Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F9 I0 R1	260	102.5	2178490	93.2%	2030352	226.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,665,654	T→A	15.6%	V52V (GTA→GTT)	gadX ←	acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,654	0	T	A	15.6%	206.9 / 3.2	76	V52V (GTA→GTT)	gadX	acid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand): ref base T (36/26); new base A (3/10); total (39/37)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTA  >  NC_000913/3665419‑3665869

aGTTCTTATCCTCAAGGAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACa                                                                                                                                                                                                                         <  1:284709/239‑1 (MQ=255)

    cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAAAAAAAAAt                                                                                                                                                                                                                     >  2:741969/1‑239 (MQ=255)

    cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GAATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGAAACGAAAAAAAAAt                                                                                                                                                                                                                     >  2:611928/1‑239 (MQ=255)

     tCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAAtt                                                                                                                                                                                                                    >  1:802651/1‑239 (MQ=255)

       aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACCCGAATAAAAAAtttt                                                                                                                                                                                                                  >  2:454699/1‑239 (MQ=255)

       aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAAtttt                                                                                                                                                                                                                  >  1:680678/1‑239 (MQ=255)

         ccTTAAAAAAAACAGATAACCACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Ca                                                                                                                                                                                                               <  2:220788/239‑1 (MQ=255)

          ctaaaGAAAAACGGATTACAACGCAAAGATTAATGCTTTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:202328/236‑1 (MQ=255)

          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:353468/239‑1 (MQ=255)

          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  1:950830/239‑1 (MQ=255)

          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:994064/239‑1 (MQ=255)

          cTCAAGAAAAACAGAAAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:941068/239‑1 (MQ=255)

            cAAAAAAAACAGATAACAACGCAAAGATTAATGCTCGTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAaca                                                                                                                                                                                                            <  1:41471/239‑1 (MQ=255)

            aaaaaaaaaaAGAGAACAACGAAAAGATTAATGCTTTTTTACGTAGCATGTCTGCATAGCGAAGATTCTTATTCGGCGATAGTTGCGCAAATTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAaca                                                                                                                                                                                                            <  1:393799/235‑1 (MQ=255)

                    aCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATc                                                                                                                                                                                                    <  1:366399/239‑1 (MQ=255)

                       gATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGCATACAAAATTTT‑CAACACACTTATCTa                                                                                                                                                                                                  >  1:862976/1‑238 (MQ=255)

                             aacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGGGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAAt                                                                                                                                                                                           >  2:619435/1‑239 (MQ=255)

                              acGAAAAGATTTATTCTTTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAAtt                                                                                                                                                                                          <  2:17730/239‑1 (MQ=255)

                               cgaaaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:541675/215‑1 (MQ=255)

                               cGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              >  1:541675/1‑218 (MQ=255)

                               cGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAAttt                                                                                                                                                                                         >  1:834443/1‑239 (MQ=255)

                                  aaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTg                                                                                                                                                                                        >  1:412217/1‑237 (MQ=255)

                                         aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCAt                                                                                                                                                                               >  1:874008/1‑238 (MQ=255)

                                            gCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGc                                                                                                                                                                           <  1:892092/239‑1 (MQ=255)

                                             cttttttTCGTTACATTTTTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCa                                                                                                                                                                          <  2:874008/236‑1 (MQ=255)

                                                ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACCCGAATTTGGC‑TTGCATCC‑Caaaaa                                                                                                                                                                      >  2:745493/1‑239 (MQ=255)

                                                             ttCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCa                                                                                                                                                                          <  1:933306/223‑1 (MQ=255)

                                                                    tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTaaa                                                                                                                                                  >  2:857306/1‑239 (MQ=255)

                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATa                                                                                                                                               >  1:52086/1‑239 (MQ=255)

                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCCCAAAAACCAGGA‑CACCGCCCTTaaaaaa                                                                                                                                               >  2:427008/1‑237 (MQ=255)

                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAATTTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGCCCCGAAAACAAACTTTC‑CAAAACAATTACCTACCCAAATTTGCC‑TTCCATCGCCAAAACCCAGGT‑ccggtctcttaaaaac                                                                                                                                               >  2:933306/1‑224 (MQ=255)

                                                                          aGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGCCACGAATACAAAATTTT‑CAACACACTTATATACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAAAAGCg                                                                                                                                            >  2:988145/1‑239 (MQ=255)

                                                                                 tATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATATCGCTATTc                                                                                                                                      >  1:414666/1‑238 (MQ=255)

                                                                                    tCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAAAAGCGATATTCACCa                                                                                                                                  >  2:155512/1‑239 (MQ=255)

                                                                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTAt                                                                                                                                                                                                     <  2:613264/172‑1 (MQ=255)

                                                                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTAt                                                                                                                                                                                                     >  1:613264/1‑172 (MQ=255)

                                                                                              ttGCGCACTTTCATAAAAACCAGTAATTTATCTACAAAAGTGAGAAACAATAAAGGAAGAAATTTTTTTA‑TGT‑GCATGAAGATTTAAGGCCTCCTCCTGA‑ACCACCGGGAAAAAAAATAAAAG‑CGTGT‑CCTTTGACACAAAAACAAAATTTT‑CAACACTCTTATTGACTGAATTTTGGC‑TGGCACCCGAAAAACCAAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                                        <  1:92010/239‑1 (MQ=255)

                                                                                                gctcAATTTCCTAAAAAAGAGAAATCCCCATACTACAGTGTAGAACGATTAAAGAAGAATCTTTTTTA‑GGT‑GCAAGAATTTTCAATGCCCCCTCCTGG‑ACCATCGGAAAAAATAATGAAGG‑CTTTG‑CCCTTGACACAAATACAAATTTTT‑CAACACTCTTATCTACTCGATTTTGGC‑TTCCTACCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                                      <  1:611928/236‑1 (MQ=255)

                                                                                                    catctccaaaaaaacgaaaaTTTCTCTACAAGAGTGAAGAACGAGAAATGAAGAATTTTTTTTA‑GGT‑GAATAAAGATTTAATGCCCCCTCCGTG‑AACATGGGGAAAAATAATGAAAG‑GGTGT‑CCTTTGACAAAAAAAAAAATTTTT‑CAACACACTTTTCTCCTCGATTTTGGC‑TTGCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                  <  1:267698/221‑1 (MQ=255)

                                                                                                    aatttCTTGAAAAATAGGTATATTTTTTCTACAGTGATGAAGGATTAATAAAGAATCTTTTTTA‑TGT‑GCATAAAGATTTAATGCCTCCTCATGG‑ACAAGCGGAAAAAATAAGGAAGG‑CTTGT‑CCCTTGAAACAAATAAAAAATTTC‑AAACACACTTTCCTACTCAATTGTGTT‑TTGAACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                  <  2:412217/236‑1 (MQ=255)

                                                                                                      tttcataaaaaaCAGGAATATTTCAACTAAGGTGAGAAACGAGTAATGAA‑AAACTTTTTTATTGT‑CAAGTAAGTTTTAATCCCTCCACTTAG‑AGCATGGGAAAAAAAAATGAAGG‑CGTTT‑CCCTTGACAAAAAAAAAAATTTTT‑AAACCCTTTTATCTATTCAATTTTGGC‑TTGCCTCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                                                                                                                <  1:754531/232‑1 (MQ=255)

                                                                                                                    ggAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGGGAGAATATATTTATGTCTTGc                                                                                                  >  1:570064/1‑239 (MQ=255)

                                                                                                                       tttttcctctcaaggtaagaaaCGAGTAA‑AAAAAAACTTTGTTG‑TGTGGAATAAAATTTTATTCCCCCCTCCTGG‑AGCGCGGGAAAAAAAAATAAAGG‑CGGGC‑CCTTAGCCACAAAAACAAATTTTC‑AAACCCAC‑TCTCTAACAAAATTTGGCTTTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                               <  2:691667/219‑1 (MQ=255)

                                                                                                                       atctctaTACTAGAGTGAGAAACGAGAAATAAAAAAACTTTTTTA‑GGT‑GAATGAATTTTAAATGCCTCCCCCGGA‑AGCATGGGGAAAAATAATGAAAG‑CGTTC‑CCTTTAACAAAAAAAAAAATTTTC‑CAACACACTTATCTACTAGATTTTGGC‑TTGCATCCACAAAAACGAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                               <  1:745493/236‑1 (MQ=255)

                                                                                                                             ctactaCAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAATACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                         >  1:139364/1‑239 (MQ=255)

                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:129821/115‑1 (MQ=255)

                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              >  1:129821/1‑115 (MQ=255)

                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCAc                                                                                                                                    >  2:70631/1‑187 (MQ=255)

                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                                         >  2:58603/1‑214 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATAtt                                                                                                                                       >  1:927945/1‑182 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCCTTAACCATGGTGAGAatatat                                                                                                             >  1:519470/1‑208 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                             >  1:586212/1‑208 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                               >  1:277882/1‑238 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCAAACGCAATTAAAAAATTc                                                                               >  2:764034/1‑238 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCAATTAAAATAGCGATATTCACCa                                                                                                                                  >  1:242928/1‑187 (MQ=255)

                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCACAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                               >  1:839073/1‑238 (MQ=255)

                                                                                                                                                      aagaaACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              >  1:785756/1‑99 (MQ=255)

                                                                                                                                                      aagaaACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑Caa                                                                                                                                                                                                              <  2:785756/99‑1 (MQ=255)

                                                                                                                                                         aaaCTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATgaa                                                                        >  1:664166/1‑226 (MQ=255)

                                                                                                                                                           ttttttttA‑TGT‑GCATAAATATTTAATCCCTCCTCGTGG‑AGCGTC‑GGAAAAAAAAAGAAAGTCGTGC‑CCTTTGAACCAAAAAAAAAATTTT‑AAACACACTTATCTACTCAATTTTGGT‑TGGCACCCGAAAAACCCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:973589/237‑1 (MQ=255)

                                                                                                                                                           ttttttttA‑GGT‑GCAAGAATTTTTAAGCCCTCCTCCTTG‑AGCACGGGAAAAAAAAATAAAAG‑CTTGC‑CCCTTGACACAAATAAAAAATTTA‑CAACACACTTATCTACTAAATTTTGGC‑TTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:145811/237‑1 (MQ=255)

                                                                                                                                                           ttttgtttA‑TGT‑GCTTGAAGTTTTAATGCCCCGTCCTTG‑AGCAGCGGTAAAAATAATAAAAG‑CGTCG‑CCCTGAACACGAAATCAAATTTTA‑CAACATACTTCTCTACACGAATTTGGC‑TACAATCCAAAAAACAGAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:59752/234‑1 (MQ=255)

                                                                                                                                                           aCTTTTTTA‑TGA‑GAAGAAAGATTTAAGCCCTCCTCTATG‑AGCATGGGGAAAAAAAATGAATG‑CGTGT‑CCCTGGACACGAACAAAAAATTAC‑CAACACACTAACCTACTAGAATTTGCT‑TGGCTCCCACAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:521972/239‑1 (MQ=255)

                                                                                                                                                                       agaagatatgttttAATCCCTCCTCATTG‑AGCATCGGAAAAAATAAGAAATG‑CGTTT‑CCCTTGACACAAATAAAAAATTTC‑CAACACACTTACCTCCTCGATTTTGGC‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                 <  2:307948/228‑1 (MQ=255)

                                                                                                                                                                         gaaTGAAGTTTAAAGCCCCCCTCCTTG‑ATCACGGGGAAAATAAATGAAAG‑CGTGC‑CCTTTGCACCAAAAAAAAAATTTC‑CAACACCTTTATCTACCAGAATTTGGC‑TTGAATCAGAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                <  1:857306/236‑1 (MQ=255)

                                                                                                                                                                             gaaaattaaaccccccctccgTA‑ACCATGGGGAGAAATAATGAAAG‑CGGCC‑CTCTTGACAAGAATATAAATTCTC‑CAACCCCATTAGCTTCGCGAATTTCGC‑TTGCCCCCACAAAACCCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:230515/224‑1 (MQ=255)

                                                                                                                                                                             aaatattaatcccccccccctTG‑AGCAGCGGAAAAAAAAAAGAAGG‑C‑TGTCCCCTTGACAAGAAAAAAAAATTCA‑CAACACTTTTATCCACTCAATTTTGGC‑TTGCACCCG‑AAAAACCCGGTCCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:454699/221‑1 (MQ=255)

                                                                                                                                                                             aaagttttaacccccccactgtG‑AGCAAGGGGAAAAATAAAAAAAC‑CGTGC‑CCCTTGACACAAATAAAAATTTTC‑CAACACTCTTATCTACTCGATTTTGGC‑TTCCACCCGAAAAACCCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:741969/218‑1 (MQ=255)

                                                                                                                                                                             aaaGATTTAATGCCTCC‑CCTTGAAGCAGCGGGAAAAATAATGAACG‑CGTGT‑CCCTTGACAAAAATACAAATTTTA‑CAACCCATTTACTCACTCAATTTGGGC‑TCGCACCCACAAAACCAGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:764034/238‑1 (MQ=255)

                                                                                                                                                                                         cccccctcatttaagaTCGGGAAAAAATATGAAAA‑GGTTG‑TCCTTGACAAAAAAAAAAAATTTTCCACCACTTTTTTCAATTAGATTTTGTC‑TTCCACCAAAAAAAACAAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                 <  2:802651/224‑1 (MQ=255)

                                                                                                                                                                                           tcctcctTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                             >  1:487406/1‑143 (MQ=255)

                                                                                                                                                                                           tcctcctTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACCTAAAc                                       >  1:307948/1‑229 (MQ=255)

                                                                                                                                                                                           tcctcctTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAAAATTAAATAAACAAATTaattt                             >  2:358600/1‑235 (MQ=255)

                                                                                                                                                                                            cctcctTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                             >  1:556043/1‑158 (MQ=255)

                                                                                                                                                                                                cctgaagaagcggaaaaaaaaaTAAAAG‑CGTCT‑CCTTGCACAAAAATAAAAATTTTA‑CAACACTCTTATCTGCTAAATTTTGGC‑TGGCA‑CACAAAAAACCAGGTCCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttat                         <  1:437865/223‑1 (MQ=255)

                                                                                                                                                                                                                       gAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTa  >  1:234069/1‑239 (MQ=255)

AGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTA‑TGT‑GCATGAAGATTTAATGCCTCCTCCTTG‑AGCATCGGGAGAAATAATGAAAG‑CGTGT‑CCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTA  >  NC_000913/3665419‑3665869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A