breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 12,435 | C→A | 14.1% | F91L (TTC→TTA) | dnaK → | chaperone Hsp70, with co‑chaperone DnaJ |
RA | 37,255 | A→C | 17.4% | G190G (GGT→GGG) | caiC ← | putative crotonobetaine/carnitine‑CoA ligase |
RA | 62,169 | T→C | 13.0% | S366G (AGC→GGC) | rapA ← | RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase |
RA | 71,877 | A→G | 28.7% | E176G (GAG→GGG) | yabI → | DedA family inner membrane protein |
RA | 144,085 | A→C | 19.4% | S128S (TCA→TCC) | yadH → | putative ABC transporter permease |
RA | 151,757 | A→G | 8.7% | S159P (TCC→CCC) | yadL ← | putative fimbrial‑like adhesin protein |
RA | 203,550 | T→G | 12.6% | V68G (GTG→GGG) | lpxB → | tetraacyldisaccharide‑1‑P synthase |
RA | 207,473 | T→G | 27.3% | M783R (ATG→AGG) | dnaE → | DNA polymerase III alpha subunit |
RA | 216,077 | G→C | 5.6% | intergenic (+98/+102) | nlpE → / ← yaeF | lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein |
RA | 223,706 | T→A | 7.5% | intergenic (+298/‑65) | gmhB → / → rrsH | D,D‑heptose 1,7‑bisphosphate phosphatase/16S ribosomal RNA of rrnH operon |
RA | 249,538 | T→G | 28.1% | pseudogene (533/1713 nt) | lfhA ← | pseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
RA | 289,839 | T→C | 15.2% | E156G (GAG→GGG) | argF ← | ornithine carbamoyltransferase 2, chain F, CP4‑6 prophage |
RA | 324,226 | A→G | 11.0% | S157G (AGC→GGC) | ykgG → | LutC family protein, putative electron transport chain YkgEFG component |
RA | 342,454 | A→G | 13.8% | R444G (AGA→GGA) | yahJ → | putative metallo‑dependent hydrolase domain deaminase |
RA | 357,381 | T→G | 11.3% | V404G (GTG→GGG) | codA → | cytosine/isoguanine deaminase |
RA | 361,668 | C→T | 17.5% | E65K (GAA→AAA) | lacA ← | thiogalactoside acetyltransferase |
RA | 361,670 | A→T | 18.9% | V64E (GTA→GAA) | lacA ← | thiogalactoside acetyltransferase |
RA | 365,783 | T→C | 18.1% | S175G (AGC→GGC) | lacZ ← | beta‑D‑galactosidase |
RA | 421,546 | G→T | 18.7% | L187L (CTG→CTT) | proY → | proline‑specific permease |
RA | 429,749 | A→G | 10.0% | E82G (GAG→GGG) | secF → | SecYEG protein translocase auxillary subunit |
RA | 429,854 | T→G | 15.0% | V117G (GTG→GGG) | secF → | SecYEG protein translocase auxillary subunit |
RA | 435,554 | T→G | 13.7% | *140G (TGA→GGA) | nusB → | transcription antitermination protein |
RA | 469,266 | A→G | 13.2% | G132G (GGA→GGG) | mdlA → | putative multidrug ABC transporter ATPase |
RA | 489,597 | T→G | 13.2% | V1021G (GTG→GGG) | mscK → | mechanosensitive channel protein, intermediate conductance, K+ regulated |
RA | 490,367 | A→C | 14.0% | V149G (GTG→GGG) | priC ← | primosomal replication protein N'' |
RA | 501,710 | T→C | 16.0% | E510G (GAG→GGG) | ybaL ← | inner membrane putative NAD(P)‑binding transporter |
RA | 520,534 | A→G | 24.8% | E40G (GAG→GGG) | ybbP → | putative ABC transporter permease |
RA | 541,102 | A→T | 14.5% | M180L (ATG→TTG) | ybbY → | putative uracil/xanthine transporter |
RA | 542,105 | T→G | 21.2% | V73G (GTG→GGG) | glxK → | glycerate kinase II |
RA | 559,176 | T→A | 17.8% | V68E (GTA→GAA) | sfmC → | putative periplasmic pilus chaperone |
RA | 629,732 | A→G | 9.8% | intergenic (+19/‑162) | entH → / → cstA | enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization, APC peptide transporter family protein |
RA | 630,187 | A→G | 20.1% | G98G (GGA→GGG) | cstA → | carbon starvation protein involved in peptide utilization, APC peptide transporter family protein |
RA | 635,136 | C→T | 11.5% | E81K (GAA→AAA) | ybdM ← | Spo0J family protein, ParB‑like nuclease domain |
RA | 644,291 | Δ1 bp | 3.0% | coding (713/807 nt) | rna ← | ribonuclease I |
RA | 644,428 | A→T | 13.1% | G192G (GGT→GGA) | rna ← | ribonuclease I |
RA | 645,919 | T→C | 9.2% | E221G (GAG→GGG) | citT ← | citrate/succinate antiporter, citrate carrier |
RA | 647,210 | A→C | 26.2% | G100G (GGT→GGG) | citG ← | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
RA | 665,104 | A→C | 15.3% | S29R (AGT→AGG) | rlpA ← | septal ring protein, suppressor of prc, minor lipoprotein |
RA | 695,546 | A→G | 12.8% | E149G (GAG→GGG) | ubiF → | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
RA | 701,419 | C→G | 10.3% | R59P (CGC→CCC) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
RA | 759,427 | A→G | 13.3% | E241G (GAG→GGG) | sucA → | 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding |
RA | 761,097 | A→C | 16.2% | T798P (ACC→CCC) | sucA → | 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding |
RA | 762,113 | T→C | 23.7% | S198P (TCC→CCC) | sucB → | dihydrolipoyltranssuccinase |
RA | 786,855 | A→C | 16.3% | G247G (GGT→GGG) | gpmA ← | phosphoglyceromutase 1 |
RA | 830,872 | A→T | 12.3% | M1M (ATG→TTG) † | rhlE → | ATP‑dependent RNA helicase |
RA | 838,908 | T→G | 6.7% | A100A (GCA→GCC) ‡ | ybiX ← | Fe(II)‑dependent oxygenase superfamily protein |
RA | 838,910 | C→A | 6.1% | A100S (GCA→TCA) ‡ | ybiX ← | Fe(II)‑dependent oxygenase superfamily protein |
RA | 853,476 | A→G | 13.9% | G98G (GGA→GGG) | mntR → | Mn(2+)‑responsive manganese regulon transcriptional regulator |
RA | 897,242 | T→G | 10.4% | G53G (GGT→GGG) | potI → | putrescine ABC transporter permease |
RA | 909,181 | A→G | 14.7% | A38A (GCT→GCC) | ltaE ← | L‑allo‑threonine aldolase, PLP‑dependent |
RA | 921,692 | T→G | 14.4% | V449G (GTG→GGG) | macB → | macrolide ABC transporter peremase/ATPase |
RA | 976,672 | T→G | 17.0% | V116G (GTG→GGG) | mukB → | chromosome condensin MukBEF, ATPase and DNA‑binding subunit |
RA | 1,024,846 | T→C | 14.9% | S126P (TCT→CCT) | helD → | DNA helicase IV |
RA | 1,058,486 | T→C | 14.6% | S135P (TCG→CCG) | torC → | trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit |
RA | 1,058,501 | T→C | 14.8% | S140P (TCC→CCC) | torC → | trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit |
RA | 1,068,572 | G→C | 16.0% | A423G (GCG→GGG) | rutG ← | pyrimidine permease |
RA | 1,069,065 | G→A | 19.4% | L259L (CTG→TTG) | rutG ← | pyrimidine permease |
RA | 1,104,208 | A→G | 17.9% | G86G (GGA→GGG) | csgB → | curlin nucleator protein, minor subunit in curli complex |
RA | 1,158,048 | A→C | 24.8% | P60P (CCA→CCC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
RA | 1,159,550 | T→G | 15.6% | T668P (ACC→CCC) | fhuE ← | ferric‑rhodotorulic acid outer membrane transporter |
RA | 1,170,692 | C→T | 13.5% | E1091E (GAG→GAA) | mfd ← | transcription‑repair coupling factor |
JC | 1,195,194 | Δ3 bp | 4.0% | coding (72‑74/1251 nt) | icd → | isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase |
RA | 1,196,131 | G→A | 29.5% | A337T (GCC→ACC) | icd → | isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase |
RA | 1,196,305 | C→T | 40.9% | R395C (CGT→TGT) | icd → | isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase |
RA | 1,237,805 | C→T | 9.9% | L79F (CTC→TTC) | dadA → | D‑amino acid dehydrogenase |
RA | 1,244,898 | T→G | 18.3% | S57R (AGT→AGG) | ymgE → | UPF0410 family putative inner membrane protein |
RA | 1,264,786 | A→G | 16.5% | E358G (GAG→GGG) | hemA → | glutamyl tRNA reductase |
RA | 1,290,375 | A→G | 14.5% | E45G (GAG→GGG) | rssB → | PcnB‑degradosome interaction factor, response regulator |
RA | 1,295,137 | T→C | 10.6% | pseudogene (5/150 nt) | insZ ← | pseudogene, transposase homolog |
RA | 1,340,431 | C→T | 18.6% | F63F (TTC→TTT) | yciS → | DUF1049 family inner membrane protein, function unknown |
RA | 1,383,219 | A→C | 21.7% | V248G (GTG→GGG) | ycjW ← | LacI family putative transcriptional repressor |
RA | 1,406,283 | T→G | 19.4% | V102G (GTG→GGG) | smrA → | DNA endonuclease |
RA | 1,455,879 | T→C | 17.3% | L239P (CTC→CCC) | paaC → | ring 1,2‑phenylacetyl‑CoA epoxidase subunit |
RA | 1,518,460 | A→G | 4.8% | H129H (CAT→CAC) | mnaT ← | methionine N‑acyltransferase, L‑amino acid N‑acyltransferase |
RA | 1,538,334 | T→G | 15.3% | T174P (ACC→CCC) | narY ← | nitrate reductase 2 (NRZ), beta subunit |
RA | 1,708,844 | C→G | 20.7% | A642A (GCC→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex |
RA | 1,722,415 | A→G | 11.8% | S99G (AGC→GGC) | ydhK → | putative efflux protein (PET) component of YdhJK efflux pump |
RA | 1,727,771 | C→G | 16.1% | intergenic (+15/‑66) | nemA → / → gloA | chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent |
RA | 1,761,252 | A→C | 16.2% | G180G (GGT→GGG) | sufD ← | component of SufBCD Fe‑S cluster assembly scaffold |
RA | 1,825,257 | C→T | 14.0% | Q123Q (CAG→CAA) | spy ← | periplasmic ATP‑independent protein refolding chaperone, stress‑induced |
RA | 1,829,873 | T→G | 20.8% | H298P (CAC→CCC) | astA ← | arginine succinyltransferase |
RA | 1,837,435 | T→G | 23.5% | V75G (GTG→GGG) | ynjC → | putative ABC transporter permease |
RA | 1,839,222 | A→G | 11.0% | E159G (GAG→GGG) | ynjD → | putative ABC transporter ATPase |
RA | 1,860,118 | T→C | 10.3% | S38G (AGT→GGT) | ydjK ← | putative MFS sugar transporter, membrane protein |
RA | 1,861,172 | T→C | 7.6% | E54G (GAG→GGG) | ydjL ← | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
RA | 1,872,402 | T→G | 12.3% | V121G (GTG→GGG) | yeaJ → | putative diguanylate cyclase |
RA | 1,901,015 | T→C | 21.5% | S191G (AGC→GGC) | yoaE ← | putative membrane protein/conserved protein |
RA | 1,976,691 | T→C | 12.9% | E150G (GAG→GGG) | motA ← | proton conductor component of flagella motor |
RA | 2,008,355 | C→T | 19.9% | L27F (CTC→TTC) | yedE → | UPF0394 family sulphur transport domain‑containing inner membrane protein |
RA | 2,029,277 | C→T | 14.7% | A30A (GCG→GCA) | yedI ← | DUF808 family inner membrane protein |
RA | 2,093,736 | T→G | 21.0% | V90G (GTG→GGG) | hisB → | histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
RA | 2,120,281 | T→C | 9.3% | E425G (GAG→GGG) | wcaJ ← | colanic biosynthesis UDP‑glucose lipid carrier transferase |
RA | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,171,546 | G→A | 12.9% | pseudogene (182/299 nt) | gatR ← | pseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted |
RA | 2,181,415 | T→G | 15.5% | C108G (TGT→GGT) | yegV → | putative kinase |
RA | 2,206,915 | T→G | 13.3% | V408G (GTG→GGG) | yehM → | uncharacterized protein |
RA | 2,273,883 | A→C | 12.2% | T508P (ACT→CCT) | yejA → | microcin C ABC transporter periplasmic binding protein |
RA | 2,274,347 | T→G | 24.8% | G57G (GGT→GGG) | yejB → | microcin C ABC transporter permease |
RA | 2,401,838 | A→C | 17.2% | G71G (GGT→GGG) | nuoE ← | NADH:ubiquinone oxidoreductase, chain E |
RA | 2,489,485 | T→G | 17.9% | T234P (ACC→CCC) | yfdV ← | putative transporter |
RA | 2,508,134 | A→C | 21.9% | V36G (GTG→GGG) | fryB ← | putative enzyme IIB component of PTS |
RA | 2,511,426 | G→C | 15.8% | intergenic (+101/+40) | ypeC → / ← mntH | DUF2502 family putative periplasmic protein/manganese/divalent cation transporter |
RA | 2,563,177 | T→C | 17.9% | intergenic (+62/‑413) | yffP → / → yffQ | CPZ‑55 prophage, uncharacterized protein/CPZ‑55 prophage, uncharacterized protein |
RA | 2,733,853 | A→G | 10.5% | S107P (TCG→CCG) | clpB ← | protein disaggregation chaperone |
RA | 2,745,087 | A→C | 16.4% | V84G (GTG→GGG) | trmD ← | tRNA m(1)G37 methyltransferase, SAM‑dependent |
RA | 2,747,957 | A→C | 12.7% | intergenic (‑164/‑3) | ffh ← / → ypjD | Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)/cytochrome c assembly protein family inner membrane protein |
RA | 2,776,305 | A→C | 11.5% | H130P (CAC→CCC) | yfjX → | CP4‑57 prophage, putative antirestriction protein |
RA | 2,782,507 | A→C | 17.8% | V73G (GTA→GGA) | ypjA ← | adhesin‑like autotransporter |
RA | 2,820,041 | T→C | 14.7% | S657G (AGC→GGC) | alaS ← | alanyl‑tRNA synthetase |
RA | 2,828,504 | T→G | 29.5% | V257G (GTG→GGG) | srlD → | sorbitol‑6‑phosphate dehydrogenase |
RA | 2,849,846 | A→C | 13.9% | M1G (GTG→GGG) † | hycB ← | hydrogenase 3, Fe‑S subunit |
RA | 2,872,595 | A→G | 15.1% | L75P (CTC→CCC) | ftsB ← | cell division protein |
RA | 2,876,557 | G→T | 22.6% | intergenic (‑230/‑22) | cysD ← / → iap | sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion |
RA | 2,877,025 | T→G | 20.7% | G149G (GGT→GGG) | iap → | aminopeptidase in alkaline phosphatase isozyme conversion |
RA | 2,909,227 | A→C | 18.4% | G146G (GGT→GGG) | pyrG ← | CTP synthetase |
RA | 2,931,393 | T→G | 20.1% | V114G (GTG→GGG) | ygdG → | Ssb‑binding protein, misidentified as ExoIX |
RA | 2,933,322 | G→A | 11.0% | S122F (TCT→TTT) | fucA ← | L‑fuculose‑1‑phosphate aldolase |
RA | 2,936,758 | T→C | 12.3% | S393P (TCC→CCC) | fucI → | L‑fucose isomerase |
RA | 2,974,291 | A→C | 9.1% | G574G (GGT→GGG) | aas ← | fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase |
RA | 2,999,608 | A→T | 16.7% | R94R (CGT→CGA) | ygeR ← | LysM domain‑containing M23 family putative peptidase, septation lipoprotein |
RA | 3,012,217 | T→G | 13.1% | S253R (AGT→AGG) | yqeA → | putative amino acid kinase |
RA | 3,027,729 | A→G | 16.4% | E204G (GAG→GGG) | ghxQ → | guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter |
RA | 3,037,954 | A→C | 3.3% | W51G (TGG→GGG) | recJ ← | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
RA | 3,064,795 | T→A | 15.2% | intergenic (+19/‑5) | scpB → / → scpC | methylmalonyl‑CoA decarboxylase, biotin‑independent/propionyl‑CoA:succinate CoA transferase |
RA | 3,064,816 | C→A | 18.5% | T6K (ACA→AAA) | scpC → | propionyl‑CoA:succinate CoA transferase |
RA | 3,064,917 | G→C | 14.2% | A40P (GCG→CCG) | scpC → | propionyl‑CoA:succinate CoA transferase |
RA | 3,080,042 | T→C | 9.0% | E531G (GAG→GGG) | tktA ← | transketolase 1, thiamine triphosphate‑binding |
RA | 3,090,128 | A→C | 26.6% | T126P (ACC→CCC) | yggI → | Zn‑dependent metalloprotease‑related protein |
RA | 3,107,793 | A→C | 18.3% | V454G (GTG→GGG) | speC ← | ornithine decarboxylase, constitutive |
RA | 3,113,955 | T→A | 12.6% | L132F (TTA→TTT) | pppA ← | bifunctional prepilin leader peptidase/ methylase |
RA | 3,135,398 | G→T | 11.7% | G324G (GGC→GGA) | pitB ← | phosphate transporter |
RA | 3,141,145 | T→C | 15.0% | E47G (GAG→GGG) | hybD ← | maturation protease for hydrogenase 2 |
RA | 3,145,641 | T→G | 15.5% | T207P (ACT→CCT) | hybO ← | hydrogenase 2, small subunit |
RA | 3,154,040 | T→G | 13.3% | S160R (AGT→AGG) | yghB → | general envelope maintenance protein, DedA family inner membrane protein, putative multidrug efflux transporter |
RA | 3,196,624 | A→C | 15.1% | V43G (GTG→GGG) | hldE ← | heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
RA | 3,216,973 | T→G | 17.8% | V66G (GTG→GGG) | yqjI → | PadR family putative transcriptional regulator |
RA | 3,257,799 | G→A | 10.2% | L70L (CTC→CTT) | yhaO ← | putative transporter |
RA | 3,258,527 | T→C | 9.9% | E374G (GAG→GGG) | tdcG ← | L‑serine dehydratase 3, anaerobic |
RA | 3,265,358 | T→C | 12.1% | G223G (GGA→GGG) | tdcB ← | L‑threonine dehydratase, catabolic |
RA | 3,266,699 | A→C | 17.0% | V122G (GTG→GGG) | tdcA ← | tdc operon transcriptional activator |
RA | 3,277,498 | T→G | 16.4% | V55G (GTG→GGG) | yhaV → | toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system |
RA | 3,277,507 | A→G | 14.9% | E58G (GAG→GGG) | yhaV → | toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system |
RA | 3,289,076 | C→A | 19.5% | Q89K (CAG→AAG) | yraJ → | putative outer membrane protein |
RA | 3,293,729 | C→G | 13.6% | A111G (GCG→GGG) | lpoA → | OM lipoprotein stimulator of MrcA transpeptidase |
RA | 3,293,775 | A→C | 14.1% | T126T (ACA→ACC) | lpoA → | OM lipoprotein stimulator of MrcA transpeptidase |
RA | 3,340,822 | A→T | 15.5% | I184F (ATC→TTC) | yrbG → | putative calcium/sodium:proton antiporter |
RA | 3,388,174 | A→G | 14.6% | S5P (TCC→CCC) | aaeB ← | p‑hydroxybenzoic acid efflux system component |
RA | 3,393,677 | A→T | 12.3% | S860R (AGT→AGA) | yhdP ← | DUF3971‑AsmA2 domains protein |
RA | 3,398,906 | A→G | 17.4% | A357A (GCT→GCC) | mreC ← | cell wall structural complex MreBCD transmembrane component MreC |
RA | 3,416,415 | C→T | 14.3% | S462F (TCT→TTT) | acrF → | multidrug efflux system protein |
RA | 3,417,247 | T→G | 15.3% | G739G (GGT→GGG) | acrF → | multidrug efflux system protein |
RA | 3,444,063 | A→C | 12.6% | S11R (AGT→AGG) | secY ← | preprotein translocase membrane subunit |
RA | 3,460,836 | T→C | 11.4% | L341P (CTC→CCC) | gspF → | general secretory pathway component, cryptic |
RA | 3,479,970 | G→C | 20.2% | G212G (GGC→GGG) | kefB ← | potassium:proton antiporter |
RA | 3,489,860 | A→G | 13.5% | S107P (TCC→CCC) | argD ← | bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase |
RA | 3,501,585 | A→G | 12.8% | E107G (GAG→GGG) | frlB → | fructoselysine‑6‑P‑deglycase |
RA | 3,511,158 | A→C | 11.1% | V88G (GTG→GGG) | yhfY ← | PRD domain protein |
RA | 3,515,929 | A→C | 12.2% | intergenic (‑18/+89) | dam ← / ← damX | DNA adenine methyltransferase/cell division protein that binds to the septal ring |
RA | 3,532,593 | C→A | 20.4% | intergenic (‑156/‑223) | yhgE ← / → pck | DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP] |
RA | 3,534,949 | A→C | 16.3% | G306G (GGT→GGG) | envZ ← | sensory histidine kinase in two‑component regulatory system with OmpR |
RA | 3,544,444 | A→C | 16.7% | S133R (AGT→AGG) | bioH ← | pimeloyl‑ACP methyl ester carboxylesterase |
RA | 3,560,493 | G→A | 6.0% | pseudogene (111/149 nt) | glpR ← | pseudogene, DNA‑binding transcriptional repressor,regulator, Energy metabolism, carbon: Anaerobic respiration, repressor of the glp operon |
RA | 3,565,286 | T→G | 10.9% | H432P (CAC→CCC) | glgP ← | glycogen phosphorylase |
RA | 3,596,135 | T→C | 13.6% | S90G (AGT→GGT) | livH ← | branched‑chain amino acid ABC transporter permease |
RA | 3,638,184 | A→G | 18.0% | S182G (AGC→GGC) | pitA → | phosphate transporter, low‑affinity, tellurite importer |
RA | 3,661,662 | A→G | 16.9% | E417G (GAG→GGG) | mdtF → | anaerobic multidrug efflux transporter, ArcA‑regulated |
RA | 3,661,664 | C→G | 14.7% | R418G (CGT→GGT) | mdtF → | anaerobic multidrug efflux transporter, ArcA‑regulated |
RA | 3,665,654 | T→A | 15.6% | V52V (GTA→GTT) | gadX ← | acid resistance regulon transcriptional activator, autoactivator |
RA | 3,674,184 | T→G | 12.8% | V275G (GTG→GGG) | yhjD → | inner membrane putative BrbK family alternate lipid exporter |
RA | 3,680,184 | T→G | 23.6% | V256G (GTG→GGG) | kdgK → | 2‑dehydro‑3‑deoxygluconokinase |
RA | 3,680,531 | A→G | 11.4% | I470T (ATC→ACC) | yhjJ ← | putative periplasmic M16 family chaperone |
RA | 3,689,746 | T→C | 16.5% | E172G (GAG→GGG) | bcsZ ← | endo‑1,4‑D‑glucanase |
RA | 3,692,311 | T→C | 8.4% | E99G (GAG→GGG) | bcsB ← | regulator of cellulose synthase, cyclic di‑GMP binding |
RA | 3,712,473 | A→G | 6.1% | L154P (CTC→CCC) | yhjY ← | autotransporter beta‑domain protein |
RA | 3,762,307 | A→G | 29.4% | G42G (GGA→GGG) | rhsA → | Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor |
RA | 3,777,790 | A→G | 13.4% | G131G (GGA→GGG) | lldP → | L‑lactate permease |
RA | 3,792,339 | G→A | 8.9% | S71F (TCT→TTT) | kbl ← | glycine C‑acetyltransferase |
RA | 3,798,621 | C→A | 10.6% | V231F (GTT→TTT) | waaU ← | lipopolysaccharide core biosynthesis |
MC JC | 3,815,858 | Δ82 bp | 100% | [rph]–[rph] | [rph], [rph] | |
RA | 3,820,324 | T→C | 14.1% | R282R (CGA→CGG) | ligB ← | DNA ligase, NAD(+)‑dependent |
RA | 3,826,737 | A→G | 11.1% | E510G (GAG→GGG) | recG → | ATP‑dependent DNA helicase |
RA | 3,826,834 | T→G | 15.4% | G542G (GGT→GGG) | recG → | ATP‑dependent DNA helicase |
RA | 3,838,901 | C→T | 15.0% | L219L (CTG→TTG) | yicL → | EamA family inner membrane putative transporter |
RA | 3,845,038 | T→G | 17.9% | V359G (GTG→GGG) | adeD → | cryptic adenine deaminase |
RA | 3,852,480 | A→G | 18.6% | S102P (TCC→CCC) | ilvB ← | acetolactate synthase 2 large subunit |
RA | 3,852,875 | T→G | 13.2% | intergenic (‑92/+14) | ilvB ← / ← ivbL | acetolactate synthase 2 large subunit/ilvB operon leader peptide |
RA | 3,862,669 | T→A | 33.7% | pseudogene (934/1617 nt) | glvC ← | pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component |
RA | 3,873,621 | T→C | 11.3% | S285G (AGC→GGC) | dgoK ← | 2‑oxo‑3‑deoxygalactonate kinase |
RA | 3,877,256 | A→G | 7.4% | L70P (CTC→CCC) | yidB ← | DUF937 family protein |
RA | 3,901,831 | T→A | 12.2% | Y130F (TAT→TTT) | bglH ← | carbohydrate‑specific outer membrane porin, cryptic |
RA | 3,915,852 | T→G | 15.3% | A40A (GCA→GCC) | atpC ← | F1 sector of membrane‑bound ATP synthase, epsilon subunit |
RA | 3,919,589 | A→C | 10.2% | C90G (TGT→GGT) | atpA ← | F1 sector of membrane‑bound ATP synthase, alpha subunit |
RA | 3,932,227 | A→T | 10.6% | I305F (ATC→TTC) | kup → | potassium transporter |
RA | 3,978,861 | A→C | 15.8% | T88P (ACC→CCC) | wzyE → | putative ECA polysaccharide chain elongation protein |
RA | 3,979,401 | A→C | 7.1% | T268P (ACC→CCC) | wzyE → | putative ECA polysaccharide chain elongation protein |
RA | 3,992,449 | T→G | 13.0% | V433G (GTG→GGG) | cyaA → | adenylate cyclase |
RA | 3,993,352 | A→G | 14.9% | D734G (GAC→GGC) | cyaA → | adenylate cyclase |
JC | 4,001,644 | Δ5 bp | 97.3% | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,010,602 | A→G | 7.3% | E135G (GAG→GGG) | yigL → | pyridoxal phosphate phosphatase |
RA | 4,011,446 | T→G | 17.4% | G124G (GGT→GGG) | yigM → | putative inner membrane EamA‑like transporter |
RA | 4,015,350 | T→C | 11.2% | intergenic (+37/+3) | metE → / ← ysgA | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase |
RA | 4,017,124 | A→G | 8.7% | E232G (GAG→GGG) | udp → | uridine phosphorylase |
RA | 4,054,191 | T→G | 10.0% | T363P (ACC→CCC) | glnG ← | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
RA | 4,065,547 | T→A | 11.6% | L73F (TTA→TTT) | yihO ← | putative sulphoquinovose importer |
RA | 4,065,570 | T→A | 16.7% | T66S (ACC→TCC) | yihO ← | putative sulphoquinovose importer |
RA | 4,065,579 | T→A | 23.9% | T63S (ACC→TCC) | yihO ← | putative sulphoquinovose importer |
RA | 4,065,590 | G→C | 14.5% | A59G (GCG→GGG) | yihO ← | putative sulphoquinovose importer |
RA | 4,065,595 | C→A | 19.1% | L57L (CTG→CTT) | yihO ← | putative sulphoquinovose importer |
RA | 4,065,603 | T→A | 26.8% | I55F (ATC→TTC) | yihO ← | putative sulphoquinovose importer |
RA | 4,066,183 | G→A | 8.6% | F337F (TTC→TTT) | yihP ← | putative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein |
RA | 4,069,799 | G→T | 12.0% | T201K (ACA→AAA) | yihR ← | putative sulphoquinovose mutarotase |
RA | 4,093,652 | A→C | 10.7% | V207G (GTG→GGG) | rhaD ← | rhamnulose‑1‑phosphate aldolase |
RA | 4,103,050 | A→G | 10.4% | E77G (GAG→GGG) | yiiM → | 6‑N‑hydroxylaminopurine resistance protein |
RA | 4,103,606 | T→C | 13.4% | S457G (AGT→GGT) | cpxA ← | sensory histidine kinase in two‑component regulatory system with CpxR |
RA | 4,109,839 | A→T | 11.5% | intergenic (+17/‑90) | sbp → / → cdh | sulfate transporter subunit/CDP‑diacylglycerol phosphotidylhydrolase |
RA | 4,122,730 | A→G | 9.2% | S203S (TCT→TCC) | ftsN ← | essential cell division protein |
RA | 4,137,349 | T→C | 10.4% | E103G (GAG→GGG) | yijF ← | DUF1287 family protein |
RA | 4,138,036 | A→C | 15.4% | G333G (GGT→GGG) | gldA ← | glycerol dehydrogenase, NAD+ dependent, 1,2‑propanediol:NAD+ oxidoreductase |
RA | 4,141,475 | A→G | 9.5% | L249P (CTC→CCC) | frwA ← | putative PTS enzyme: Hpr, enzyme I and II components |
RA | 4,142,657 | C→G | 16.7% | G43G (GGC→GGG) | frwC → | putative enzyme IIC component of PTS |
RA | 4,146,771 | T→G | 10.0% | V172G (GTG→GGG) | pflC → | putative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme |
RA | 4,156,160 | A→G | 14.0% | E50G (GAG→GGG) | argB → | acetylglutamate kinase |
RA | 4,156,549 | T→C | 13.0% | S180P (TCC→CCC) | argB → | acetylglutamate kinase |
RA | 4,158,652 | T→G | 12.8% | V55G (GTG→GGG) | oxyR → | oxidative and nitrosative stress transcriptional regulator |
RA | 4,160,853 | C→T | 3.4% | intergenic (‑64/‑270) | sthA ← / → fabR | pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB |
RA | 4,184,542 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,219,538 | C→T | 13.8% | A315V (GCG→GTG) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
RA | 4,241,837 | G→C | 6.8% | G139G (GGC→GGG) | xylE ← | D‑xylose transporter |
RA | 4,241,926 | T→A | 11.8% | T110S (ACC→TCC) | xylE ← | D‑xylose transporter |
RA | 4,241,993 | T→G | 17.5% | S87S (TCA→TCC) | xylE ← | D‑xylose transporter |
RA | 4,258,626 | A→C | 17.8% | T296P (ACC→CCC) | dinF → | oxidative stress resistance protein, putative MATE family efflux pump, UV and mitomycin C inducible protein |
RA | 4,264,147 | C→T | 10.2% | E28E (GAG→GAA) ‡ | qorA ← | quinone oxidoreductase, NADPH‑dependent |
RA | 4,264,148 | T→C | 7.1% | E28G (GAG→GGG) ‡ | qorA ← | quinone oxidoreductase, NADPH‑dependent |
RA | 4,269,659 | C→G | 19.9% | R83G (CGC→GGC) | aphA → | acid phosphatase/phosphotransferase, class B, non‑specific |
RA | 4,284,276 | Δ1 bp | 3.1% | coding (626/1650 nt) | actP ← | acetate transporter |
RA | 4,285,483 | A→G | 12.7% | S630P (TCG→CCG) | acs ← | acetyl‑CoA synthetase |
RA | 4,286,683 | G→C | 6.4% | Q230E (CAG→GAG) | acs ← | acetyl‑CoA synthetase |
RA | 4,318,368 | T→C | 18.2% | E133G (GAG→GGG) | phnK ← | carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein |
RA | 4,368,876 | A→G | 12.9% | E71G (GAG→GGG) | fxsA → | suppressor of F exclusion of phage T7 |
RA | 4,368,931 | C→T | 14.1% | G89G (GGC→GGT) | fxsA → | suppressor of F exclusion of phage T7 |
RA | 4,368,956 | C→T | 13.1% | L98L (CTA→TTA) | fxsA → | suppressor of F exclusion of phage T7 |
RA | 4,377,377 | T→A | 16.1% | Y116F (TAT→TTT) | blc ← | outer membrane lipoprotein cell division and growth lipocalin |
RA | 4,414,921 | T→G | 10.2% | F216V (TTT→GTT) | aidB → | DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase |
RA | 4,430,346 | A→G | 17.7% | E161G (GAG→GGG) | cycA → | D‑alanine/D‑serine/glycine transporter |
RA | 4,434,510 | A→G | 18.6% | intergenic (+16/+113) | ytfH → / ← cpdB | DUF24 family HxlR‑type putative transcriptional regulator/2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
RA | 4,437,249 | A→G | 13.4% | E165G (GAG→GGG) | cysQ → | 3'(2'),5'‑bisphosphate nucleotidase |
RA | 4,444,690 | T→G | 14.0% | V193G (GTG→GGG) | tamB → | translocation and assembly module for autotransporter export, inner membrane subunit |
RA | 4,460,180 | A→G | 12.6% | T62T (ACT→ACC) | nrdG ← | anaerobic ribonucleotide reductase activating protein |
RA | 4,461,970 | A→C | 10.0% | V231G (GTG→GGG) | nrdD ← | anaerobic ribonucleoside‑triphosphate reductase |
RA | 4,462,860 | Δ1 bp | 5.1% | intergenic (‑199/+195) | nrdD ← / ← treC | anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑P hydrolase |
RA | 4,467,784 | G→T | 12.6% | L53F (TTG→TTT) | mgtA → | magnesium transporter |
RA | 4,474,711 | A→G | 14.3% | E196G (GAG→GGG) | bdcR → | transcriptional repressor for divergent bdcA |
RA | 4,480,112 | C→T | 11.7% | L128F (CTC→TTC) | yjgN → | DUF898 family inner membrane protein |
RA | 4,489,850 | A→C | 12.7% | V72G (GTG→GGG) | yjgR ← | DUF853 family protein with NTPase fold |
RA | 4,521,818 | A→C | 10.1% | G68G (GGT→GGG) | yjhF ← | putative transporter |
RA | 4,522,824 | A→G | 10.7% | S424S (TCT→TCC) | yjhG ← | putative dehydratase |
RA | 4,553,236 | T→C | 9.0% | L112P (CTC→CCC) | uxuB → | D‑mannonate oxidoreductase, NAD‑dependent |
RA | 4,556,924 | A→G | 9.0% | intergenic (‑603/‑70) | yjiC ← / → iraD | uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor |
RA | 4,626,669 | A→C | 18.2% | T253P (ACC→CCC) | radA → | DNA repair protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 522888 | 523344 | 457 | 23 [20] | [16] 23 | [rhsD] | [rhsD] |
* | * | ÷ | NC_000913 | 720883 | 721088 | 206 | 22 [18] | [21] 22 | [kdpE] | [kdpE] |
* | * | ÷ | NC_000913 | 1465103 | 1465329 | 227 | 23 [21] | [21] 22 | ynbG | ynbG |
* | * | ÷ | NC_000913 | 2773478 | 2773751 | 274 | 22 [20] | [20] 22 | yfjW | CP4‑57 prophage, putative inner membrane protein |
* | * | ÷ | NC_000913 | 3769565 | 3769974 | 410 | 22 [21] | [21] 23 | [yibV]–[yibU] | [yibV], yibV, [yibU] |
* | * | ÷ | NC_000913 | 4233755 | 4235446 | 1692 | 22 [20] | [21] 22 | pgi | pgi |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 17904 | 130 (1.410) | 4 (0.040) | 4/428 | NT | 3.0% | coding (416/1167 nt) | nhaA | sodium‑proton antiporter |
? | NC_000913 | = 275148 | NA (NA) | intergenic (‑31/‑177) | insH1/mmuP | IS5 transposase and trans‑activator/CP4‑6 prophage, putative S‑methylmethionine transporter | |||||
* | ? | NC_000913 | 257908 = | NA (NA) | 97 (1.050) | 55/430 | NT | 98.0% | intergenic (+9/‑768) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | = 1293039 | 2 (0.020) | intergenic (‑117/‑488) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase | |||||
* | ? | NC_000913 | 458787 = | 53 (0.570) | 35 (0.380) | 29/424 | NT | 40.1% | intergenic (+87/‑101) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | 608011 = | NA (NA) | intergenic (+23/‑54) | hokE/insL1 | toxic polypeptide, small/IS186 transposase | |||||
* | ? | NC_000913 | = 458799 | 47 (0.510) | 38 (0.420) | 32/422 | NT | 45.2% | intergenic (+99/‑89) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | = 609347 | NA (NA) | intergenic (+170/+112) | insL1/entD | IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex | |||||
* | ? | NC_000913 | 838891 = | 93 (1.010) | 5 (0.060) | 4/410 | NT | 5.5% | coding (317/678 nt) | ybiX | Fe(II)‑dependent oxygenase superfamily protein |
? | NC_000913 | 838921 = | 82 (0.930) | coding (287/678 nt) | ybiX | Fe(II)‑dependent oxygenase superfamily protein | |||||
* | ? | NC_000913 | 1207790 = | 15 (0.160) | 66 (0.770) | 51/398 | NT | 75.1% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 30 (0.350) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 13 (0.140) | 93 (1.090) | 58/398 | NT | 81.6% | coding (305/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | = 1209602 | 30 (0.350) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | 1293032 = | 3 (0.030) | 103 (1.110) | 56/430 | NT | 97.2% | intergenic (‑110/‑495) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
? | NC_000913 | = 1979270 | NA (NA) | intergenic (‑55/‑483) | insA/uspC | IS1 repressor TnpA/universal stress protein | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 28 (0.310) | 22/422 | NT | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 1536774 | 66 (0.710) | 3 (0.030) | 3/414 | NT | 3.9% | coding (536/696 nt) | narW | nitrate reductase 2 (NRZ), delta subunit (assembly subunit) |
? | NC_000913 | = 1536833 | 85 (0.950) | coding (477/696 nt) | narW | nitrate reductase 2 (NRZ), delta subunit (assembly subunit) | |||||
* | ? | NC_000913 | = 1978502 | 0 (0.000) | 27 (0.300) | 23/414 | NT | 100% | intergenic (‑305/+16) | flhD/insB1 | flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B |
? | NC_000913 | 1979279 = | 0 (0.000) | intergenic (‑64/‑474) | insA/uspC | IS1 repressor TnpA/universal stress protein | |||||
* | ? | NC_000913 | 1979486 = | 0 (0.000) | 26 (0.280) | 15/430 | NT | 100% | intergenic (‑271/‑267) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
* | ? | NC_000913 | = 1979489 | 0 (0.000) | 57 (0.620) | 40/430 | NT | 100% | intergenic (‑274/‑264) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | = 2290111 | NA (NA) | intergenic (‑32/+1) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system | |||||
* | ? | NC_000913 | 2514275 = | NA (NA) | 9 (0.100) | 8/424 | NT | 10.5% | intergenic (+33/‑54) | nupC/insL1 | nucleoside (except guanosine) transporter/IS186 transposase |
? | NC_000913 | = 2536318 | 78 (0.840) | coding (487/510 nt) | crr | glucose‑specific enzyme IIA component of PTS | |||||
* | ? | NC_000913 | = 2515612 | NA (NA) | 7 (0.080) | 7/426 | NT | 8.1% | intergenic (+171/+29) | insL1/yfeA | IS186 transposase/putative diguanylate cyclase |
? | NC_000913 | 2536308 = | 80 (0.860) | coding (477/510 nt) | crr | glucose‑specific enzyme IIA component of PTS | |||||
* | ? | NC_000913 | = 2670135 | 110 (1.190) | 3 (0.030) | 3/416 | NT | 3.6% | coding (1106/1362 nt) | hcaE | 3‑phenylpropionate dioxygenase, large (alpha) subunit |
? | NC_000913 | = 2670347 | 55 (0.610) | coding (1318/1362 nt) | hcaE | 3‑phenylpropionate dioxygenase, large (alpha) subunit | |||||
* | ? | NC_000913 | 2882232 = | 92 (0.990) | 3 (0.040) | 3/394 | NT | 3.6% | coding (404/483 nt) | casB | CRISP RNA (crRNA) containing Cascade antiviral complex protein |
? | NC_000913 | 2882250 = | 77 (0.910) | coding (386/483 nt) | casB | CRISP RNA (crRNA) containing Cascade antiviral complex protein | |||||
* | ? | NC_000913 | 4160851 = | 185 (2.000) | 8 (0.090) | 8/428 | NT | 4.2% | intergenic (‑62/‑272) | sthA/fabR | pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB |
? | NC_000913 | = 4499511 | NA (NA) | intergenic (+10/+83) | insD1/yjgX | IS2 transposase TnpB/pseudogene fragment |