTTGCCCCGTACTGTTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAAGGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAAt                                                                                                                                                        <  1:772310/287‑1 (MQ=255)
                                                                                                                                        cGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                                                                    >  2:17222/1‑212 (MQ=255)
                                                                                                                                        cGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTccc                                                                                                                                                                                                                                    <  1:17222/212‑1 (MQ=255)
                                                                                                                                        cGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCCtctt                                                                                                                                                                     >  2:687555/1‑275 (MQ=255)
                                                                                                                                          tGCTGGTGGTAAGCAGCTTTCTTTATTTCTGGTTTGCCCCGTCCTGTTCTTCCTTCAGCCGTCCCCAGGCCCGGGTTCCGGAGGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCACCTGTGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCAt                                                                                                                                                     <  2:799523/289‑1 (MQ=255)
                                                                                                                                           gCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATa                                                                                                                                                    >  1:251846/1‑289 (MQ=255)
                                                                                                                                                           tttctttATTGTTGGTTTCCCACGTCCGGTGCTTGTTCCAGCCGTACCCAGCCCCGCGGGCCGTAAGTTTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTAATGCAACTGTTTAATCCGGCTGACAATCCTTACCCCCAGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                                    <  1:731234/289‑1 (MQ=255)
                                                                                                                                                           tttctttAGATCTGGTTTGCCACGACCTGTTCTTTCTTCACCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTGGCAGGAAAAACTCCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                                    <  2:441233/289‑1 (MQ=255)
                                                                                                                                                           tgtatttaagtcgggTGTGCCACGTACTGGTCTTGCTTCGGCCGAACCCAGGCCCGAGTGCCGGAAGTCTCTTGCGGGTTTTGCAGGAAAACCTGCCCGTAAGCAAACGGTGTAATGCGTCTGACAATACTTAACCCCAAACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCg                                                                                                                                    <  1:238761/276‑1 (MQ=255)
                                                                                                                                                                 tataTCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCt                                                                                                                                                            >  1:450076/1‑259 (MQ=255)
                                                                                                                                                                              gtaaaagacagtctttcttCAGCCGTACCCAGGCCCGCGTGCCGAAAGTCACTTGCCGGTTTTGCAGGAAACACTGCCCGTGCTGCAACGGGGTAATGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGtt                                                                                                                 <  1:764361/278‑1 (MQ=255)
                                                                                                                                                                                   tccTGTTCTTTCTCCAGCCCTACCCAGCCCCGCGTGCCGGAATTCTCTTGCCGGTTTTGCAGGAACAACTGCCCGTGATGCAACTGTGTACTGCGGCTGACAATACTTACCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCt                                                                                                                                                                                                       <  1:288193/196‑1 (MQ=255)
                                                                                                                                                                                   tACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCt                                                                                                             >  2:321588/1‑288 (MQ=255)
                                                                                                                                                                                        aTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAgg                                                                                                       >  1:538958/1‑289 (MQ=255)
                                                                                                                                                                                        aTCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAgg                                                                                                       >  2:477617/1‑289 (MQ=255)
                                                                                                                                                                                             tcttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCaa                                                                                                                                                                                             >  2:288193/1‑198 (MQ=255)
                                                                                                                                                                                             tcttcAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTc                                                                                                   >  2:360142/1‑288 (MQ=255)
                                                                                                                                                                                             tctcccgCCGAACCCAGCCCGGTGTGCGTGAAGGCTCTGGCCAGTTTTGCAGGAAAAACTGCCCGTAATGCAATTGGGTAAGGCGGCTAAAAATACTCAACCCCAGCCCAGCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCg                                                                                                  <  1:604842/283‑1 (MQ=255)
                                                                                                                                                                                                  ctccagcaccatgcacgtgggccgtagatctcTTACCGGTTTGCAAGGAAGATCCGACCGTGATTAAACTGTGTACGGCGCCTGACAATAATTCACCCCAGGCCAACCCCGCCATAACGGCTGTCCATAAGTACAAACGCTTTACTCAACTCCCCGCATCTACTCTCATCAATCCCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATa                                                                                             <  1:786721/261‑1 (MQ=255)
                                                                                                                                                                                                    ccGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTACTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAgg                                                                                                       >  2:791220/1‑277 (MQ=255)
                                                                                                                                                                                                    ccGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAgg                                                                                                       >  2:764361/1‑277 (MQ=255)
                                                                                                                                                                                                     cGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATa                                                                                             <  2:251846/286‑1 (MQ=255)
                                                                                                                                                                                                       tACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCt                                                                                                                                                            <  2:238434/221‑1 (MQ=255)
                                                                                                                                                                                                       tACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCt                                                                                                                                                            >  1:238434/1‑221 (MQ=255)
                                                                                                                                                                                                       tACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACAt                                                                                         >  1:329654/1‑288 (MQ=255)
                                                                                                                                                                                                         cccAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTT‑ACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCg                                                                                      >  2:89310/1‑288 (MQ=255)
                                                                                                                                                                                                                 cgcgTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAggg                                                                              >  2:580620/1‑289 (MQ=255)
                                                                                                                                                                                                                   cgTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATTACCGCCCCGTCATCTTCTTCCACCTTAATCATAATGtt                                                                                                                                               >  1:372891/1‑222 (MQ=255)
                                                                                                                                                                                                                   cgTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGtt                                                                            >  2:243622/1‑289 (MQ=255)
                                                                                                                                                                                                                      gCCGGAATTCTCTTGCGGGTTTTGCAGGAAATACGGCCCGTGATGCAACTGTGTAATGCGGCTGACATTACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACATACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTTGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                                         <  2:446890/289‑1 (MQ=255)
                                                                                                                                                                                                                      gCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                                         <  2:538958/289‑1 (MQ=255)
                                                                                                                                                                                                                      cccGTAAGTTACTGGCTGGTTTTGCAGAAAACCCTGCCCGTGATCAAACCTTGTAATCCGTCTAACAATACCT‑CCCCCAACCCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                                         <  1:89310/287‑1 (MQ=255)
                                                                                                                                                                                                                        cgtaacgtttttGCCGGTTTGGCAGGAAGAACTTCCCTTGATGCAACGGTGTAATTCGGCTGACAATACTTAACCCCATACCAAACCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATc                                                                       <  2:267610/279‑1 (MQ=255)
                                                                                                                                                                                                                                 ctTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAAc                                                              >  2:618788/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gCCCGTTTTCCAGAAAAAACTGCCCGTGATCCAACGGGTTAATTCGGCTGACAATACTCAACCCCACACAATCCCCGCCATAGCGGCTGTCCATCCGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCACTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGt                                                           <  1:744694/289‑1 (MQ=255)
                                                                                                                                                                                                                                            tGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                    >  2:219710/1‑288 (MQ=255)
                                                                                                                                                                                                                                              cAGGAAAACCTGCCCGTGTTACAACTGTGTAATGCGGCTGAAAATCCTTAACCCCAGACCAACCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggcg                                                 <  1:253280/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaCTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctct                                          <  2:511948/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGt                                                                                                                                                                                                                                                                  <  1:95047/43‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                                         >  2:558690/1‑228 (MQ=255)
                                                                                                                                                                                                                                                                                   cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCa                                                                         <  1:558690/228‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg             >  2:722456/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                   cTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGACGGGTTTGCTGTCGCGGGt            >  1:499794/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         aacattAACCCCACCCCATCCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa      <  1:580620/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aTACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa      <  1:306206/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aTACTGACCCCCAGCACAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTATCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCTTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa      <  1:449203/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               aaCCCCAGACCAATCCAGCCATAACGGCTGTCCATACGTCCAAACGCTTTACTCAACTCCCCGCATTTGCTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATCACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTCCGGGCTGTACCGACGGGCGTTTTCTACCAGGTTTCGCTATCACAAACTACGCCGGGTTGCCTCCACCTGAAAGGTGATGTCGCCGGCGCCGTCTGGCAAGAGCCGGGCTGTCCGTCGCCGGTCGAGctggct  >  1:318828/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                                                aCCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGCTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCTGCCCCTTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCgcccagggt  >  1:210653/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             
GATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGT‑GCAATGAAAAACCGTGTTTATGAAAGTTTAACTACCGTGTTCAGCGTGCTGGTGGTCAGCAGCTTTCTTTATATCTGGTTTGCCACGTACTGATCTTTCTTCAGCCGTACCCAGGCCCGCGTGCCGGAAGTCTCTTGCCGGTTTTGCAGGAAAAACTGCCCGTGATGCAACTGTGTAATGCGGCTGACAATACTTAACCCCAGACCAATCCCGCCATAACGGCTGTCCATACGTACAAACGCTTTACTCAACTCCCCGCATTTACTCTCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGT  >  NC_000913/4332023‑4332595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A