Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,632 A→C 15.1% V448G (GTG→GGG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,6320AC15.1% 331.5 / 4.2 140V448G (GTG→GGG) cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (64/46);  new base C (2/28);  total (66/74)
Fisher's exact test for biased strand distribution p-value = 1.92e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCC  >  NC_000913/4103395‑4103862
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGACGTTTAACTCCGCTTATACAGCGGCAAACAAAacac                                                                                                                                                                                                                                          >  1:823068/1‑239 (MQ=255)
ccccggtggCCGGGTTACCGAAACGCGGGGCGCGGACGATGCAAAAGCGCC‑GACGGAGCGGCAAGATCGAAGATTTTTCC‑CGGCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                          <  2:244475/231‑1 (MQ=39)
   ccGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGCGCGGCAAGATCGACGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGGGTA‑GGCCTGCTAAGACGCTATCAGCGGCGCCTCAGGCATCCTGCTCAAAGGCCGC‑TTGCGGCGCAAAGGCCTACTCCTGCCTGCAAATGCGAAGTTTAACTCCCCTTAAGACGCGGCAACAAAACAACCa                                                                                                                                                                                                                                        >  1:158531/1‑238 (MQ=255)
    cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGCTAAGACGCTATCAGCGTCGCATCAGGCCTCCTGCTCAAATTCCGG‑ATGCGGCGTAAACGCCTTATCCGTCCTGCAAATGCGAAGTTTAACTCCGCTTATACAACGTCAAAAAAAACACCaac                                                                                                                                                                                                                                      >  1:43901/1‑237 (MQ=255)
            ggTTATCAACAGGCGGGCCGCGACTGATGCAAAA‑CGCCAGACTGGACGGCAGGTACAAGGTTTTTCCC‑CGCCGGTTGTGGGAAAAAAACCCCCCGGAGTGTG‑GGCCTGAAAAGCCGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑cc                                                                                                                                                                                                                             <  1:181833/239‑1 (MQ=255)
                      aggCTGGACGCGGAAGATGCAAAAGCGCC‑GACTGGAGGGCAAGATCGAAGGTTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCggg                                                                                                                                                                                                                   <  2:279693/237‑1 (MQ=255)
                      aaGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCggg                                                                                                                                                                                                                   <  2:177512/239‑1 (MQ=255)
                      aaGCTGGACGCGGACGAGGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCggg                                                                                                                                                                                                                   <  2:1274070/239‑1 (MQ=255)
                       aGCGGGCCGGGGACGATGAAAAGGCGCC‑AACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTGGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGc                                                                                                                                                                                                                  <  2:1291220/239‑1 (MQ=255)
                        gCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGGAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCATCCGTAAA‑CCGCCCAGCGGGcc                                                                                                                                                                                                                 >  2:181833/1‑238 (MQ=255)
                                  gACGATGCAAAAGCGCC‑GACTGAGCGGCCAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGCGTGTA‑GGACTGATAAGACGCTATCCGCGTCGCATCATGCATCCTGCTCAAATGCCGG‑GTCCGGAGTAAACCCCTTATCCTGCCTTCAAATGCGAAGTTTAACTCCGCTTAAACAGCGGACACCAAACAACCAGCCGTAAC‑CCGACCAGCGAGCTGccttcgc                                                                                                                                                                                                         >  1:806725/1‑235 (MQ=255)
                                  gACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTg                                                                                                                                                                                                         >  2:982191/1‑237 (MQ=255)
                                                gcC‑GACTGGGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGGGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCa                                                                                                                                                                                           <  2:1224731/237‑1 (MQ=255)
                                                atgttgataagagcacggATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCa                                                                                                                                                                                           <  2:1051117/221‑1 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCTGCCTTCTGCCTTCACCCAGCCACGa                                                                                                                                                                                        >  2:1210225/1‑239 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCTGCCTTCTGCCTTACCCCAGCCACGa                                                                                                                                                                                        >  2:1245370/1‑239 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATACCCACCCGACAA‑CCGCCCACCGGGCTGTCCTCTGCCTGCCCCCACCCCCgc                                                                                                                                                                                        >  1:749910/1‑238 (MQ=39)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGAAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGCCTTCTGCCTTCACCCAGCAACGa                                                                                                                                                                                        >  2:565133/1‑239 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTCCACATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAACCCTAAA‑CCGCCCAGCGGGCTGCCTTCTGCCTTCACCCCGCCACgc                                                                                                                                                                                        >  1:990587/1‑238 (MQ=255)
                                                    gcatggggggcaagaggtaggATTTGCCC‑CGCCGGTGGTGGGAAAAATAA‑CCCCGGGGTGGAGGGCCTGATAGGACGCTATTGCCGTCGCAACAGGCATCCTGCTCAAATGCCGA‑ATCCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtg                                                                                                                                                                                      <  1:882390/219‑1 (MQ=255)
                                                     aCTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGGCTTCTGCCTTCACCCAGCCACGAtgc                                                                                                                                                                                     >  2:750963/1‑239 (MQ=255)
                                                       tgggCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg                                                                                                                                                                                   <  2:170938/236‑1 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtgcg                                                                                                                                                                                    >  2:1001428/1‑235 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCTGTCTTCTGCCTTCACCCAGCCACGATgctgct                                                                                                                                                                                 >  2:617383/1‑239 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCAGTCTTCCGCCTTCACCACCCCACAAtgccgcc                                                                                                                                                                                 >  1:79790/1‑238 (MQ=39)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CACCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtgct                                                                                                                                                                                    >  2:931456/1‑236 (MQ=255)
                                                                   ccgaaagttttcCC‑CGCCGGTTGTGGGGAAAATAACCCCGGGAGTGTA‑GGCCTGATAAGACGCTAGCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGt                                                                                                                                                                       <  2:569500/232‑1 (MQ=255)
                                                                       ggATTGTTCCGCGCGGGTGTTGGGGAAAAACCCCCCCGGGGGGGA‑GGCCTGATGAGACGCGATGTGCGTGTCCTCAGGGCGCCTGGTCAATTGCCGG‑TTGCGGCGGAAAAGCATTATCCGTCCAGCAAATGCGAAGTTTAACTCCGCTTATACAGGGGCAACCAAAACCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCTCCCAGCCACGATGCTGCTGAATGGCGGttt                                                                                                                                                                     <  1:898943/237‑1 (MQ=255)
                                                                             ttCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCCAATCACCAGCCGTAAA‑CCGCCCCGCGGGCTGTCTTCTGCCTTCACCCAGCCACCATGCTGCAGCAGGGCGGGTTccaaaaa                                                                                                                                                              >  1:741723/1‑232 (MQ=39)
                                                                                cccgagggTTGGGGGGAAAAGAACCCCGGGAGTGTA‑GGCCTGAAAAGACGCTAGCAGCGTCGAATCAGGCATCCTGCCCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGc                                                                                                                                                           <  2:158531/233‑1 (MQ=255)
                                                                                c‑cgccgGTTGTGGGGAAAATAACCCCCGGGGTTGA‑GGCCTGATAAGACTCTACCAGCGTCGCACCAGGGTACCTGCTAAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGcc                                                                                                                                                          <  1:1245370/239‑1 (MQ=255)
                                                                                 ccccctttttgggggAAAAAACCCCCCGGGTTTTT‑GGCCGGATAAGACCTTATCGGCGTCGTATCAGGCATGCTGCTAAAATGCCGG‑AGGCGGCGTAAACGCCTTACCCGGCCTGCAAATGCGAAGTTTACCTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGcc                                                                                                                                                          <  1:551185/228‑1 (MQ=255)
                                                                                    ccgGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGCCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGGGGGTTtaacaaatcccac                                                                                                                                                        >  2:1027918/1‑231 (MQ=255)
                                                                                      gtttGGGGGGAAAATTACCCCCGGGTTTTA‑GGCCTGGAAAGAGGCTATCAGCGTGCCATCCGGCATCCTGCTCAAAGGCCGG‑ATGCGGCGTAAACGCCTTAACCTGCCGGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAACCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGccc                                                                                                                                                     <  1:544303/237‑1 (MQ=255)
                                                                                                     tccccccGGAGTTTG‑GGCCTGAAAGGGCCGTTTCCGCTCCGGATCGGGCGTCCTGCTCAAAGGCCGG‑ATGCGGCGAAAACCCCTTACCCTGCCTGCAAAGGCGAAGTTTAACCCCGCTTATACGGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAACCCTGTACCGcc                                                                                                                                      <  1:104124/237‑1 (MQ=255)
                                                                                                     accccccGGGGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACCCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc                                                                                                                                      <  2:1209540/237‑1 (MQ=255)
                                                                                                     accccccGGAGTGTA‑GGCCTGAAAAGACGCTATCAGGGTCGAATAAGGCATCCTGCTCAAATGCCTG‑ATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc                                                                                                                                      <  2:297131/237‑1 (MQ=255)
                                                                                                       cccccGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGGTTTAACAATACCCAGCCCCCAAcccttaaccccca                                                                                                                                    >  2:773109/1‑228 (MQ=39)
                                                                                                            ggAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATACCCAGCCCCAAACCTGTACCCCCCGAttt                                                                                                                                >  2:880692/1‑237 (MQ=255)
                                                                                                                tgtA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCATTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAAAAATAGCCAGCCCCAAACCTGTACCGCCACATTCaagg                                                                                                                            >  2:953938/1‑236 (MQ=255)
                                                                                                                tgtA‑GGCCTGATAAGACGCTATAAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCTGCTGAATGGCGGGTTCCACAATCGCCCGCCCCCCACCCGTACcccccacttaacca                                                                                                                            >  2:1325732/1‑228 (MQ=255)
                                                                                                                  tA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGGTc                                                                                                                          >  2:511989/1‑238 (MQ=255)
                                                                                                                  tA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGGTTCAACAATCGCCAACCCCCAAcccataaccccaaatacacaaacg                                                                                                                         >  1:1320298/1‑217 (MQ=255)
                                                                                                                      gcgtGATAAGCCGCTATCAGGGGCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATGCACGATcgcg                                                                                                                       <  2:252232/235‑1 (MQ=255)
                                                                                                                           aTAAGGCGCTATCAGCCGCGCATCCGGCATCTTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTCTCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTc                                                                                                                 <  2:1264142/239‑1 (MQ=255)
                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTTCCTTCACCCAGCCACGATGCTGCTGAATGGGGGTTTCCACAAACGCCCGCCCCCAACACGCCACGCCCGATTCACGCACGCGTGCtt                                                                                                                  >  1:1128557/1‑238 (MQ=255)
                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCCACAATCGCCAGCCCCAAACCTGTAACGCCAGATTCACGATACCGGGCTTc                                                                                                                 >  2:3089/1‑239 (MQ=255)
                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCCAACCCGTACCGCCAGATTCACGATCGCGGGCtt                                                                                                                  >  2:292235/1‑238 (MQ=255)
                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCCGTAACGCCAGGTTCACGACcgccgtgttc                                                                                                                 >  2:987566/1‑232 (MQ=255)
                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGGTTTAACAAATCCCAACCCCAAACCCTAAACGCCCAATTCACAGTCGCGGGCttt                                                                                                                 >  2:517010/1‑238 (MQ=255)
                                                                                                                             aaGACGCTATCAGCGTCGCATCAGGCATCCTGCTAAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                               <  2:79790/239‑1 (MQ=255)
                                                                                                                                gcGCTACCCGCGTCGAATCGGGCCCACTGCACAAATGCCGT‑ATGCGGAGAAAACCCTTTATCCGCCCGGCAAATGGAAAGTTTACCGCCGCTTAGCCAGCGGCAACAAAACCCCCGGCCGTAAA‑CCGCCCAGCGGGCTGTTTTCTGCCTTCGCCCAGCAACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAATCTGTACCGCCAGATTCGCGATCGCGTGCTTCATCgg                                                                                                            <  1:583380/238‑1 (MQ=255)
                                                                                                                                  gCTAACAGCGGCGCATCAGGCATCCTGCTCAAAGGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCgg                                                                                                            <  2:823068/237‑1 (MQ=255)
                                                                                                                                    tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGcc                                                                                                                                                          <  2:1144970/189‑1 (MQ=255)
                                                                                                                                    tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCCCGCACTTCCACCGTACg                                                                                                        >  2:274644/1‑239 (MQ=255)
                                                                                                                                     aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGAACCGCCCGATTCAAGATCCCGGGGTTCATCGGTCCG‑a                                                                                                      >  2:168911/1‑239 (MQ=255)
                                                                                                                                     aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCACAACCCGTACCGCCAGGATCCCGATCCCGTGCTTCATCGGTACG‑a                                                                                                      >  2:41893/1‑239 (MQ=255)
                                                                                                                                     aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGATTCAACCATCGCCAGCCCCAAACCCGTACCCCCAGATTCACCATCGCCTGCTTCCTCGGGAccg                                                                                                       >  2:481740/1‑237 (MQ=255)
                                                                                                                                           gTCGCAGCAGGCACCCTGCTCAAATGCCGG‑GTGCGGCGGAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGa                                                                                                  <  2:291755/237‑1 (MQ=255)
                                                                                                                                            tCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCCAAACTGTACCCCCCGATTCACGATCCCGTGCTTCACCGGTACG‑ATAGGac                                                                                                >  2:724728/1‑238 (MQ=255)
                                                                                                                                              gCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAGCTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGc                                                                                                                                                                                                                      >  1:489395/1‑119 (MQ=255)
                                                                                                                                              cccccgggCATCCTGCCCAAA‑GCCGGTGTGGGGCCTAAACGCCTTAGCCTGCTGGCAAAGCCGAATTTTATCTCGGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTTAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAacgga                                                                                             <  1:481740/233‑1 (MQ=255)
                                                                                                                                                        tCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATc                                                                                    <  2:384003/238‑1 (MQ=255)
                                                                                                                                                          ccgttCAAATCCGGG‑TTGGGGGGAAACCCCCTTACCCCGCCCGCAAATGGCAAGTTTAACCCCGCTTTTCACGCGGCAACCAAATCACCAGCCGGAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGt                                                                                 <  1:1001428/235‑1 (MQ=255)
                                                                                                                                                           ttcaggtgtttataagtgtcaggTAAACGCCTGATCCTGCCTCAAAATGGCATGTTTAACCCCGGTTCACCAGCGGAAACAAAACCACCAGCCGTAAA‑TCGCCCAGCGGGGTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGTCGGTTTCAACAATCCCCATCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATc                                                                                    <  1:330741/214‑1 (MQ=255)
                                                                                                                                                              tCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCtttttcc                                                                             >  2:31607/1‑236 (MQ=255)
                                                                                                                                                                 aaTGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCCAACCTGTACCGCCAGGTTCCCGATCGCGTGCTTCATCGGTACG‑ATAGAACCGACGGAAAAACTGTTcccgg                                                                           >  2:1142808/1‑237 (MQ=255)
                                                                                                                                                                  aTGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCTACACTCCCCAGCCCCAAACCTCAAACCCCCGATTCACGCTCTCCTTCCTCCTCACGACG‑AAAAAACCGACGGaaaaacgttttcagga                                                                          >  1:667830/1‑226 (MQ=255)
                                                                                                                                                                            ggggggtaaccccttttccctccctgAAAATGCGAATTTTAACCCCGCTTATACCGCGGCAACCAAACCCCCAGCCGTAAA‑CCCCCACGCGGGCTGTCTTCCGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt                                                                <  1:982282/221‑1 (MQ=255)
                                                                                                                                                                            gcggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt                                                                <  2:1320298/239‑1 (MQ=255)
                                                                                                                                                                              gggcaaaaCCCCTTACCCTGCCTGAAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑a                                                              <  2:479308/233‑1 (MQ=255)
                                                                                                                                                                                   aaaCGCCTTATCCTGGCTGAAAATGCGAAATTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGcc                                                         <  2:863018/238‑1 (MQ=255)
                                                                                                                                                                                       gCCTTCTCCTGCCTGCAAAGGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGAc                                                    <  2:43901/239‑1 (MQ=255)
                                                                                                                                                                                          ttATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGCATGGCGGGTTTAACAAACCCCCGCCCCCAACCCCAGACCCCCCAGTCACCACAGCGGGCTTCACCGCTACC‑ATAAAACAGACCGAAAAAATGTTCCCGCACTTCCGGGCTCAACGCCAgaaaa                                                   >  1:647476/1‑234 (MQ=255)
                                                                                                                                                                                            aTCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATAGCCAGCCCCAAACCCGTACCCCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAAAAAGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCaacc                                               >  2:1245978/1‑236 (MQ=255)
                                                                                                                                                                                            aTCCCGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                               <  1:25359/177‑1 (MQ=255)
                                                                                                                                                                                                 gcctgaAAATGCGAAGTTTACCCCCGCTAATACAGCGGCAACCAAACCCCCAGCCGTAAA‑CCGCCCAGGGGGCTGTCTTCTGCCTTCCCCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCgtcgtc                                          <  2:1048077/239‑1 (MQ=255)
                                                                                                                                                                                                 ccctcaaaaCGCGAATTTTCCCCCCGTTTATAGCGCGGCAACCAAACCCCCCGCCGTAAA‑CCCCCCAGGGGGTTTTTGTCGCCCTTCCCCCAGCCACGATGCTGCGGAATGGCGGTTTCAACAAGCGCGAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCgtcgtc                                          <  1:1247397/233‑1 (MQ=255)
                                                                                                                                                                                                  cctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                                                                                                                                                                                                                          >  2:337883/1‑49 (MQ=255)
                                                                                                                                                                                                  cctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTTAACAATCGCCAACCCCCAACCTTGACCCCCAGAGTCACGTACGCGTGCTTCCTCGGTACGCAAAAAACGGCCGGAAACCCTTTTC‑CGCACTTCCGCGCT‑AACGcccgagaacccgtcggc                                          >  1:380418/1‑223 (MQ=255)
                                                                                                                                                                                                   ctgcAAATTCGAAGTTTAACCCCGCTTAAACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCa                                        <  2:586220/239‑1 (MQ=255)
                                                                                                                                                                                                   ctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTTCTTCATCGGGACG‑ATAGAACGGACGGAAAAACTGTTCGCGAGCTTCCCGGCT‑AAAGCCAGGACCATCGGCGTcc                                         >  2:1324877/1‑238 (MQ=255)
                                                                                                                                                                                                   ctgcAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAAACTGTACCGCCAGATTCACGATCGCGTGCTTAATCGGTACG‑ATAGAACGGACGGAAAATATGTTCGCGATCTTCCGGGGT‑AACGCCAGGACCACCGCCGTcc                                         >  2:279423/1‑238 (MQ=255)
                                                                                                                                                                                                   cgccAAATGCGAAGTTTACCTCCGCTGATCCAGCGCCAACCAAACCCCCAGCCTTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAAGCGCCACCCCCAAACCTGGACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCa                                        <  1:3089/236‑1 (MQ=255)
                                                                                                                                                                                                       aaaTGCGAATTTTAACCCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGt                                    <  2:631456/239‑1 (MQ=255)
                                                                                                                                                                                                       aaaTGCGAAGTTTAACCCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGt                                    <  2:315191/239‑1 (MQ=255)
                                                                                                                                                                                                                 tttACCCCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                          <  2:1028088/239‑1 (MQ=255)
                                                                                                                                                                                                                  tttcctcgtcttttccAGCGGAACACAATTCCCCAGCCTTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGGTTAATTGCGGTTTCAACAATCGCCAGCCCCAAACCTTTACCGCCAGATTCACGATCGCGTGCTTCATCGGGACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                          <  1:1156679/223‑1 (MQ=255)
                                                                                                                                                                                                                  ttAACTCCGGTTATACAGGGGCAACCAAACCCCCAGCCGTAAA‑CCGCCCAGCGGGCTTTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                          <  2:476902/238‑1 (MQ=255)
                                                                                                                                                                                                                  ttAACCCCGCTTATACAGCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATAc                         <  2:809257/239‑1 (MQ=255)
                                                                                                                                                                                                                  tcaaCCCCGTTTATACCGCGGCACCAACATCACCTGGCGTAAA‑CCGCCCGGCGGCGTGTTTTCTGCTCTACCCCAGCGAAGATGCGTCTGAATGGCGGTTTCAACAAGCGCCCGCTCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAGCGGTACG‑ATAGAACGGACGGAAAATCCGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATAc                         <  1:1325732/237‑1 (MQ=255)
                                                                                                                                                                                                                           cTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCCAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCTGCGTTCACCGTCAGGGGGATAACGCCttttt                >  2:833983/1‑237 (MQ=255)
                                                                                                                                                                                                                             taacacgCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCGGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCt              <  2:13165/233‑1 (MQ=255)
                                                                                                                                                                                                                                   gCGGCACCCACACCCCCCGCCGAAAC‑CCCCCCAGGGGCCTGGCTTCCGCCCCCCCCCAGCCCCGGGGCCGCTGAAAGGCGGTTTCAACCATCGCCAGCCCCAAACCCGTACCGCCAGATTCCCGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  1:28178/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cgcgcgcccAAATCCCCCGCCGTAAA‑CCCCCGCGGGGGTTGTCTTCCCTCTCCCCCCGCCCCCTGTCCTGCTGAATGGCGGTTTCAACAACCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  1:1008828/232‑1 (MQ=255)
                                                                                                                                                                                                                                   ccgctaaCAAAACCCCCACCCGCAAC‑CCGCCCAGGGGGTTGTCTCCTGCCTCCACCCAGCCCCGCTGCTGCGGAATGGCGGTTTCAACAATCGCCAGCCCAAAACCTGTCCCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  1:308723/234‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAAACTGTTCGCGATCTTCCCGGCT‑AACCCCCGGACCATCGTCGTCCACCCTAATGGGGATACCGTCTTTATCTACCGcca       >  2:87726/1‑237 (MQ=255)
                                                                                                                                                                                                                                    cGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCCTGCTTCATCGGTACG‑ATAGAACGGACGGAAAAACTGTTCGCGATCTTCCCGGCT‑AACGCCAGGCCCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCaa       >  2:1073948/1‑239 (MQ=255)
                                                                                                                                                                                                                                     ggCACCCAAACCCCCAGCCGAAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  2:380418/237‑1 (MQ=255)
                                                                                                                                                                                                                                         aCCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCCCGGTCTTCCGGGCT‑AACCCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTTTCTACCCCAAAGccc  >  2:308723/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
TCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTA‑GGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTGAATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACG‑ATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCC  >  NC_000913/4103395‑4103862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A