Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F9 I0 R1	103	124.7	2465218	97.5%	2403587	230.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,478,418	G→T	12.9%	A65S (GCG→TCG)	ynbB →	putative CDP‑diglyceride synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,418	0	G	T	12.9%	296.6 / 6.8	94	A65S (GCG→TCG)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand): ref base G (26/55); new base T (11/2); total (37/57)
Fisher's exact test for biased strand distribution p-value = 4.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTGGGG  >  NC_000913/1478194‑1478647

cGGAGTGTGCTTCTTATGAGCTCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtttt                                                                                                                                                                                                                          <  2:1054135/239‑1 (MQ=255)

       tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTATGGGGGG‑GAAAAGGGGG‑GGGGGTATAATACGTATTTGTATCTGGTGGGGGATCGTGCTTTGTTTTTTTTTTGTTATTTTAGGTGCACGCTGGGTGACGTTTACGCTTTTTTTGTTGTTTAGGTTTCT‑gtcgt                                                                                                                                                                                                                  >  1:202443/1‑235 (MQ=255)

       tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  2:1210420/239‑1 (MQ=255)

       tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTACCGGGGC‑GGAAAAGGGGGGAGGGTACATTACGTATTCGTTACTGGTGGGGCGTCGTCATTTGTTTTTTATTGGTGTTTTTAGGTGCATCCTGGCTTAGGCTATGGTTTTTTGCGCTGGTAACttttttcgtct                                                                                                                                                                                                                   >  1:995483/1‑232 (MQ=255)

        tcttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  1:1099413/237‑1 (MQ=255)

        gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCAt                                                                                                                                                                                                                  <  2:109902/238‑1 (MQ=255)

          ttctgATGAGCGCTGAAAGAGAAATCTGGAAAAATCTCTGGCATCACTTTTCGCGTTGTTAAGTTTAGCAACGCTGATAAACCGTTTTCTGCGATGGCGG‑TAACCGGAGA‑GAAAAGGGGGGAAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGGCATTTGTTTTTCAATGGTGAATTCAGGTCCACGCTGGATGACGTTGACGTTATTTGCGCTGATAAGTTTGCT‑GGCATTgt                                                                                                                                                                                                               <  1:328174/239‑2 (MQ=255)

           tcttATGATCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACCCTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGa                                                                                                                                                                                                               <  1:417609/238‑1 (MQ=255)

             ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGG‑GAAAAGGGGGGGAGGTTACATTTCGTATTCGTACCTAGTGGGGGATCGGCATTTGTTTTTTTTTTGTGATTTTATGTCCACGGTGGCTGGCGCTTAGGTTTTTTTTGCTTGTATGTTTTTT‑TGCATTGAAAg                                                                                                                                                                                                            >  1:38646/1‑239 (MQ=255)

              tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTACTGGGGAGGAAAAGGGGGGGGGGGTTCATTTATTGTT‑GTATCTTGTTGGGCAACGGCTTTTGTTTTTTTATGGGGTTTTTCGGACCCCGCTGGGTGGCGGTTTCGGTTTTTTTGCTGATGTGGTTTTT‑TGCATTGtaaaa                                                                                                                                                                                                           >  1:573757/1‑237 (MQ=255)

                 gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAata                                                                                                                                                                                                        <  2:346535/239‑1 (MQ=255)

                    cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGA‑GAAAAGGGGGGGGGGGTTAATTATGTATTCGGTACTGGGGGGGGCTCGTCATTTGTTTTTTAATTGGTATTTTTGGTGCCCGCTGGGCGATGTTGAGGTTTTTTTTGCGTGTAAGTTTTTT‑GGCATTGTAAAGATAttt                                                                                                                                                                                                     >  1:731212/1‑238 (MQ=255)

                     gcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTGTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTg                                                                                                                                                                                                     <  2:409793/238‑1 (MQ=255)

                          aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCTATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGt                                                                                                                                                                                                    <  2:26064/234‑1 (MQ=255)

                                aTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATc                                                                                                                                                                                         <  2:816/239‑1 (MQ=255)

                                 tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCAGGGG‑GAAAAGGGGGGGGGGGTTCATTAAGTATTCGTCTCTTGTGGGGGGTCGTCATTTTTTTTTTAATGGTGTTTTTTTGTTCACGCTGGGTGAGGTTTACGTTTTTTTTGCTGATTAGGTTTTT‑TGCCTTGATAGAATATTTTTCGCtttttttt                                                                                                                                                                                        >  1:998259/1‑233 (MQ=255)

                                 tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGA‑GAAAAGGGGGGGTGGGTACATTACGAATTCGTACCTTGTGGGGCGACGTCATTTGTTTTTTATAGGTGATTTGAGTTCAACGCTGGGGTACGGTTTCGTTTTTTTTGTTGCTAACTTTTCT‑TGCCTTGTAGGAATAttttttcgtttttttt                                                                                                                                                                                        >  1:923687/1‑225 (MQ=255)

                                    tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCTCCCGG‑GAAAAAGGGGGGAGGCTACATTTCGTTCCCTGTCCTGCTGGGGCATCCCCACTTTTTGTTCTCTTGGGCTTTTAGCTCGACCCTCGGCCACCTTGACGTTTTTTTCGGCGATAACTTTTCT‑TTCGTTGAAAGAATAtttgttgctttttttcttg                                                                                                                                                                                     >  1:26064/1‑222 (MQ=255)

                                              tctGGCAACACTTTTCGAGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGGCATTTGTTTTGCAATGGTGATTTCAGTTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGGATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgg                                                                                                                                                                           <  2:151055/239‑2 (MQ=255)

                                              tctGGCAACACGTCTCGCGTTGTTAATTTTAGCAACGGTGATAAACCGTTTTCTGGTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGAGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTCTTAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgc                                                                                                                                                                           <  1:484365/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACTTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:661123/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:301005/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:1083403/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:301500/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:387149/239‑1 (MQ=255)

                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  1:752229/239‑1 (MQ=255)

                                               ctGGCAACACGTTTCGCGTTGTTAAGTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTCGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  2:799656/239‑1 (MQ=255)

                                               ctGGCAACAATTTTCGCGGTGTTTATTTTATCAACGCTGATAAACCGTGTTCGTGTATTGCGG‑TTTCCCGATA‑GAAAAAGGGGTGAGGTTACATTGCGTATTCGTACCTGGTGCGTCATCGTCATGTGTGTTTCAATGGTAATTTCAGTTCCACGATGGATGACGTTCGCGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTGCcgcg                                                                                                                                                                          <  1:497225/239‑1 (MQ=255)

                                                tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCcgcg                                                                                                                                                                          <  1:1007019/238‑1 (MQ=255)

                                                tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGt                                                                                                                                                                         <  2:779471/239‑1 (MQ=255)

                                                        cTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTtata                                                                                                                                                                 <  1:1032872/239‑1 (MQ=255)

                                                          tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGG‑GAAAAGGGGGGGAGGGTTAATTACGTTTTTGTACCTGGTGGGGGAGCGTCATTTGTTTTTTTATGGTGGTTTTTTGTCCCCGCTGGGTGGAGTTGTCGTTTTTTTTGCTGTTAAGTTTTTT‑GGGCTTTTAAAAATATTGTTAGCTTTTTTTTGTTCATTTTTTGCGCTGGttttttt                                                                                                                                                               >  1:898805/1‑234 (MQ=255)

                                                           ttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTg                                                                                                                                                              <  1:1205835/239‑1 (MQ=255)

                                                                gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa                                                                                                                                                         <  2:573096/239‑1 (MQ=255)

                                                                  tgttAATTTTAGCAACGGTTATAAACCGGTTTTTTCTATGGGTG‑TTACCGGACA‑GACACGGGGGTGTGGTTAGCTTAGTTATTCCTTCCTTGTGGGTCTTGGTCGTTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTAGCGTTTTTTTCGCTGATAATTTTTCT‑GGCATTGAAATAATATTGTACTCTTATATCTGTACATTTTCCGCTTTGGTTATATTGGGGTAtt                                                                                                                                                       <  1:25546/239‑1 (MQ=255)

                                                                  tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTAtt                                                                                                                                                       <  2:373739/239‑1 (MQ=255)

                                                                        ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGTTGAGGTTTCATTACGTATTCGTACCTGGTGTGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCtt                                                                                                                                                 <  2:779193/239‑1 (MQ=255)

                                                                        ttgtAGGAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAGCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATTGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTTTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCtt                                                                                                                                                 <  1:153451/236‑1 (MQ=255)

                                                                         tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTTCGTATTCGTACCTGGGGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTGTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTa                                                                                                                                                <  2:614883/239‑1 (MQ=255)

                                                                            aGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GGAAAGGGGGGGGGGGTACATTACGTATTTGTACCCGGTGGGGCCTCGTCATTTTTTTTTTTATGGTGGTTTTAGGGCCCCGCTGGATGGCGTTGACGGTTTTTTTGCTGATAAGTTTTTTGGGCTTTGAAAAATAATTTTACG‑TTTTATTTGTACCTTTTTCGCGTTTGTTATTTTTGGGTATTTCTCTTTACt                                                                                                                                             >  2:931624/1‑239 (MQ=255)

                                                                            aGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAAGGGGGGGGGGTTCATTACGTATTCGTACCTGGGGGGGCATCGTCCTTTTTTTTTTTATGGGGGTTTTTGGTCCACGCTGGGTGACGTTTTCGTTTTTTTTGCTGGTAAGGTTTTT‑TGCATTTGAAGAATATTTTACGCTTTTATCTTTACATTTTTCGCGTTGGTTATATTTGGGGTTTCCTCTTAACt                                                                                                                                             >  2:259193/1‑239 (MQ=255)

                                                                                 cGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGAGA‑GAAAAAGGGGGGAGGGTAATTTTGGTGTTTTTTCCGCGGGGGGGAACGTCCTTTGTTTTTCACAGGTGATTTTAGTGCCAAGCTGTGTGAAGTTTAAGGTTTTTGCGCTGATATGTTTTTT‑TGTATTGTTAGGATATTGTAAGCTTTTATCTGTGCACTTTCCGCGTTGGGGATATTTTGGTAttttccttgatcttttt                                                                                                                                        >  1:637113/1‑224 (MQ=255)

                                                                                              ttttCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGGGGGGGTAAATTACGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATGGGGGTTTTAGGGCCACGCTGGATGACGTTGTCGTTTTTTTCGCTGGTAAGTTTTTT‑GGGATTTTAAGAATATTTTACGCTTATATTTGTACATTTTTTGCGTTGGGTATATTGGGGTATTTTTCTTTACTATTTTCTTATTGGttttt                                                                                                                           >  2:322248/1‑238 (MQ=255)

                                                                                               tttCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTaa                                                                                                                          <  2:546735/239‑1 (MQ=255)

                                                                                                    gCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCt                                                                                                                     <  1:171712/239‑1 (MQ=255)

                                                                                                    gCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCt                                                                                                                     <  1:1112833/239‑1 (MQ=255)

                                                                                                           cGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTTCACGCTGGATGACGTTGACGTTTTTTTCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc                                                                                                               <  2:637113/238‑1 (MQ=255)

                                                                                                                   cGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTg                                                                                                       <  1:420874/239‑1 (MQ=255)

                                                                                                                   cGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTg                                                                                                       <  2:998259/239‑1 (MQ=255)

                                                                                                                                ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa                                                                                                                                    >  1:1106750/1‑198 (MQ=255)

                                                                                                                                ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa                                                                                                                                    <  2:1106750/198‑1 (MQ=255)

                                                                                                                                     gatgTTACATTACGTATTCTTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGc                                                                                      <  1:852616/236‑1 (MQ=255)

                                                                                                                                     gAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTg                                                                                        >  1:340178/1‑237 (MQ=255)

                                                                                                                                     gAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTg                                                                                        >  1:441735/1‑237 (MQ=255)

                                                                                                                                         ttACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTg                                                                                    <  1:55546/237‑1 (MQ=255)

                                                                                                                                          tACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGt                                                                                                            >  2:166552/1‑212 (MQ=255)

                                                                                                                                          tACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGt                                                                                                            <  1:166552/212‑1 (MQ=255)

                                                                                                                                             aTTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTc                                                                              <  2:202443/239‑1 (MQ=255)

                                                                                                                                             aTTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTc                                                                              <  2:38646/239‑1 (MQ=255)

                                                                                                                                                 cGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGat                                                                          >  1:942658/1‑239 (MQ=255)

                                                                                                                                                       cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCGGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGaaa                                                                                                                                                                                                             >  1:434584/1‑102 (MQ=255)

                                                                                                                                                       cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCGGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGaaa                                                                                                                                                                                                             <  2:434584/102‑1 (MQ=255)

                                                                                                                                                       cGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGc                                                                    <  2:244585/239‑1 (MQ=255)

                                                                                                                                                          aCCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCa                                                                  <  1:1191673/238‑1 (MQ=255)

                                                                                                                                                           ccTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCAcc                                                                >  2:824398/1‑239 (MQ=255)

                                                                                                                                                             tggtggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCgg                                                              >  2:1014059/1‑239 (MQ=255)

                                                                                                                                                              ggtggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCgg                                                              >  2:186255/1‑238 (MQ=255)

                                                                                                                                                                tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCa                                                           >  2:299102/1‑239 (MQ=255)

                                                                                                                                                                tggGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATAGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGTCa                                                           >  2:1198410/1‑239 (MQ=255)

                                                                                                                                                                  gggCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCa                                                                                                                                                                                                                   <  2:539304/85‑1 (MQ=255)

                                                                                                                                                                  gggCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGCTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTATTTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAg                                                         >  1:905908/1‑239 (MQ=255)

                                                                                                                                                                  gggCATCGTCATTTGTATTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCa                                                                                                                                                                                                                   >  1:539304/1‑85 (MQ=255)

                                                                                                                                                                    gCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGCTATTAGCCACCGGGCAAGtt                                                       >  1:555142/1‑239 (MQ=255)

                                                                                                                                                                       tCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTa                                                    <  2:731212/239‑1 (MQ=255)

                                                                                                                                                                        cGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAg                                                   <  2:544578/239‑1 (MQ=255)

                                                                                                                                                                         gtgaTTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGATATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGt                                                  <  1:54124/236‑1 (MQ=255)

                                                                                                                                                                               tGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTg                                             >  2:920745/1‑238 (MQ=255)

                                                                                                                                                                                  ttttCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGAccc                                         <  2:441735/239‑1 (MQ=255)

                                                                                                                                                                                     tCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTTTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  2:923687/239‑1 (MQ=255)

                                                                                                                                                                                     tCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  1:322248/239‑1 (MQ=255)

                                                                                                                                                                                         tGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGt                                  <  2:299347/239‑1 (MQ=255)

                                                                                                                                                                                          ggTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGtt                                 <  2:289348/239‑1 (MQ=255)

                                                                                                                                                                                           gTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGttt                                >  2:97042/1‑239 (MQ=255)

                                                                                                                                                                                              aTTTCAGTTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGTGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGTTGACCCCTCTGGttt                                >  1:1158923/1‑236 (MQ=255)

                                                                                                                                                                                              aTTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGttt                                >  2:608078/1‑236 (MQ=255)

                                                                                                                                                                                                  cAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCAt                         <  2:898805/239‑1 (MQ=255)

                                                                                                                                                                                                     gTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATAcc                      >  2:1012569/1‑239 (MQ=255)

                                                                                                                                                                                                        cACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTg                   >  1:473897/1‑239 (MQ=255)

                                                                                                                                                                                                          cGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGa                  >  2:644411/1‑238 (MQ=255)

                                                                                                                                                                                                                aTGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCAt           >  2:908882/1‑239 (MQ=255)

                                                                                                                                                                                                                    cgttgacgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCAttttt       >  2:1035274/1‑239 (MQ=255)

                                                                                                                                                                                                                         acgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      <  2:459144/203‑1 (MQ=255)

                                                                                                                                                                                                                         acgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCctc                                      >  1:459144/1‑203 (MQ=255)

                                                                                                                                                                                                                          cgttTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTgggg  <  1:259193/238‑1 (MQ=255)

CGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCT‑GGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGCTGTTTCTGTTATTTATACCTTTGGCTGGGTTTCTGATATTAGCCACCGGGCAAGTTTTAGTGGGTGACCCCTCTGGTTTTCTGCATACCGTGAGCGCCATTTTTTGGGG  >  NC_000913/1478194‑1478647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A