Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F17 I0 R1 115 121.7 2441348 96.4% 2353459 231.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,395,305 T→G 13.3% G413G (GGT→GGG)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,3050TG13.3% 312.1 / 5.0 130G413G (GGT→GGG) nnrbifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base T (44/61);  new base G (18/2);  total (67/63)
Fisher's exact test for biased strand distribution p-value = 5.99e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

GGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCC  >  NC_000913/4395072‑4395542
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               
gggAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCgg                                                                                                                                                                                                                                            >  1:972972/1‑239 (MQ=255)
gggAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCgg                                                                                                                                                                                                                                            >  1:670658/1‑239 (MQ=255)
gggAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCg                                                                                                                                                                                                                                             <  1:1196316/238‑1 (MQ=255)
            cTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCAGATGCATTGAACCTGCTGGCAATCAATCAAGATAAGCGTCACAATCGCGTGATTACGCCGCATCCGGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTGGCTGTAGCTAAACGTGACCGCCTAGTTTGCGCCAAACGTCTGGACCAACGTAATGGTGGCGGACCCGTGCTCGCAGGAACCGGCACCGAGGTca                                                                                                                                                                                                                                 >  2:255605/1‑237 (MQ=255)
             tGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTcgc                                                                                                                                                                                                                                >  1:801409/1‑238 (MQ=255)
                       tGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCt                                                                                                                                                                                                                      <  1:593853/238‑1 (MQ=255)
                       tGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAAGTGCCGGGACCGTGGGCGCCGCCCATCCTg                                                                                                                                                                                                                     >  1:369170/1‑239 (MQ=255)
                       tGAGAATGTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTg                                                                                                                                                                                                                     <  1:870249/239‑1 (MQ=255)
                        gtgaATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGa                                                                                                                                                                                                                    <  2:1133852/237‑1 (MQ=255)
                        gagaATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGa                                                                                                                                                                                                                    <  1:717757/239‑1 (MQ=255)
                        gagaATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGa                                                                                                                                                                                                                    <  1:132661/239‑1 (MQ=255)
                         agaATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGa                                                                                                                                                                                                                    <  2:684076/238‑1 (MQ=255)
                              tttCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCttt                                                                                                                                                                                                              <  2:801409/239‑1 (MQ=255)
                              tttCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCttt                                                                                                                                                                                                              <  1:227181/239‑1 (MQ=255)
                              tttCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAAGCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCttt                                                                                                                                                                                                              <  1:522918/239‑1 (MQ=255)
                                         gATGTTGTGGGAAGCCTAGGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTg                                                                                                                                                                                                   <  1:386684/239‑1 (MQ=255)
                                                tgGGAGGCAGATGCATTGTACCTCCTGGCAATCAATCCCGTTAAGCGTCACAATCGCGTGATCACGCCGCATCTTGGCGAGGCCCCACGGTTGTTACGCTGTCCCGCCGCTGAAATTGAAAGTGCCCCCTTACATTTCGCCAAACGACTGGTACACTGTTATTGCGGCGCATCGGCTCTGTACGGCGCCCGGACCAGGGTTGCCGCCGGTCCGGACGCCTTAGGAGTTATTGCGGC‑Cgg                                                                                                                                                                                           >  2:575403/1‑239 (MQ=255)
                                                     tGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAACCGTCTGGTACAACGTTATGGCGGCGTAGCGGGGCTGAAAGGGGACGGCACCGGGGTCCCCGCCCACTCTGACGCTGTCGGCATTATTGCTgtcccgtat                                                                                                                                                                                        >  2:542269/1‑230 (MQ=255)
                                                     tGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCGCCCCTCCTGACGCCTTAGGCATTATTTGTGC‑CGGAAAt                                                                                                                                                                                       >  2:387451/1‑238 (MQ=255)
                                                     tGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGGGCTTACAGGTGCCGGGACCGGGGTAGCCGCCCCTCCTGACGCCTTCGGCGTTATTGATGCGCGGaatc                                                                                                                                                                                       >  2:747363/1‑237 (MQ=255)
                                                     tGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGAACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGTGCCAAACGTCTGGTACAACGTTATGGCGGAGTATCGGTGATGAAAAGGGCCGGCACCCTGTGCGCCGCCCACCCTTACCCTTTTGGGATGTTTGTTGC‑Cggcgag                                                                                                                                                                                       >  2:831899/1‑234 (MQ=255)
                                                       ccGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGGTGAAAGGTGCCGGAACCGTGGTAGCCCCCCATCCTGACGCTTTAGGCGTTATGGATGC‑CGGGAATgaa                                                                                                                                                                                    >  2:789722/1‑237 (MQ=255)
                                                       ccGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGGGCTGAAAGGGGCCGGGACCGCGGGCGCCGCCCCACCTGGCGCTTTAGGCATGATTGGGTC‑CGGaaagggc                                                                                                                                                                                    >  2:240215/1‑235 (MQ=255)
                                                             cATTGAACCTGCGGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGAGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCt                                                                                                                                                                                                                <  1:86875/206‑1 (MQ=255)
                                                                     ctgctgGCAATCAAGCCCGATAAGCGTCACAATCGCGTGATCACGCCGCAGCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAgcgg                                                                                                                                                                      <  1:1090418/239‑1 (MQ=255)
                                                                       gctgGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAACGGTGCCGGAACCGGGGTCGCCACCCATCCTTAAGCTTTAGGCCTTATTGATGC‑CGGAAATGCAGGCATGGCGCgcgggg                                                                                                                                                                    >  2:498427/1‑237 (MQ=255)
                                                                       gctgGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAgcggcg                                                                                                                                                                    >  1:142828/1‑239 (MQ=255)
                                                                       gctgGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGGAACGGGGCTGAAAGGAGCCGGAAACGGGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAACAGCAGGCCTGGCGAgcggcg                                                                                                                                                                    >  2:701501/1‑239 (MQ=255)
                                                                        ctgGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGAGCCGGAACCGTGGTCGCCGCCCATCCTGTCGCTTTAGGCATTAATGATGC‑CGGAATTGCAGGCATGGCGCacggcg                                                                                                                                                                    >  2:707053/1‑238 (MQ=255)
                                                                              atcaatcCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGc                                                                                                                                                                                                          <  1:1003778/195‑1 (MQ=255)
                                                                                caatcCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCg                                                                                                                                                           <  2:1101377/239‑1 (MQ=255)
                                                                                     ccGATAAGTGTCACAGTCCCGTGATCACGCCGCATCCTGGCGAGGCCGAACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                                                                                                                      <  2:407042/239‑1 (MQ=255)
                                                                                     ccGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                                                                                                                      <  1:180178/239‑1 (MQ=255)
                                                                                     ccGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                                                                                                                      <  1:103919/239‑1 (MQ=255)
                                                                                     ccGATAAGCGTCACAAGCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCGTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                                                                                                                      <  1:780987/239‑1 (MQ=255)
                                                                                      cGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCGCCCATCCTGACGCGTTAGGCATTTTTGCAGC‑CGGAAATGCAGGCCTGGGCGGGGGCGGCAGGGGCGAtgt                                                                                                                                                       >  2:270322/1‑237 (MQ=255)
                                                                                       gATAAGCGTCACGAGTGCGTGATCACGCAGCATCCTGGCGAGGCCGCACGGTTGTTATGCTGTTCCTTCGCTGAAAGTGAAAGTGACCGCGTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGct                                                                                                                                                    <  2:1165422/239‑1 (MQ=255)
                                                                                       gATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGct                                                                                                                                                    <  1:38003/239‑1 (MQ=255)
                                                                                         tAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctct                                                                                                                                                  <  1:831899/239‑1 (MQ=255)
                                                                                             cGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGACAGGTGCCGGAACCGTGGCCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCCTGGCGAGCGGCGGGCCGGGCGCTGTGCTCTTTgg                                                                                                                                              >  2:959818/1‑239 (MQ=255)
                                                                                                 acaATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCCCCGCCCATCCTGACGCTTTAGGCATTATTGGTGC‑CGGAAATGCAGGCATGGCGGACGGCGGCATGGGCGATGTGCTCTCTGGtttt                                                                                                                                          >  1:1211704/1‑239 (MQ=255)
                                                                                                       gcgTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGgc                                                                                                                                    >  1:71847/1‑239 (MQ=255)
                                                                                                          tGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGTTGTGCTCTCTGGTATTATTGGCGCa                                                                                                                                 >  1:1012650/1‑239 (MQ=255)
                                                                                                                     cATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCa                                                                                                                      >  1:210620/1‑239 (MQ=255)
                                                                                                                              gAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGt                                                                                                              <  1:656718/238‑1 (MQ=255)
                                                                                                                              gAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGt                                                                                                              <  2:285899/238‑1 (MQ=255)
                                                                                                                                tgCCGCACGGTTGCTATGCTGTTCCGTCTCTGAAATTGAAAGTGACCGCGTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                                                                                            <  2:377136/237‑1 (MQ=255)
                                                                                                                                ggCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                                                                                            <  2:1148391/238‑1 (MQ=255)
                                                                                                                                ggCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                                                                                            <  1:684875/238‑1 (MQ=255)
                                                                                                                                ggCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGACGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                                                                                            <  2:985127/238‑1 (MQ=255)
                                                                                                                                 gCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTg                                                                                                                                                                                                                     >  2:986220/1‑133 (MQ=255)
                                                                                                                                 gCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGGATTATTGGCGCATTGCTTGGGCAAAAACTGTCGcc                                                                                                          >  1:757497/1‑239 (MQ=255)
                                                                                                                                   cGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTTGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGt                                                                                                        <  2:726732/239‑1 (MQ=255)
                                                                                                                                   cGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGt                                                                                                        <  2:1012650/239‑1 (MQ=255)
                                                                                                                                   cGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGt                                                                                                        <  1:105923/239‑1 (MQ=255)
                                                                                                                                        ggTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatgat                                                                                                   <  2:226459/239‑1 (MQ=255)
                                                                                                                                            gttAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                                                                                               <  2:786564/239‑1 (MQ=255)
                                                                                                                                            gttAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                                                                                               <  1:575403/239‑1 (MQ=255)
                                                                                                                                                  cTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgt                                                                                          <  1:747363/238‑1 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGGAAGGTGCCGGAACCGTGGGCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGGGCTCTCTGGGATTATTGGCGCATTGCTTGGGCAAAAACTCGCGCCGTGTTATGCGGACtgtgga                                                                                       >  2:664280/1‑237 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGGGGCATGGGCGATGTGCTCTCTGGTGTTATTGGCGCCTTGCTTGGGCAAAAACTGTCGCCGTATGATGAGGCCTGGGCa                                                                                       >  2:842538/1‑239 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctct                                                                                                                                                  >  1:884681/1‑180 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctct                                                                                                                                                  <  2:884681/180‑1 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCCCCCATCCTGAAGCTTTAAGCATTATTGATGC‑CGGAAATGCAGGCATTGCTCGCCGCGGCATGGGCCGTGGGGTCTCTGGCATTTATGGGCCCTTGCTTGGGCCAAAAATGTCGCCggactctgccggctttgcc                                                                                       >  2:807216/1‑221 (MQ=255)
                                                                                                                                                    gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGGCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCCTGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAACAACTCTCGCCGGATGATACAGCCTGTGCa                                                                                       >  1:272926/1‑239 (MQ=255)
                                                                                                                                                     ttCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGGCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGGGGCAGGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg                                                                                      >  1:606297/1‑239 (MQ=255)
                                                                                                                                                      tCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAgg                                                                                     <  1:959818/239‑1 (MQ=255)
                                                                                                                                                      tCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAgg                                                                                     <  1:347993/239‑1 (MQ=255)
                                                                                                                                                      tCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAgg                                                                                     <  2:658662/239‑1 (MQ=255)
                                                                                                                                                      tCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGCACAgg                                                                                     <  2:834554/239‑1 (MQ=255)
                                                                                                                                                          tCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGGGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt                                                                                 >  1:1074818/1‑239 (MQ=255)
                                                                                                                                                           cGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgtg                                                                                >  1:551847/1‑239 (MQ=255)
                                                                                                                                                               gAAATTGAAAGTGACCGCTTACATTGCGCCCAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGGAAGGTGCCCGAGCCGTGGTCTCCCCCCATCCTGACGCTTTCGGCATTATTGATGC‑CGGGAATGCCGGCCTGGCGGGCGGCGGCAAGGGCGATCTGCTCTCTGGTATTTTTTGTGCCTTGGTTGGGCACAACCTTCTGCCGGATGGTGCacaccgttaggactgtgacgc                                                                            >  2:902897/1‑219 (MQ=255)
                                                                                                                                                                     gAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACg                                                                       <  1:3658/238‑1 (MQ=255)
                                                                                                                                                                           gACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGc                                                                <  1:250502/239‑1 (MQ=255)
                                                                                                                                                                            aCCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTTCTGAAAGGGGCCGGAAACGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGA‑CGGAAATGCAGGCCTGGCGAGCGGCGGCACGGGTGATGTGCTCTCTGGGATTATTGGCGCATTGCTGGGGCAAAAACTGTCGCGGTATGATGCAGACTGTGCCGGCAGTGTCGGTGAAGGTGCggga                                                               >  2:299744/1‑237 (MQ=255)
                                                                                                                                                                            aCCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCa                                                               >  1:1099966/1‑239 (MQ=255)
                                                                                                                                                                                         cgcCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGCGGCCGGAACCGAGGTCGCCGCCCATCCTCACGCTTTAAGCATTATTGATGC‑CGGAAATGCCGGCATGGCGAGCGGCGGCCTGGGCGCTGTGCTCCCTGGCATTATTGGCGCATTGCTTGGGCAAACACTGTCGCCGTATGATGCAGCCTGTGCAGGCCGTGCCGCGCACGGTGCGCCGCCTGACGTACTg                                                   >  2:133345/1‑238 (MQ=255)
                                                                                                                                                                                              aaCGTCTGGTACAACGTTATGGCGGCGTAGCTGTGTTGAAAGGGGCCGGCACCGGGGTCGCCGCCCACCCTGACGCCTTAGGCATTATTGATGC‑CGGAAATGCAGGCACGGCGAGCGGCGGCATGGGCGGTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGGAAAAACTGTCTCCGTGTTATGCAGTCTGTGCACGCTTTGGCGCGACAGGTGCGGCCGGTCGCCTCCTggcggc                                              >  2:417462/1‑238 (MQ=255)
                                                                                                                                                                                                       tACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGcc                                                                                             <  1:790681/182‑1 (MQ=255)
                                                                                                                                                                                                           aCGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgcg                                                                           <  2:889288/196‑1 (MQ=255)
                                                                                                                                                                                                           aCGTTATGGCGGCGTAGCGGGGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgcg                                                                           >  1:889288/1‑196 (MQ=255)
                                                                                                                                                                                                            cGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCGCCCATCCTGCCGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCCTGGCGAGCGGCGGCCTGGGCGGTGTGCTCTCTGGGTTTATTGGCGCATTGGTTGGGCAAAAACTGTCGCCGTCTGATTGAGCCTTGTCAGGCTGTGTCGCGCACGGAGGCGCAGCTGACGTACTTGCGGcg‑cgggtttgagagc                               >  2:742139/1‑227 (MQ=255)
                                                                                                                                                                                                             gTTAGGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGAGGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAAcgcgc                             <  2:114982/239‑1 (MQ=255)
                                                                                                                                                                                                              ttATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGGGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAAcgcgc                             <  2:972972/238‑1 (MQ=255)
                                                                                                                                                                                                                aTGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCggg                          <  1:725654/239‑1 (MQ=255)
                                                                                                                                                                                                                aTGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTTTGCAGGGTGTGTTGCGCACGGGGCGGCGGCTGACGTACTGGCGGCG‑CGGTTTTTGGCGCGCggg                          >  2:677241/1‑239 (MQ=255)
                                                                                                                                                                                                                 tGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGCATGATGCAACCTGTGCCGGCTGTCTCGCGCACGGTGCGGCAGCTGACGTACCTGCGGCG‑CGGTTTGGAAccgccgggg                         >  2:954746/1‑231 (MQ=255)
                                                                                                                                                                                                                 tGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGGCGCCGGATGATGCAACCTGGGCAGGCt                                                                                   >  2:790681/1‑182 (MQ=255)
                                                                                                                                                                                                                 tGGCGGCGAAGCGGTGCTGAAAGGCGCCGGAAACGTGGTCGCCGCCCCTCCTGACGCTTTAGGCATTATTGGTGC‑CGGAAATGCAGGCATGGCGGGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGGAAAAACAGTCGCCGTATGATGCTGCCTTTGCAGGCTGTGTCGCGCCCGGTGCGGCAGCTGACGTACGCGGGGGG‑CGTTTTGGTAccgagcgga                         >  2:1083008/1‑231 (MQ=255)
                                                                                                                                                                                                                      gcgTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCCTGGCGGGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCCTTGCTTGGGCAAAAACTGTTGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGGGCGGCAGCTGACGCACGGGGGGCG‑AGCTTTGTCACGCGCgggggggtg                    >  2:378231/1‑233 (MQ=255)
                                                                                                                                                                                                                      gcgTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGCTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCCAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGCCCGCTCACGGTCTGGGGGCG‑CGTTTTTGAGCGCGCGGGGTgggg                    >  2:940966/1‑236 (MQ=255)
                                                                                                                                                                                                                      gcgTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCGGGATGCTg                    >  1:2462/1‑239 (MQ=255)
                                                                                                                                                                                                                       cgTAGCGGTGCTGAAAGGTGCCGGAACCGGGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGGGATGTGCTCCCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCCGCCTGTGCAGGCTGTTTCGCGCACGGTGCGGCCGCTGACGGACTGGCGGGGCCGtttttatggcgccgggggctgg                    >  2:772824/1‑221 (MQ=255)
                                                                                                                                                                                                                       cgTAGCCGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCCTGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAACCTGTGCAGGCTGTGTCGCGCACGGGGGGGCAGCTGACGTACTGGGGGGG‑GGGTTTGGGAAGCGCGGGGTgtggg                   >  2:11698/1‑235 (MQ=255)
                                                                                                                                                                                                                         tAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGGACTGGCGGCG‑CGTTTTGGAACGCGCGGGGGGCTGGCa                 >  1:633958/1‑239 (MQ=255)
                                                                                                                                                                                                                                ggtGAAAGGGTGCGGAACCGTGGTCGGCGGCCATCCTGGCGCTTTAGGCATTATTGATGG‑CGGAAATGCAGGCATGGCGTGCGGCGGCATGGGCGATGTGCTCGCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGGCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAGCGCGCGGGATGCTGGCAACCGAt           <  2:391034/236‑1 (MQ=255)
                                                                                                                                                                                                                                 cTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCGGGGTGCTGGCAACCGAtc          >  1:30030/1‑238 (MQ=255)
                                                                                                                                                                                                                                         tGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTcc  >  1:27478/1‑238 (MQ=255)
                                                                                                                                                                                                                                         tGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGCCGGCG‑CGGTTTGGAACGCGCGGGGTGCCGGAAACCGATCTCTTTTcc  >  1:633535/1‑238 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               
GGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGC‑CGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCG‑CGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCC  >  NC_000913/4395072‑4395542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A