Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F17 I0 R1 115 121.7 2441348 96.4% 2353459 231.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,783,211 T→A 11.6% L150F (TTA→TTT)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,783,2110TA11.6% 237.1 / 2.3 87L150F (TTA→TTT) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base T (58/17);  new base A (2/9);  total (61/26)
Fisher's exact test for biased strand distribution p-value = 2.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCG  >  NC_000913/3782975‑3783446
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
aGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTaa                                                                                                                                                                                                                                            >  1:358954/1‑239 (MQ=255)
    gccaCATTCCAGCCAGCCCCCGGTGGACCAGCCCCGTACGCGCATCCGCACCAAAACCGATCCCGCCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCt                                                                                                                                                                                                                                        <  2:309010/236‑1 (MQ=255)
     tgcgctggcagccagcCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑CGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGCTCGGTCCAGGCCCCCGAAATAGCTGTCGATAAACCt                                                                                                                                                                                                                                        >  2:13603/9‑238 (MQ=255)
      gACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCAGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CAGCAGTGAAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGACGGACAACCTg                                                                                                                                                                                                                                       >  1:1125181/1‑238 (MQ=255)
       aCATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTgcc                                                                                                                                                                                                                                     >  1:1044459/1‑239 (MQ=255)
                  agccCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCtt                                                                                                                                                                                                                           >  1:1012159/1‑238 (MQ=255)
                    cccACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTc                                                                                                                                                                                                                         >  1:1189068/1‑238 (MQ=255)
                    cccACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTc                                                                                                                                                                                                                         >  1:121695/1‑238 (MQ=255)
                    cccACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTc                                                                                                                                                                                                                         >  1:829954/1‑238 (MQ=255)
                            tGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑Gt                                                                                                                                                                                                                <  1:307729/238‑1 (MQ=255)
                              aTCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTgg                                                                                                                                                                                                             >  1:116884/1‑239 (MQ=255)
                                     ccGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑AAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCagag                                                                                                                                                                                                      >  1:153038/1‑239 (MQ=255)
                                        tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGCGATCATCGG‑CGAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTCGGGCCAGAGATa                                                                                                                                                                                                   >  2:550828/1‑239 (MQ=255)
                                        tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGCTCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGGCAAC‑GTTGGGCCAGAGAtc                                                                                                                                                                                                   >  2:779792/1‑238 (MQ=255)
                                        tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCa                                                                                                                                                                                                                      >  2:82947/1‑221 (MQ=255)
                                        tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCa                                                                                                                                                                                                                      <  1:82947/221‑1 (MQ=255)
                                        tACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGGTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAAAGCGGAAAC‑TTTT‑G‑CCGCAGGGCAGCAGCTGCTGGAGTTCAGCGG‑CAAAGGTCTGAGCGGCCGAGGCCAGCGAAGGAGCTGTCGGTAAACCTGCCGCCAGTTC‑TTCCCAAACGGTTGGGcagcaggtc                                                                                                                                                                                                   >  2:823620/1‑231 (MQ=255)
                                          cgcgCATTCGCACCAACACCGATACCGTCAGACATCCCCGCACCAATGGCCATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAAACCGGCTTGCCGTAAACGCGGAACA‑TTTT‑G‑CCGCTGTGCAGCAGCTGTTGGCGATCATCGG‑CAATGGTCTGATCGGTCGTGGCCAGCCAGATAGCTGTCGTCACAACTGCCCCCAGTCTTTTGGCAAAC‑GTTGGGCGAGAGAtca                                                                                                                                                                                                  >  2:111366/1‑236 (MQ=255)
                                                  cGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCg                                                                                                                                                                                         >  1:603083/1‑239 (MQ=255)
                                                         aaaCCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCCATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAAGCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGCGATAACCGCCAGCGGGATTTg                                                                                                                                                                                  >  1:94154/1‑239 (MQ=255)
                                                          aaCCGACCCCGTCGGCAACCCCCGACCAAACGGCAATAAGTTTTTCCCCCGCG‑CCGCCAAGC‑TGACCGCCAATGAAACCCGGATCGCTGTAAACGCGG‑AAC‑TTTT‑GCCCGAAGTGCAGCGGCAGCTGGAGACACCCGG‑CAATGGTCTGATCGGTCGAGGCCAGCGAAATAGCGGTCGGTAAACCTGCCGCCAGTTCTTTCGAAACC‑GTGGGGCCAGAGATAACCGCCAGCGGAATTTGa                                                                                                                                                                                 <  2:967795/239‑1 (MQ=255)
                                                           cccgagccccccGGACACCCCCGC‑CCAATGGCAATGACCTTTTTCCCCCCGCCCCCCAGGG‑TGCGCGCAAAGGAACCCCGGGTGGCGGTAAACG‑GGAAAC‑TTTTGG‑CCGCGGTGCAGCGGCGGCTGGGGATAATCGG‑CAAAGGTCTGATCGGTCGAGGCAAGCGAAATAGCTGTGGGTAAACCGGCCGCAAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGAt                                                                                                                                                                                <  2:392679/228‑1 (MQ=255)
                                                               aTACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCg                                                                                                                                                                              >  1:209360/1‑237 (MQ=255)
                                                                acccgccgCACATCCCCGACCAAATGGCAATAACGTTTTACACCGCG‑CCGCAACGC‑TGCCCGCCAATGAAATCCGGATTGCGGTAACCGCGAAAAC‑TTTT‑G‑CCGCAGGGCAGCAGCTGCTGGATATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGGCGGTAACCCTGCCGCCAGTTCTTTCGCAAAC‑GTGGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCt                                                                                                                                                                           <  2:116884/237‑1 (MQ=255)
                                                                       ggACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGcc                                                                                                                                                                     >  1:1042627/1‑238 (MQ=255)
                                                                          cacccccGCAACATGGGAACTAATGGTTTCCACCGCG‑CCGCAAAGC‑TGCACGCCAATAAAATCCGGATGGCGGAAACCGCGAAACC‑TTTG‑G‑CCGGAGGGCGGCAGCTGGTGGAGTCCACCGG‑CAAGGGCCTAATCGGCCGGGGCCAGCGAAAGACCTGCGGGAAACCCGGCCGCAAGTTCTTTCGCAAGC‑GTTGGGCCAGCGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCAc                                                                                                                                                                 <  2:517045/236‑1 (MQ=255)
                                                                             ccccGCACCCATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGCCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GGTGGGCCAGAGATAACCGCCAGCGGAACTTGACAGTCTAAGCCCTCAcgc                                                                                                                                                               >  2:955996/1‑238 (MQ=255)
                                                                             ccccGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCAcgc                                                                                                                                                               >  1:1148173/1‑238 (MQ=255)
                                                                             ccccGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGC‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGACAGAGATAACCGCCAGCGGCATTTGATCGCCTAAGGCCTCAcgc                                                                                                                                                               >  2:246501/1‑238 (MQ=255)
                                                                              cccGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCAcgcg                                                                                                                                                              >  2:24897/1‑238 (MQ=255)
                                                                                ccccccAATGGAATTAGCGTTTTCCACCCCC‑CCGCAAAGC‑TGCACGCAAATGAAACCCGGAGTCGTTTAAACGCGAAAAT‑TTTT‑G‑CCGCAGGGCAGCGAGTGCTGGAGACCATCGC‑CAAAGGTCGGATGGGTCGAGCCCAGCAAAATAGCTGTCGGAAACCCTGCCGCCAGTTTTTTCGCAAAG‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCa                                                                                                                                                           <  2:189451/235‑1 (MQ=255)
                                                                                cccacaAATGACATTATCTTTTTTCCCCCCC‑CCACCACGC‑TGCACCCCAATGAAACCCGGTTTGCTGAAAACGCGAAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCGGGAGATCTGCGG‑CAAAGGTCTGTCCGGTCGGGCCCAGCGAAATGGCTGTCGTTAACCCTGCCGCCAGTTCTTCCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCa                                                                                                                                                           <  2:731588/237‑1 (MQ=255)
                                                                                  cACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGATATAACCGCCAGCGGAATTTGATCGCCTAACGCCTCACGCAcccc                                                                                                                                                          >  2:96678/1‑236 (MQ=255)
                                                                                       aTGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGCAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTCAGGCCTCACGCGCCACGt                                                                                                                                                        >  1:40176/1‑235 (MQ=255)
                                                                                       aTGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGt                                                                                                                                                        >  1:701287/1‑235 (MQ=255)
                                                                                       aTGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGt                                                                                                                                                        >  1:992022/1‑235 (MQ=255)
                                                                                       aTGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGt                                                                                                                                                        >  1:250280/1‑235 (MQ=255)
                                                                                       aTGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGt                                                                                                                                                        >  1:645918/1‑235 (MQ=255)
                                                                                             aTAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAg                                                                                                                                              >  1:304581/1‑239 (MQ=255)
                                                                                                             cg‑ccgcCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGACATAGCTGTCGGTAAACCTGCCGACAGTTCTTTCGCAACA‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACACCCTGTAACAGACGTCCGGTTTCCg                                                                                                                                >  2:848404/1‑237 (MQ=255)
                                                                                                                  gccAAGCTTCCACGCCAAGAAAAGCCGTATGGCTGTAACCG‑GGAACCTTTTT‑G‑CGGCGGCGGACCAGCGGCTGG‑GATCCACGGCCAAAGGCCAGACGGGTCGGGGCCGGCAAAATAGCTGTGGGAAACCCGGCCGCACTTTCTTTCGCAAAC‑TTGGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTc                                                                                                                            <  2:543344/238‑1 (MQ=255)
                                                                                                                  cccaagcggacaagaaaaTGAAATCCAGTTTGGTGTAAACGCGGAAAT‑TTTG‑G‑CCGCGGGGCCGCGGCTGCGGGAGATGATCGG‑TGAGGGTCGGACGGGCCGAGGCAAGCAAAATAGCTGGGGGAAACCCGGCCGCCAGTTCTTTCACAAAC‑GGGGGGCGAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTc                                                                                                                            <  2:226212/222‑1 (MQ=255)
                                                                                                                            cACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑Gt                                                                                                                                                                                                                <  1:553227/144‑1 (MQ=255)
                                                                                                                            cACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑Gt                                                                                                                                                                                                                >  2:553227/1‑144 (MQ=255)
                                                                                                                              cGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGt                                                                                                                >  1:370203/1‑238 (MQ=255)
                                                                                                                              cGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTc                                                                                                               >  1:932836/1‑239 (MQ=255)
                                                                                                                                    tGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCcac                                                                                                         >  1:962812/1‑239 (MQ=255)
                                                                                                                                                   tGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCa                                                                                          >  2:1129615/1‑239 (MQ=255)
                                                                                                                                                     tAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGCCGCGCATCAgg                                                                                        >  1:69892/1‑239 (MQ=255)
                                                                                                                                                              aacc‑TTTT‑G‑CGGCAGTGCGGCAGCGGCTGGAGACCAGCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTCCCGCCAGTTCTTCCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCa                                                                                <  1:71097/235‑1 (MQ=255)
                                                                                                                                                               aaC‑TTTT‑G‑CCACAGTGCAGCGGCGGCTGGAGACCATCGA‑CAAAGTGCTGACCGGTGGAGGCCAGCGAAATAGCTGTCGGTAACCCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATACCCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAg                                                                               <  1:982817/238‑1 (MQ=255)
                                                                                                                                                                     tt‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGCACGCATCAGTGGttt                                                                         >  1:731440/1‑239 (MQ=255)
                                                                                                                                                                        g‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTaa                                                                       >  1:442829/1‑239 (MQ=255)
                                                                                                                                                                                 gtgtaaaagagacaggaTCATCGG‑CAAGGGTCTGATGGCTCGGGGCCAGCGAAATGGCTGCCGGTAACCCTGCCGCCAGTTCTTTCGCAAAC‑GTGGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGgcg                                                                <  2:250570/223‑1 (MQ=255)
                                                                                                                                                                                 gcagcaGTTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAACC‑GTTGGGCAAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGgcg                                                                <  1:257281/238‑1 (MQ=255)
                                                                                                                                                                                          gctgGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTc                                                       >  1:189199/1‑238 (MQ=255)
                                                                                                                                                                                          gctgGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTc                                                       >  2:408825/1‑238 (MQ=255)
                                                                                                                                                                                          gctgGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTc                                                       >  2:258378/1‑238 (MQ=255)
                                                                                                                                                                                          gctgGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGCCGGAAAACATGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATTGCCTAAGGCCTCACGCGCCACGTACTGTAACAGAAGTCCGGTTTCCGCTTCCAGCCCTTTGGCCGCCCACACCAGACGCGCATCCGGAGCCATCAGGGGTTTAATCCGGCGCAGCACtac                                                       >  2:318528/1‑236 (MQ=255)
                                                                                                                                                                                             ggAGATGCTGGG‑CAAGGGCGTGATGGGACGACGCACGCAAAATACCTGCCGGAAACCCGGCCGCCAGTTCTTTCGCAACC‑GTTGGGCCAGAAATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCAcc                                                    <  2:304581/238‑1 (MQ=255)
                                                                                                                                                                                                   catcGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACa                                             >  1:118530/1‑239 (MQ=255)
                                                                                                                                                                                                   catcGG‑AAAAGGTCTGTCCGGTCGAGCCCGACGAAATGGCTGTCGGTAGTCCTGCCGCCAGTTCTTCCGCAACC‑GTGGGGCAGGAGATAGCCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACa                                             <  1:975882/239‑1 (MQ=255)
                                                                                                                                                                                                    ctcGG‑CAAAGGTGTGAGCGGGGACGGCCAGAAAAATAGCGGAAGGAAACCCCGCCGCCATTTCTTTCGCAAGC‑GTTGGGCGAGAGATAGCCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACa                                             <  1:388047/237‑1 (MQ=255)
                                                                                                                                                                                                     tcGG‑CAAAGGTCTGATCGGTCGAGGGCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATg                                           >  1:318835/1‑239 (MQ=255)
                                                                                                                                                                                                     tcGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATg                                           >  2:445800/1‑239 (MQ=255)
                                                                                                                                                                                                     tcGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCACCACTTCACCAAAgcaatg                                           >  2:746102/1‑234 (MQ=255)
                                                                                                                                                                                                       cggcacgtgtctgaccggtacgcgCCAGCGAAAGTGCTGTCAGACACCCCGCCGCCTGTTCTTCCGAAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTGCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATgg                                          <  2:684620/216‑1 (MQ=255)
                                                                                                                                                                                                            aaGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCa                                                      >  1:250570/1‑222 (MQ=255)
                                                                                                                                                                                                               gTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTac                                  >  2:817222/1‑239 (MQ=255)
                                                                                                                                                                                                                    aTCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTacggcg                              >  2:440469/1‑238 (MQ=255)
                                                                                                                                                                                                                    aTCGGTCGAGCCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCTCTTTGGGCGCCCACACCAGACGCGCATCAGGACGCGTCCGTGGTTTAATCTGGCCCAGCACTTCCCCACAGACATGCCCGGGTacgagg                              >  2:339432/1‑236 (MQ=255)
                                                                                                                                                                                                                     cggttccaGGCCAGCATAGTAGCTGCGTGAAAACCTCCCGCAATTGCCTCCGCAAGC‑GTTCGGCCAGCAATAACCGGCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTacgacgac                            <  2:543126/232‑1 (MQ=255)
                                                                                                                                                                                                                        gccggcgcacggaaaagcggggTCCGTAAGCCTGCCCGCAGTTCTTTCCAAACC‑GTTGGGCCAGAAAAAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTAcgacgacga                          <  2:779550/217‑1 (MQ=255)
                                                                                                                                                                                                                         tCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACgaga                        >  1:967384/1‑239 (MQ=255)
                                                                                                                                                                                                                         tCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACgaga                        >  1:519203/1‑239 (MQ=255)
                                                                                                                                                                                                                              gCCAGCGAAATAGCTGTCGTTAAACCTGCCGCCAGTTCTTTCGAAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGAt                                                                                                                                                                                <  1:840233/82‑1 (MQ=255)
                                                                                                                                                                                                                              gCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGAt                                                                                                                                                                                >  2:840233/1‑82 (MQ=255)
                                                                                                                                                                                                                              gCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGACCACACCAGACGCGCATCAGGACGCATCAGAGGTTTAATCTGGCGCAGCACTCCACCACAGACATGGCTGGGTACGACGACGAGAATAtt                   >  1:765770/1‑239 (MQ=255)
                                                                                                                                                                                                                                cAGCGAAATAGCTGTCGGTAACCCTGCCGCAATTTCTTTCGCAAAC‑GTGGGGCAAGATATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTAc                 <  1:327306/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCTCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACg                >  1:869195/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGCCGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCCACGACATGGCTGGGCACGCCGCCGAGATTCTTACg                >  2:375630/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACg                >  2:812857/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACg                >  2:1135653/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACg                >  1:223729/1‑237 (MQ=255)
                                                                                                                                                                                                                                     aaagacgtGTCGGAAACCCCGCCGCCAGTTCTTTCGCAACC‑GGTGGGCCGGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACggctg            <  2:992022/232‑1 (MQ=255)
                                                                                                                                                                                                                                            tGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCa     >  1:545596/1‑239 (MQ=255)
                                                                                                                                                                                                                                               cGGTAACCCGGCCGCCAGTTTGTCGGCAACC‑GGTGGGCCAGCTACAACCGCCAGCGGAATTTGATCGCCTAAGGCTTCACGCGCCACGTCCTGTAACAGGCGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGTCGACGAGAATATTACGGCTGGCTGCCAgcg  <  2:121695/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
AGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCG‑CCGCCAAGC‑TGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAAC‑TTTT‑G‑CCGCAGTGCAGCAGCTGCTGGAGATCATCGG‑CAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAAC‑GTTGGGCCAGAGATAACCGCCAGCGGAATTTGATCGCCTAAGGCCTCACGCGCCACGTCCTGTAACAGACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCG  >  NC_000913/3782975‑3783446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A