Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,762,268	T→G	22.8%	G29G (GGT→GGG)	rhsA →	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,762,268	0	T	G	22.8%	71.1 / 2.2	60	G29G (GGT→GGG)	rhsA	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base T (18/25); new base G (12/4); total (30/30)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTG  >  NC_000913/3762036‑3762499

tCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGCCAGGGCGACATGACGCAGTATTGCGGACACATTGTTCAGGGTTAAGCCGGGGGGGGC‑CTTG‑Gttcgcc                                                                                                                                                                                                                                             >  2:1272961/1‑233 (MQ=18)

 tttaagagacaacaTTTTTTTCATGGTGATATAAATATTTTCCTAATTATTTCACTTTGATTGATAACACACTTCAGGGTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCa                                                                                                                                                                                                                                           <  1:1032526/228‑1 (MQ=17)

         atCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGACTTGTTCCGGGTTCAGCCGGGGTGCGC‑CTTT‑TGGCCCCCACCGGC‑GTg                                                                                                                                                                                                                                  >  2:461655/1‑239 (MQ=255)

                        tGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTTACCCGGGGTGCGC‑ATTG‑GGGCCCCCACCGGCGGGGGCCTG‑tacctttt                                                                                                                                                                                                                    >  2:1032526/1‑231 (MQ=17)

                        tGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTTCAGGGGTCAGCCGGGGGGCGC‑ATTG‑GTGCCCCCACCGGC‑GGTGCCCG‑TTCg‑ggggg                                                                                                                                                                                                                  >  2:738394/1‑234 (MQ=255)

                                    tagtttCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGc                                                                                                                                                                                                                                                <  2:1040173/196‑1 (MQ=255)

                                     atTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTTCCGGGGTCAGCCCGGGGCCGCGCTTG‑GGCTCCCCCCCGGC‑GT‑GCCTG‑TTccggggggcaccgaggagggg                                                                                                                                                                                                     >  1:896026/1‑219 (MQ=17)

                                        ttCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGAACTGAGGCAGTATGACGGCAGGACATTTCAGGGGTCAGCGCGGGCGCGC‑ATTG‑GGGTCCCCACCGGC‑GTCGCCTT‑TTCGCGT‑AG‑GCCCcggggaggggtgg                                                                                                                                                                                                >  1:872472/1‑229 (MQ=17)

                                           cTAATTTTTTCCCGTTGTTGGATATCCCCCTTTAGGGTTTCTTATAAATTTGTAGGGTTTCGCCGGTCAGACGAAAAATAACCCGATAAAACAAGGAAGAGAAACGAGCGGAAAACCGGCAGCGGGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCCCCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCg                                                                                                                                                                                           <  1:461655/239‑1 (MQ=14)

                                                       gtgtgtacaagagacaggttCAGGCTTTCTTAAAAATTTTTAGGGGTACGCCGGGGAGCAGACAAATAACCCGATAAAACAAGGAAGAGAAAGGGGCGGAAAACCGGCAGCTCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCa                                                                                                                                                                               <  1:277470/221‑1 (MQ=14)

                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGGGC‑ATTT‑TGGCCCCCACCGGC‑GTGGCCCT‑TTCG‑GG‑GT‑GCCCCGGCGGGGTGAAGG‑CCGGCCCTCCGGTCAAAcc                                                                                                                                                                           >  2:996050/1‑238 (MQ=14)

                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGC‑ATTG‑GTGCCCCCACCCGG‑CTGGCCCG‑TTCG‑GG‑TT‑GCCCCGGCGGGGGGGCCG‑CCGGCCCTCCCGGCAATcc                                                                                                                                                                           >  2:677428/1‑238 (MQ=14)

                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCCGGGGGCGC‑ATTG‑GGGCCCCCACCGGC‑GTGGCCTG‑TTTG‑GG‑GG‑GTCCCGGCGGGGTGACGT‑CCGGCCACCCCGTCAATcc                                                                                                                                                                           >  2:238004/1‑238 (MQ=14)

                                                              tgaagtcatgtttgaGGTTTTATTAAAAATCTGGGGGGTGTCGCCAGTACGTCGACCAATAGCCAGATAAAACAAGGATGAGAAATGTGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTAGGGCGGTAGCATTGTGCAGGGTTCAGCCTGGGTGTGC‑ATTG‑GTACCCCCCCCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCt                                                                                                                                                                        <  1:174620/225‑1 (MQ=14)

                                                                  ttcttccTTCAGGTTTTTTTATAAATCTGTAGGGTTTCGCCTGGCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GTCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCt                                                                                                                                                                     <  1:238004/232‑1 (MQ=255)

                                                                  atgtgtataagaggcagcTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCt                                                                                                                                                                     <  2:143372/221‑1 (MQ=255)

                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAGGCAGTATGGCGGTAGCATTGTTCCGGGTTCAGCCGGGGGGGGC‑ATTGAGGGCCCCCCCCGG‑‑GTGGGCTG‑TTGG‑GT‑GG‑GGCCCCGGGGGGGGACgg‑ccggccccccgcggcatcccccccccgg                                                                                                                                                                  >  1:975244/1‑217 (MQ=14)

                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGCCGGGGGGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTTG‑GG‑TT‑GCCCCGGCGGGGGGACGG‑CCGGCCCACCCGGCAAACCCCCGccccg                                                                                                                                                                  >  2:991085/1‑235 (MQ=14)

                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCACTGTTAAGGGGTCAGCCGGGGGGAGA‑CTTG‑TGGCCCCCCCCCGC‑GCGGCCTGTTTGG‑GG‑GT‑GCCCC‑GCGGAGGGGCGG‑CCCGcccccccggccaaccccccccccg                                                                                                                                                                  >  1:1202354/1‑217 (MQ=14)

                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAACAAGGTTCCGGGGTCAAGCGGGGGGGGC‑CCTG‑GTGGCCCCACCCGC‑GGGGGCTG‑TTCG‑GT‑GG‑GCCCCGACGGTGGGGCGC‑CCGGCCCCCCGGGGCATCCCCCGCCC‑gg                                                                                                                                                                 >  1:363002/1‑239 (MQ=14)

                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTTCAGGGGTCAGCCGGGGTGCGC‑CTTT‑GGGCCCCCCCCGGC‑GTGGCCTG‑TTCT‑GG‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCACCCGGGCAATCCCCTGCTC‑gg                                                                                                                                                                 >  2:1272505/1‑239 (MQ=14)

                                                                        cTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑a                                                                                                                                                           <  2:975244/239‑1 (MQ=255)

                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCCTTGTTCAGGGTTCAGCCGGGTTGCGC‑CTTT‑GTGCCCCCACCGGC‑GTGGCCTG‑TTTG‑GT‑GT‑GCCCCCGCGGGAGGACGG‑CCGGCCCTCCGGGCCATCCCCCGCCC‑GGGG‑C‑CAAGGACCTTc                                                                                                                                                 >  2:8589/1‑238 (MQ=14)

                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGC‑CTTG‑GTGCCCCCACCGGC‑GTGGCCCG‑TTCG‑GT‑GT‑GCCCCGGCGGGGTGAAGT‑CCGGCCCTCCGGGCAATCCCCCGCTC‑CGTGCC‑AAAGGGCCtc                                                                                                                                                  >  2:773840/1‑237 (MQ=14)

                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTTCAGGGGTCAGCCGGGGGGGGC‑ATTG‑GTGCCCCCCCCCGC‑GTGGCCGG‑TTCG‑GT‑GT‑GCCCCGGCGGAGGGACGT‑CCGGCCATCCGGGCAAACCCCCGCt                                                                                                                                                                     >  2:277470/1‑221 (MQ=14)

                                                                                            ctttAGGTTTTCGCCGGTCAGCAGACAAAGAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGGCGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAAc                                                                                                                                       <  1:738394/236‑1 (MQ=255)

                                                                                             tGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCACTGTTCAAGGTTCCGCCGGGGGTCGC‑ATTG‑TGGGCCCCCCCGCC‑GGGGGCCG‑GTCG‑GG‑GG‑GGCCCCGCGGGGGGACAG‑CCGGCCCCCCCGGCAATCCCCCGCCCGGGTG‑C‑CAA‑GTGCCTCCCGGGGaaaac                                                                                                                                      >  1:119295/1‑237 (MQ=2)

                                                                                              gTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAACGCGTCAGGGCGACATGACGCAGTATGGCGGGAACAATGTTTAGGGGTCAGCCGGGGTGCGC‑ATTT‑GGGCCCCCCCCGGC‑GGGGCCTGTTTCG‑CG‑GG‑GCCCCGCGGGCTTGACGC‑CCTGCCCACCGG‑CAACCCCCCCCCC‑GGGG‑CGAAAGGCCCCTCCCGGGGAAAc                                                                                                                                       >  1:513032/1‑238 (MQ=9)

                                                                                                      tCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCCGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GG‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGGCCTTTCCGGCGAAACCGACCTCGc                                                                                                                              >  2:36113/1‑238 (MQ=255)

                                                                                                      tCGCCTGGCAGCAAGCAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGcc                                                                                                                             <  2:872472/239‑1 (MQ=255)

                                                                                                         ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTTAAATTTTTTAGGGGTTAGCCGAGAGGCGC‑AACG‑TGGCCCCCACCACG‑GCGGCCCG‑TTCC‑TTGGT‑GCCCGCGCGG‑GGGGGGC‑CCGGCCACGCAGGCACTCCCCTCCCC‑GGTG‑C‑GAAGGGCGTTccctgcggaccccacaacccccc                                                                                                                           >  1:308182/1‑218 (MQ=2)

                                                                                                         ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGGATTGTTCAAGGGTCACCCCGGGGGCGC‑CTTG‑GTGCCCCCAACGGC‑GGGGCCTG‑ATAT‑GG‑GG‑GCCCCCGCGGGGGGTAGG‑CCGGCCATCCCGGACATCCCCGCCTC‑GGGG‑C‑CCAGGGGCTTTCCCGGGAAACCCACACCCCCCt                                                                                                                           >  1:854566/1‑238 (MQ=9)

                                                                                                                 cagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGGATTGTTCAGGGGTCAACCGGGGGGGGC‑AATG‑GTGGCCCCACCGGC‑GGGGCCCG‑GTGG‑GG‑GG‑TCGCCGGCGGGGGGGCGTGCCGGCCACCCGG‑CAACACCCCGCCC‑CGGG‑C‑CAAGGGCCCTCCCCCGGAAACCCCCATCcccccccccccgcg                                                                                                                  >  1:1249435/1‑229 (MQ=2)

                                                                                                                       aaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAACATTGGTCCGGGTTCAGCCGGGGGGCGC‑ATTG‑GTGCGCCCACCCGC‑GTGGCCCG‑TTTG‑GT‑GTGGCCCCGGCGG‑GGGACGG‑CCGGCCCCCCGGCCACTCCCCTGGTC‑GCGG‑C‑CAACGGCCTTCCCCGGGAAACCCACACCCCCCCcccccgccccgcccc                                                                                                            >  1:144063/1‑233 (MQ=9)

                                                                                                                         tAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGt                                                                                                          <  2:101870/239‑1 (MQ=255)

                                                                                                                                aTAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGGGGGACGT‑CCGGCCATCCGGTCAATCCCCTGCCC‑GGGG‑C‑CAAGGTCCTTCCCGGTGAAACCGACATCGCCCTCCCCGGCCCCCTCCCGTTCATcc                                                                                                    >  2:642777/1‑238 (MQ=14)

                                                                                                                                     aCAAGGATGAGAAATGAGCGGAAATCCGGCAGCGCGTCAGGGCCACATGGCGCCGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTccc                                                                                              <  2:939222/239‑1 (MQ=255)

                                                                                                                                     aCAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCGGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTccc                                                                                              <  2:854566/239‑1 (MQ=255)

                                                                                                                                            tGAGAAATGGGCGGAAACCCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  2:1202354/239‑1 (MQ=255)

                                                                                                                                             tggaAATGGGGCGAAAACCGGCGGCGCGTCAGGGGGGCGTTGCGGGGTGTGGCGGTAGCCTTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  1:642777/236‑1 (MQ=11)

                                                                                                                                             gggaAATGGGCGGAAAACCGGCGGCGGGTCGGGGGGACATGCCGCAGTAGGGCGGTGGCATTGTTCAGGGTTCAGCCGGGGGGCGC‑ATTG‑GGGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  1:1272505/236‑1 (MQ=11)

                                                                                                                                             gaggAATGAGCGGAAAACCGGCAGCGCGTAAGGGCGACAGGACGGAGTTTGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  2:571900/238‑1 (MQ=255)

                                                                                                                                                 aaTGGGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GCGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTAcagc                                                                                   <  2:119295/238‑1 (MQ=25)

                                                                                                                                                                     cgcgTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGcc                                                               <  2:582885/238‑1 (MQ=33)

                                                                                                                                                                        gTCAGGGCGACATTCCTCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCcgcg                                                           <  2:477454/239‑1 (MQ=18)

                                                                                                                                                                                 aaaTGACGCAGTATGGCGGTAGCATTGTTCGGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTggg                                                   <  2:129534/236‑1 (MQ=17)

                                                                                                                                                                                  cATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTgggg                                                  <  2:248647/238‑1 (MQ=32)

                                                                                                                                                                                      aCGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATGG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGGTACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGcc                                              <  2:886311/238‑1 (MQ=17)

                                                                                                                                                                                           gTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcc                                        <  2:1277338/239‑1 (MQ=32)

                                                                                                                                                                                                          tGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GGGCCCCCCCCGGC‑GGTGCCTG‑GTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCCGCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGc                         <  2:144063/239‑1 (MQ=255)

                                                                                                                                                                                                                 ggTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCCACTACAGCAGGTACCGGCCAAAAACGCCCGCGCCGGTGGGGAGGCTCGGCCCCCGCCGGAAAAAGCCAGCGGa                  >  1:62470/1‑239 (MQ=11)

                                                                                                                                                                                                                    tCAGCCGGGGTGCGC‑ATTG‑GGGCCCCCCCCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:497566/239‑1 (MQ=17)

                                                                                                                                                                                                                     cAGGCGGGGTGCGC‑ATTG‑GGGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:1031017/238‑1 (MQ=17)

                                                                                                                                                                                                                     cAGCCGGGGTGCGC‑ATTG‑GTGCCCCCCCCGGG‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:707827/238‑1 (MQ=17)

                                                                                                                                                                                                                      cggcGGGGAGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGCCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑CGTG‑C‑AAAGGGCCTTCCCGGTGAAACCGACATCCCCCGGCCCGGCCCGCTGCCGTTCATCCTCCCCCGCACCTACACCAGTTAACGGGCAAAACCACCCGCGCCGGCGGGGGGCCTGCGCCCCGGCCGGcaaaaacacgcggccagcc             >  1:18147/4‑224 (MQ=11)

                                                                                                                                                                                                                      aGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACCAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCGGGGAAATGCCTGCGGACATcc             >  2:551815/1‑239 (MQ=12)

                                                                                                                                                                                                                           gggTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCCCCCCCACCCACCGCCGTTACCGGCCAAAAACGCCCGCGCCGGCTGGGGGCCCCGCCCCCGCCCGGCAAAtacaccggcacacacgccg         >  1:1211424/1‑220 (MQ=11)

                                                                                                                                                                                                                           gggTGCGC‑ATTG‑GTGCCCCCACAGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCCTCCGGCCAATTCCCTGCTC‑GGGG‑C‑AAAGGTCCTTACCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCCGTTACCGGCAAAAAACGCCCGGCCCCGCGGGGGGCTCCGGCCCCCGTCGGaaaaaagcaccggagaacccctgc        >  1:101793/1‑219 (MQ=11)

                                                                                                                                                                                                                             gTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGCCAAAAACGCCCGCCCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTCCGGATATCCGCTTAc       >  2:580260/1‑238 (MQ=17)

                                                                                                                                                                                                                                 gc‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg  <  2:363002/239‑1 (MQ=11)

                                                                                                                                                                                                                                  c‑ATTG‑GTGCCCCCACCGGC‑GGGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg  <  2:36264/238‑1 (MQ=11)

TCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGC‑ATTG‑GTGCCCCCACCGGC‑GTGGCCTG‑TTCG‑GT‑GT‑GCCCCGGCGGAGTGACGT‑CCGGCCATCCGGTCAATCCCCTGCTC‑GGTG‑C‑AAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTG  >  NC_000913/3762036‑3762499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A