Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,820,324 T→C 14.1% R282R (CGA→CGG)  ligB ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,820,3240TC14.1% 260.9 / 2.0 107R282R (CGA→CGG) ligBDNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base T (30/57);  new base C (18/2);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 6.69e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCA  >  NC_000913/3820088‑3820553
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
gtttaaggaccagcccccgccccgcgcCTGCGGGAACCAATATT‑CCCCCGCTGGACTTTTTTATCCTCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑CCCGCTT‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCC‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCg                                                                                                                                                                                                                                        <  2:565398/216‑1 (MQ=255)
gggcaaattcccccccccggCCAGGCCCTGAAGGAACAAAGTTT‑CACCCCCTGGACTTTTTTATCACCCACCATGCAAGGTGCAAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCCCTTCGGCACCCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCg                                                                                                                                                                                                                                        <  2:51189/220‑1 (MQ=255)
ggCCCAATTCCCCCCCCCTGCCAGGCCCCGCAGGAACCAATTTC‑CACCCCGTGGACTTTTTTATCATCCAGAATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTT‑TTACCCCCCGGAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCg                                                                                                                                                                                                                                        <  1:126755/239‑1 (MQ=255)
  cccaattccccACTCCTGCCAGCGCCTGACGGAACCAATTTT‑CCCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCg                                                                                                                                                                                                                                        <  1:626418/229‑1 (MQ=255)
      atggcccACTCCTGCAAAGGCCTGACGGAACCAATATT‑CCCCCGCTGGACTTTTTTAGCACCCAGCATGACAGGTGCGAGTGCCGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAAc                                                                                                                                                                                                                                  <  1:943530/235‑1 (MQ=255)
      atatCCCACTCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCCCCGCTCggaacac                                                                                                                                                                                                                                  >  1:248607/1‑233 (MQ=255)
       attCCCCCTCCCGCCCGCCCCTGACCGGAACAAGATTCCCCCCGGTGGCCTTTTTTACACTCCGCCCTGACAGGTGCGATGGACCCAACCCCCGTTTTTTT‑ACCCCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCACCCTGGGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAAc                                                                                                                                                                                                                                  <  2:819539/237‑1 (MQ=255)
            cACTCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTTGCCGCCCGTACAACTACGc                                                                                                                                                                                                                             >  1:51189/1‑238 (MQ=255)
            cACCCCTTCCAGCGCCTGACGGAACCAATATT‑CCCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGcc                                                                                                                                                                                                                            <  1:788005/239‑1 (MQ=255)
               tCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCacc                                                                                                                                                                                                                         >  1:465975/1‑237 (MQ=255)
               tCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCTTTCGCCCCTCGTCCAACTACCCCATc                                                                                                                                                                                                                         >  1:872668/1‑239 (MQ=255)
               tCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGCATTCTTGCCCTTTCCCCCCTCGTCCAACTCCGCCCTc                                                                                                                                                                                                                         >  1:58022/1‑239 (MQ=255)
                 cTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCCGGCCCTTTCCCCGCCCGTACAACTTCGCCCTCgg                                                                                                                                                                                                                       >  1:99444/1‑239 (MQ=255)
                   gCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCCCCTACAACTACGCCATCgggg                                                                                                                                                                                                                     >  1:275197/1‑237 (MQ=255)
                   gCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCTTTCGCCGCTCGTACAACTACGACATCGGTg                                                                                                                                                                                                                     >  1:787890/1‑239 (MQ=255)
                   gCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAAACACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGGGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCCGCTTTGCTCCCCGGACAAATACGCCATccgg                                                                                                                                                                                                                      >  1:793823/1‑235 (MQ=255)
                      ccccccagccGGAACTAATACT‑CCCCCGGTGGCTTTTTTTTCCCTCCGGCATGACAGGGGCGAGGGACCCAACCCCCGATTTTTT‑CCCCATA‑CTACCCCCCGCAAACTGAATGGCCTGCATTTCGCCAACCGGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACg                                                                                                                                                                                                                  <  2:283551/230‑1 (MQ=255)
                      aacgcCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCAGGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACg                                                                                                                                                                                                                  <  1:39030/237‑1 (MQ=255)
                        cgcCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCTTTTCCCCCCCGTACAACTACGcctgtcccttttac                                                                                                                                                                                                                >  1:565398/1‑227 (MQ=255)
                         gcCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCGCTTTCGCACATCGTTCAACTACGCCACCCGCGACCAAg                                                                                                                                                                                                               >  1:283551/1‑239 (MQ=255)
                                 ggcacctatttcccccccgtGGACTTTTTTACCACCCAGCATGACAGGGGGGGTGGACGCAACCACCGATATTTT‑ACCGCTG‑TTACCCACCGCAACCTGAATTGCCTTCACTTCGGCAACCTGAGCTGCAGGTTGATATTTCCAGGCCC‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAAtt                                                                                                                                                                                                        <  2:792209/220‑1 (MQ=255)
                                  aaCCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCCCCTTCCCCCCCCCTCCAACCACGCCCACGGGGCCTAAGGGGAATTc                                                                                                                                                                                                       >  2:786435/1‑238 (MQ=255)
                                   aCCAATATT‑CCCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTTCGAGTGACGCAACCACCGATATTTT‑ACCGCTT‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCg                                                                                                                                                                                                     <  1:342474/239‑1 (MQ=255)
                                     cAATTTT‑CCCCCGATGGTTTTTTTTACCCTCCAGCATGACGGGTGCGAGGTGCCCAACCCCCGATATTTC‑CCCGCGC‑TCACCCACCGCAAACTGAATGGCCTTCACTCCGGCAACCTGAGCTACGGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCt                                                                                                                                                                                                   <  2:85536/239‑1 (MQ=255)
                                         ttt‑CCCCCGCTGGATTTTTTTATCTTCCAGCCTGACGGGTGCGAGTAACCCAACCACCGATATTTT‑ACCGCTT‑TTACCCACCGCAAACTGAATGGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTcc                                                                                                                                                                                               <  2:419252/238‑1 (MQ=255)
                                           t‑CACCCGCTGGACTTTTTTACCATCCCGCATGACAGGTGCGAGTGACGCAACCCCCGATATTTT‑ACCGCTT‑TTACCCACCTCAAACTGAATGGCCTTCCCTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTccac                                                                                                                                                                                             <  1:710031/239‑1 (MQ=255)
                                            tcccccGCGTGACCTTTTTACCATCCACCATGACAGGTGCGAGGGCCGCAACCGCCGATCTTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTccac                                                                                                                                                                                             <  1:844002/236‑1 (MQ=255)
                                            ccccccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTccac                                                                                                                                                                                             <  1:310494/236‑1 (MQ=255)
                                             cACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCCCCCGTCCAACTACGCCACCGGCGCCGAAGGGGAATTCCCCttttccc                                                                                                                                                                                             >  1:749123/1‑234 (MQ=255)
                                              aCCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGGGACGACGGGTAATTCCGCTTTccaca                                                                                                                                                                                            >  1:226197/1‑237 (MQ=255)
                                                ccGCTGGCCTTTTTTATCATCCCGCATGACAGGGGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTCCCCCCCGCAAACTAAAGTGCCTTCCCTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCC‑CCCGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACt                                                                                                                                                                                         <  2:805083/239‑1 (MQ=255)
                                                ccGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGAGTCTGGCTCTTTCCACGCCCCTCCAACTACCCCACCCGGGACAAAGGGGaaaataccgtttccccacc                                                                                                                                                                                         >  2:528009/1‑222 (MQ=255)
                                                 cGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGTTTCTGGCCCTTTCGCCGCCCGTACAAACACGCCATCGGTGAAGAAGGGTGAATTCGCTTTCcaccaccc                                                                                                                                                                                        >  2:516926/1‑237 (MQ=255)
                                                   cTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTTTGGCTCTTTCCCCGCCCGTAAAACTACGCCATCGGGGCAGAAGGGGAATTCCGCTTTCccccacccct                                                                                                                                                                                      >  1:69759/1‑235 (MQ=255)
                                                   cTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTTTCGCTCTTTCGCCGCCCGTACAACTACCCCATCCGGGCAGAAGGGGAATTACCCTTTCCCCCCCTCAt                                                                                                                                                                                      >  1:1045684/1‑239 (MQ=255)
                                                   cTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCCCTTTCGCCCCCCCTACACCTCCCCCCACGGGGTGGACGGGGAAATCCCCCTTCCCCCACCCAt                                                                                                                                                                                      >  2:893667/1‑239 (MQ=255)
                                                                   tcCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCCCTTTTGCCGCCCGTACAACTACCCCCTCGGGGACGAAAGGGAATTCCCCTTTCCACCCCCCAATGCG‑C‑ACGCGCGCAAc                                                                                                                                                                    >  2:530972/1‑239 (MQ=255)
                                                                     cAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTTTGGCCCTTTTGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGGAATTCCCCTTTCCACAACTCATTGCG‑T‑ACGCGCCCACCt                                                                                                                                                                   >  1:696494/1‑238 (MQ=255)
                                                                         aTGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTtcat                                                                                                                                                               <  1:786435/238‑1 (MQ=255)
                                                                                gTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGCAGCCAATGGCGGGGTTCTGGCCGCTTCCGCGCCCGTACCACCACCCCCTCCGGGACGACGGGTAATTCCCCTTTCCACCACTCATTTCG‑C‑ACGCGCGCCACCtcacgaacaatt                                                                                                                                                       >  2:257629/1‑230 (MQ=255)
                                                                                        tgccacccccccgctttttc‑ACCGTTC‑TTACCCCCCGCAAACTGAATTGCCTTCATTTCCCAACCCTAAGCTACTGGGTGATATTTACCGGCCA‑CCAGACACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGc                                                                                                                                               <  2:821823/223‑1 (MQ=255)
                                                                                        gACGCACCCCCCTATTTTTT‑CCCGCTC‑TTCCCCCCCGCAACCTGAATTCCCTTCCCTCCGGCAACCGGGGCTACAGGTTGATATTCCCAGGCCC‑CCAGCCACCCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCg                                                                                                                                                <  2:832932/238‑1 (MQ=255)
                                                                                        gACGCAACCACCGATATTTT‑CCCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCg                                                                                                                                                <  1:573533/238‑1 (MQ=255)
                                                                                          ttaagagacatttatTTT‑ACCCCCT‑TTACCCCCCGAAAACCGAATTGCCTTCACTGCGGCCACCGGGCCTACAGGTTGATTTTTCCGGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                             <  2:811820/227‑1 (MQ=255)
                                                                                          cgcccccccctgtatTTT‑TCCCCTCTTTCCCCCCCGAAAACCTAATGCCCTTCCCTTCGGCCTCCGGGGCTTCGGTTTGTTTTTCCCGGGCCA‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCCCCGCa                                                                                                                                              <  2:887731/227‑1 (MQ=255)
                                                                                          cGCAACCCCCGATATTTT‑CCCGCTC‑TTACCCTCCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTTATATTTCCAGGCCA‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                             <  1:49932/239‑1 (MQ=255)
                                                                                           cccaccctccctttttttccccttc‑tTCCCCCCCCAAACCTGAATTCCCTTCCCTTCGGCAACCTGGGCCACAGGTTGATTTTTCCAGGCCC‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                             <  2:55705/216‑1 (MQ=255)
                                                                                                ctcctttttttt‑CCCTCTT‑TTACCCACCCTAAACGGAATGGCCTTCACTTCGGCAACCTGAGCTACGGTTTGATATTCCCAGGCCC‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGtat                                                                                                                                       <  2:271088/231‑1 (MQ=255)
                                                                                                caccGATTTTTTCCCCGCT‑‑TTACCCACCGCAAACTGAATTGCCTTCCCTTCGGCAACCTGAGCGACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGtat                                                                                                                                       <  1:1054155/239‑1 (MQ=255)
                                                                                                caccGATATTTT‑CCCGGCT‑TTTCCCCCCGCAAACGGAATGGCCTTCCCTTCGGCAACCGGAGCTAAAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGtat                                                                                                                                       <  1:575336/239‑1 (MQ=255)
                                                                                                    gATATTTT‑ACCGCTC‑TTACCCCCCGCAAACTGAATTGCCTTCCCTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑Gt                                                                                                                                  <  1:393307/239‑1 (MQ=255)
                                                                                                        tttt‑ACCGCTC‑TTACCCCCCGCAAACTGAATTGCCTTCCCTGCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCAc                                                                                                                                             <  1:816299/225‑1 (MQ=255)
                                                                                                         ttt‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCCCCGCCCCTACAACTACACCATCGGTGACGCAGGGGAATTCACCTTTCCCCCACCCCTTCCC‑TCACGC‑CGCAAATTCATCCACActttttcccccccgggaaaaagtgtgcg                                                                                                                              >  2:578698/1‑216 (MQ=255)
                                                                                                           ttcccGCCC‑TTCCCCCCCCAAAACTGAATTGCCTTTCCTTCGGCAACCTGGGCTACAGGTTGATTTTTCCAGGCCA‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGt                                                                                                                            <  2:213028/236‑1 (MQ=255)
                                                                                                            ttcccctttttACCCCCCCCAAACTGAATGGCCTTCACCCCGGCACCCGGGGCTACAGGTTGATATTCCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGt                                                                                                                            <  2:1066285/230‑1 (MQ=255)
                                                                                                            ccccGCTC‑TTCCCCCCCGCAAACTGAATTTCTGTCCCTCCGCCACCCTGGGCTCCAGGTTAATTTTTCCAGGCCC‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGtt                                                                                                                           <  2:279933/237‑1 (MQ=255)
                                                                                                              ccGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCCATGGCGGGATTCTGGCTCATTCGCCGCTCGTACCACTACGCCATCGGTGACGACGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCACGCAACTTCATCAGCATTTTTCACCCCACCGGTATAC‑GTCTCCGTTaa                                                                                                                         >  1:281226/1‑238 (MQ=255)
                                                                                                                 ctc‑tTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCCCTCATTGCG‑T‑ACGCCCGCAACTTCATCAGCATTTTTCACCGCACGGGGATAC‑GTCTGCGTTAGAGt                                                                                                                      >  1:213028/1‑239 (MQ=255)
                                                                                                                 ctc‑tTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCCTTTCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTTCCCGCACGGGGTTAC‑Gt                                                                                                                                  >  1:811820/1‑227 (MQ=255)
                                                                                                                 ctc‑tTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGGGCAACTTCATAAGCATTTTTCACCGCACGGGTTTAC‑TGCTGGGTTTGAGt                                                                                                                      >  1:831317/1‑239 (MQ=255)
                                                                                                                   c‑tTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGGTTCTGGCTCTTTTCCCGCTCGTCCAACTAACCCATCGGGGACGAAGGGTAAATCCGCTTTTCACCACTCTTTCCGCT‑TCGCGC‑CCACTCCTCCACCATTTTTCACCCCACGGGGATAccgcctgttccttaaaa                                                                                                                     >  2:816299/1‑223 (MQ=255)
                                                                                                                       aCCCCCCGGAAACTGAATTGCCTTCCCTTCGGCAACCTGGGCTGAAGGTTGATTTTCCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGTTTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAAc                                                                                                                 <  1:516926/239‑1 (MQ=255)
                                                                                                                            ccGCAAACTGAATTGCCTTCACTGCGGCCACCTGAGCTACAGGTTTATATTTCCAGGCCC‑CCAGCCCCTCTGCCTGGGCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCg                                                                                                            <  1:1037582/239‑1 (MQ=255)
                                                                                                                             cGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTccacca                                                                                                                                                                                           <  1:853766/162‑1 (MQ=255)
                                                                                                                             cGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCCTTTTTCACCGCCCGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCgg                                                                                                           >  1:298205/1‑239 (MQ=255)
                                                                                                                             cGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCCCACCGGTATAC‑GCCTGCGTTAGAGTAAAAccccg                                                                                                             >  1:279933/1‑234 (MQ=255)
                                                                                                                                    tGATTTCCCTTCTCCTCGCAACCCTGGGCTCCAGGTTGATATTCCCAGCCCC‑CCAGCCCCCCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAg                                                                                                    <  2:465975/239‑1 (MQ=255)
                                                                                                                                      aaTTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGGGACGAAAGGCAATTCCGCCTTCCCCCACTCATTGCG‑T‑ACCCCCGCAACTTCATCAGGCTTTTTCACCCCACGGGGATACGGTCCG‑GTTAAAGGAAAACCTCCGGTAACCacc                                                                                                   >  2:10749/1‑236 (MQ=255)
                                                                                                                                            cTTCACTTCGGCAACCGGAGCTTCAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTa                                                                                            <  1:180432/239‑1 (MQ=255)
                                                                                                                                              tcctctcggcacccgaggCTACAGGTTGTTATTTCCAGCCCC‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTaaa                                                                                          <  2:248607/222‑1 (MQ=255)
                                                                                                                                                  ttCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCCCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGAt                                                                                      <  1:923690/239‑1 (MQ=255)
                                                                                                                                                  ttCGGCAACCTGAGCTACAGGTTGATATGTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGAt                                                                                      <  1:530972/239‑1 (MQ=255)
                                                                                                                                                  tgggcgacctcgggttACGGGTTG‑TATTTTCACGCCCCCCAGCCACCCGGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGAt                                                                                      <  2:69759/224‑1 (MQ=255)
                                                                                                                                                  tccgccacccTGGGCGACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGAt                                                                                      <  1:893667/231‑1 (MQ=255)
                                                                                                                                                          ccTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCCCGTACAACTACGCCATCGGGGACGAAGGGTAATTCCCCTTTCCACCACTCATTGCG‑G‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCCCCGGTAACC‑CACTGCGTTGAAGAAAAAACCGCGGTAGCCCGCCCCTTTaaaacacacaaaaaa                                                                              >  2:257735/1‑227 (MQ=255)
                                                                                                                                                                       ttGATATGTCCAGGCCA‑CCGGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGt                                                                     <  1:302670/235‑1 (MQ=255)
                                                                                                                                                                        ttgattttccgcccccccctgCCCCTCTGCCGGGCCCCGAGGTCAGGGGCGGGGTTCCGTCTCTTTCGCCGCCCGTACCACGACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCAt                                                                 <  2:159614/219‑1 (MQ=255)
                                                                                                                                                                        ttatttttGCATGCCC‑CCCGCCACTCTGCCGGGCCCGGTAGCCAATGGCGGAATTCTGGCTCTTTCGCCGCTCGTACAACTCCGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATc                                                                <  2:226197/234‑1 (MQ=255)
                                                                                                                                                                                    gCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAAATACCCCATCGGGGACGAAGGGTAATTTCGCTTTTCACCACTCATTGCG‑G‑ACGCGCGCCACTTCATCAGCATTTTTTACCGCACCGGGATAC‑GTCCTCGTTAGGGTAAAAACTCCGGTAGCCCGCCCTTTTAAACGATCCGACCTTAAcaccgcccccccccgcccagcccc                                                    >  2:808225/1‑216 (MQ=255)
                                                                                                                                                                                      ca‑ccaGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCAc                                                                                                                                                  <  1:428624/144‑1 (MQ=255)
                                                                                                                                                                                      ca‑ccaGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTaa                                                                                           <  1:272939/198‑1 (MQ=255)
                                                                                                                                                                                          caGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTTGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGGTCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGCCaa                                               >  1:184059/1‑239 (MQ=255)
                                                                                                                                                                                               aCTCTGCCTGGCCCGGTAGCCAATGGCGGGATTTCCGCTCTTTCGCCGCTTGTTCAACTACGCCCTCGGTTACGAAGGGTAATTCCGCCTTTCACCCCTCATTGCG‑T‑ACCCCAGCAACTTCATCAGCATTTTTTCCCGCCCGGGTATAC‑GCCTGAGTTTGAGTACAACCTTCGGCTGCCCGCTCTTTTTAACCACTCTAAATTAACCCCGGTCCATCCCGCCAATCCCCAACaaaaaa                                           >  2:315868/1‑237 (MQ=255)
                                                                                                                                                                                                 tctGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAAAGc                                         >  1:841918/1‑238 (MQ=255)
                                                                                                                                                                                                 tctGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGGAATTCCGCTTTCCACCACTCATTTCG‑T‑ACGCGCGCAACTTCTTCAGCATTTTTTACCGCACgggga                                                                                                                                        >  2:428624/1‑142 (MQ=255)
                                                                                                                                                                                                  ctGCCTGGCCCGGTTGCCAATGGCGGGATTCTGGCTCTTTCGCCGGTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGccc                                       <  1:409887/239‑1 (MQ=255)
                                                                                                                                                                                                   tGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACt                                                                                                                                                                                         <  1:855410/95‑1 (MQ=255)
                                                                                                                                                                                                   tGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACt                                                                                                                                                                                         >  2:855410/1‑95 (MQ=255)
                                                                                                                                                                                                    gCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCag                                     <  1:10749/239‑1 (MQ=255)
                                                                                                                                                                                                       tGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCACACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTTCGGGCCATCCGGCCAAGCCCCGACAAAAACGCCCagaaa                                  >  1:844064/1‑237 (MQ=255)
                                                                                                                                                                                                          cccGGTAGCCAATGGCGGTGTTCTGGCTCTTTCGCCCCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑G‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAAtt                               <  2:555698/239‑1 (MQ=255)
                                                                                                                                                                                                          cccGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCCTCGGTGACGAAGGGTAATTCCGCTTTCCACCCCTCATTTCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCCCGGGGATAC‑GTCTGCGTTAGAGTAAAACCTCAGGTTGCCCGATACTTTAAAAGATCAGACATTTAATGCGGTCCATCCGGCCCTTCCCCGACAAAAACACCCCAAGAAt                                >  2:632249/1‑238 (MQ=255)
                                                                                                                                                                                                                     aTGGGGGGTTTTTTGCTTTTTCGCCCCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCCCCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGc                    <  2:1001188/239‑1 (MQ=255)
                                                                                                                                                                                                                      tGGCGGTATTTTTGTTCTTTCGCCCCTCGTACACCTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCt                   <  2:298205/239‑1 (MQ=255)
                                                                                                                                                                                                                        gggggttttcggCTTTTTCCCCGCCCGTAACACTACGCCATCGGTGACGAAGGGTAATTCCGGTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTa                  <  2:841918/228‑1 (MQ=255)
                                                                                                                                                                                                                           ggATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGGCATTAACTGCGGTCCATCCGGCCATGCCCAGCCAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCg               >  1:44348/1‑238 (MQ=255)
                                                                                                                                                                                                                                        ttCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGcatca  >  1:861740/1‑238 (MQ=255)
                                                                                                                                                                                                                                         tCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCa                                      >  2:284166/1‑201 (MQ=255)
                                                                                                                                                                                                                                               cTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCg                                                                                                                                                                                                     <  2:716539/39‑1 (MQ=255)
                                                                                                                                                                                                                                               cTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCg                                                                                                                                                                                                     >  1:716539/1‑39 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
GGCGCAATATCCCACTCCTGCCAGCGCCTGACGGAACCAATATT‑CACCCGCTGGACTTTTTTATCATCCAGCATGACAGGTGCGAGTGACGCAACCACCGATATTTT‑ACCGCTC‑TTACCCACCGCAAACTGAATTGCCTTCACTTCGGCAACCTGAGCTACAGGTTGATATTTCCAGGCCA‑CCAGCCACTCTGCCTGGCCCGGTAGCCAATGGCGGGATTCTGGCTCTTTCGCCGCTCGTACAACTACGCCATCGGTGACGAAGGGTAATTCCGCTTTCCACCACTCATTGCG‑T‑ACGCGCGCAACTTCATCAGCATTTTTCACCGCACGGGTATAC‑GTCTGCGTTAGAGTAAAACCTGCGGTAGCCAGCTCTTTTAAACGATCAGACATTAACTGCGGTCCATCCGGCCATGCCCAGACAAAAACGCCCAGAGAATTCAGCGTGTCGCTATCGTCCTGGCGCATCA  >  NC_000913/3820088‑3820553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A