breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 12,435 C→A 14.1% F91L (TTC→TTA dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 37,255 A→C 17.4% G190G (GGT→GGG caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 62,169 T→C 13.0% S366G (AGC→GGC)  rapA ← RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 71,877 A→G 28.7% E176G (GAG→GGG)  yabI → DedA family inner membrane protein
RA 144,085 A→C 19.4% S128S (TCA→TCC yadH → putative ABC transporter permease
RA 151,757 A→G 8.7% S159P (TCC→CCC)  yadL ← putative fimbrial‑like adhesin protein
RA 203,550 T→G 12.6% V68G (GTG→GGG)  lpxB → tetraacyldisaccharide‑1‑P synthase
RA 207,473 T→G 27.3% M783R (ATG→AGG)  dnaE → DNA polymerase III alpha subunit
RA 216,077 G→C 5.6% intergenic (+98/+102) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
RA 223,706 T→A 7.5% intergenic (+298/‑65) gmhB → / → rrsH D,D‑heptose 1,7‑bisphosphate phosphatase/16S ribosomal RNA of rrnH operon
RA 249,538 T→G 28.1% pseudogene (533/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment, flagellar biosynthesis
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 289,839 T→C 15.2% E156G (GAG→GGG)  argF ← ornithine carbamoyltransferase 2, chain F, CP4‑6 prophage
RA 324,226 A→G 11.0% S157G (AGC→GGC)  ykgG → LutC family protein, putative electron transport chain YkgEFG component
RA 342,454 A→G 13.8% R444G (AGA→GGA)  yahJ → putative metallo‑dependent hydrolase domain deaminase
RA 357,381 T→G 11.3% V404G (GTG→GGG)  codA → cytosine/isoguanine deaminase
RA 361,668 C→T 17.5% E65K (GAA→AAA)  lacA ← thiogalactoside acetyltransferase
RA 361,670 A→T 18.9% V64E (GTA→GAA)  lacA ← thiogalactoside acetyltransferase
RA 365,783 T→C 18.1% S175G (AGC→GGC)  lacZ ← beta‑D‑galactosidase
RA 421,546 G→T 18.7% L187L (CTG→CTT proY → proline‑specific permease
RA 429,749 A→G 10.0% E82G (GAG→GGG)  secF → SecYEG protein translocase auxillary subunit
RA 429,854 T→G 15.0% V117G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit
RA 435,554 T→G 13.7% *140G (TGA→GGA)  nusB → transcription antitermination protein
RA 469,266 A→G 13.2% G132G (GGA→GGG mdlA → putative multidrug ABC transporter ATPase
RA 489,597 T→G 13.2% V1021G (GTG→GGG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated
RA 490,367 A→C 14.0% V149G (GTG→GGG)  priC ← primosomal replication protein N''
RA 501,710 T→C 16.0% E510G (GAG→GGG)  ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 520,534 A→G 24.8% E40G (GAG→GGG)  ybbP → putative ABC transporter permease
RA 541,102 A→T 14.5% M180L (ATG→TTG)  ybbY → putative uracil/xanthine transporter
RA 542,105 T→G 21.2% V73G (GTG→GGG)  glxK → glycerate kinase II
RA 559,176 T→A 17.8% V68E (GTA→GAA)  sfmC → putative periplasmic pilus chaperone
RA 629,732 A→G 9.8% intergenic (+19/‑162) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
RA 630,187 A→G 20.1% G98G (GGA→GGG cstA → carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
RA 635,136 C→T 11.5% E81K (GAA→AAA)  ybdM ← Spo0J family protein, ParB‑like nuclease domain
RA 644,291 Δ1 bp 3.0% coding (713/807 nt) rna ← ribonuclease I
RA 644,428 A→T 13.1% G192G (GGT→GGA rna ← ribonuclease I
RA 645,919 T→C 9.2% E221G (GAG→GGG)  citT ← citrate/succinate antiporter, citrate carrier
RA 647,210 A→C 26.2% G100G (GGT→GGG citG ← 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
RA 665,104 A→C 15.3% S29R (AGT→AGG rlpA ← septal ring protein, suppressor of prc, minor lipoprotein
RA 695,546 A→G 12.8% E149G (GAG→GGG)  ubiF → 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
RA 701,419 C→G 10.3% R59P (CGC→CCC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 759,427 A→G 13.3% E241G (GAG→GGG)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 761,097 A→C 16.2% T798P (ACC→CCC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 762,113 T→C 23.7% S198P (TCC→CCC)  sucB → dihydrolipoyltranssuccinase
RA 786,855 A→C 16.3% G247G (GGT→GGG gpmA ← phosphoglyceromutase 1
RA 830,872 A→T 12.3% M1M (ATG→TTG) † rhlE → ATP‑dependent RNA helicase
RA 838,908 T→G 6.7% A100A (GCA→GCC) ‡ ybiX ← Fe(II)‑dependent oxygenase superfamily protein
RA 838,910 C→A 6.1% A100S (GCA→TCA) ‡ ybiX ← Fe(II)‑dependent oxygenase superfamily protein
RA 853,476 A→G 13.9% G98G (GGA→GGG mntR → Mn(2+)‑responsive manganese regulon transcriptional regulator
RA 897,242 T→G 10.4% G53G (GGT→GGG potI → putrescine ABC transporter permease
RA 909,181 A→G 14.7% A38A (GCT→GCC ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 921,692 T→G 14.4% V449G (GTG→GGG)  macB → macrolide ABC transporter peremase/ATPase
RA 976,672 T→G 17.0% V116G (GTG→GGG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 1,024,846 T→C 14.9% S126P (TCT→CCT)  helD → DNA helicase IV
RA 1,058,486 T→C 14.6% S135P (TCG→CCG)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,058,501 T→C 14.8% S140P (TCC→CCC)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,068,572 G→C 16.0% A423G (GCG→GGG)  rutG ← pyrimidine permease
RA 1,069,065 G→A 19.4% L259L (CTG→TTG)  rutG ← pyrimidine permease
RA 1,104,208 A→G 17.9% G86G (GGA→GGG csgB → curlin nucleator protein, minor subunit in curli complex
RA 1,158,048 A→C 24.8% P60P (CCA→CCC ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,159,550 T→G 15.6% T668P (ACC→CCC)  fhuE ← ferric‑rhodotorulic acid outer membrane transporter
RA 1,170,692 C→T 13.5% E1091E (GAG→GAA mfd ← transcription‑repair coupling factor
JC 1,195,194 Δ3 bp 4.0% coding (72‑74/1251 nt) icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,196,131 G→A 29.5% A337T (GCC→ACC)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,196,305 C→T 40.9% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,237,805 C→T 9.9% L79F (CTC→TTC)  dadA → D‑amino acid dehydrogenase
RA 1,244,898 T→G 18.3% S57R (AGT→AGG ymgE → UPF0410 family putative inner membrane protein
RA 1,264,786 A→G 16.5% E358G (GAG→GGG)  hemA → glutamyl tRNA reductase
RA 1,290,375 A→G 14.5% E45G (GAG→GGG)  rssB → PcnB‑degradosome interaction factor, response regulator
RA 1,295,137 T→C 10.6% pseudogene (5/150 nt) insZ ← pseudogene, transposase homolog
RA 1,340,431 C→T 18.6% F63F (TTC→TTT yciS → DUF1049 family inner membrane protein, function unknown
RA 1,383,219 A→C 21.7% V248G (GTG→GGG)  ycjW ← LacI family putative transcriptional repressor
RA 1,406,283 T→G 19.4% V102G (GTG→GGG)  smrA → DNA endonuclease
RA 1,455,879 T→C 17.3% L239P (CTC→CCC)  paaC → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,518,460 A→G 4.8% H129H (CAT→CAC mnaT ← methionine N‑acyltransferase, L‑amino acid N‑acyltransferase
RA 1,538,334 T→G 15.3% T174P (ACC→CCC)  narY ← nitrate reductase 2 (NRZ), beta subunit
RA 1,708,844 C→G 20.7% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,722,415 A→G 11.8% S99G (AGC→GGC)  ydhK → putative efflux protein (PET) component of YdhJK efflux pump
RA 1,727,771 C→G 16.1% intergenic (+15/‑66) nemA → / → gloA chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent
RA 1,761,252 A→C 16.2% G180G (GGT→GGG sufD ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,825,257 C→T 14.0% Q123Q (CAG→CAA spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced
RA 1,829,873 T→G 20.8% H298P (CAC→CCC)  astA ← arginine succinyltransferase
RA 1,837,435 T→G 23.5% V75G (GTG→GGG)  ynjC → putative ABC transporter permease
RA 1,839,222 A→G 11.0% E159G (GAG→GGG)  ynjD → putative ABC transporter ATPase
RA 1,860,118 T→C 10.3% S38G (AGT→GGT)  ydjK ← putative MFS sugar transporter, membrane protein
RA 1,861,172 T→C 7.6% E54G (GAG→GGG)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,872,402 T→G 12.3% V121G (GTG→GGG)  yeaJ → putative diguanylate cyclase
RA 1,901,015 T→C 21.5% S191G (AGC→GGC)  yoaE ← putative membrane protein/conserved protein
RA 1,976,691 T→C 12.9% E150G (GAG→GGG)  motA ← proton conductor component of flagella motor
RA 2,008,355 C→T 19.9% L27F (CTC→TTC)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,029,277 C→T 14.7% A30A (GCG→GCA yedI ← DUF808 family inner membrane protein
RA 2,093,736 T→G 21.0% V90G (GTG→GGG)  hisB → histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
RA 2,120,281 T→C 9.3% E425G (GAG→GGG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,171,546 G→A 12.9% pseudogene (182/299 nt) gatR ← pseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted
RA 2,181,415 T→G 15.5% C108G (TGT→GGT)  yegV → putative kinase
RA 2,206,915 T→G 13.3% V408G (GTG→GGG)  yehM → uncharacterized protein
RA 2,273,883 A→C 12.2% T508P (ACT→CCT)  yejA → microcin C ABC transporter periplasmic binding protein
RA 2,274,347 T→G 24.8% G57G (GGT→GGG yejB → microcin C ABC transporter permease
RA 2,401,838 A→C 17.2% G71G (GGT→GGG nuoE ← NADH:ubiquinone oxidoreductase, chain E
RA 2,489,485 T→G 17.9% T234P (ACC→CCC)  yfdV ← putative transporter
RA 2,508,134 A→C 21.9% V36G (GTG→GGG)  fryB ← putative enzyme IIB component of PTS
RA 2,511,426 G→C 15.8% intergenic (+101/+40) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter
RA 2,563,177 T→C 17.9% intergenic (+62/‑413) yffP → / → yffQ CPZ‑55 prophage, uncharacterized protein/CPZ‑55 prophage, uncharacterized protein
RA 2,733,853 A→G 10.5% S107P (TCG→CCG)  clpB ← protein disaggregation chaperone
RA 2,745,087 A→C 16.4% V84G (GTG→GGG)  trmD ← tRNA m(1)G37 methyltransferase, SAM‑dependent
RA 2,747,957 A→C 12.7% intergenic (‑164/‑3) ffh ← / → ypjD Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)/cytochrome c assembly protein family inner membrane protein
RA 2,776,305 A→C 11.5% H130P (CAC→CCC)  yfjX → CP4‑57 prophage, putative antirestriction protein
RA 2,782,507 A→C 17.8% V73G (GTA→GGA)  ypjA ← adhesin‑like autotransporter
RA 2,820,041 T→C 14.7% S657G (AGC→GGC)  alaS ← alanyl‑tRNA synthetase
RA 2,828,504 T→G 29.5% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,849,846 A→C 13.9% M1G (GTG→GGG) † hycB ← hydrogenase 3, Fe‑S subunit
RA 2,872,595 A→G 15.1% L75P (CTC→CCC)  ftsB ← cell division protein
RA 2,876,557 G→T 22.6% intergenic (‑230/‑22) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,877,025 T→G 20.7% G149G (GGT→GGG iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,909,227 A→C 18.4% G146G (GGT→GGG pyrG ← CTP synthetase
RA 2,931,393 T→G 20.1% V114G (GTG→GGG)  ygdG → Ssb‑binding protein, misidentified as ExoIX
RA 2,933,322 G→A 11.0% S122F (TCT→TTT)  fucA ← L‑fuculose‑1‑phosphate aldolase
RA 2,936,758 T→C 12.3% S393P (TCC→CCC)  fucI → L‑fucose isomerase
RA 2,974,291 A→C 9.1% G574G (GGT→GGG aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
RA 2,999,608 A→T 16.7% R94R (CGT→CGA ygeR ← LysM domain‑containing M23 family putative peptidase, septation lipoprotein
RA 3,012,217 T→G 13.1% S253R (AGT→AGG yqeA → putative amino acid kinase
RA 3,027,729 A→G 16.4% E204G (GAG→GGG)  ghxQ → guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter
RA 3,037,954 A→C 3.3% W51G (TGG→GGG)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,064,795 T→A 15.2% intergenic (+19/‑5) scpB → / → scpC methylmalonyl‑CoA decarboxylase, biotin‑independent/propionyl‑CoA:succinate CoA transferase
RA 3,064,816 C→A 18.5% T6K (ACA→AAA)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,064,917 G→C 14.2% A40P (GCG→CCG)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,080,042 T→C 9.0% E531G (GAG→GGG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,090,128 A→C 26.6% T126P (ACC→CCC)  yggI → Zn‑dependent metalloprotease‑related protein
RA 3,107,793 A→C 18.3% V454G (GTG→GGG)  speC ← ornithine decarboxylase, constitutive
RA 3,113,955 T→A 12.6% L132F (TTA→TTT pppA ← bifunctional prepilin leader peptidase/ methylase
RA 3,135,398 G→T 11.7% G324G (GGC→GGA pitB ← phosphate transporter
RA 3,141,145 T→C 15.0% E47G (GAG→GGG)  hybD ← maturation protease for hydrogenase 2
RA 3,145,641 T→G 15.5% T207P (ACT→CCT)  hybO ← hydrogenase 2, small subunit
RA 3,154,040 T→G 13.3% S160R (AGT→AGG yghB → general envelope maintenance protein, DedA family inner membrane protein, putative multidrug efflux transporter
RA 3,196,624 A→C 15.1% V43G (GTG→GGG)  hldE ← heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
RA 3,216,973 T→G 17.8% V66G (GTG→GGG)  yqjI → PadR family putative transcriptional regulator
RA 3,257,799 G→A 10.2% L70L (CTC→CTT yhaO ← putative transporter
RA 3,258,527 T→C 9.9% E374G (GAG→GGG)  tdcG ← L‑serine dehydratase 3, anaerobic
RA 3,265,358 T→C 12.1% G223G (GGA→GGG tdcB ← L‑threonine dehydratase, catabolic
RA 3,266,699 A→C 17.0% V122G (GTG→GGG)  tdcA ← tdc operon transcriptional activator
RA 3,277,498 T→G 16.4% V55G (GTG→GGG)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
RA 3,277,507 A→G 14.9% E58G (GAG→GGG)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
RA 3,289,076 C→A 19.5% Q89K (CAG→AAG)  yraJ → putative outer membrane protein
RA 3,293,729 C→G 13.6% A111G (GCG→GGG)  lpoA → OM lipoprotein stimulator of MrcA transpeptidase
RA 3,293,775 A→C 14.1% T126T (ACA→ACC lpoA → OM lipoprotein stimulator of MrcA transpeptidase
RA 3,340,822 A→T 15.5% I184F (ATC→TTC)  yrbG → putative calcium/sodium:proton antiporter
RA 3,388,174 A→G 14.6% S5P (TCC→CCC)  aaeB ← p‑hydroxybenzoic acid efflux system component
RA 3,393,677 A→T 12.3% S860R (AGT→AGA yhdP ← DUF3971‑AsmA2 domains protein
RA 3,398,906 A→G 17.4% A357A (GCT→GCC mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,416,415 C→T 14.3% S462F (TCT→TTT)  acrF → multidrug efflux system protein
RA 3,417,247 T→G 15.3% G739G (GGT→GGG acrF → multidrug efflux system protein
RA 3,444,063 A→C 12.6% S11R (AGT→AGG secY ← preprotein translocase membrane subunit
RA 3,460,836 T→C 11.4% L341P (CTC→CCC)  gspF → general secretory pathway component, cryptic
RA 3,479,970 G→C 20.2% G212G (GGC→GGG kefB ← potassium:proton antiporter
RA 3,489,860 A→G 13.5% S107P (TCC→CCC)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
RA 3,501,585 A→G 12.8% E107G (GAG→GGG)  frlB → fructoselysine‑6‑P‑deglycase
RA 3,511,158 A→C 11.1% V88G (GTG→GGG)  yhfY ← PRD domain protein
RA 3,515,929 A→C 12.2% intergenic (‑18/+89) dam ← / ← damX DNA adenine methyltransferase/cell division protein that binds to the septal ring
RA 3,532,593 C→A 20.4% intergenic (‑156/‑223) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]
RA 3,534,949 A→C 16.3% G306G (GGT→GGG envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 3,544,444 A→C 16.7% S133R (AGT→AGG bioH ← pimeloyl‑ACP methyl ester carboxylesterase
RA 3,560,493 G→A 6.0% pseudogene (111/149 nt) glpR ← pseudogene, DNA‑binding transcriptional repressor,regulator, Energy metabolism, carbon: Anaerobic respiration, repressor of the glp operon
RA 3,565,286 T→G 10.9% H432P (CAC→CCC)  glgP ← glycogen phosphorylase
RA 3,596,135 T→C 13.6% S90G (AGT→GGT)  livH ← branched‑chain amino acid ABC transporter permease
RA 3,638,184 A→G 18.0% S182G (AGC→GGC)  pitA → phosphate transporter, low‑affinity, tellurite importer
RA 3,661,662 A→G 16.9% E417G (GAG→GGG)  mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,661,664 C→G 14.7% R418G (CGT→GGT)  mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,665,654 T→A 15.6% V52V (GTA→GTT gadX ← acid resistance regulon transcriptional activator, autoactivator
RA 3,674,184 T→G 12.8% V275G (GTG→GGG)  yhjD → inner membrane putative BrbK family alternate lipid exporter
RA 3,680,184 T→G 23.6% V256G (GTG→GGG)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,680,531 A→G 11.4% I470T (ATC→ACC)  yhjJ ← putative periplasmic M16 family chaperone
RA 3,689,746 T→C 16.5% E172G (GAG→GGG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,692,311 T→C 8.4% E99G (GAG→GGG)  bcsB ← regulator of cellulose synthase, cyclic di‑GMP binding
RA 3,712,473 A→G 6.1% L154P (CTC→CCC)  yhjY ← autotransporter beta‑domain protein
RA 3,762,307 A→G 29.4% G42G (GGA→GGG rhsA → Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
RA 3,777,790 A→G 13.4% G131G (GGA→GGG lldP → L‑lactate permease
RA 3,792,339 G→A 8.9% S71F (TCT→TTT)  kbl ← glycine C‑acetyltransferase
RA 3,798,621 C→A 10.6% V231F (GTT→TTT)  waaU ← lipopolysaccharide core biosynthesis
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,820,324 T→C 14.1% R282R (CGA→CGG ligB ← DNA ligase, NAD(+)‑dependent
RA 3,826,737 A→G 11.1% E510G (GAG→GGG)  recG → ATP‑dependent DNA helicase
RA 3,826,834 T→G 15.4% G542G (GGT→GGG recG → ATP‑dependent DNA helicase
RA 3,838,901 C→T 15.0% L219L (CTG→TTG)  yicL → EamA family inner membrane putative transporter
RA 3,845,038 T→G 17.9% V359G (GTG→GGG)  adeD → cryptic adenine deaminase
RA 3,852,480 A→G 18.6% S102P (TCC→CCC)  ilvB ← acetolactate synthase 2 large subunit
RA 3,852,875 T→G 13.2% intergenic (‑92/+14) ilvB ← / ← ivbL acetolactate synthase 2 large subunit/ilvB operon leader peptide
RA 3,862,669 T→A 33.7% pseudogene (934/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
RA 3,873,621 T→C 11.3% S285G (AGC→GGC)  dgoK ← 2‑oxo‑3‑deoxygalactonate kinase
RA 3,877,256 A→G 7.4% L70P (CTC→CCC)  yidB ← DUF937 family protein
RA 3,901,831 T→A 12.2% Y130F (TAT→TTT)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,915,852 T→G 15.3% A40A (GCA→GCC atpC ← F1 sector of membrane‑bound ATP synthase, epsilon subunit
RA 3,919,589 A→C 10.2% C90G (TGT→GGT)  atpA ← F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 3,932,227 A→T 10.6% I305F (ATC→TTC)  kup → potassium transporter
RA 3,978,861 A→C 15.8% T88P (ACC→CCC)  wzyE → putative ECA polysaccharide chain elongation protein
RA 3,979,401 A→C 7.1% T268P (ACC→CCC)  wzyE → putative ECA polysaccharide chain elongation protein
RA 3,992,449 T→G 13.0% V433G (GTG→GGG)  cyaA → adenylate cyclase
RA 3,993,352 A→G 14.9% D734G (GAC→GGC)  cyaA → adenylate cyclase
JC 4,001,644 Δ5 bp 97.3% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,010,602 A→G 7.3% E135G (GAG→GGG)  yigL → pyridoxal phosphate phosphatase
RA 4,011,446 T→G 17.4% G124G (GGT→GGG yigM → putative inner membrane EamA‑like transporter
RA 4,015,350 T→C 11.2% intergenic (+37/+3) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
RA 4,017,124 A→G 8.7% E232G (GAG→GGG)  udp → uridine phosphorylase
RA 4,054,191 T→G 10.0% T363P (ACC→CCC)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
RA 4,065,547 T→A 11.6% L73F (TTA→TTT yihO ← putative sulphoquinovose importer
RA 4,065,570 T→A 16.7% T66S (ACC→TCC)  yihO ← putative sulphoquinovose importer
RA 4,065,579 T→A 23.9% T63S (ACC→TCC)  yihO ← putative sulphoquinovose importer
RA 4,065,590 G→C 14.5% A59G (GCG→GGG)  yihO ← putative sulphoquinovose importer
RA 4,065,595 C→A 19.1% L57L (CTG→CTT yihO ← putative sulphoquinovose importer
RA 4,065,603 T→A 26.8% I55F (ATC→TTC)  yihO ← putative sulphoquinovose importer
RA 4,066,183 G→A 8.6% F337F (TTC→TTT yihP ← putative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein
RA 4,069,799 G→T 12.0% T201K (ACA→AAA)  yihR ← putative sulphoquinovose mutarotase
RA 4,093,652 A→C 10.7% V207G (GTG→GGG)  rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,103,050 A→G 10.4% E77G (GAG→GGG)  yiiM → 6‑N‑hydroxylaminopurine resistance protein
RA 4,103,606 T→C 13.4% S457G (AGT→GGT)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,109,839 A→T 11.5% intergenic (+17/‑90) sbp → / → cdh sulfate transporter subunit/CDP‑diacylglycerol phosphotidylhydrolase
RA 4,122,730 A→G 9.2% S203S (TCT→TCC ftsN ← essential cell division protein
RA 4,137,349 T→C 10.4% E103G (GAG→GGG)  yijF ← DUF1287 family protein
RA 4,138,036 A→C 15.4% G333G (GGT→GGG gldA ← glycerol dehydrogenase, NAD+ dependent, 1,2‑propanediol:NAD+ oxidoreductase
RA 4,141,475 A→G 9.5% L249P (CTC→CCC)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,142,657 C→G 16.7% G43G (GGC→GGG frwC → putative enzyme IIC component of PTS
RA 4,146,771 T→G 10.0% V172G (GTG→GGG)  pflC → putative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme
RA 4,156,160 A→G 14.0% E50G (GAG→GGG)  argB → acetylglutamate kinase
RA 4,156,549 T→C 13.0% S180P (TCC→CCC)  argB → acetylglutamate kinase
RA 4,158,652 T→G 12.8% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,160,853 C→T 3.4% intergenic (‑64/‑270) sthA ← / → fabR pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,219,538 C→T 13.8% A315V (GCG→GTG)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,241,837 G→C 6.8% G139G (GGC→GGG xylE ← D‑xylose transporter
RA 4,241,926 T→A 11.8% T110S (ACC→TCC)  xylE ← D‑xylose transporter
RA 4,241,993 T→G 17.5% S87S (TCA→TCC xylE ← D‑xylose transporter
RA 4,258,626 A→C 17.8% T296P (ACC→CCC)  dinF → oxidative stress resistance protein, putative MATE family efflux pump, UV and mitomycin C inducible protein
RA 4,264,147 C→T 10.2% E28E (GAG→GAA) ‡ qorA ← quinone oxidoreductase, NADPH‑dependent
RA 4,264,148 T→C 7.1% E28G (GAG→GGG) ‡ qorA ← quinone oxidoreductase, NADPH‑dependent
RA 4,269,659 C→G 19.9% R83G (CGC→GGC)  aphA → acid phosphatase/phosphotransferase, class B, non‑specific
RA 4,284,276 Δ1 bp 3.1% coding (626/1650 nt) actP ← acetate transporter
RA 4,285,483 A→G 12.7% S630P (TCG→CCG)  acs ← acetyl‑CoA synthetase
RA 4,286,683 G→C 6.4% Q230E (CAG→GAG)  acs ← acetyl‑CoA synthetase
RA 4,318,368 T→C 18.2% E133G (GAG→GGG)  phnK ← carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
RA 4,368,876 A→G 12.9% E71G (GAG→GGG)  fxsA → suppressor of F exclusion of phage T7
RA 4,368,931 C→T 14.1% G89G (GGC→GGT fxsA → suppressor of F exclusion of phage T7
RA 4,368,956 C→T 13.1% L98L (CTA→TTA)  fxsA → suppressor of F exclusion of phage T7
RA 4,377,377 T→A 16.1% Y116F (TAT→TTT)  blc ← outer membrane lipoprotein cell division and growth lipocalin
RA 4,414,921 T→G 10.2% F216V (TTT→GTT)  aidB → DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,430,346 A→G 17.7% E161G (GAG→GGG)  cycA → D‑alanine/D‑serine/glycine transporter
RA 4,434,510 A→G 18.6% intergenic (+16/+113) ytfH → / ← cpdB DUF24 family HxlR‑type putative transcriptional regulator/2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
RA 4,437,249 A→G 13.4% E165G (GAG→GGG)  cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,444,690 T→G 14.0% V193G (GTG→GGG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,460,180 A→G 12.6% T62T (ACT→ACC nrdG ← anaerobic ribonucleotide reductase activating protein
RA 4,461,970 A→C 10.0% V231G (GTG→GGG)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,462,860 Δ1 bp 5.1% intergenic (‑199/+195) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑P hydrolase
RA 4,467,784 G→T 12.6% L53F (TTG→TTT mgtA → magnesium transporter
RA 4,474,711 A→G 14.3% E196G (GAG→GGG)  bdcR → transcriptional repressor for divergent bdcA
RA 4,480,112 C→T 11.7% L128F (CTC→TTC)  yjgN → DUF898 family inner membrane protein
RA 4,489,850 A→C 12.7% V72G (GTG→GGG)  yjgR ← DUF853 family protein with NTPase fold
RA 4,521,818 A→C 10.1% G68G (GGT→GGG yjhF ← putative transporter
RA 4,522,824 A→G 10.7% S424S (TCT→TCC yjhG ← putative dehydratase
RA 4,553,236 T→C 9.0% L112P (CTC→CCC)  uxuB → D‑mannonate oxidoreductase, NAD‑dependent
RA 4,556,924 A→G 9.0% intergenic (‑603/‑70) yjiC ← / → iraD uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor
RA 4,626,669 A→C 18.2% T253P (ACC→CCC)  radA → DNA repair protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 522888 523344 457 23 [20] [16] 23 [rhsD] [rhsD]
* * ÷ NC_000913 720883 721088 206 22 [18] [21] 22 [kdpE] [kdpE]
* * ÷ NC_000913 1465103 1465329 227 23 [21] [21] 22 ynbG ynbG
* * ÷ NC_000913 2773478 2773751 274 22 [20] [20] 22 yfjW CP4‑57 prophage, putative inner membrane protein
* * ÷ NC_000913 3769565 3769974 410 22 [21] [21] 23 [yibV]–[yibU] [yibV], yibV, [yibU]
* * ÷ NC_000913 4233755 4235446 1692 22 [20] [21] 22 pgi pgi

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 17904130 (1.410)4 (0.040) 4/428 NT 3.0% coding (416/1167 nt) nhaA sodium‑proton antiporter
?NC_000913 = 275148 NA (NA)intergenic (‑31/‑177) insH1/mmuP IS5 transposase and trans‑activator/CP4‑6 prophage, putative S‑methylmethionine transporter
* ? NC_000913 257908 =NA (NA)97 (1.050) 55/430 NT 98.0% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 2 (0.020)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 458787 =53 (0.570)35 (0.380) 29/424 NT 40.1% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 608011 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? NC_000913 = 45879947 (0.510)38 (0.420) 32/422 NT 45.2% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 609347 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? NC_000913 838891 =93 (1.010)5 (0.060) 4/410 NT 5.5% coding (317/678 nt) ybiX Fe(II)‑dependent oxygenase superfamily protein
?NC_000913 838921 = 82 (0.930)coding (287/678 nt) ybiX Fe(II)‑dependent oxygenase superfamily protein
* ? NC_000913 1207790 =15 (0.160)66 (0.770) 51/398 NT 75.1% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 30 (0.350)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780513 (0.140)93 (1.090) 58/398 NT 81.6% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 30 (0.350)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =3 (0.030)103 (1.110) 56/430 NT 97.2% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)28 (0.310) 22/422 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 153677466 (0.710)3 (0.030) 3/414 NT 3.9% coding (536/696 nt) narW nitrate reductase 2 (NRZ), delta subunit (assembly subunit)
?NC_000913 = 1536833 85 (0.950)coding (477/696 nt) narW nitrate reductase 2 (NRZ), delta subunit (assembly subunit)
* ? NC_000913 = 19785020 (0.000)27 (0.300) 23/414 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)26 (0.280) 15/430 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)57 (0.620) 40/430 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2514275 =NA (NA)9 (0.100) 8/424 NT 10.5% intergenic (+33/‑54) nupC/insL1 nucleoside (except guanosine) transporter/IS186 transposase
?NC_000913 = 2536318 78 (0.840)coding (487/510 nt) crr glucose‑specific enzyme IIA component of PTS
* ? NC_000913 = 2515612NA (NA)7 (0.080) 7/426 NT 8.1% intergenic (+171/+29) insL1/yfeA IS186 transposase/putative diguanylate cyclase
?NC_000913 2536308 = 80 (0.860)coding (477/510 nt) crr glucose‑specific enzyme IIA component of PTS
* ? NC_000913 = 2670135110 (1.190)3 (0.030) 3/416 NT 3.6% coding (1106/1362 nt) hcaE 3‑phenylpropionate dioxygenase, large (alpha) subunit
?NC_000913 = 2670347 55 (0.610)coding (1318/1362 nt) hcaE 3‑phenylpropionate dioxygenase, large (alpha) subunit
* ? NC_000913 2882232 =92 (0.990)3 (0.040) 3/394 NT 3.6% coding (404/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein
?NC_000913 2882250 = 77 (0.910)coding (386/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein
* ? NC_000913 4160851 =185 (2.000)8 (0.090) 8/428 NT 4.2% intergenic (‑62/‑272) sthA/fabR pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB
?NC_000913 = 4499511 NA (NA)intergenic (+10/+83) insD1/yjgX IS2 transposase TnpB/pseudogene fragment