Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,826,737 A→G 11.1% E510G (GAG→GGG)  recG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,826,7370AG11.1% 271.4 / 2.7 116E510G (GAG→GGG) recGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (51/48);  new base G (8/9);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGC  >  NC_000913/3826507‑3826974
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
cACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCCGGGAAGGGTTGAAAc                                                                                                                                                                                                                                        >  1:555926/1‑239 (MQ=255)
     tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGgc                                                                                                                                                                                                                                    >  1:383435/1‑238 (MQ=255)
     tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGgc                                                                                                                                                                                                                                    >  1:1066309/1‑238 (MQ=255)
               tGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAGGCTACCTGGGAAAAGGTGAAAATgggggaaccggagg                                                                                                                                                                                                                         >  2:299156/1‑227 (MQ=255)
                 cGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGTTTGAAGGGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGTTACCTGGGAAGGGGGGGAACGGGCGCtccagtggtgg                                                                                                                                                                                                                       >  2:749688/1‑229 (MQ=255)
                 cGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGGGCAGGCGGGGGAGGCTACTTGGGAAAGGTTGAAACTGGGGGTACCAGGGTTg                                                                                                                                                                                                                       >  2:87759/1‑239 (MQ=255)
                 cGTATGCCGATCTCGATACCTCGGAGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGGGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAACCTGACGCTACCAGAGCTg                                                                                                                                                                                                                       >  1:680950/1‑239 (MQ=255)
                   tATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGaa                                                                                                                                                                                                                     >  1:680213/1‑239 (MQ=255)
                      gCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGACGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGCAGAGTTGAAAATGgcctcacccaggtttgaagt                                                                                                                                                                                                                  >  2:33/1‑221 (MQ=255)
                           tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGCGTCGGAATTGCTGGAAGCGCAGGCGGCGGAGGCTACCTGGGAAGAGGTTAAACTGGCGCTACCAGCGtttgaaattgg                                                                                                                                                                                                               >  2:46898/1‑228 (MQ=255)
                           tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCGACCTGGTAAGAGTTGAAACTGGGGCTACCAGAGTTGAACATTGGcc                                                                                                                                                                                                             >  1:669024/1‑239 (MQ=255)
                                aTACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGCCGGAATTGCTGGCAGCGCAGGCGGCGGACGCTACCTGTGAAGGGTTGAAACTGGCGCTAcccaggtggagaagtgcgcgggaa                                                                                                                                                                                                        >  2:902016/1‑217 (MQ=255)
                                      cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGATTCGGAATTGCTGGAAGCGCAGGCGGCGGCAGCTACCTGGGAAGGGTTGAAACTGGCCCACCCAAGGTTGAACCTTGGCCTggacaacggg                                                                                                                                                                                                  >  2:1050305/1‑231 (MQ=255)
                                      cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGGATTGCTGGAAGCGCCGGCGGCGGAAGCTTCCTGGGAAGGGTTGAAACTGTCCCTACCGGAGTTGAACTTTGGCCTGGGACACggg                                                                                                                                                                                                  >  2:796804/1‑239 (MQ=255)
                                       gtgaatgaggggccccccgcccgcccgcGCGCGAGTCACTCCGGTCGTTATTCCGGATCCCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACggg                                                                                                                                                                                                  <  1:900353/222‑1 (MQ=255)
                                               aTGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGGGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGGCGTACCAGGATTGAACGTGGGCCTgggcaccgggggggggaa                                                                                                                                                                                          >  2:1062107/1‑222 (MQ=255)
                                                                         ggtatcggctgtgtctctctcgATTCCCGCCGTACCGAAATCATTGACCGCGTGCCCCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGc                                                                                                                                                               <  1:143899/218‑1 (MQ=255)
                                                                          tacccaCGGTCGTTTTTCCTGATCCCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGc                                                                                                                                                               <  1:87759/233‑1 (MQ=255)
                                                                           ggatgcggtcggtATCGCTGACCGCCGCCGTACCGACATCATTGACCGCGTGCACCGCGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGcg                                                                                                                                                              <  1:653550/233‑1 (MQ=255)
                                                                                 ggTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGCAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGGTGGCGTCGtttt                                                                                                                                                       >  1:216754/1‑238 (MQ=255)
                                                                                 ggTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGACACTGCCGCTACCAGAGTTGAACGTTGGCCTGTTACACGGGCGGATGAAAc                                                                                                                                                                                        >  1:1056839/1‑206 (MQ=255)
                                                                                 gctcctttttccagcccccccccGTACCGACATCATTGCCCGTGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTa                                                                                                                                                       <  2:54932/222‑1 (MQ=255)
                                                                                     gCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGGGAAACAGGCGGGGATGGCGCCGTTTaaaac                                                                                                                                                   >  1:239428/1‑237 (MQ=255)
                                                                                       tctcccttacccccGCCGTCCCGACATCATTGACGGGGTGCACCGCGCCTGACTAACTGAAGGTCGGCAGGCATACTGGGGTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAg                                                                                                                                                 <  2:96809/228‑1 (MQ=255)
                                                                                       cggcccggtgccccGGCGGACCGAAATCATTGACCGGGTGCACCACGGTGGCATAACTGAAGGTCGTCAGGAATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAg                                                                                                                                                 <  1:1028741/228‑1 (MQ=255)
                                                                                              ccccccccccGTACCGACATCTTGGCCCGCGTGACCCACGCCGGCATACCTGAAGGTCGTCAGGCGTGCGGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCt                                                                                                                                         <  2:940724/235‑1 (MQ=255)
                                                                                               aTACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGGTACCCAAGTTAAACGTTGGACGGGTACACGGGCGGATGACACCTGCCGGGAAACAGGCGGGGTAGGGGTGGTTTAAAAAAgaggggata                                                                                                                                         >  2:355496/1‑231 (MQ=255)
                                                                                                   ccgccgTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGCAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGGGAAACAGGCGGGGCTGGCGTCGGTTTAACAAGGTGG‑GCttccc                                                                                                                                     >  1:847200/1‑234 (MQ=255)
                                                                                                   ccgccgTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGCCGGTGATGGCGTCGTTTAAACAAAGGGA‑GCTacac                                                                                                                                     >  1:743145/1‑238 (MQ=255)
                                                                                                     gccgTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAAAAGGCGGGGGGTGCGTGGGTTAGACAAAGGGA‑GCTacaaccg                                                                                                                                  >  2:1028741/1‑235 (MQ=255)
                                                                                                        gtccccaCATCATTTACCGCGGGCCCCCCGCTTGGAATAATGAAGGTCTGACGGTATGTGGGGTTTGTGCGGTGATGGAAGAGTCGGAATGGCTGGAAGCGCAGGCGGCGGAAGCTACCGGGGAAGAGTTGAAACTGGCGCTACCGGAGTTGACCGTTGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACctgctg                                                                                                                               <  2:441752/233‑1 (MQ=255)
                                                                                                         tACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGGGGTGATGGGGTCGTTTTAACAAAGGGT‑GCTACACCTGCggg                                                                                                                              >  1:85084/1‑239 (MQ=255)
                                                                                                          cccGACAGCATTGACCTCGTGCATCATGCTTGGATAGCGGAGAGTCGTCAGGCTGATGGGGTTTGGACGTTGATTGAAGCGGCGGTATGGCTGGAAGTGCAGGGGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTGGAACGTTGGCTGGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTTATGGCGTCGTTTAAACAAGGTGA‑GCTAAACCTGCTGGt                                                                                                                             <  2:680950/238‑1 (MQ=255)
                                                                                                             gACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCGGCCGGGAAACAGGCGGGGAGGGGGGCGGTTAAACAAAGTgggagcaaactgcggggtgc                                                                                                                           >  1:1026365/1‑220 (MQ=255)
                                                                                                              aCATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTCCAACTCCTGGTTGcc                                                                                                                         >  1:98072/1‑239 (MQ=255)
                                                                                                               catcatTGGCCGCGTGCCCCCCGCCTGCATAACTGGAGGTCGGCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACctg                                                                                                                                  <  2:73496/229‑1 (MQ=255)
                                                                                                                tatatTGGCGGCTTGCACCGCGGCTGCATAACTGGAGGGCGTCGGGCATGCTGGGTTTGTAAGTTGATTGAAGAGTCGGAATTGCTGGGAGCGAGGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCcac                                                                                                                       <  2:902308/236‑1 (MQ=255)
                                                                                                                atcatTGACCGGGTGCACCGCGGCGGCATAAGTGAAGGTCGTCAGGCATACTGGGTTTGTACGTGGATTGAAGAGGCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACGGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCcac                                                                                                                       <  1:503142/239‑1 (MQ=255)
                                                                                                                atcatTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGCAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGGACACGGGGGGATGAAACCTGCCGAGAAACAGGGGGGGATAGGTGCTTTTAAAAAAGGGGA‑GCtcaacaccccggtggcgcc                                                                                                                       >  2:795308/1‑221 (MQ=255)
                                                                                                                 tcagTGACCGCGTGCACCACGGCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCcac                                                                                                                       <  1:366418/238‑1 (MQ=255)
                                                                                                                   atTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTCCCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGTTGGCGGCCTTTCAACACGGGGG‑GCTACCCctcccggggtccccaac                                                                                                                    >  2:1086711/1‑224 (MQ=255)
                                                                                                                     tGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCCACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGGGCGGGGGTGGTTTAAAAAAGGTGG‑GGCACACCGCCCGGGTGCGACAACCg                                                                                                                  >  2:733614/1‑239 (MQ=255)
                                                                                                                         cgggTGCACCACGGCTGCATAACTGAAGGTCGGTAGGCATCCTGGGTTTGGACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTa                                                                                                               <  1:423308/235‑1 (MQ=255)
                                                                                                                         cgcgTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCCTCGTTTAAACCAGGTGA‑GCTACACCTCCTGGTTGCcacccgtccctt                                                                                                              >  1:73496/1‑230 (MQ=255)
                                                                                                                             tGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAACCAGGCGGGGATGGGGGCGTTTTAACAAGGGGG‑GCTAACCCTGCTGGTTGCCACAAACggtttttaa                                                                                                          >  1:575345/1‑231 (MQ=255)
                                                                                                                               ccccccGCCTGCATAACCGAAGGGCGCCAGGCATACTGGGTTTGGACGTTGATTGAAGAGGCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGGTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGt                                                                                                        <  2:847200/234‑1 (MQ=255)
                                                                                                                                ccccgcccTGCAAAAATGAAGGGTGTCAGGGATTGTGGGTTTGGCCGTTGATTGAAGGGTCGGAATTGCTGGAAGGGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGt                                                                                                        <  2:897181/232‑1 (MQ=255)
                                                                                                                                cccacGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑g                                                                                                                                           <  1:543545/202‑1 (MQ=255)
                                                                                                                                accacGCCTGCATAACTGAAGGTCGGCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAAc                                                                                                                    <  1:375098/226‑1 (MQ=255)
                                                                                                                                      ccTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGGGG‑GCCTAAACTGCGGGTTGCCACAACCGGTTTTGAAGTCgggggt                                                                                                 >  1:897181/1‑235 (MQ=255)
                                                                                                                                        tGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGGTGGGGTCGTTTCAAAAAGGGTG‑GCTACACCCGCCGGGTTCCACCACCCGTATTgtaatccggggttt                                                                                               >  2:835137/1‑226 (MQ=255)
                                                                                                                                        tGCATAACGGAATGTCGTCAGGCATACGTGGTTGTTACGTTGATTGAAGAGTCGGATTTGCTGGAGGCGCGGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGa                                                                                               <  2:367382/239‑1 (MQ=255)
                                                                                                                                         gCATAACTGAAGGTCGTCAGGGATACTGGGTTTGTACGTTGATTGAAGAGGCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGAt                                                                                              <  1:796804/239‑1 (MQ=255)
                                                                                                                                         gCATAACTGAAGGTCGGCAGGCATACTGGGTTGGTACGTTGATGGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGAt                                                                                              <  1:835137/239‑1 (MQ=255)
                                                                                                                                          cATAACTGAAGGTCGTCAGGCATATGGGGTTTGTACGTTGATGGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTAAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGAt                                                                                              <  1:355496/238‑1 (MQ=255)
                                                                                                                                          cATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGGGGCGATGGCGTCGGTTAAACAAAGTGG‑GCTACACCCTCTGGTTGCCACAACCGTTTTTTGAGTCGGGGTTGAtg                                                                                             >  1:62320/1‑239 (MQ=255)
                                                                                                                                          aaTACCTGAAGGGCGTCGGGCTTCTTGGGTTTGTCCGTGGATGGAAGAGTCGGAATTGCGGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGAtg                                                                                             <  2:743145/238‑1 (MQ=255)
                                                                                                                                           cagaCTGAAGGGCGTGAGGCATACTGGGTTTGTACGTTGATTGAAGAGGCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGAtg                                                                                             <  1:336715/235‑1 (MQ=255)
                                                                                                                                               cTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTTAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTTAAGGCGGCGGTGATGTGGCt                                                                                        >  1:929052/1‑239 (MQ=255)
                                                                                                                                                 gAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGCTGAAACCTGCCGAGAAACAGGCGGTGATGGCGGCGGTTAAACAAGGCGA‑GCTAAACCTGCTGGTTGCCCCAAACGTTATTTAAGCCGGCGTTggttggccca                                                                                       >  1:330739/1‑229 (MQ=255)
                                                                                                                                                    ggTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAAAAAGGTGA‑GCTACACCCGCTGGGTGCCACAACCGGTATTTAAATCGGCGTTGGTGTGCCTAACg                                                                                    >  1:876662/1‑238 (MQ=255)
                                                                                                                                                     gtcgtcAGGCTTATTGGGTTTGTTGGTTGATTTAAGGGTAGGAATTTGTGGAAGCGCAGGCGGGGGAAGCTACCGGGGAAAGGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACg                                                                                    <  2:1045507/237‑1 (MQ=255)
                                                                                                                                                      ttttcAGGCATTTGGGGTTGTTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTGAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGc                                                                                   <  2:680213/234‑1 (MQ=255)
                                                                                                                                                      tcgtcAGGCATACTGGGTTTGTAAGTTGATTGAAGAGTCGGAATGGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACTCCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGc                                                                                   <  1:144552/237‑1 (MQ=255)
                                                                                                                                                       cgtcAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGCACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGGAACAGGCGGGGATGGCGTCCTTTTAAAAAGGGGA‑GTTAAACACGCCGGCTTCCACAACCCGTTTTCTAGTCGGCGTTTGTTTGCCCAACCCCAg                                                                                >  2:707855/1‑239 (MQ=255)
                                                                                                                                                       cgtcAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGGTGGCGTCGTTTTAACAAGGGGA‑GCTACACCTGCTGGTTTCCACAACCGTTTTTGAAGTCGGCGTTGATGTGTCCAACGcccg                                                                                >  1:740865/1‑237 (MQ=255)
                                                                                                                                                         tcAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCt                                                                                                                                         >  1:565754/1‑180 (MQ=255)
                                                                                                                                                         tcAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCt                                                                                                                                         <  2:565754/180‑1 (MQ=255)
                                                                                                                                                                     ggTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGACCTGGTACACGGGCGGATGAAAc                                                                                                                                                                                        <  1:711949/122‑1 (MQ=255)
                                                                                                                                                                     ggTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGACCTGGTACACGGGCGGATGAAAc                                                                                                                                                                                        >  2:711949/1‑122 (MQ=255)
                                                                                                                                                                             cGTTGATTGAAGAGTCGGAATTGGTGGAAGCGAAGGCGGCGGAAGCTTCCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCg                                                          <  1:1062107/239‑1 (MQ=255)
                                                                                                                                                                              ttttgttgaAGGTTCGGAATTGCTGGAAGCGGGGGGGGCGGAAGGTACCGGGGAAGAGTTGAAACTGGCGCTACCAGGATTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCgg                                                         <  2:555926/234‑1 (MQ=255)
                                                                                                                                                                               ttggttgaAGGGTCGGAATTGCTGGAAGCGGAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTGGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCgg                                                         <  1:749688/238‑1 (MQ=255)
                                                                                                                                                                               ttgattgaAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGGCGTTTAACCAAGGTGG‑GGTCCACCCCCTGGGTGCCACAACCGTTTTTTAAGACGGGGTGGATGGGGCTAACGCCAGTCGCATAGTTAACGAAAAACCggg                                                        >  2:98370/1‑238 (MQ=255)
                                                                                                                                                                                     gaAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGGGA‑GCTACACCTGCTGGTTGCCCCAACCGGTATTTAAGTCGGCGTTGATGTGCCTAACGCCAGACTGCTGATTTTCCAAAACCCGGGGCg                                                     >  1:86277/1‑236 (MQ=255)
                                                                                                                                                                                          gttggAATTGCTGGAAGGGCAGGGGGCGGAGGTTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAAACGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCgtctgggt                                              <  1:257495/235‑1 (MQ=255)
                                                                                                                                                                                          gTCGGAATTGTGGGAAGCCGCGGGGGGGGAAGGTACCGGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGGACACGGGCGGATGAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCgtctgggt                                              <  2:740865/238‑1 (MQ=255)
                                                                                                                                                                                           tCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTTAACAAAGGGA‑GCTACACCACCCGGGTGCCACAACCGGTATTTAAGTCGGCGGTGATGTGGCTAACCCCAGGCTGGTGGTTTTCTAAAAACCgggagggctgggggc                                            >  2:11578/1‑226 (MQ=255)
                                                                                                                                                                                              gatttGCTGGAAGCGGGGGCGGCGGAAGCTCCCTGGGAAGAGTTGAAACGGGCTCTCCCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCCGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCgtctgggtctgg                                          <  2:876662/235‑1 (MQ=255)
                                                                                                                                                                                               aaTTGTTGGAGGGGAGGGCGGGGGAAGCTCCCTGGGAAGGGTTAAAACTGGGGCGACCAGGGTTGAACGTTGGCCTGGTACACGGGCGGATGAACCCTGCCGAGAAGCAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGgc                                         <  2:85084/238‑1 (MQ=255)
                                                                                                                                                                                               aaTTGGTGGAAGCGGGGGCGGGGGAAGCTCCCTGGAAAGGGTTGAAACTGGCGCTACCAGAGTTGAACTTTGGCCGGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGgc                                         <  2:575345/238‑1 (MQ=255)
                                                                                                                                                                                               aaTTGGGGGGAGCGGAGGGGGCGGAAGCCTCCGGGGAAGGGGTGAAACTGGCGCTACCAGGGTTGAACGTTGGCCTGGTACAGGGGCGGATGAAACCTGCCGGGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGgc                                         <  2:912293/238‑1 (MQ=255)
                                                                                                                                                                                                atgtcTGGAAGCGCAGGGGGCGGAAGCGACCGGGGAAGGGTTGAAACTGGCGCTACCAGAGGTGAACGTTGGCCGGGGACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCTTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGgcg                                        <  2:210311/234‑1 (MQ=255)
                                                                                                                                                                                                 ttGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCa                                      <  1:795308/239‑1 (MQ=255)
                                                                                                                                                                                                 ttGCGGGAAGCGCAGGCGGGGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCa                                      <  1:98370/239‑1 (MQ=255)
                                                                                                                                                                                                   gCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGGGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGGTGATGTGCCTAACGCCCGTCTGGTGATTATCGAAAACCCGGAGGGGCTGGGGCTGGCGCAGt                                    >  1:138475/1‑239 (MQ=255)
                                                                                                                                                                                                         aGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAACCAGGCGGGGATGGCGTCGTTTAAACCAGGGGAGGCCAC‑CCCGCTGGGTGCCACCACCGTTATTTGAGTCGGCGTTGTTGTGCCTAACACCAGCTTTATGGTTTTATAAAACCCGGGGCGTCTGGGGCTTGCGCGGTTAAAcc                              >  2:944514/1‑239 (MQ=255)
                                                                                                                                                                                                                  gggggAAGCTACCTGGGAAGGTGTGAAACGGGCGCTACCAGAGTTGAACGTTGGCCTGGTACCCGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGcgcg                     <  2:664846/236‑1 (MQ=255)
                                                                                                                                                                                                                  ggcggAACCTACCTGGGAAAAGTTGAAACTGGCGCTACCAGATTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTgcgc                      <  1:1086711/238‑1 (MQ=255)
                                                                                                                                                                                                                   ggggaaggttacggggAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGcgcg                     <  1:974267/225‑1 (MQ=255)
                                                                                                                                                                                                                   ggggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGcgcg                     <  1:439509/236‑1 (MQ=255)
                                                                                                                                                                                                                    cggAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACg                                                                                    <  1:776614/174‑1 (MQ=255)
                                                                                                                                                                                                                          cTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGGGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATTTGCCTAACGCCAGGCTGATGATTATCGAAAACCCGGAGGGTCTGGGTCTGGCGCAGTTAAACCAGCTTCGCGGGcgcgga             >  1:664846/1‑237 (MQ=255)
                                                                                                                                                                                                                           tcccTGGGAAGAGTTGAAACGGGCTCTCCCAGGGTTGAACGTTGGCCTGGTACAGGGGCGGATGAAACCTGCCGAGAAACGGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGt              <  2:98072/235‑1 (MQ=255)
                                                                                                                                                                                                                             ccTGGGAAGGTTTGAAACGGGCGCTCCCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCGGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAgg           <  1:412997/238‑1 (MQ=255)
                                                                                                                                                                                                                               tGGGAAGGGTTAAACTTGGCGCTACCATAGTAGAACGTGGGCCGGGGACACGGGCGGATAAACCCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCg        <  2:741787/239‑1 (MQ=255)
                                                                                                                                                                                                                                  gAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTaca                                >  1:312373/1‑212 (MQ=255)
                                                                                                                                                                                                                                  gAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTaca                                <  2:312373/212‑1 (MQ=255)
                                                                                                                                                                                                                                     gagTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTTATGATTTTCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGGTACACCAGCTGCGCGGGGGCGTCAGTCGTGgcgc  >  1:420969/1‑239 (MQ=255)
                                                                                                                                                                                                                                     gagTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGGGA‑GCTACAACTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTGTATTTGCCTTAAGCCAGTCTGATGGTTATCCGAAAACCGGGGCGGCCGGGGCTGGGCCAGGTAACACAACTGCGCGGGCGCGGAGGGCGGGgcgc  >  2:163703/1‑239 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
CACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAACAAGGTGA‑GCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCTAACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCGTGGCGC  >  NC_000913/3826507‑3826974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A