Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F17 I0 R1 124 155.2 3079696 98.3% 3027341 226.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,675,488 T→G 17.7% V235G (GTG→GGG)  yhjE → putative MFS transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,675,4880TG17.7% 369.5 / 12.4 139V235G (GTG→GGG) yhjEputative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/76);  new base G (26/2);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 1.08e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CCGCGCAAACGTGCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCGCTGGATTAC  >  NC_000913/3675250‑3675715
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   
ccGCGCAAACGTTCAGTGTCTGTCTCCGTTCGGCAGCTGTGCGCACCGTTTGTCTTCTTCTTTGCCAATGGCACTGTCGTGCTGTTTTCCTTGCTGCTAACGGGCGAGCGGTTTATGAGCTGGGGCTGGCGTGTGCCATTTGTCTTGCCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGGCGCC‑GGTG‑TT‑T‑GAGAAAGGCGCTAAAGC‑GAAAAAACAGGt                                                                                                                                                                                                                                     <  2:530367/239‑1 (MQ=255)
     cAAACGTGCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGGGCTGGGGCTTATCCGGGCGTTAG‑TTTGCGTT‑TGG‑CTGCC‑GTAGGTGTC‑CGGG‑TT‑T‑GAGAAACGTGCCATAGC‑GAAAAAACAAGCGAAGa                                                                                                                                                                                                                                >  2:466952/1‑239 (MQ=255)
        aCGTGCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCC‑TGAGTTGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GCAAAAAAAGGGGAAGATc                                                                                                                                                                                                                              >  1:1094559/1‑238 (MQ=255)
            ggaCTTTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTGCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCt                                                                                                                                                                                                                         <  2:775792/237‑1 (MQ=255)
            gCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGGTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCt                                                                                                                                                                                                                         <  1:1469475/239‑1 (MQ=255)
            gCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCt                                                                                                                                                                                                                         <  1:63890/239‑1 (MQ=255)
                tGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTTCTCGTCATTTTCGGCCTGTATG‑TTTTCGGG‑TCG‑CCTCC‑TTAGTCTGC‑CGTG‑TT‑T‑TTGAAAGTCGCTCAAGC‑GGAAAAACCGGGGGAGATCCAGcccggt                                                                                                                                                                                                                     >  2:63890/1‑234 (MQ=255)
                 gTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTa                                                                                                                                                                                                                    <  1:827672/239‑1 (MQ=255)
                 gTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTa                                                                                                                                                                                                                    <  2:274646/239‑1 (MQ=255)
                  tATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTTGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTAc                                                                                                                                                                                                                   >  1:425839/1‑239 (MQ=255)
                  tATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGGGCCGGGCCTTATTGGCCTTTATG‑TTTGGCGG‑TGGCCGGCT‑TGGGTGGCC‑‑GTG‑TT‑T‑GGGAAAGGCCCTAAAGA‑GGAAAAACAAGGGGAGAAACCGCTgggggc                                                                                                                                                                                                                   >  2:820484/1‑236 (MQ=255)
                    tGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCCGGATGTTTGGCGGT‑TGC‑CTGC‑‑TGGGTGGCCGGGGG‑TT‑T‑GGGAAAGT‑GGTAAAGCGGAAAAAACAGGGGAAGAACCC‑CTggggaggc                                                                                                                                                                                                                 >  2:338936/1‑234 (MQ=255)
                       cTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGGGGTGGTCATTATTGGCCTGGTTT‑TTCTCGTGTTCG‑C‑GCATTGAGCGGCC‑GGTG‑TTGT‑GA‑AAATTCCCTAAACG‑GAAAAACCAGGGGagattcccctcgggtacccg                                                                                                                                                                                                                >  2:256252/1‑219 (MQ=255)
                          cTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGa                                                                                                                                                                                                           <  1:820484/239‑1 (MQ=255)
                          cTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGATGGGCATTTTCGGCCTGTTTG‑TTTGCGTG‑TTG‑CTGCC‑TGAGTAGCC‑GGTG‑GT‑T‑GGTGAAGTCGGTAAAGG‑AGAAAAACAGGGGAAGAACCCGCTGGGGACgttgccg                                                                                                                                                                                                            >  2:1121240/1‑236 (MQ=255)
                            ttCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTTGTGGGTCTTATCGGCCTGTATG‑GTCGCGTG‑TCG‑GTTGA‑TGAGTAGCC‑GGGG‑GT‑T‑TGGAAAGTTGGCAAAAC‑GAGAAAACAGGGTAAGGTCCCGCGCGGTACGGTGCTGGcc                                                                                                                                                                                                         >  2:771901/1‑239 (MQ=255)
                            ttCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTTTCCGCCTGTATG‑TTTGCGTG‑TCT‑CTGCA‑TGAGGCGCC‑CGGG‑TT‑T‑TTGAAAGTCGCTAAAGC‑GGAAAAACAGGCGTAGATCCCGCTTGGTACGACGCTGag                                                                                                                                                                                                          >  1:45955/1‑237 (MQ=255)
                            ttCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGGCATTATCGGCCTGTATG‑TTCGCGGG‑GCG‑CTGCA‑TTAGGCGCC‑CGGG‑TT‑T‑GTGTAAAACGCTAAAGC‑GAAAAAAAAGGGGAAGATCCCGCCTGGTAGgccgcttgc                                                                                                                                                                                                          >  2:1161449/1‑235 (MQ=255)
                            ttCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCCGGTCCTTATCGGCCCGGATG‑TTCCCGTG‑TTG‑CTTCC‑TGCGGCGCC‑GGGGTTT‑T‑GTGAAAG‑CGCTTAAGC‑GAAAAAAAAAGGTAATATACCGCCTGGGACGCCGCTGAc                                                                                                                                                                                                          >  2:394530/1‑238 (MQ=255)
                            ttCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGGGCTGGGCATTTTCGGCCTGTATG‑TTTGCGGG‑TTG‑CTGCA‑TGAGTCGCC‑CGGG‑TT‑TGGGGAAAGT‑GCTTAAGG‑GGAAAAACAGGGGGAGAACCCGCCGGGGACGccgctggc                                                                                                                                                                                                          >  1:833213/1‑236 (MQ=255)
                                 cAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTTGTTATTATCGGGCTGTATG‑TTCGCGCG‑TTC‑CTGCA‑TGAGTTGCA‑CGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAGGCACCCGCTGGGTACGTTGCTCACCAAACa                                                                                                                                                                                                    >  1:1023029/1‑239 (MQ=255)
                                    cTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTTTCCGCCTTTTTG‑TTTTCGTG‑TCG‑CTGCT‑TGGGTCCGC‑GGGG‑TT‑T‑TGGAAAGTCGCTCAAGC‑GAAAAAACAGGGGGAGATACCCCCGGGTCAGCCGCTGACCCAAAATGt                                                                                                                                                                                                 >  2:192632/1‑239 (MQ=255)
                                     tGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACAt                                                                                                                                                                                                   <  2:50055/236‑1 (MQ=255)
                                        gcgcACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTcg                                                                                                                                                                                              <  1:1443874/238‑1 (MQ=255)
                                          gcACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTcgcg                                                                                                                                                                                            <  2:1094091/238‑1 (MQ=255)
                                            gccGATTTGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TGCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTaa                                                                                                                                                                                         <  2:1350923/238‑1 (MQ=255)
                                            aCCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTaa                                                                                                                                                                                         <  1:1170184/239‑1 (MQ=255)
                                            aCCGATTGGCTTCTTCTTTGCCAATGGCACTGTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTTTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTaa                                                                                                                                                                                         <  1:1185233/239‑1 (MQ=255)
                                            aCCGATTGGCTTCTTCTTCGGCAATGGCACTTTCTTGCTGCTTTCCTGGCTGCTGACCGACGAGGAGTTTATGAGCTGCGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTTGTCATTATCGGCCTGTATG‑TTCGCATG‑TCG‑CTGCA‑TGATGCGCC‑GGTC‑TT‑T‑GAGAAAGACGCTAAAGC‑GAAACAACAGGTGACGATCCCGCTTGTTACTCTGCTGACCAAACATGTTGGCTTaa                                                                                                                                                                                         <  2:405175/239‑1 (MQ=255)
                                             cctaTTTGCTTCTTCTTTGCCAATGGCTCTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAAc                                                                                                                                                                                        <  2:838241/236‑1 (MQ=255)
                                                 ttGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTa                                                                                                                                                                                    <  2:878446/239‑1 (MQ=255)
                                                 ttGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTa                                                                                                                                                                                    <  2:721953/239‑1 (MQ=255)
                                                 ttGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTAGCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTa                                                                                                                                                                                    <  1:875362/239‑1 (MQ=255)
                                                  tGGCTTCTTCATTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTAc                                                                                                                                                                                   <  1:544669/239‑1 (MQ=255)
                                                        cttcttTGCCAATGGCACGTTCTTGCTGCTTTCCTGGCTTCTGACCGACAAGCAGTTTATGAGCTGGGGCTGGCGTTTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGGC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTa                                                                                                                                                                             <  2:183827/239‑1 (MQ=255)
                                                         ttcttTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTAc                                                                                                                                                                            <  1:227060/239‑1 (MQ=255)
                                                                        ccTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGc                                                                                                                                                             <  2:896170/238‑1 (MQ=255)
                                                                        aCTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGc                                                                                                                                                             <  1:654138/239‑1 (MQ=255)
                                                                        aCTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGc                                                                                                                                                             <  1:287517/239‑1 (MQ=255)
                                                                                  tgctTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACg                                                                                                                                                   <  1:477614/239‑1 (MQ=255)
                                                                                  tgctTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACg                                                                                                                                                   <  1:466952/239‑1 (MQ=255)
                                                                                  tgctTTCCGGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACg                                                                                                                                                   <  2:1266551/239‑1 (MQ=255)
                                                                                  tgcgTTCCTGGCTACGTACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACg                                                                                                                                                   <  2:667178/239‑1 (MQ=255)
                                                                                   gctTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGc                                                                                                                                                  <  2:1063173/239‑1 (MQ=255)
                                                                                                 tGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATg                                                                                                                                    <  1:1481150/239‑1 (MQ=255)
                                                                                                 tGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGCTTTACATCATg                                                                                                                                    <  2:369831/239‑1 (MQ=255)
                                                                                                 tGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGGCATTTTCGGCCTGGTTG‑TTCGCGGG‑TCG‑CTGCA‑TTGGTCGCC‑GGGG‑TT‑T‑GTGGAAGACGCTCAAAC‑GGAAAAACAGGGGGAAATCCCGGTGGGGTCACGCGTGACCAAAACTGGTTGCGTAACCGGACTGGGTACGGTCATTATAGGGGGCACCTATAAGCCGTTTTTcatcatt                                                                                                                                    >  2:832703/1‑238 (MQ=255)
                                                                                                      gacgaGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGt                                                                                                                               <  2:425839/239‑1 (MQ=255)
                                                                                                        cgaGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCCTTATCGGCCTGGGTG‑TTTGCGTG‑TCT‑CTGCA‑TGAGTCGCC‑GGGG‑TT‑T‑GAGAAAGTCGCCAAAGC‑GAAAAAAAAAGGGAAGATCCCGCTGGGTACGCTGCTCGCCAAACATGTTCGCGTAAACCGACAGGGGTCGGTCTTTTTTCTTGCAACCTATACGCCGTTTTTTATCATGAcaggc                                                                                                                              >  2:1040157/1‑234 (MQ=255)
                                                                                                        cgaGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTc                                                                                                                              <  1:217503/238‑1 (MQ=255)
                                                                                                        cgaGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCt                                                                                                                             <  1:338936/239‑1 (MQ=255)
                                                                                                         gaGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTa                                                                                                                            <  1:689158/239‑1 (MQ=255)
                                                                                                                ttATGAGCTGGGTCTGGCGGGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATg                                                                                                                     <  2:614851/239‑1 (MQ=255)
                                                                                                                 tATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGGGATTTTCGGGCGGGATG‑TTCGGGTG‑TCC‑GTGGA‑TGAGTGGCC‑GGGG‑TT‑T‑GGGAAAATCGCTAAAGA‑GAAAAAACAGGGGAAGAACCCGCATGGGAAGGTGCTGGCCAAAAAAGTTTGCGGTACCGTACTGGGGACGGTCCATATGCGTGCCACCTATACCCTGTTTTTAATCATGGCgggtttctccatgt                                                                                                                    >  2:1119973/1‑227 (MQ=255)
                                                                                                                         ggggCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGGCATTTTCTGGCTGTATG‑TTTGCGTG‑TCG‑CTGGA‑TGAGGCGCC‑GGGG‑TT‑T‑TAGAAAAGCGCTAAAGC‑GGAAAAAAAGGGGGAGATCCCGCTGGGGACGCCGCCGACCCAACATTTTCGCGCAACCGCTCTGGGGACGGTTCTTTTTCTGGCAAACTCTTCGCCGGTTTTCATCATGACGGTCTAcctcttgacccttttt                                                                                                            >  2:117941/1‑224 (MQ=255)
                                                                                                                         ggggCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGGCATTATTGGCCTGTTTG‑TTTTGGTG‑TTG‑CCGCA‑TGAAGCGCC‑GGGG‑GT‑T‑TGGAAAGTCGCTTCATC‑GGAAAAACAAGGGAAGATCCCGCCGGGTACCCCGCTGACCAAACATGTTCGCGGAAACGTa                                                                                                                                                                                    >  2:218826/1‑167 (MQ=255)
                                                                                                                           ggCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGc                                                                                                                                                  <  2:1343695/199‑1 (MQ=255)
                                                                                                                                gCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACg                                                                                                                                                   <  1:474235/193‑1 (MQ=255)
                                                                                                                                    gggCCATTTATTTTCGCGGCGGTGGTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACcgccgccgc                                                                                                 <  2:158148/237‑1 (MQ=255)
                                                                                                                                     tgCCATTTATCTTCTCGGCGGTGCTGGGCATTTTCGGCCTGTATG‑TTTGCGTG‑TCG‑CTGCA‑TTAGTCGCC‑GGTG‑TT‑T‑TAGAAAGTCGCTAAAGC‑GAAAAAACAGGGGAAAATCCCGCTGGGGAAGCTGCTGACCCAACATGTTTGCGTAACCGTACTGGGGTCGGTCATTATGCTGGCAACCTATACGCTGTTTTAcat                                                                                                                                        >  1:1343695/1‑199 (MQ=255)
                                                                                                                                          tttATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGt                                                                                           <  1:192632/239‑1 (MQ=255)
                                                                                                                                          tttATCTTCGCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGt                                                                                           <  1:1492227/239‑1 (MQ=255)
                                                                                                                                               tagaagacaggTGCTGGTCATTATCGGCCTGAATG‑TTCGCTTG‑TCG‑CTGCA‑TTAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGGGAAGATCCCGCTGGTTACGCTGCTGACCAAACATGTTCTCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGc                                                                                      <  2:1244527/230‑1 (MQ=255)
                                                                                                                                               cttctCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGc                                                                                      <  1:542449/239‑1 (MQ=255)
                                                                                                                                                  ctcGGCGGTGCTGGTCATTTTCGGCCTGTATG‑TTTGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGGG‑TT‑T‑TGGAAAGTCGCTTAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCCGCTGACCCAAAATGTTCGCGCAAACGCGCTGGGGACGTTCCTTTTGCCGGCAACCTATACGCTGTTTTTCATCATGAGAGTCTATCTTTTTACCTTTTGCTCCCCCCCCCGGCCACTTGGGCttt                                                                                   >  2:496322/1‑238 (MQ=255)
                                                                                                                                                             tGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGGCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCcgcg                                                                         <  2:865724/238‑1 (MQ=255)
                                                                                                                                                               gTCCTTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CCGCC‑TGAGTCGCC‑GGGG‑TT‑T‑TAGAAAATCGCTAAAGC‑GGAAAAACAGGGGAAGATCCCGCCGGGTACGCCGCTGACCAAACATGTTCGCGTAAACGTACTGGGGACGTTTATTATGCTGGCAACCTATACGCTGTTTTTCATCATGACGGTCTTCTCTATGAGCTTTAATACCGCCGCCCCCCCCAGTGGGCCTGGCCTGCCCCGTa                                                                       >  2:666465/1‑238 (MQ=255)
                                                                                                                                                                   ttATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGaa                                                                  <  1:1040157/239‑1 (MQ=255)
                                                                                                                                                                   ttATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGaa                                                                  <  1:265727/239‑1 (MQ=255)
                                                                                                                                                                   ttATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑GT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGaa                                                                  <  1:240625/239‑1 (MQ=255)
                                                                                                                                                                    tATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTACCGAAg                                                                 >  2:1417645/1‑239 (MQ=255)
                                                                                                                                                                    tATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAg                                                                 >  2:748882/1‑239 (MQ=255)
                                                                                                                                                                    tATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAg                                                                 >  1:810594/1‑239 (MQ=255)
                                                                                                                                                                    tATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCCGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGAGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAg                                                                 >  2:743061/1‑239 (MQ=255)
                                                                                                                                                                        ggCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGtgtt                                                             <  1:660523/239‑1 (MQ=255)
                                                                                                                                                                         gCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGtgttg                                                            >  1:1211038/1‑239 (MQ=255)
                                                                                                                                                                         gCCTGTATG‑TTCGCGCG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTAATTATGCTGGCAACTTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGTGCCAGTTGGGCTTGGCCTGTCGCGTAACGAAGtgttg                                                            >  2:1306511/1‑239 (MQ=255)
                                                                                                                                                                              tATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGAt                                                       >  2:1432528/1‑239 (MQ=255)
                                                                                                                                                                              tATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGAt                                                       >  1:219897/1‑239 (MQ=255)
                                                                                                                                                                                 g‑TTCGCTTG‑TCG‑CTGCA‑TGAGTCGGC‑GGTG‑TT‑T‑TAGAAAGTCGATAAAGC‑GAAAAAACAGGTGAAGACCCCGCTGGGTACGCTTCTGACCAACCATGTTCGCTTAACCTTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTTTTTTACATCATGACGGTCTACTCTATGACCTTTAGTGCCGCCTCCGCGCCAGTTGGGCTTGGTCTGCCGCGTAACGAAGTGTTGTGGATGtt                                                    <  2:460027/239‑1 (MQ=255)
                                                                                                                                                                                     cgcgTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTtgat                                                 >  1:1088144/1‑239 (MQ=255)
                                                                                                                                                                                     cgcgTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTtgat                                                 >  1:1503424/1‑239 (MQ=255)
                                                                                                                                                                                         tg‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTc                                                                                                                              >  2:1114403/1‑158 (MQ=255)
                                                                                                                                                                                         tg‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTc                                                                                                                              <  1:1114403/158‑1 (MQ=255)
                                                                                                                                                                                         tg‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGatgatgatgatg                                             >  2:86591/1‑239 (MQ=255)
                                                                                                                                                                                         tg‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGatgatg                                             >  1:346076/1‑239 (MQ=255)
                                                                                                                                                                                         tg‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGatgatg                                             >  2:700941/1‑239 (MQ=255)
                                                                                                                                                                                          g‑tCG‑CTGCA‑TGAGTCGCC‑GGTT‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATgg                                            <  1:704118/239‑1 (MQ=255)
                                                                                                                                                                                          g‑tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATgg                                            <  1:1480005/239‑1 (MQ=255)
                                                                                                                                                                                          g‑tCG‑CGGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGatgatg                                             <  2:109013/238‑1 (MQ=255)
                                                                                                                                                                                            tCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGc                                           >  1:1144903/1‑239 (MQ=255)
                                                                                                                                                                                                              c‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCGTTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCCCGTCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATTGCAGTTTTTGGTTTTgtc                           >  1:1508933/1‑237 (MQ=255)
                                                                                                                                                                                                              c‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGc                           >  2:1061513/1‑239 (MQ=255)
                                                                                                                                                                                                                        t‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATgg                    >  2:1384488/1‑239 (MQ=255)
                                                                                                                                                                                                                               aGTCGCTAAAGT‑TAAAAAACAGGTGACGATTCCGCTGGATACTCTGCTCACCAAACATGATCGCGTAACCGTACTGGGTACGTTCATTATGCTCGCAACCTATCAGCTGTTGTCCATCATGACTGTCTTCTCTATGACCTTTACTACCGCCTCCGCGACAGTGGGCCTTCGCCTGCCGCTTTCCGAAGTGTTGTAGCTTTTGATGAGCGCACCTATTGGCTCTGGCGGCATGGTGCCAg              >  2:285488/1‑239 (MQ=255)
                                                                                                                                                                                                                               aGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAg              >  2:334338/1‑239 (MQ=255)
                                                                                                                                                                                                                                  cGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCg           <  1:1121884/239‑1 (MQ=255)
                                                                                                                                                                                                                                     tAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCGCTg        <  1:394530/239‑1 (MQ=255)
                                                                                                                                                                                                                                            gAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCGCTGGATTa   <  2:1211038/238‑1 (MQ=255)
                                                                                                                                                                                                                                             aaaaaaCAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCGCTGGAtt    >  2:1256526/1‑236 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   
CCGCGCAAACGTGCACTGTATGGCTCCTTTCCGCAGCTGGGCGCACCGATTGGCTTCTTCTTTGCCAATGGCACTTTCTTGCTGCTTTCCTGGCTACTGACCGACGAGCAGTTTATGAGCTGGGGCTGGCGTGTGCCATTTATCTTCTCGGCGGTGCTGGTCATTATCGGCCTGTATG‑TTCGCGTG‑TCG‑CTGCA‑TGAGTCGCC‑GGTG‑TT‑T‑GAGAAAGTCGCTAAAGC‑GAAAAAACAGGTGAAGATCCCGCTGGGTACGCTGCTGACCAAACATGTTCGCGTAACCGTACTGGGTACGTTCATTATGCTGGCAACCTATACGCTGTTTTACATCATGACGGTCTACTCTATGACCTTTAGTACCGCCGCCGCGCCAGTTGGGCTTGGCCTGCCGCGTAACGAAGTGTTGTGGATGTTGATGATGGCAGTTATTGGTTTTGGCGTGATGGTGCCAGTCGCTGGATTAC  >  NC_000913/3675250‑3675715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A