Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F9 I0 R1	113	83.2	1840812	91.6%	1686183	224.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,824,731	T→G	22.1%	V73G (GTG→GGG)	trmH →	tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,731	0	T	G	22.1%	142.7 / 2.7	70	V73G (GTG→GGG)	trmH	tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base T (11/39); new base G (16/3); total (28/42)
Fisher's exact test for biased strand distribution p-value = 3.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCC  >  NC_000913/3824507‑3824966

aTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGAAGGAAA‑AGCTGGGTACAG‑‑GGGAAAAAACACCGc                                                                                                                                                                                                                                      >  1:435017/1‑238 (MQ=255)

     ttATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGTGTAAA‑AGCTGGGGACCA‑‑GGGAAAAACCAACGCAcc                                                                                                                                                                                                                                   >  1:300706/1‑236 (MQ=255)

      tATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTCGTAAA‑AGCCGGGTACAG‑‑GGGGAAAAACACCCCACCATTg                                                                                                                                                                                                                               >  1:505385/1‑239 (MQ=255)

               aaaccGTTTTGCCCGTATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTTTGCATGGAGCAGTTCCCAAAACCTCATAACGTTTCTGCGATGATTCGTACCGCAGATGCCGTTGGCGTACACGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCCACCGCACCATTGGCGATGCCg                                                                                                                                                                                                                      <  2:897345/235‑1 (MQ=255)

                    gtttggCACGCATCTGGGAAATGCCCGCCGGGCGTCAGCCTGATCTGCCCGTCTGATTGGCGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATCCGTCCCGCAGATGCCGTTGGCGTACAGGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCt                                                                                                                                                                                                                 <  2:680441/234‑1 (MQ=255)

                      tacgcgcgagtgtgggAAATTCTGGCAAGGCGGCCGCCTGATCTTGCCGTCTGCATGGGAGAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGGCGTTGGCGTACATGAAGTTCACGCCGTCCGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAGGTGTGAAAACACACCGCACCATTGGCGATGCCGTCGCt                                                                                                                                                                                                                 <  2:622313/224‑1 (MQ=255)

                      tATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAACGTCCGGGGCACGG‑‑GGAAAAACACCCCGCACAATGGGGGAtccgggcgtca                                                                                                                                                                                                                >  1:558235/1‑229 (MQ=255)

                           gcGCCTCTGCGAAAGGCTCCCCGGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTTTTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa                                                                                                                                                                                                          <  2:435017/238‑1 (MQ=255)

                           ccGCACCGGCAAAATTCCCGCCGGGGGGAAGCCTGATGTGCCCGTTTGGATGGGGCAGGTCCACAACCCTCATAACGTTTGTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa                                                                                                                                                                                                          <  2:834777/238‑1 (MQ=255)

                              cATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGGACAG‑‑GGGAAAAACAACCGCCCCATTGGCGAGGCCGGCGCTCATCCCAAAg                                                                                                                                                                                                       >  1:523301/1‑239 (MQ=255)

                               aTCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAACTGCGGGTGGGACA‑AGCTGGGTACAG‑‑GGGAAAAAACCCCCACCCACTGGCGAAGACGTCGCTCacaccaaaag                                                                                                                                                                                                      >  2:19619/1‑230 (MQ=255)

                                 ttGCGAAATTCCCGCCAGGCGGCAGCCTTATCTGACCTTCTGGATGGAGCAGGTCCACAAACCTCATTACGTTTCTGCGATTATGCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGcc                                                                                                                                                                                                    <  2:588905/238‑1 (MQ=255)

                                 ccgagaatggCTCGCCGGGCGGCGGGTTGATTCGACCGTCGGCATGGAGCAGGGCCACAAACCGAATACCGTTTCTGCGGTTATTCGTACCGCAGATGCCGTTTGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGc                                                                                                                                                                                                     <  2:183429/229‑1 (MQ=255)

                                 cTGGGAAATGGCCGCCAGGGGGAAGCCGTATCTGACCGTTTGCATGGAGCAGGCCCACAACCCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGcc                                                                                                                                                                                                    <  2:523301/239‑1 (MQ=255)

                                 cTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTAAAG‑‑GGGTAAAAAACCCGCACCATTGGGGATGACGTCGCCCATCTCCAAAGcc                                                                                                                                                                                                    >  1:177266/1‑239 (MQ=255)

                                         tGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGGTGCCGTTCGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGGGTACA‑AGCTCGGTCCCA‑‑GCGAAAAACAACCGCCCCCTTGGGGCTGCCGCGCCCCATCTCaaaaagcaaggcgtg                                                                                                                                                                                            >  2:340221/1‑227 (MQ=255)

                                              gCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GGGGAAACACACCGCACCATTGGCGCTTCCGTCGCTCATCTCAAAGGCCCGGGCaatcaaaa                                                                                                                                                                                       >  1:817889/1‑232 (MQ=255)

                                                          ccTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGAACCATGGCTTCGGCAGCGGCGGGTCGTAAC‑AGCGGGGTTCAG‑‑GGGGAAAAACACCCCCCCCATTGTGATGCCCTCGCCCCCCTCAACAGCCAGGGGCCTCCG‑ATTC‑TGTCAAcccc                                                                                                                                                                         >  2:529694/1‑238 (MQ=255)

                                                              aTCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCtt                                                                                                                                                                     <  1:909815/239‑1 (MQ=255)

                                                                    ccttctgctggggagaGGTCCACAACCTCCATAACTTTTCTGGGATTATTGGTCCCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGa                                                                                                                                                                <  2:89323/223‑1 (MQ=255)

                                                                        cttcctGGGGCCGGTCCCCAAACCCTATAACGTTTCGGGGATTTTTCGTCCCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACg                                                                                                                                                           <  2:817889/234‑1 (MQ=255)

                                                                        ctcccttggtctggTTCACAAGCCTTATAACGTTTCTGCGATTATTCGGACCGCGGATGCCTTTGGCGTCCATGAAGTTCAGGCCGTCTGGGCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGGACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACg                                                                                                                                                           <  2:847786/227‑1 (MQ=255)

                                                                        cTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGGGTAAC‑ACCGGGGGACCG‑‑GGGAAAACACACCGCCCCCCAGGCGAGGGCGTGTCTCCACTCAAAGGCCAGGGACCGCAGAATTC‑T‑TCAACCCCCCTTTCTggttaa                                                                                                                                                            >  2:154430/1‑233 (MQ=255)

                                                                        cTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGGTCAG‑‑GTGAAAAAACACCGCACCCTTGGCGATGCCGTGCCTCCACTCAAAAGCCAGGGCAAGCAG‑ATTC‑TGGCAACACACATTTCTGTTaaa                                                                                                                                                            >  1:14805/1‑237 (MQ=255)

                                                                                  cAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCAACCCCACCATTGGCGAGTCCGCGGCTCATCTCAAAGGCCAGGGCATGCCA‑ATTC‑TGGCAACCCATCTTTATGGTAACGCTG‑T‑CGAtt                                                                                                                                                >  1:42770/1‑238 (MQ=255)

                                                                                          caacccctaaaaCGTTTCGCCGATTATTCGTACCGCGGTAGCCGGTTGGGTACAGGAAGTTCACGCGGTCGGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGaaa                                                                                                                                       <  2:794056/229‑1 (MQ=255)

                                                                                          caAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCGGGGAACAG‑‑GGGAAAACACCCCCCACCCTAGGCGTTGCCGCCGCGCATTTCAAAGGCCCGGGCGTACAG‑AATT‑AGGGACACCATCTTTTTGCTAACGCCG‑T‑CGCTGTcccgcaaa                                                                                                                                       >  2:884071/1‑233 (MQ=255)

                                                                                               cTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGGCAAG‑‑GGGAAAACACACCCCACCATTGGCGATGCCGTCGCTCATCTCCAAAGCCAGGGCATGCAG‑ATTC‑TCGCAAACCACCCTTCTTATAACCCCG‑T‑GGAGTTTCGCGGAAATGAt                                                                                                                                  >  2:481419/1‑239 (MQ=255)

                                                                                                   tAACTTTTTTGCCATTTTTCGGTCCGCGGATGCCGTTGGCGTACATGAAGTTCCCGCCGTCTGGCCCGGGAGCCGGATGCGGACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTaca                                                                                                                              <  2:119051/239‑1 (MQ=255)

                                                                                                          tccgCGATTACTCGTACCGCATATGCCGTTGGGGTACATGAAGTTCACGCCGGCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGccc                                                                                                                       <  1:612198/236‑1 (MQ=255)

                                                                                                            tGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑CGCTGGGTACAG‑‑GGGAAAACACACCGCACCACTAGCGATGCCGCCGCTCATCTCCAAAGCCAGGGCGCGCCG‑CTTC‑TGGCCACCCACCTTTCTGTTTACACTGTT‑CGATTACCGCAAAAtatataaaccccccccc                                                                                                                      >  2:384830/1‑223 (MQ=255)

                                                                                                                 ttattCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCCGGGTGCAG‑‑GGGAAAACACACCCCCCCCCTGGGGATGCCCTCGCTCCACTCAAAGGCCAGCGCCTGCAA‑ATTCGTGACACCCCA‑CTTTTTCATAACGCTT‑T‑CCGATTCTCCCAAATTGTTTaccaccccccccccac                                                                                                                 >  2:487326/1‑224 (MQ=255)

                                                                                                                   attCGTAACGCAGATGCCTTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAt                                                                                                              <  1:529694/239‑1 (MQ=255)

                                                                                                                           cGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGGGATGCCGTCGCTCATCTCACAGGCCAGGGGCTGCGA‑ATTC‑TGGCAACCCATCTTTCTG‑TAAAACCG‑TGCGATTTCCCCGCAATTTATTACACACcccccaaccgacttttggggg                                                                                                      >  2:328856/1‑222 (MQ=255)

                                                                                                                                        ggCGTACATGAAGTTCCCGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAc                                                                                         <  1:481419/239‑1 (MQ=255)

                                                                                                                                        ggCGTACATGAAGTTCCCGCCGTCTGGCCTGGTAGCCGCATGCGCACCAGGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAc                                                                                         <  1:791276/239‑1 (MQ=255)

                                                                                                                                          cGTACATGAAGGTCACGGCGGCTGGCCTGGTAGCCGCATGCGGACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACCCCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACg                                                                                        <  1:411666/238‑1 (MQ=255)

                                                                                                                                                tGAAGTTCCCGGCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑Ca                                                                                <  1:19619/239‑1 (MQ=255)

                                                                                                                                                   atttCCCGCCGTCTGGCCGGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGc                                                                             <  1:566624/237‑1 (MQ=255)

                                                                                                                                                    gtccACGCCGGCTGGCCTGTTAGCCGCATGCGAACCAGGGCTTCGGCGGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGa                                                                                                         <  2:851264/208‑1 (MQ=255)

                                                                                                                                                      tccccccctttGGCCGGGTAGCCGGAGGCGGACCAGGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAgg                                                                          <  2:847275/229‑1 (MQ=255)

                                                                                                                                                         cGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCGGGGTACAG‑‑GGGGAAACACACCCCCCCATTGGCGAATCCGTCGCTCCTCTTAAAAGCCAGGGCCAGCAG‑ATTT‑TGGCAACCCCTCTTTTTGTTAACCCTG‑A‑CGATTTCCGCGAAATTGATTTTACTCTCCCCACCCGCACTTTTTTGGGGCGGTGGAAAAAggggaaaaagccgagga                                                                        >  2:692898/1‑225 (MQ=255)

                                                                                                                                                               cTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GGGAAAACACACCCCACCCTTGGCGATGCCGTCGCTCATCTCAAAAGCCCGGGCATCCCG‑ATTC‑TGGCAACCCCCCTTTTTCTTAACGCTG‑G‑TGCGTTCCCCGCAACTGGTTACACTCCCCCGACCTTCATTTTTCTGGGGCACGAGAAAAACGGCAT‑ACCGCCGGGAGCA‑ATg                                                                 >  2:195580/1‑238 (MQ=255)

                                                                                                                                                                  gCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGaa                                                                                                                                        <  1:422672/166‑1 (MQ=255)

                                                                                                                                                                     tgtgcgccccagcgcgaCCCTGGCTTCGGCAGCGGCGGGTAGTAAG‑TGCGGGGTACCG‑‑GTTAAAACCCACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg                                                          <  2:888807/223‑1 (MQ=255)

                                                                                                                                                                     tGGTAGCCGCATGCGCCCCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg                                                          <  1:556948/239‑1 (MQ=255)

                                                                                                                                                                     tGGTAGCCGCATGCGACCAATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGGTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg                                                          <  1:884071/239‑1 (MQ=255)

                                                                                                                                                                     ggttggCCGCGTGCGCACCATGGCTTTGGGCGCGGGGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACCCCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCAGCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTgg                                                          <  2:602548/234‑1 (MQ=255)

                                                                                                                                                                           cgcgatacgcgCCTTGGCTTCGGCAGGGGCGGGTAGTAAC‑CGCGGGGTACAG‑‑GGGAAAACACGCCGCCACATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGAt                                                     <  2:177266/231‑1 (MQ=255)

                                                                                                                                                                           ccccgggtgcgcaaTGGCTTCGGGAGGGGCGGGTAGTAGC‑AGCTGGGTACAG‑‑GGGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTTCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATc                                                    <  2:558235/226‑1 (MQ=255)

                                                                                                                                                                           ccGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAAC‑AGCCCGGTTCAC‑‑GTGAAAACACACCGCACCCTTTGCGGTGCCCTCCCTCATCCCAAAGGCCAAGGCCTACCG‑ATTC‑TTGCTACCCATCTTTCTCATAACGCTG‑T‑CGATTTACCCGAAATTTACTACACCCGCCCCACCTGCATCTTTCTGGGACACTGGAAAACAGCGATCCACCCAGAAACAA‑TTGGCCCTgcgggag                                                     >  2:412554/1‑233 (MQ=255)

                                                                                                                                                                            cGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATc                                                    <  1:381917/238‑1 (MQ=255)

                                                                                                                                                                                  gccccccaggCTCCGGCAGGGGCGGGTAGTAAC‑AGCCGGGGACAG‑‑GTTAAAACCCACCGCACCATTGGCGATGCCGTCGCCCAACTAAAAGGCCAGGGCATGCAG‑ATTC‑TGGCATCCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGa                                                <  1:340221/228‑1 (MQ=255)

                                                                                                                                                                                   gccccATGGCTTCGGCAGGGGCGGGTTGTAAC‑AGGTGGGTACAG‑‑GGGAAAACACACCGCCCCATTGGCGATGCCGGCCCTCATCCCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCATGGCGGATCAGGAcat                                             <  2:902502/235‑1 (MQ=255)

                                                                                                                                                                                     aCCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACCCACCGCCCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGAcatcat                                          <  1:66221/239‑1 (MQ=255)

                                                                                                                                                                                      ccATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGGTAAAGCTG‑T‑CGATTTCCGCGAAAATTATTAAACTCGCCCGACCTGCATTTTTGTGGGAACGGGGAAAACAGGCCT‑AACGCCCGAAGCACTTTGCCCTgcgggacagagaaacaaa                                          >  2:585696/1‑222 (MQ=255)

                                                                                                                                                                                          ggCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa                                                                                                                                                                                                          <  2:660758/80‑1 (MQ=255)

                                                                                                                                                                                          ggCTTCGGCAGCGGCGGGTAGGAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCa                                                                                                                                                                                                          >  1:660758/1‑80 (MQ=255)

                                                                                                                                                                                            cTTCGGCAGGGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg                                   <  1:277497/239‑1 (MQ=255)

                                                                                                                                                                                             ttCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTTCAG‑‑GTAAAAACCCACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg                                   <  1:692898/238‑1 (MQ=255)

                                                                                                                                                                                             ttCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCg                                   <  1:293721/238‑1 (MQ=255)

                                                                                                                                                                                                 gccggggcggGTGGTAAC‑CGCTTGGTACCG‑‑GTGAAAACACACCGCCCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCgatgat                              <  2:300706/234‑1 (MQ=255)

                                                                                                                                                                                                              tgtacacgCTGGGTCCAG‑‑GTGAAAACCACCCGGCCCTTTGGCGATGCCGTCGCTCAGCGCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTGCGGATAACGCTG‑T‑CGAGTGCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGAGGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa                  <  2:405285/232‑1 (MQ=255)

                                                                                                                                                                                                              gtaAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa                  <  1:384830/238‑1 (MQ=255)

                                                                                                                                                                                                              gtaAC‑AGCTGGGGACAG‑‑GTGAAAACACACCGCTCCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCa                  <  1:74814/238‑1 (MQ=255)

                                                                                                                                                                                                                      cTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGCCAGGAGAAAACGGGCAT‑AACGCAGGAAGCA‑TTGGCCCTGCCGGATCAGGACATCCTCTTTCCGATGACCGGCCTGGTGCGGTccatga          >  1:548275/1‑234 (MQ=255)

                                                                                                                                                                                                                      cTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CCCCCCGGAAGCA‑TTGGCCCTGGCGGGTCAGGAAAACCGCATTCCGATTATCGGCCTGGGGCCAGCGCTGa          >  2:74138/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGCCAGTAGAAAACGGGCAT‑AACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATg       >  1:851858/1‑239 (MQ=255)

                                                                                                                                                                                                                         ggTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAAAGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAAAGGGCAT‑CACGCAGAAACAC‑TTGGCCCCGGCGGATCAGAACATAACAATTCCGATAACCGGCATgggccatgcggtga          >  2:728082/1‑224 (MQ=255)

                                                                                                                                                                                                                              aG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGGTTcc  >  1:561443/1‑239 (MQ=255)

ATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAAC‑AGCTGGGTACAG‑‑GTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAG‑ATTC‑TGGCAACCCATCTTTCTGATAACGCTG‑T‑CGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAT‑CACGCAGGAAGCA‑TTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCC  >  NC_000913/3824507‑3824966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A